Article
Andrology
Michal Novoselsky Persky, Anat Hershko-Klement, Amy Solnica, Yuval Bdolah, Arye Hurwitz, Mali Ketzin El Gilad, Iris Nefesh, Efrat Esh-Broder
Summary: This study compared the fertilization and embryo development between ICSI and PICSI in sibling oocytes, and found that PICSI significantly improved fertilization rates and rates of embryos eligible for transfer.
Article
Andrology
Paola Scaruffi, Francesca Bovis, Ida Casciano, Elena Maccarini, Caterina De Leo, Irene Gazzo, Claudia Massarotti, Fausta Sozzi, Sara Stigliani, Paola Anserini
Summary: Selection of HA-bound spermatozoa significantly improved clinical outcomes in couples with previous ICSI cycle failure, compared to further standard ICSI.
Article
Agriculture, Dairy & Animal Science
Sara Ruiz-Diaz, Rosane Mazzarella, Paula Navarrete-Lopez, Raul Fernandez-Gonzalez, Celia de Frutos, Maria Maroto, Claudia Cucala, Paula Beltran-Brena, Marta Lombo, Dimitrios Rizos, Alfonso Gutierrez-Adan
Summary: This study aimed to analyze if bull spermatozoa could be selected by thermotaxis and to assess their quality in terms of SDF as well as determine the presence of a specific sperm subpopulation based on sperm morphometry and assess their fertilizing capacity by ICSI. The results showed that thermotactic sperm selection led to higher DNA integrity, small and elongated head size parameters, and a different sperm subpopulation structure.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Maria Jose Gomez-Torres, Miranda Hernandez-Falco, Andrea Lopez-Botella, Natalia Huerta-Retamal, Paula Saez-Espinosa
Summary: This study describes the distribution of IZUMO1 in human sperm under different physiological conditions. The results show that IZUMO1 is mainly located in the acrosomal region, and a new distribution pattern suggests the migration of IZUMO1. The study also reveals various combinations between the IZUMO1 staining patterns and the acrosomal stages.
Article
Biophysics
Sai-Xi Yu, Yanan Liu, Yi Wu, Hao Luo, Rufei Huang, Ya-Jun Wang, Xuemei Wang, Hai Gao, Huijuan Shi, Guangyin Jing, Yan-Jun Liu
Summary: This study used hyaluronic acid as a substitute for cervical mucus and designed a cervix chip to study sperm selection and behavior. The results showed that hyaluronic acid can act as a filter to select active sperm.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Obstetrics & Gynecology
Robert West, Arri Coomarasamy, Lorraine Frew, Rachel Hutton, Jackson Kirkman-Brown, Martin Lawlor, Sheena Lewis, Riitta Partanen, Alex Payne-Dwyer, Claudia Roman-Montanana, Forough Torabi, Sofia Tsagdi, David Miller
Summary: What effects did treatment using hyaluronic acid (HA) binding/selection prior to ICSI have on clinical outcomes in the Hyaluronic Acid Binding sperm Selection (HABSelect) clinical trial? Older women randomized to the trial's experimental arm (selection of sperm bound to immobilized (solid-state) HA) had the same live birth rates as younger women, most likely a result of better avoidance of sperm with damaged DNA. Recent randomized controlled trials (RCTs) investigating the efficacy of HA-based sperm selection prior to ICSI, including HABSelect, have consistently reported reductions in the numbers of miscarriages among couples randomized to the intervention, suggesting a pathological sperm-mediated factor mitigated by prior HA-binding/selection. The mechanism of that protection is unknown.
