Article
Fisheries
Thuy-Dung Mai-Hoang, Hai-Ly Tien, Hai-Minh Chau-Hoang, Khai-Hoan Nguyen-Phuoc, Hung Quoc Pham, Thuoc Linh Tran, Hieu Tran-Van
Summary: AHPND is a global threat, especially for shrimp culture in Vietnam, caused by Vibrio parahaemolyticus carrying toxins with a mortality rate of up to 100% post infection. A mutant AHPND with a transposon insertion was recorded, but currently there is no commercially available PCR test to detect both normal and mutant plasmids simultaneously.
Article
Immunology
Hung N. Mai, Luis Fernando Aranguren Caro, Roberto Cruz-Flores, Brenda Noble White, Arun K. Dhar
Summary: A selected genetic line of Penaeus vannamei was found to be tolerant to Acute Hepatopancreatic Necrosis Disease (AHPND), and differential gene expression analysis between susceptible and tolerant shrimp populations revealed potential candidate markers for AHPND tolerance.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Food Science & Technology
Vikash Kumar, Suvra Roy, Bijay Kumar Behera, Peter Bossier, Basanta Kumar Das
Summary: Shrimp, a high-protein animal food commodity, is a fast-growing food producing sector globally. Disease outbreaks, particularly acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio spp., have led to significant production losses and socio-economic and environmental challenges in the shrimp aquaculture industry. Current conventional approaches to mitigate AHPND, such as antibiotics and disinfectants, have shown limited success and have been linked to antibiotic resistance in bacterial pathogens.
Article
Veterinary Sciences
Chenglong Li, Nan Lin, Zhihua Feng, Minhua Lin, Biyun Guan, Kunsen Chen, Wangwang Liang, Qiaohuang Wang, Miaomiao Li, Yu You, Qi Chen
Summary: A new molecular assay combining RPA and CRISPR/Cas12a technology was developed to detect the virulence genes causing AHPND in shrimp. The assay showed rapid and specific detection, with the addition of a lateral flow strip readout for visualization. Field tests demonstrated high predictive agreement.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Fisheries
Thao Nguyen, Andrea Alfaro, Bonny Bayot Arroyo, Jenny Antonia Rodriguez Leon, Stanislaus Sonnenholzner
Summary: The study used a metabolomics approach to measure molecular responses of shrimp exposed to Vibrio parahaemolyticus, revealing an increase in metabolic rate, elevated TCA cycle, and gluconeogenesis activities in infected shrimp. This provides important new findings about the pathogenic mechanisms of V. parahaemolyticus invasion in shrimp at the metabolic level.
Article
Fisheries
Bo Guo, Lihong Yang, Yu Wang, Chenjie Zhao, Xue Zhang, Yixin Tang, Yue Wang, Hui Shen, Song Gao, Pei Wang
Summary: By combining Pyrococcus furiosus Argonaute (PfAgo) with recombinase polymerase amplification (RPA), a method for fast and ultrasensitive diagnosis of acute hepatopancreas necrosis disease (AHPND) has been established. The procedure has a sensitivity of one copy per reaction and is suitable for point-of-care testing (POCT), making it valuable for disease prevention in shrimp farms.
Article
Microbiology
Ramya Kumar, Teng-Chun Tung, Tze Hann Ng, Che-Chih Chang, Yi-Lun Chen, Yi-Min Chen, Shih-Shun Lin, Han-Ching Wang
Summary: The study revealed that AHPND infection can impact lipid metabolism in shrimp stomach, and that the primary bile acid taurocholate is crucial for the virulence of AHPND-causing Vibrio parahaemolyticus.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Fisheries
Paul J. Schofield, Brenda L. Noble, L. Fernando Aranguren Caro, Hung N. Mai, Tanner J. Padilla, Jasmine Millabas, Arun K. Dhar
Summary: Penaeus vannamei shrimp are more susceptible to Acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus, while Macrobrachium rosenbergii are not affected at salinities lower than 20 ppt.
