Article
Microbiology
Chaofang Zhong, Bingpeng Qu, Gang Hu, Kang Ning
Summary: This study revealed the pan-genome of 39 Campylobacter species, provided the most updated reconstruction of the global virulence gene pool of 39 Campylobacter species, and identified species-related virulence differences.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Radwa Abdelwahab, Munirah M. Alhammadi, Ehsan A. Hassan, Entsar H. Ahmed, Nagla H. Abu-Faddan, Enas A. Daef, Stephen J. W. Busby, Douglas F. Browning
Summary: Klebsiella pneumoniae is an important human pathogen in Egypt with high levels of antibiotic resistance, and strain KPE16 isolated from a child in Assiut carries multiple antimicrobial resistance genes, including a bla(NDM-1) carbapenemase and extended spectrum beta-lactamase genes. Comparison with other Egyptian isolates reveals common plasmids and resistance genes, suggesting a potential source of antibiotic resistance worldwide.
Article
Infectious Diseases
Andrey Shelenkov, Lyudmila Petrova, Mikhail Zamyatin, Yulia Mikhaylova, Vasiliy Akimkin
Summary: The study identified five international clones and three isolates not belonging to major clones in a multidisciplinary medical center during 2017-2019. Whole-genome sequencing of nine representative clinical Acinetobacter baumannii isolates was performed to determine antibiotic resistance and virulence genes, reveal CRISPR/Cas systems, obtain plasmid structures, compare phenotypic and genotypic antibiotic resistance profiles, and discuss possible ways of isolate and resistance spreading. The data obtained will contribute to a better understanding of the spread and resistance acquisition of Acinetobacter baumannii international clones, highlighting the importance of continuous genomic epidemiology surveillance of this pathogenic bacterial species.
Article
Infectious Diseases
Md Maidul Islam, Jeongah Kim, Kyeongmin Kim, Dooyoung Kim, Samia Akter, Seunghyeok Bang, Shukho Kim, Jungmin Kim, Je Chul Lee, Minsang Shin
Summary: This study evaluates the genomic features of clinical strain A. baumannii KBN10P05679 to elucidate its antibiotic resistance mechanisms and virulence factors. The results show that KBN10P05679 strain has a higher capacity for biofilm formation and expresses biofilm-related genes at a higher level.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Genetics & Heredity
Romulo Maia Ferreira, Douglas Henrique dos Santos Silva, Karinny Farias Silva, Joveliane de Melo Monteiro, Gabriella Freitas Ferreira, Maria Raimunda Chagas Silva, Luis Claudio Nascimento da Silva, Leticia de Castro Oliveira, Andrea Souza Monteiro
Summary: This study sequenced the genome of a multidrug-resistant strain of Staphylococcus aureus (SA01) and analyzed the genes associated with drug resistance and virulence. The results revealed that SA01 showed resistance to multiple antibiotics and possessed several virulence-associated genes. These findings are significant for monitoring and controlling the spread of SA01.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Chemistry, Medicinal
Tabbetha J. Bohac, Luting Fang, Victoria S. Banas, Daryl E. Giblin, Timothy A. Wencewicz
Summary: The synthetic siderophores can act as versatile structural and biomimetic analogues of native siderophores in targeting the pathogenic Gram-negative human pathogen Acinetobacter baumannii, with potential therapeutic applications as chemical probes or drugs. The metal-free and ferric iron complexes of the synthetic siderophores promote growth of A. baumannii, while the Ga(III)-complexes inhibit its growth effectively with low MIC values. Additionally, the synthetic siderophores compete with native siderophores for uptake in A. baumannii and maintain comparable binding affinities for ferric iron and siderophore-binding protein BauB, offering new insights for the development of siderophore-based therapeutics targeting pathogenic strains of A. baumannii.