HUMAN REPRODUCTION
(2022)
Review
Medicine, General & Internal
Kaan Aydos, Oya Sena Aydos
Summary: Retrieving spermatozoa from the testicles has been a great hope for patients with non-obstructive azoospermia (NOA), but relevant methods have not yet been developed to provide resolutions for all cases of NOA. In vitro selection and processing of quality sperm are essential for the success of in vitro fertilization. Sperm cryopreservation is widely used in assisted reproductive technologies, even for preserving very small numbers of sperm with advanced freezing and preserving techniques.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Obstetrics & Gynecology
Manar Hozyen, Eman Hasanen, Khaled Elqusi, Salma ElTanbouly, Samar Gamal, Abdul Ghafar Hussin, Hanaa AlKhader, Hosam Zaki
Summary: In patients with abnormal SDF undergoing ICSI treatment, the PICSI group showed the highest pregnancy rate while the DGC group had the lowest. The PICSI group also had a significantly higher implantation rate than the other groups, with the MACS and DGC groups performing better in terms of high-quality blastocyst rates.
REPRODUCTIVE SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Luis Andres Perez, Rebeca Hernandez, Jose Maria Alonso, Raul Perez-Gonzalez, Virginia Saez-Martinez
Summary: Hyaluronic acid (HA) hydrogels have a wide range of biomedical applications, and current research is focusing on developing methods for their production under physiological conditions. The review discusses the chemical reactions and properties involved, as well as the applications in tissue engineering and drug controlled release.
Review
Cell Biology
Domenico Baldini, Daniele Ferri, Giorgio Maria Baldini, Dario Lot, Assunta Catino, Damiano Vizziello, Giovanni Vizziello
Summary: In assisted reproductive technology (ART), the selection of sperm cells is crucial for fertilization potential, with intracytoplasmic sperm injection (ICSI) being the most widely used technique. Various methods have been developed to mimic natural selection processes, but doubts and disagreements on the most effective technique remain.
Article
Chemistry, Multidisciplinary
Damian A. Blois, Ana C. Liaudat, Virginia Capella, Gricelda Morilla, Rebeca E. Rivero, Martin F. Broglia, Cesar A. Barbero, Nancy Rodriguez, Pablo Bosch, Claudia R. Rivarola
Summary: The study explores the use of polymeric materials for sperm selection, finding a semi-interpenetrated hydrogel surface that allows for efficient sperm release with good motility and viability. The interaction between hydrogel surface and sperm is investigated, with potential applications in human and other mammalian species for advanced assisted reproductive techniques.
ADVANCED MATERIALS INTERFACES
(2021)
Article
Obstetrics & Gynecology
Yolanda Cabello, Pedro Belchin, Mercedes Gonzalez-Martinez, Carmen Lopez-Fernandez, Stephen Johnston, Jaime Gosalvez
Summary: The study investigated the effect of a novel non-centrifugation method (Io-Lix) on sperm parameters and ICSI reproductive outcomes. The results showed that Io-Lix sperm selection improved sperm parameters and reduced DNA fragmentation, leading to an increase in pregnancy rate and reduction in miscarriage in autologous ICSI. However, no significant difference was observed in the donor oocyte ICSI program.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Obstetrics & Gynecology
Maria Gil Julia, Irene Hervas
Summary: This retrospective multicentre observational study aimed to investigate the impact of magnetic-activated cell sorting (MACS) on clinical parameters and cumulative live birth rates (CLBR) in couples undergoing intracytoplasmic sperm injection (ICSI) with donor oocytes. The study found that the MACS group had a slightly higher CLBR per embryo transferred compared to the reference group, but this difference was not clinically significant. Therefore, the indiscriminate recommendation of MACS as a sperm processing add-on for all infertile patients undergoing ICSI with donated oocytes is not suggested.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Cell Biology
Naila Umer, Lena Arevalo, Sharang Phadke, Keerthika Lohanadan, Gregor Kirfel, Dominik Sons, Denise Sofia, Walter Witke, Hubert Schorle
Summary: Profilin (PFN) proteins play crucial roles in actin polymerization and are encoded by four Pfn genes in mice and humans. PFNs, particularly PFN3, are essential for spermiogenesis, sperm morphology, and fertility in males. Lack of PFN3 leads to abnormal acrosome formation, reduced sperm motility, and dysregulation of autophagy, ultimately impacting male reproductive function.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Obstetrics & Gynecology
Catherine Martin, Emma Woodland
Summary: Intracytoplasmic sperm injection (ICSI) is a commonly used in vitro fertilization technique, and physiological ICSI (PICSI) selects high-quality sperm using hyaluronic acid receptors. Other advanced sperm selection techniques like magnetic-activated cell sorting and Zeta potential sperm selection are also being studied, but currently lack sufficient evidence to confirm their clinical effectiveness.
