Journal
ARCHIVES OF VIROLOGY
Volume 164, Issue 2, Pages 359-370Publisher
SPRINGER WIEN
DOI: 10.1007/s00705-018-4071-8
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Funding
- Kansas National Bio and Agro-Defense Facility (NBAF) Transition Fund
- Department of Homeland Security Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD) [2010-ST061-AG0001]
- Kansas Biosciences Authority/CEEZAD matching funds [BG2428]
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In this study, an alphavirus vector platform was used to deliver replicon particles (RPs) expressing African swine fever virus (ASFV) antigens to swine. Alphavirus RPs expressing ASFV p30 (RP-30), p54 (RP-54) or pHA-72 (RP-sHA-p72) antigens were constructed and tested for expression in Vero cells and for immunogenicity in pigs. RP-30 showed the highest expression in Vero cells and was the most immunogenic in pigs, followed by RP-54 and RP-sHA-p72. Pigs primed with two doses of the RP-30 construct were then boosted with a naturally attenuated ASFV isolate, OURT88/3. Mapping of p30 identified an immunodominant region within the amino acid residues 111-130. However, the principal effect of the prime-boost was enhanced recognition of an epitope covered by the peptide sequence 61-110. The results suggest that a strategy incorporating priming with a vector-expressed antigen followed by boosting with an attenuated live virus may broaden the recognition of ASFV epitopes.
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