ACS INFECTIOUS DISEASES
(2021)
Article
Immunology
Jia Xiao, Yiluo Cheng, Wenting Zhang, Qin Lu, Yunqing Guo, Qiao Hu, Guoyuan Wen, Huabin Shao, Qingping Luo, Tengfei Zhang
Summary: This study investigated the prevalence and antibiotic resistance of Campylobacter in local Chinese dual-purpose chickens. The dominant clonal complexes of C. jejuni and C. coli were identified, and C. coli exhibited a higher antibiotic resistance rate than C. jejuni. Whole-genome sequencing detected multiple antibiotic resistance genes, but the correlation between genes and resistance phenotypes was weak for most antibiotics. Additionally, virulence gene analysis revealed differences between C. jejuni and C. coli in genes related to adhesion, colonization, and invasion.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Xiaoqing Wang, Belinda Loh, Fernando Gordillo Altamirano, Yunsong Yu, Xiaoting Hua, Sebastian Leptihn
Summary: Multidrug-resistant bacterial infections are on the rise, leading to the search for new therapeutic approaches. Phage therapy is gaining attention as an alternative treatment strategy, but faces the challenge of phage-resistant bacteria. This study aims to understand the mechanisms of phage resistance in Acinetobacter baumannii and identifies potential strategies that could be exploited in clinical settings.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Biotechnology & Applied Microbiology
Melanie Grosjean, Sophie Guenard, Caroline Giraud, Cedric Muller, Patrick Plesiat, Paulo Juarez
Summary: Pseudomonas aeruginosa is commonly used to study antibiotic resistance and pathogenesis, but its natural resistance to antibiotics and ability to produce virulence factors hinder its biotechnological potential. By reducing the genome of this bacterium, researchers developed hypovirulent and hypersusceptible recombinant DNA hosts, laying the foundation for further optimization for biotechnological applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Nadya Rakovitsky, Mor N. Lurie-Weinberger, Amichay Hameir, Liat Wulffhart, Alona Keren Paz, David Schwartz, Yehuda Carmeli
Summary: Acinetobacter baumannii has been identified as a major healthcare threat, with its pathogenesis and molecular mechanism of virulence poorly understood. In this study, researchers identified and characterized nine string-positive CRAB (stCRAB) isolates, finding that unlike Klebsiella pneumoniae, stCRAB isolates were not associated with increased virulence.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Sarah K. Giles, Uwe H. Stroeher, Bhavya Papudeshi, Robert A. Edwards, Jessica A. P. Carlson-Jones, Michael Roach, Melissa H. Brown
Summary: This study reveals the complexity of colistin resistance in Acinetobacter baumannii, with multiple levels of regulation involved. The up-regulation of the pmrCAB operon, which is associated with the StkSR two-component system, is identified as one of the mechanisms contributing to increased colistin resistance in the Delta stkR strain.
Article
Immunology
Shital N. Kumkar, Ekta E. Kamble, Nikeeta S. Chavan, Dhiraj P. Dhotre, Karishma R. Pardesi
Summary: This study investigated the whole genome sequences of A. baumannii isolates from India and provided insights into their resistome, virulome, and mobilome. The analysis revealed a larger number of A. baumannii isolates belonging to the ST2 genotype and showed genetic diversity between compared genomes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Immunology
Chunli Sun, Yunsong Yu, Xiaoting Hua
Summary: Acinetobacter baumannii is a common pathogen causing various infectious diseases and has a high drug resistance rate, severely limiting antibiotic treatment options. Tigecycline and polymyxins are considered the last line of defense against multidrug resistant A. baumannii. This review focuses on the mechanisms of tigecycline resistance in A. baumannii and highlights the need for further investigation and development of new antibiotics.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Sandra Sanchez-Urtaza, Alain Ocampo-Sosa, Ainhoa Molins-Bengoetxea, Mohammed A. A. El-Kholy, Marta Hernandez, David Abad, Sherine M. M. Shawky, Itziar Alkorta, Lucia Gallego
Summary: This study investigated the genetic characteristics and antimicrobial susceptibility profile of multidrug resistant Acinetobacter baumannii clinical isolates obtained from hospitals in Alexandria, Egypt. The results showed resistance to multiple antibiotics and the presence of various antibiotic resistance genes and virulence factors. The study highlights the importance of investigating molecular epidemiology to control the spread of these multidrug resistant isolates.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Karen Ross, Alessandro M. Varani, Erik Snesrud, Hongzhan Huang, Danillo Oliveira Alvarenga, Jian Zhang, Cathy Wu, Patrick McGann, Mick Chandler
Summary: TnCentral is a web resource dedicated to prokaryotic transposable elements (TE), providing detailed annotation and information on approximately 400 TE from various families. Users can browse and query the content through text- and sequence-based searches, and there are downloadable software tools available for user-driven identification and manual annotation of TE in genomic sequences.