AQUACULTURE RESEARCH
(2021)
Article
Environmental Sciences
Orapint Jintasataporn, Srinoy Chumkam, Supawit Triwutanon, Allan LeBlanc, Jarin Sawanboonchun
Summary: The efficacy of methanotroph bacteria meal as a fishmeal replacement in Pacific white shrimp diets was studied, showing no adverse effects on growth, feed utilization, and survival rate. Shrimp fed methanotroph bacteria meal diets exhibited improved survival rates against an AHPND challenge.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Fisheries
K. G. Aguilar-Rendon, S. A. Soto-Rodriguez, B. Gomez-Gil, R. Lozano-Olvera, B. Yanez-Rivera
Summary: This study investigated the impact of pathogenic strains causing acute hepatopancreatic necrosis disease (AHPND) on the water microbiome. The results showed that the virulence of the strains and the duration of the experiment could alter the water microbiome, particularly leading to a significant reduction in the reads assigned to Neptuniibacter spp. This depletion suggests a possible dual function of the type VI secretion system 1 (T6SS1) in the pathogenic strains, killing surrounding bacteria and damaging the hepatopancreas.
Article
Fisheries
Punyaporn Naranitus, Praphutson Aiamsa-At, Thanwarat Sukonta, Pimkhuan Hannanta-Anan, Thawatchai Chaijarasphong
Summary: In this study, two fluorescent assays compatible with naked-eye visualization were developed using CRISPR-Cas12a technology. These assays, based on PCR and RPA techniques, demonstrated high sensitivity and specificity within less than an hour and can be read using a portable smartphone device.
JOURNAL OF FISH DISEASES
(2022)
Article
Biotechnology & Applied Microbiology
Eduardo Quiroz-Guzman, Monica Cabrera-Stevens, Arturo Sanchez-Paz, Fernando Mendoza-Cano, Trinidad Encinas-Garcia, Diana Barajas-Sandoval, Bruno Gomez-Gil, Alberto Pena-Rodriguez
Summary: This study evaluated the effects of four functional diets and a reference diet on the survival and intestinal bacterial community of shrimps infected with AHPND. The results showed that diets containing TuMa and VitC significantly increased shrimp survival rate. TuMa diet reduced Vibrionales, while VitC diet increased Alteromonadales. The findings suggest that functional diets can protect against AHPND by promoting a desirable bacterial community in the intestine.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Fisheries
Xiaoqian Gu, Baojie Wang, Keyong Jiang, Mei Liu, Lei Wang
Summary: This study revealed the role of Litopenaeus vannamei fatty acid binding protein (LvFABP) in the resistance against acute hepatopancreatic necrosis disease (AHPND). LvFABP was found to be abundant in the cytoplasm of hepatopancreas cells and interacted with the PirBvp toxin. Antibodies against LvFABP partially blocked the binding of LvFABP to PirBvp in vitro and inhibited AHPND infection in vivo. LvFABP had a neutralizing effect against AHPND and protected L. vannamei from morphological damage caused by the pathogen. Silencing LvFABP protected metabolic homeostasis and enabled adaptation to further or prolonged AHPND infections.
Article
Fisheries
T. H. Noble, M. Rao, M. Briggs, A. P. Shinn, C. Simon, J. W. Wynne
Summary: This study demonstrates that feeding shrimp Novacg (TM) improves survival against VPAHPND infection, with significantly different survival estimates between challenge trials. There were no significant differences in pir toxin gene expression or V. parahaemolyticus abundance between the two diet treatments, but both toxin expression and V. parahaemolyticus abundance changed significantly throughout the disease course.
