Article
Biochemistry & Molecular Biology
Giacomo Lazzarino, Romina Pallisco, Gabriele Bilotta, Ilaria Listorti, Renata Mangione, Miriam Wissam Saab, Giuseppe Caruso, Angela Maria Amorini, Maria Violetta Brundo, Giuseppe Lazzarino, Barbara Tavazzi, Pasquale Bilotta
Summary: The study analyzed metabolites in follicular fluid and identified 27 low molecular weight compounds with significantly different values in infertile females compared to control subjects. These compounds were used to calculate a Biomarker Score that effectively discriminated between control and infertile groups, indicating their potential as infertility biomarkers. Additionally, the Biomarker Score values correlated with various outcome measures of assisted reproduction techniques, suggesting a strong influence of follicular fluid metabolism on IVF success and embryonic development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Sweta Ravisankar, Kelsey E. Brooks, Melinda J. Murphy, Nash Redmayne, Junghyun Ryu, Jason M. Kinchen, Shawn L. Chavez, Jon D. Hennebold
Summary: The administration of gonadotropins during infertility treatment affects the development of ovarian follicles and the competency of oocytes. Metabolomics analysis identified unique metabolites in follicular fluid that were associated with different embryo classifications, with an increase in the cortisol to cortisone ratio observed in the blastocyst group. The glucocorticoid receptor NR3C1 plays a role in regulating follicular processes through paracrine signaling, but further studies are needed to understand its specific mechanisms.
SCIENTIFIC REPORTS
(2021)
Article
Environmental Sciences
Sueyoun Hwang, Robert B. Hood, Russ Hauser, Joel Schwartz, Francine Laden, Dean Jones, Donghai Liang, Audrey J. Gaskins
Summary: This study utilized metabolomics to investigate the relationship between air pollution exposure and oocyte quality, finding that higher NO2 exposure was associated with changes in multiple metabolites and biological pathways that may mediate the decrease in oocyte quality.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Agriculture, Dairy & Animal Science
Huaxing Zhao, Xiaohua He, Xianjun Zhang, Junsong Shi, Rong Zhou, Ranbiao Mai, Qiaoyun Su, Gengyuan Cai, Sixiu Huang, Zheng Xu, Zhenfang Wu, Zicong Li
Summary: The quality of in vitro matured oocytes is usually lower than that of in vivo matured oocytes, possibly due to the absence of some important signal regulators in vitro. In this study, we found that progesterone and androstenedione were upregulated in the follicular fluid (FF) during in vivo pig oocyte maturation. The supplementation of progesterone or androstenedione during in vitro maturation significantly improved the pig oocyte maturation rate and subsequent embryo developmental competence.
Review
Biochemistry & Molecular Biology
Ana Teresa Brinca, Ana Cristina Ramalhinho, Angela Sousa, Antonio Helio Oliani, Luiza Breitenfeld, Luis A. Passarinha, Eugenia Gallardo
Summary: PCOS is one of the main causes of anovulatory infertility and its etiology and pathophysiology are still unclear. PCOS patients undergoing assisted reproductive techniques may experience poor response, low oocyte quality, and ovarian hyperstimulation syndrome. Metabolomic analysis can improve the success rate of assisted reproductive technology.
Article
Obstetrics & Gynecology
Berna Aslan Cetin, Pelin Ocal, Tulay Irez, Ezel Uslu, Kubra Irmak, Suat Karatas
Summary: The study found a correlation between sialic acid levels in follicular fluid and oocyte quality during in vitro fertilization (IVF), but not with clinical parameters. Sialic acid levels in the follicular fluid may play a role in normal oocyte maturation or act as a compensatory mechanism against reactive oxygen species during the maturation process.
REPRODUCTIVE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Giedre Skliute, Brigita Vaigauskaite-Mazeikiene, Algirdas Kaupinis, Mindaugas Valius, Edita Kazenaite, Ruta Navakauskiene
Summary: The prevalence of infertility is increasing due to the rising age of first-time parents. Assisted reproductive technology procedures have drawn attention to the effects of follicle-stimulating hormone (FSH) on follicular fluid (FF) stromal cells. This study found that FSH had no impact on FF stromal cell morphology but reduced the proliferation rate. Other findings include the downregulation of PTGS2 in FF sediment cells of patients who did not conceive and differential effects of FSH on follicle development and FSH response-related genes depending on exposure length.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Reproductive Biology
Veronique Viardot-Foucault, Jieliang Zhou, Dexi Bi, Yoshihiko Takinami, Jerry. K. Y. Chan, Yie Hou Lee
Summary: This study investigated the effects of DHEA supplementation on the follicular fluid metabolome and cytokine profile in poor ovarian responders (POR) undergoing IVF. The results showed that DHEA supplementation altered the metabolome and cytokine profile, suggesting potential for individualized supplementation.