Article
Infectious Diseases
Francesc Escrihuela-Vidal, Achim J. Kaasch, Maja Von Cube, Siegbert Rieg, Winfried V. Kern, Harald Seifert, Kyoung-Ho Song, Chun-Hsing Liao, Robert Tilley, Hannah Gott, Matt Scarborough, Claire Gordon, Martin J. Llewelyn, Richard Kuehl, Laura Morata, Alex Soriano, Jonathan Edgeworth, Enrique Ruiz De Gopegui, Emmanuel Nsutebu, Jose Miguel Cisneros, Vance G. Fowler, Guy Thwaites, Joaquin Lopez-Contreras, Gavin Barlow, Hugo Guillermo Ternavasio-De La Vega, Jesus Rodriguez-Bano, Luis Eduardo Lopez-Cortes
Summary: This study analysed the adherence and impact of quality-of-care indicators (QCIs) in the management of Staphylococcus aureus bloodstream infection. The results showed that adherence to QCIs was associated with reduced 90-day mortality.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Infectious Diseases
Anna M. Rohde, Sarah Walker, Michael Behnke, Simone Eisenbeis, Linda Falgenhauer, Jane C. Falgenhauer, Georg Haecker, Florian Hoelzl, Can Imirzalioglu, Nadja Kaeding, Winfried Kern, Axel Kola, Evelyn Kramme, Alexander Mischnik, Silke Peter, Siegbert Rieg, Jan Rupp, Christian Schneider, Frank Schwab, Harald Seifert, Evelina Tacconelli, David Tobys, Janina Trauth, Anna Weber, Kyriaki Xanthopoulou, Janine Zweigner, Paul G. Higgins, Petra Gastmeier
Summary: This study assessed the prevalence of vancomycin-resistant Enterococcus faecium (VREfm) in German hospitals upon admission and analyzed the risk factors for colonization. The results showed that age, healthcare contacts, antibiotic use, and PPI/antacid consumption increased the individual risk of VREfm colonization. The study also found that the admission prevalence of VREfm increased each year, mainly driven by the emergence of the ST117 strain.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Infectious Diseases
Alexander Wunderlich, Kyriaki Xanthopoulou, Julia Wille, Esther Wohlfarth, Stefanie Gerson, Martin Kaase, Harald Seifert, Paul G. Higgins
Summary: This study aimed to characterize the genetic environment of metallo-beta-lactamases (MBL) in carbapenem-resistant clinical Acinetobacter pittii isolates. The results showed the existence of a promiscuous plasmid circulating in A. pittii isolates in Germany with high similarity in the plasmid backbone but differences in the MBL-encoding region.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Florent Kempf, Guido Cordoni, Anne-Marie Chausse, Rosanna Drumo, Helen Brown, Daniel L. Horton, Frederic Paboeuf, Martine Denis, Philippe Velge, Roberto La Ragione, Annaelle Kerouanton
Summary: By analyzing immune-associated markers and gut microbiota composition in pigs infected with Salmonella, we found that identifying high shedder pigs can help mitigate the risk of pathogen spread in the food chain.
Article
Medicine, General & Internal
Adi Turjeman, Elodie von Dach, Jose Molina, Erica Franceschini, Fidi Koppel, Dana Yelin, Yael Dishon-Benattar, Cristina Mussini, Jesus Rodriguez-Bano, Jose Miguel Cisneros, Angela Huttner, Mical Paul, Leonard Leibovici, Dafna Yahav
Summary: This study aims to compare the effect of short and long-term treatment in Gram-negative bacteremia on all-cause mortality in pre-specified subgroups. The results show that in patients with stable hemodynamics and normal temperature for 48 hours prior to discontinuation, seven days of antibiotic therapy for enterobacterales bacteremia result in similar outcomes as 14 days.
Article
Infectious Diseases
Andrea Vila Dominguez, Marta Carretero Ledesma, Carmen Infante Dominguez, Jose Miguel Cisneros, Jose A. A. Lepe, Younes Smani
Summary: The Lm CC388 strain of Listeria monocytogenes was responsible for a severe outbreak in southern Spain in 2019, resulting in three deaths. This strain exhibited higher virulence compared to other highly virulent clones in both in vitro and in vivo experiments.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Infectious Diseases
Jose Manuel Ortiz de la Rosa, Maria Aznar Fernandez, Angel Rodriguez-Villodres, Carlos S. Casimiro-Soriguera, Jose Miguel Cisneros, Jose Antonio Lepe
Summary: Delafloxacin is a new fluoroquinolone approved for the treatment of complicated bacterial skin infections caused by Staphylococcus aureus. However, resistance to delafloxacin has been reported in some strains of S. aureus. This study investigated the activity of delafloxacin against a collection of S. aureus isolates and identified key mechanisms for the development of delafloxacin resistance.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Microbiology
Sonja Mertins, Paul G. Higgins, Caroline Thunissen, Henri Magein, Quentin Gilleman, Pascal Mertens, Maria Gonzalez Rodriguez, Liza Marie Maus, Harald Seifert, Martin Kroenke, Alexander Klimka
Summary: There is a need for a rapid detection method for carbapenem-resistant Acinetobacter baumannii (CRAb) to enable appropriate antimicrobial treatment and prevent transmission. This study aimed to expand the OXA-detection abilities of a rapid immunochromatographic test (ICT) to include OXA-40- and OXA-58-like carbapenemases, which confer carbapenem resistance to the majority of CRAb isolates worldwide.
JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Editorial Material
Immunology
Carolina Silva Nodari, Andres Opazo-Capurro, Santiago Castillo-Ramirez, Vittoria Mattioni Marchetti
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Ana Casado, Adelina Gimeno, Manuela Aguilar-Guisado, Manuel Garcia, Jesus Francisco Rodriguez, Pedro Antonio Rivas, Claudio Bueno, Jose Antonio Lepe, Jose Miguel Cisneros, Jose Molina, Pranita D. Tamma
Summary: This study evaluates the safety of early oral ambulatory treatment for adult patients diagnosed with bacteremia after discharge from the emergency department. A cohort of 206 febrile ambulatory patients was assessed. Bacteremic low-risk patients were recommended oral treatment and were compared with matched febrile non-bacteremic outpatients. Rates of 14-day mortality and unplanned re-consultations were similar and below 5% in both cohorts, highlighting the safety of oral therapy for low-risk bacteremia, even from its onset.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Letter
Infectious Diseases
Dhammika Leshan Wannigama, Mohan Amarasiri, Phatthranit Phattharapornjaroen, Cameron Hurst, Charin Modchang, Sudarat Chadsuthi, Suparinthon Anupong, Kazuhiko Miyanaga, Longzhu Cui, Stefan Fernandez, Angkana T. Huang, Puey Ounjai, Ratana Tacharoenmuang, Naveen Kumar Devanga Ragupathi, Daisuke Sano, Takashi Furukawa, Kazunari Sei, Asada Leelahavanichkul, Talerngsak Kanjanabuch, Paul G. Higgins, Asuka Nanbo, Anthony Kicic, Andrew C. Singer, Tanittha Chatsuwan, Sam Trowsdale, Aisha Khatib, Kenji Shibuya, Shuichi Abe, Hitoshi Ishikawa, Parichart Hongsing
LANCET INFECTIOUS DISEASES
(2023)
Article
Microbiology
Carina Mueller, Sandra Reuter, Julia Wille, Kyriaki Xanthopoulou, Danuta Stefanik, Hajo Grundmann, Paul G. Higgins, Harald Seifert
Summary: This study conducted molecular epidemiology and global distribution research on 313 carbapenem-resistant Acinetobacter baumannii isolated from 114 study centers in 47 countries. The results revealed a wide variation in the distribution of carbapenemase encoding genes among different geographical regions.
Correction
Biochemistry & Molecular Biology
Aurore C. Poirier, Ruben D. Riano Moreno, Leona Takaindisa, Jessie Carpenter, Jai W. Mehat, Abi Haddon, Mohammed A. Rohaim, Craig Williams, Peter Burkhart, Chris Conlon, Matthew Wilson, Matthew Mcclumpha, Anna Stedman, Guido Cordoni, Manoharanehru Branavan, Mukunthan Tharmakulasingam, Nouman S. Chaudhry, Nicolas Locker, Anil Fernando, Wamadeva Balachandran, Mark Bullen, Nadine Collins, David Rimer, Daniel L. Horton, Muhammad Munir, Roberto M. La Ragione
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Aurore C. Poirier, Ruben D. Riano Moreno, Leona Takaindisa, Jessie Carpenter, Jai W. Mehat, Abi Haddon, Mohammed A. Rohaim, Craig Williams, Peter Burkhart, Chris Conlon, Matthew Wilson, Matthew Mcclumpha, Anna Stedman, Guido Cordoni, Manoharanehru Branavan, Mukunthan Tharmakulasingam, Nouman S. Chaudhry, Nicolas Locker, Anil Fernando, Wamadeva Balachandran, Mark Bullen, Nadine Collins, David Rimer, Daniel L. Horton, Muhammad Munir, Roberto M. La Ragione
Summary: This study presents a rapid and inexpensive diagnostic platform for SARS-CoV-2 (COVID-19) using the RT-LAMP assay and a portable smart diagnostic device. The platform utilizes automated image acquisition and an AI deep learning model to eliminate subjectivity in result interpretation. The AI-assisted diagnostics platform showed high specificity and sensitivity compared to the gold standard RT-qPCR test.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Microbiology
Tara Davis, Dagmara Bialy, Joy Leng, Roberto La Ragione, Holly Shelton, Klaudia Chrzastek
Summary: The diversity of gut and respiratory microbiota in chickens is related to H9N2 avian influenza virus (AIV) infection. In the acute phase of infection, the diversity of respiratory microbiota decreases, and it does not fully recover to normal levels in the recovery phase after infection. Lactobacillus is associated with the microbiota of infected chickens at different stages of infection. Additionally, the respiratory microbiota in chickens undergoes age-related changes during maturation.