Journal
MITOCHONDRIAL DNA PART A
Volume 27, Issue 5, Pages 3757-3758Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/19401736.2015.1079892
Keywords
Chloroplast genome; Fritillaria unibracteata var. wabuensis; single-molecule real-time (SMRT) technology
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Funding
- PUMC Youth Fund
- Fundamental Research Funds for the Central Universities [3332015144]
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Fritillaria unibracteata var. wabuensis is an important medicinal plant used for the treatment of cough symptoms related to the respiratory system. The chloroplast genome of F. unibracteata var. wabuensis (GenBank accession no. KF769142) was assembled using the PacBio RS platform (Pacific Biosciences, Beverly, MA) as a circle sequence with 151 009 bp. The assembled genome contains 133 genes, including 88 protein-coding, 37 tRNA, and eight rRNA genes. This genome sequence will provide important resource for further studies on the evolution of Fritillaria genus and molecular identification of Fritillaria herbs and their adulterants. This work suggests that PacBio RS is a powerful tool to sequence and assemble chloroplast genomes.
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