Article
Biochemistry & Molecular Biology
Xingang Li, Yuanting Pang, Yiwang Zhong, Zhandong Cai, Qibin Ma, Ke Wen, Hai Nian
Summary: In this study, a tau-like glutathione transferase family gene named GmGSTU23 was identified in soybean, which showed a concentration-time-specific expression pattern in response to salt stress. Transgenic lines with GmGSTU23 displayed enhanced salt tolerance, root length, and fresh weight compared to the wild type. Changes in glutathione pools and associated enzyme activity were observed, indicating that GmGSTU23 enhances the activity of glutathione transferase to scavenge reactive oxygen species and glutathione, thereby conferring enhanced tolerance to salt stress in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xiangkun Meng, Zhaolu Wu, Chengyun Jiang, Daojie Guan, Nan Zhang, Heng Jiang, Qinwen Shen, Kun Qian, Jianjun Wang
Summary: This study identified GSTs genes from the rice stem borer Chilo suppressalis and analyzed their classification, sequence characteristics, and genome structures. The research found that specific GSTs genes were significantly upregulated in C. suppressalis after treatment with abamectin, indicating their association with abamectin detoxification. These findings provide valuable information on the role of GSTs in abamectin detoxification in C. suppressalis and other insects.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Qinghai Cao, Wuyun Lv, Hong Jiang, Xueling Chen, Xinchao Wang, Yuchun Wang
Summary: In this study, a comprehensive identification and analysis of GSTs in tea plant were conducted, and their important roles in plant growth, development, and stress responses were revealed. The study found that CsGSTs were unevenly distributed across the tea plant genome and tandem duplication was the main driving force for their expansion. Furthermore, CsGSTs were found to respond to multiple stresses and exhibit different expression patterns in different tissues. CsGSTU19, localized in the nucleus and cell membrane, was proven to be involved in tea plant defense against temperature stresses and Co. camelliae infection.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Multidisciplinary Sciences
Tahmina Islam, M. K. Reddy
Summary: Non-essential toxic heavy metals like cadmium (Cd2+) have adverse effects on the growth and development of plants and can potentially harm human health. This study focused on the role of a peroxidase gene family member, PgGPx, in rice under Cd2+ stress. The overexpression of PgGPx enhanced Cd2+ tolerance in transgenic rice plants and improved oxidative stress response by increasing the activity of antioxidant enzymes. These findings provide insights for developing strategies to engineer Cd2+ stress tolerance in economically important crop plants.
Article
Biochemistry & Molecular Biology
Youfu Lin, Qi Xiao, Qianwen Hao, Zijin Qian, Xuexia Li, Peng Li, Hong Li, Lian Chen
Summary: This study investigated the role of Glutathione S-transferases (GSTs) in cold acclimation and cold stress-response mechanisms in the invasive snail P. canaliculata. A total of 30 PcGSTs were classified into seven classes and their expression patterns in different tissues were examined. RNA-seq analysis and qRT-PCR experiments revealed significant changes in expression levels of certain PcGST genes during cold acclimation, providing insights into the mechanisms underlying cold stress responses.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Genetics & Heredity
Xue Song Liu, He Li, Sheng Jun Feng, Zhi Min Yang
Summary: This study reveals the role of a rice Zeta family glutathione-S-transferase (OsGSTZ4) gene in Cd detoxification and its interaction with CHH hypermethylation and small interfering RNA (siRNA) in Cd response. These findings contribute to our understanding of the epigenetic mechanism of gene regulation for rice adaptation to environmental stress.
Article
Plant Sciences
Linlin Wang, Hongbo Fu, Juan Zhao, Jiagang Wang, Shuqi Dong, Xiangyang Yuan, Xiaorui Li, Mingxun Chen
Summary: This study conducted genome-wide identification and expression characteristics analysis of the GST gene family in foxtail millet. The results showed the presence of 73 GST genes (SiGSTs) divided into seven classes in the foxtail millet genome. The study provides a theoretical basis for identifying foxtail millet GST family information and improving their responses to different stresses.