SEMINARS IN REPRODUCTIVE MEDICINE
(2021)
Article
Public, Environmental & Occupational Health
Ida Pino, Gaia Maria Belloni, Valeria Barbera, Eugenio Solima, Davide Radice, Stefano Angioni, Saverio Arena, Valentino Bergamini, Massimo Candiani, Antonio Maiorana, Alberto Mattei, Ludovico Muzii, Luca Pagliardini, Maria Grazia Porpora, Valentino Remorgida, Renato Seracchioli, Paolo Vercellini, Fulvio Zullo, Errico Zupi, Michele Vignali
Summary: The mean diagnostic delay of symptomatic endometriosis in Italy is about 11 years, with patients under the age of 20 experiencing a delay of up to 4 years. There is no significant association between a delayed diagnosis and clinically relevant factors such as the severity of symptoms or the method of diagnosis.
EUROPEAN JOURNAL OF CONTRACEPTION AND REPRODUCTIVE HEALTH CARE
(2023)
Article
Obstetrics & Gynecology
C. Massarotti, G. Asinaro, M. G. Schiaffino, C. Ronzini, I. Vacca, M. Lambertini, P. Anserini, L. Del Mastro, A. Cagnacci
Summary: This study aimed to evaluate the efficacy of vaginal oxygen and hyaluronic acid on genito-urinary symptoms of breast cancer survivors. Treatment with vaginal oxygen and hyaluronic acid every 15 days improved genito-urinary symptoms of breast cancer patients. However, additional studies are needed to determine the optimal treatment interval, duration, and long-term benefits.
Review
Oncology
Maria G. Razeti, Davide Soldato, Luca Arecco, Alessia Levaggi, Silvia Puglisi, Cinzia Solinas, Elisa Agostinetto, Stefano Spinaci, Laura Lapuchesky, Carlo Genova, Claudia Massarotti, Matteo Lambertini
Summary: In patients with early breast cancer, the combination of different treatment strategies has shown significant improvements in survival outcomes. However, this comes at the cost of higher toxicity, highlighting the need for increased attention to survivorship-related issues like fertility preservation. Guidelines recommend oncofertility counseling for all reproductive-aged cancer patients, but implementation of these recommendations is suboptimal. In this review, the available evidence on oncofertility is summarized to guide healthcare providers in managing young women with breast cancer.
CLINICAL BREAST CANCER
(2023)
Editorial Material
Obstetrics & Gynecology
Sofia Makieva, Juan J. Fraire-Zamora, Mina Mincheva, Julia Uraji, Zoya E. Ali, Omar F. Ammar, George Liperis, Munevver Serdarogullari, Enrica Bianchi, Jonathan Pettitt, Karen Sermon, Siladitya Bhattacharya, Claudia Massarotti
HUMAN REPRODUCTION
(2023)
Article
Developmental Biology
Irene Gazzo, Claudia Massarotti, Sandra Chiodi, Simonetta Spinelli, Francesca Gualandi, Ubaldo Passamonti, Ezio Fulcheri, Emanuele Angelucci, Angelo Cagnacci
Summary: Hematopoietic stem cell transplantation (HSCT) is a treatment option for various blood diseases. With the increasing use of reproductive technologies, more patients who have undergone HSCT are conceiving, but the pathogenesis of obstetrical complications in these cases is not fully understood. This study presents data on two pregnancies in women who had undergone HSCT, showing that both pregnancies had intrauterine growth retardation due to defective implantation and placenta development, possibly caused by uterine vessel damage from irradiation. Further research is needed to understand the factors influencing placenta development post total body irradiation and HSCT for better pregnancy outcomes in this population.
