Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 10, Issue 51, Pages 44240-44246Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b15629
Keywords
DNA nanotechnology; DNA origami; DNA nanostructure; protein crystallization; DNA-protein interaction
Funding
- National Natural Science Foundation of China [21474059, 21372258]
- Royal Academy of Engineering Research Exchange China and India (RECI) programme [1314RECI047]
- EPSRC [EP/N015916/1]
- EPSRC [EP/N015916/1] Funding Source: UKRI
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This study reports the first experimental evidence of DNA origami as a seed resulting in the increase in probability of protein crystallization. Using the DNA origami constructed from long single-stranded M13 DNA scaffolds folded with short single-stranded DNA staples, it was found that the addition of the DNA origami in concentrations of 2-6 nM to mixtures of a well-characterized protein (catalase) solution (1.0-7.0 mg/mL) resulted in a higher proportion of mixtures with successful crystallization, up to 11x greater. The improvement in crystallization is evident particularly for mixtures with low concentrations of catalase (<5 mg/mL). DNA origami in different conformations of a flat rectangular sheet and a tubular hollow cylinder were examined. Both conformations improved the crystallization as compared to control experiments without M13 DNA or nonfolded M13 DNA but exhibited little difference in the extent of protein crystallization improvement. This work confirms the predictions of the potential use of DNA origami to promote protein crystallization, with potential application to systems with limited protein availability or difficulty in crystallization.
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