JOURNAL OF OVARIAN RESEARCH
(2023)
Article
Endocrinology & Metabolism
Ziyu Guo, Jihong Yang, Guangping Yang, Ting Feng, Xinyue Zhang, Yao Chen, Ruizhi Feng, Yun Qian
Summary: The study found that higher levels of NAM in large follicles are associated with larger follicle development and higher rates of oocyte maturation and fertilization. Adding 5mM NAM during in vitro maturation process can improve mice oocyte quality and reduce damage caused by oxidative stress.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Orianne Serri, Magalie Boguenet, Juan Manuel Chao de la Barca, Pierre-Emmanuel Bouet, Hady El Hachem, Odile Blanchet, Pascal Reynier, Pascale May-Panloup
Summary: Male infertility has been on the rise in the past decade, and the pathophysiologic mechanisms behind extreme oligospermia (EO) are not fully understood. In this metabolomics study, we identified metabolites associated with EO and found clear evidence of epididymal dysfunction in cases of testicular damage. This study highlights the complexity of metabolic dysfunction in male infertility and underscores the importance of metabolomics in understanding this condition.
Article
Biochemistry & Molecular Biology
Giulia Collodel, Laura Gambera, Anita Stendardi, Fabiola Nerucci, Cinzia Signorini, Caterina Pisani, Marzia Marcheselli, Francesca Letizia Vellucci, Silvana Enrica Pizzasegale, Lucia Micheli, Elena Moretti
Summary: This study investigated the changes and relationships of various molecules in the follicular fluid (FF) of infertile women with different reproductive conditions. It found that some biochemical components, hormones, antioxidant enzymes, F-2-Isoprostanes (F-2-IsoPs), and resolvin (Rv) D1 in the FF are associated with follicular growth, oocyte maturation, and female infertility. These findings highlight the importance of personalized therapeutic approaches and optimized assisted reproductive techniques (ART) outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Obstetrics & Gynecology
Anke Bullach, Tom Trapphoff, Sebastian Zuehlke, Michael Spiteller, Stefan Dieterle
Summary: This study found that a variety of endocrine-disrupting chemicals were present in human follicular fluid, with nonylphenol and Mirex being the most concentrated. These chemicals led to significantly reduced maturation and fertilization rates, but did not have a significant impact on the clinical pregnancy rate. The source of clothing and textiles may affect the accumulation of these chemicals in follicular fluid.
REPRODUCTIVE SCIENCES
(2021)
Article
Orthopedics
A. K. Hahn, A. Batushansky, R. A. Rawle, E. B. Prado Lopes, R. K. June, T. M. Griffin
Summary: High-fat diet significantly affects joint structure, while voluntary exercise has minimal impact. Diet-induced changes in synovial fluid metabolites are the most distinct. Diet and activity uniquely alter synovial fluid metabolites associated with amino acids, lipids, and steroids.
OSTEOARTHRITIS AND CARTILAGE
(2021)
Article
Biochemistry & Molecular Biology
Natalia Lomova, Natalia Dolgushina, Alisa Tokareva, Vitaly Chagovets, Natalia Starodubtseva, Ilya Kulikov, Gennady Sukhikh, Vladimir Frankevich
Summary: This study aimed to investigate the lipid profile of follicular fluid in COVID-19 patients and evaluate its impact on the outcomes of in vitro fertilization (IVF) procedures, in order to identify potential biomarkers for prognosis. Mass spectrometry was used to analyze follicular fluid samples from 237 patients, revealing significant changes in several lipids. The results suggest that lipid metabolism in follicular fluid may be associated with pregnancy occurrence and outcomes after severe COVID-19.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Yun Huang, Mixue Tu, Yuli Qian, Junyan Ma, Lifen Chen, Yifeng Liu, Yiqing Wu, Kai Chen, Juan Liu, Yanyun Ying, Yao Chen, Yinghui Ye, Lanfeng Xing, Fang Zhang, Yanjun Hu, Runjv Zhang, Ye Chun Ruan, Dan Zhang
Summary: Female fertility declines with age, and the metabolites in follicular fluids are closely related to oocyte quality. This study analyzed the metabolomic profile of follicular fluids from women of different ages and identified metabolites associated with oocyte quality and the outcomes of assisted reproductive technology (ART). These metabolites may serve as potential biomarkers for oocyte maturation and ART outcomes.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biology
Jin-Ran Chen, Haijun Zhao, Umesh D. Wankhade, Sree V. Chintapalli, Can Li, Dongzheng Gai, Kartik Shankar, Fenghuang Zhan, Oxana P. Lazarenko
Summary: This study shows that GPR109A deletion in mice leads to higher bone mass and strength, decreased osteoclast numbers, and suppressed bone resorption markers in bone marrow. Additionally, GPR109A deletion inhibits Wnt/beta-catenin signaling in osteoclast precursors, leading to reduced osteoclast differentiation and activity. Hippuric acid (HA) inhibits bone resorption and increases bone mass in wild type mice, but not in GPR109A knockout mice, indicating a role of GPR109A in mediating the effects of HA on bone resorption during skeletal development.