Article
Biochemical Research Methods
Hung Nam Mai, Luis F. Aranguren Caro, Roberto Cruz-Flores, Arun K. Dhar
Summary: AHPND, caused by Vibrio spp. carrying pirA and pirB genes, is a major bacterial disease in shrimp industry, diagnosed mainly by PCR but limited for point-of-care diagnostics. An isothermal RPA assay was developed for AHPND detection, showing high specificity and sensitivity with a detection limit of 5 copies of pirAB genes, making it a reliable alternative method for AHPND detection outside a standard laboratory setup.
MOLECULAR AND CELLULAR PROBES
(2021)
Article
Fisheries
Hunter S. Bailey, Ashley N. Fincannon, Lee A. Fuiman
Summary: This study investigated the transfer of fatty acids from broodstock diets to eggs and the time needed for the eggs to equilibrate to the diet in Southern flounder. The findings indicate that different diet treatments significantly affect the fatty acid composition of eggs, and it takes 8-16 weeks for the eggs to adapt to changes in the adult diet. These results are important for improving broodstock diets and feeding protocols in stock-enhancement programs.
Article
Fisheries
Yu Liu, Mingtao Lei, Hector Victor, Yan Wang
Summary: This study confirms the feasibility of replacing raw fish diet with a formulated diet in commercial farming of large yellow croaker, which can increase fish survival and yield, and reduce feed cost and body lipid deposition.
Article
Fisheries
Xiaoyan Zhang, Lize San, Yucong Yang, Yuehong Tao, Jiangong Ren, Yufeng Liu, Zhongwei He, Jiashuo Di, Ziteng Pei, Guixing Wang, Jilun Hou
Summary: Gynogenesis is an effective technique for establishing homogeneous lines and confirming potential chromosomal mechanisms of sex determination in fish. This study successfully induced gynogenetic families in Thamnaconus modestus and established a complete method for gynogenesis induction.
Article
Fisheries
Julieta C. Martinelli, Megan Considine, Helen R. Casendino, Carolyn M. Tarpey, Isadora Jimenez-Hidalgo, Jacqueline L. Padilla-Gamino, Teri L. King, Lorenz Hauser, Steven Rumrill, Chelsea L. Wood
Summary: Shell-boring polychaetes have caused significant damage to mariculture operations worldwide, particularly in the US Pacific region. This study provides a comprehensive dataset on the prevalence, species distribution, and environmental associations of these parasites, highlighting the impact of season, culturing methods, and environmental factors on infestation.
Article
Fisheries
Juan Gao, Xueshan Li, Kangle Lu, Kai Song, Jian Zhang, Ling Wang, Chunxiao Zhang
Summary: This study investigated the effects of dietary protein levels on growth performance, antioxidant capacity, digestive enzyme activity, and metamorphosis rate of bullfrog tadpoles. The optimal dietary protein level for bullfrog tadpoles was estimated to be 42.49%-46.50% of the diet.
Article
Fisheries
Qiang Li, Lijun Duan, Dongsheng Jin, Yuxin Chen, Yirong Lou, Qianjin Zhou, Zhongjie Xu, Fangjie Chen, Hongxian Chen, Guizong Xu, Maocang Yan, Guanjun Yang, Jianfei Lu, Yanjun Zhang, Jiong Chen
Summary: This study developed a centrifugal microfluidic chip with on-chip RPA to detect five pathogenic microorganisms. The chip enabled the parallel analysis of six genetic markers from a single sample and allowed for the highly automated detection of multiple samples. Compared with PCR and DNA sequencing, the on-chip RPA assay showed high sensitivity and specificity for detecting multiple pathogens in shrimp aquaculture.
Article
Fisheries
Xingchen Huo, Pengxu Wang, Fengxia Zhao, Qian Liu, Qingqing Tian, Lingjie Tang, Maolin Lv, Zhaohui Wei, Chunrong Yang, Jianguo Su
Summary: Bacterial diseases in aquaculture ponds have negative effects on sustainable development. The use of composite I20 biotherapy has been shown to effectively treat bacterial diseases in bullfrog ponds, providing a new strategy for controlling bacterial diseases in aquaculture.