Review
Obstetrics & Gynecology
Sarah Buckley, Kerstin Uvnas-Moberg, Zada Pajalic, Karolina Luegmair, Anette Ekstrom-Bergstrom, Anna Dencker, Claudia Massarotti, Alicja Kotlowska, Leonie Callaway, Sandra Morano, Ibone Olza, Claudia Meier Magistretti
Summary: Synthetic oxytocin increases maternal plasma oxytocin levels during labor, but does not affect newborn oxytocin levels. However, its use during labor can change uterine contraction patterns, potentially harming the fetus and increasing maternal pain and stress.
BMC PREGNANCY AND CHILDBIRTH
(2023)
Editorial Material
Cell Biology
Claudia Massarotti, Sofia Makieva, Sara Stigliani
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Letter
Oncology
C. Massarotti, S. Stigliani, I. Gazzo, M. Lambertini, P. Anserini
Article
Health Care Sciences & Services
Ambrogio P. Londero, Claudia Massarotti, Anjeza Xholli, Arrigo Fruscalzo, Angelo Cagnacci
Summary: This study reevaluated the association between assisted reproduction technologies (ART) and breech presentation, and found that both ART and other subfertility treatments (OST) were independent risk factors for breech at delivery. The prevalence of breech remained stable during the study period for both ART and OST.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Obstetrics & Gynecology
Sara Stigliani, Claudia Massarotti, Elena Maccarini, Fausta Sozzi, Simona Rebora, Paola Scaruffi, Paola Anserini
Summary: This study evaluated the use of telehealth in managing infertile couples. The results showed that couples who received telehealth had a shorter time to first treatment and a reduced drop-out rate compared to those who had face-to-face visits. Telehealth proved to be a useful tool in facilitating patient care at a fertility center.
MINERVA OBSTETRICS AND GYNECOLOGY
(2023)
Meeting Abstract
Obstetrics & Gynecology
S. Stigliani, A. Amaro, F. Reggiani, E. Maccarini, C. Massarotti, M. Lambertini, P. Anserini, P. Scaruffi
HUMAN REPRODUCTION
(2023)
Meeting Abstract
Obstetrics & Gynecology
C. Massarotti, S. Chiodi, S. Spinelli, S. Stigliani, E. Angelucci, P. Anserini
HUMAN REPRODUCTION
(2023)
Article
Andrology
Sara Stigliani, Claudia Massarotti, Simona Coco, Guglielmo Mantica, Nataniele Piol, Bruno Spina, Elena Maccarini, Monica Colombo, Paola Anserini, Paola Scaruffi
Summary: Severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) causes COVID-19, which has been spreading worldwide since December 2019. This study investigated the presence of SARS-CoV-2 mRNA in the prostatic tissue of an active infection patient and in the recovered prostate tissues of five patients. The study concluded that SARS-CoV-2 RNA was not detected in any of the prostate tissues tested, even during the acute phase of infection.
WORLD JOURNAL OF MENS HEALTH
(2023)
Article
Public, Environmental & Occupational Health
Sara Stigliani, Claudia Massarotti, Francesca Bovis, Elena Maccarini, Paola Anserini, Paola Scaruffi
Summary: The study findings indicate that COVID-19 disease does not have a negative effect on semen quality and male reproductive potential when semen samples are collected three months or more after infection.
FRONTIERS IN REPRODUCTIVE HEALTH
(2023)
Article
Obstetrics & Gynecology
Ambrogio P. Londero, Anjeza Xholli, Claudia Massarotti, Arrigo Fruscalzo, Angelo Cagnacci
Summary: This study aimed to assess the factors associated with the success and failure rate of external cephalic version (ECV) in breech fetuses. The results found factors such as nulliparity, maternal race, maternal age, female fetal sex, low fetal weight, and maternal anthropometric features to be correlated with ECV results.
ARCHIVES OF GYNECOLOGY AND OBSTETRICS
(2023)