COMMUNICATIONS BIOLOGY
(2021)
Article
Food Science & Technology
Chrissa Petersen, Divya Bharat, Umesh D. Wankhade, Ji-Seok Kim, Brett Ronald Cutler, Christopher Denetso, Samira Gholami, Samantha Nelson, Jessica Bigley, Aspen Johnson, Sree Chintapalli, Brian D. Piccolo, Adhini Kuppuswamy Satheesh Babu, Henry A. Paz, Kartik Shankar, J. David Symons, Pon Velayutham Anandh Babu
Summary: This study investigates the effects of dietary blueberries on vascular complications and gut microbiome in diabetic mice. The results show that blueberry supplementation can alleviate vascular inflammation, improve arterial endothelial function, and support the growth of commensal microbes.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Endocrinology & Metabolism
Catherine C. Cohen, Wei Perng, Traci A. Bekelman, Brandy M. Ringham, Ann Scherzinger, Kartik Shankar, Dana Dabelea
Summary: This study aimed to examine the association between nutrient intakes in childhood and abdominal and hepatic fat depots in adolescence. It found that fat intake in childhood was associated with abdominal subcutaneous adipose tissue, starch intake was associated with hepatic fat, and animal protein intake was associated with visceral adipose tissue in boys.
Article
Pediatrics
Catherine C. Cohen, Ellen C. Francis, Wei Perng, Katherine A. Sauder, Ann Scherzinger, Shikha S. Sundaram, Kartik Shankar, Dana Dabelea
Summary: This study found an association between maternal lipid concentrations, especially in early pregnancy, and higher offspring hepatic fat. Adjusting for potential confounders or mediators did not affect the associations.
Article
Endocrinology & Metabolism
Begum Aydogan Mathyk, Brian D. Piccolo, Fernanda Alvarado, Kartik Shankar, Perrie O'Tierney-Ginn
Summary: Maternal obesity is associated with neonatal adiposity and cord blood metabolites, but these associations differ between offspring of obese and normal-weight women, with different fatty acid profiles observed in the cord blood of obese women's offspring.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2022)
Article
Nutrition & Dietetics
Martin J. J. Ronis, Horacio Gomez-Acevedo, Kartik Shankar, Leah Hennings, Neha Sharma, Michael L. Blackburn, Isabelle Miousse, Harry Dawson, Celine Chen, Kelly E. Mercer, Thomas M. Badger
Summary: This study found that soy infant formula does not have estrogenic effects or significantly alter male reproductive development in newborn male piglets, compared to sow milk and milk formula.
Article
Pediatrics
Stephanie P. Gilley, Meghan L. Ruebel, Clark Sims, Ying Zhong, Donald Turner, Renny S. Lan, Lindsay M. Pack, Brian D. Piccolo, Sree Chintapalli, Ann Abraham, Lars Bode, Aline Andres, Kartik Shankar
Summary: Maternal obesity is associated with changes in infant fecal microbiome, short-chain fatty acids (SCFA), and human milk oligosaccharides (HMO). Offspring of women with obesity have lower abundance of SCFA-producing bacteria and lower levels of faecal butyric acid. Overall microbial richness may aid in prediction of elevated adiposity in later infancy.
Article
Endocrinology & Metabolism
Zaramasina L. Clark, Meghan L. Ruebel, Peter Z. Schall, Kaitlin R. Karl, James J. Ireland, Keith E. Latham
Summary: High doses of follicle-stimulating hormone (FSH) during ovarian stimulation can negatively affect the function and quality of ovulatory follicles and oocytes. This study investigated the mechanisms behind this phenomenon using a small ovarian reserve heifer model. The researchers found that excessive FSH doses led to phenotypic heterogeneity of ovulatory size follicles and progressive changes in the transcriptomes of granulosa cells, cumulus cells, and oocytes. These changes were associated with disruptions in key pathways and could explain the observed reduction in estradiol production, increased progesterone and oxytocin production, and diminished ovulation rates. The study also predicted deficiencies in oocyte survival, stress response, and fertilization, suggesting further negative impacts on oocyte health and assisted reproductive technology outcomes.