Article
Fisheries
Peng Yin, Takaya Saito, Per Gunnar Fjelldal, Bjorn Tharandur Bjornsson, Sofie Charlotte Remo, Sandeep Sharma, Rolf Erik Olsen, Kristin Hamre
Summary: This study aims to investigate the effect of seasonal changes in temperature and photoperiod on the redox metabolism of Atlantic salmon at the molecular level. The research findings suggest that the changing photoperiod and temperature influenced the redox metabolism of Atlantic salmon, highlighting the importance of oxidative status in the ecological implications.
Article
Fisheries
Nicholas Oppong Mensah, Jeffery Kofi Asare, Emmanuel Tetteh-Doku Mensah, Ernest Christlieb Amrago, Frank Osei Tutu, Anthony Donkor
Summary: This study investigates aquaculture farmers' preference for climate-smart aquaculture insurance products, the challenges they face, and their preferred insurance coverage. The results show that farmers prefer Climate-Induced Aquaculture Stock Mortality Insurance and the most significant constraint is the delay in claim settlement.
Article
Fisheries
Qiu-Ping Chai, Pei Wu, Wei-Dan Jiang, Yang Liu, Hong-Mei Ren, Xiao-Wan Jin, Lin Feng, Xiao-Qiu Zhou
Summary: The study found that appropriate levels of potassium diformate (KDF) can enhance the immune defense and mediate the inflammatory process in fish, possibly through the regulation of T cell differentiation via JAK-STAT and NF-kappa B signaling pathways.
Article
Fisheries
Tian Zhu, Haomin Jia, Haopeng Zhang, Yujing Xiao, Cui Han, Jiaxin Yang
Summary: Chicken manure has significant effects on the cultivation of Chlorella and rotifers. The optimal amount of chicken manure extract for continuous cultivation is 10 mLL-1. Chicken manure can promote the growth of Chlorella and improve the fatty acid composition, indirectly enhancing the growth condition and fatty acid content of rotifers.
Article
Fisheries
Zheng Luo, Yang Yu, Zhenning Bao, Fuhua Li
Summary: This study analyzed the heritability and genetic correlation of two growth traits in Pacific white shrimp and evaluated the genomic prediction using different genomic selection models. The results showed that the NeuralNet model had the highest prediction accuracy and better prospects for predicting shrimp growth traits.
Article
Fisheries
Alberto Ruiz, Ignasi Sanahuja, Karl B. Andree, Dolors Furones, Paul G. Holhorea, Josep A. Calduch-Giner, Jose J. Pastor, Marc Vinas, Jaume Perez-Sanchez, Sofia Morais, Enric Gisbert
Summary: Supplementing diets with a combination of spice oleoresins can reduce lipid accumulation and inflammation in farmed fish. The combination of spice oleoresins also has an immunomodulatory effect on the fish's intestinal immune system, potentially offering anti-inflammatory benefits.
Article
Fisheries
Beibei Zhao, Renhui Mei, Le Li, Di Hu, Lei Li
Summary: This study reveals that JfCXCL8_L1a and JfCXCL8_L1b have different immune pathways, and JfCXCL8_L1b plays a significant role in enhancing the adaptive immunity of T cell-dependent antigen.
Article
Fisheries
Yuhang Liu, Danying Cao, Nan Wu, Xuyang Zhao, Qingsong Zhu, Lian Su, Fatima Altaf, Qianqian Zhang, Haokun Liu, Yongming Li, Bruno Hamish Unger, Yingyin Cheng, Wanting Zhang, Aihua Li, Yaping Wang, Xiao-Qin Xia
Summary: Based on previous research, sinomenine has been found to protect mucosal immunity in farmed fish species by preventing intestinal pathological changes and regulating gene expression related to inflammation. It also enhances immune homeostasis and controls the growth of pathogenic bacteria.