Article
Biochemistry & Molecular Biology
Jin-Ran Chen, Perry C. Caviness, Haijun Zhao, Beau Belcher, Umesh D. Wankhade, Kartik Shankar, Michael L. Blackburn, Oxana P. Lazarenko
Summary: Studies have shown that maternal chronic poor-quality diet, especially a high fat diet, is associated with reduced bone density and childhood fractures in offspring. The research findings indicate that maternal high fat diet changes histone methylation and acetylation epigenetic marks, which regulate the expression of genes controlling osteoblastogenesis.
Article
Agriculture, Dairy & Animal Science
Meghan L. Ruebel, Lilian Rigatto Martins, Peter Z. Schall, J. Richard Pursley, Keith E. Latham
Summary: The objective of this study was to investigate the effect of early lactation body condition loss on serum lipids and its influence on oocyte and cumulus cell transcriptomes in multiparous dairy cows. The study found that cows with body condition loss had increased levels of certain fatty acids in their serum and significant changes in gene expression in oocytes and cumulus cells compared to cows with maintained or gained body condition. These findings provide new insights into the mechanisms of reduced fertility in dairy cows.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Microbiology
Minghua Tang, Nicholas E. Weaver, Daniel N. Frank, Diana Ir, Charles E. Robertson, Jennifer F. Kemp, Jamie Westcott, Kartik Shankar, Ana L. Garces, Lester Figueroa, Antoinette K. Tshefu, Adrien L. Lokangaka, Shivaprasad S. Goudar, Manjunath Somannavar, Sumera Aziz, Sarah Saleem, Elizabeth M. McClure, K. Michael Hambidge, Audrey E. Hendricks, Nancy F. Krebs
Summary: The objective of this study was to characterize the changes in gut microbiota during pregnancy and to determine the effects of nutritional intervention on gut microbiota in women from sub-Saharan Africa, South Asia, and Central America. The results showed that the composition of gut microbial community changed significantly during pregnancy, with a decrease in alpha-diversity. Nutritional intervention did not have a significant impact on inflammatory biomarkers.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Catherine C. Cohen, Dana Dabelea, Gregory Michelotti, Lu Tang, Kartik Shankar, Michael Goran, Wei Perng
Summary: This study aimed to assess the intermediary metabolic alterations between sugar-sweetened beverage intake and cardiometabolic risk factors in youth. The researchers identified a panel of lipid metabolites that may serve as biomarkers linking sugar-sweetened beverage intake to dyslipidemia risk in youth.
Article
Physiology
Harrison D. Stierwalt, E. Matthew Morris, Adrianna Maurer, Udayan Apte, Kathryn Phillips, Tiangang Li, Grace M. E. Meers, Lauren G. Koch, Steven L. Britton, Greg Graf, R. Scott Rector, Kelly Mercer, Kartik Shankar, John P. Thyfault
Summary: The study found that high aerobic capacity and exercise are associated with upregulation of bile acid synthesis and greater fecal excretion of cholesterol and bile acid, which may contribute to the protection against hepatic steatosis in rodents.
PHYSIOLOGICAL REPORTS
(2022)
Article
Nutrition & Dietetics
Catherine C. Cohen, Kylie K. Harrall, Stephanie P. Gilley, Wei Perng, Katherine A. Sauder, Ann Scherzinger, Kartik Shankar, Shikha S. Sundaram, Deborah H. Glueck, Dana Dabelea
Summary: This study found that children with higher hepatic fat in early childhood had smaller birth weight and faster adiposity accretion in the first 5 years. This suggests that promoting healthy body composition early in life may be critical for preventing pediatric NAFLD.
AMERICAN JOURNAL OF CLINICAL NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Henry A. Paz, Anna-Claire Pilkington, Ying Zhong, Sree Chintapalli, James Sikes, Renny S. Lan, Kartik Shankar, Umesh D. Wankhade
Summary: The gut microbiota plays a critical role in energy homeostasis and its dysbiosis is associated with obesity. Maternal high-fat diet and beta-adrenergic stimuli independently alter the gut microbiota, but their combined regulation is not clear. This study investigated the combined effect of these factors on offspring microbiota and found that maternal high-fat diet altered the offspring's response to beta-adrenergic stimuli, as well as microbial composition and function. Additionally, exposure to different temperatures also affected bacterial function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)