Article
Environmental Sciences
Ambika Pandey, Lin-Bo Wu, Varunseelan Murugaiyan, Gabriel Schaaf, Jauhar Ali, Michael Frei
Summary: Contamination of paddy soils with arsenic can have toxic effects on rice plants and increase arsenic accumulation in grains. The uptake and detoxification of different arsenic species in rice is not fully understood. This study investigated the toxic effects of arsenite and arsenate on rice plants and the role of glutathione S-transferase in detoxifying arsenic. The results showed that arsenite was more detrimental to plant growth and lipid peroxidation than arsenate. Overexpression of glutathione S-transferase led to improved plant growth and lower lipid peroxidation under acute arsenite stress. Overall, arsenite was found to be more toxic to plants than arsenate and glutathione S-transferase has differential effects on plant reactions and tolerance to different arsenic species.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
Y. Yang, J. Li, H. Li, Y. Ding, W. Wu, R. Qin, J. Ni, R. Xu, P. Wei, J. Yang
Summary: Two newly identified GST genes, OsGSTU5 and OsGSTU37, play a role in the regulatory mechanism of cadmium tolerance in rice by enhancing the activity of antioxidant enzymes and glutathione reductase.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2023)
Article
Horticulture
Fei Ding, Chuang Wang, Shuoxin Zhang, Meiling Wang
Summary: This study reveals the critical role of a JA-responsive glutathione S-transferase gene (SlGSTU24) in alleviating cold stress in tomato. SlGSTU24 is expressed in all tissues and is responsive to both JA and cold stress. Upregulation of SlGSTU24 attenuates cold-induced oxidative stress, while its expression is dependent on the crucial regulator of JA signaling pathway, SlMYC2. These findings uncover a crucial module MYC2-SlGSTU24 that maintains ROS homeostasis in tomato under cold conditions.
SCIENTIA HORTICULTURAE
(2022)
Article
Biochemistry & Molecular Biology
Warin Rangubpit, Eukote Suwan, Danai Sangthong, Kannika Wongpanit, Roger W. Stich, Prapasiri Pongprayoon, Sathaporn Jittapalapong
Summary: In this study, molecular dynamics simulations were conducted to investigate the binding of GTX and GSH to RmGST. It was found that GSH binds tighter and sits rigidly inside the G-site, while GTX occupies both active sites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Bo Li, Xiangzhan Zhang, Ruiwei Duan, Chunhong Han, Jian Yang, Long Wang, Suke Wang, Yanli Su, Lei Wang, Yongfei Dong, Huabai Xue
Summary: In this study, a total of 57 PcGST genes involved in anthocyanin accumulation in pear peels were identified through genomic analysis. Glutamic acid was found to significantly improve anthocyanin accumulation, with PcGST57 being the most significantly induced gene. Further experiments showed that overexpression of PcGST57 increased anthocyanin accumulation, while silencing of PcGST57 reduced anthocyanin content. These findings provide candidate genes for red pear breeding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Environmental
Madhu Tiwari, Maria Kidwai, Prasanna Dutta, Shiv Narayan, Neelam Gautam, Khushboo Chawda, Pramod Arvind Shirke, Arun Kumar Mishra, Debasis Chakrabarty
Summary: Arsenic is a hazardous metalloid that hampers physiological activities in rice. In this study, the role of OsGSTU5 in arsenic tolerance was investigated. OsGSTU5 was found to enhance arsenic tolerance by regulating antioxidant activities and participating in arsenic chelation and transport processes.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Environmental Sciences
Ahmed A. A. Aioub, Ahmed S. S. Hashem, Ahmed H. H. El-Sappah, Amged El-Harairy, Amira A. A. Abdel-Hady, Laila A. A. Al-Shuraym, Samy Sayed, Qiulan Huang, Sarah I. Z. Abdel-Wahab
Summary: This study identified and characterized GST genes in the fall armyworm S. frugiperda and evaluated the toxicity of emamectin benzoate (EBZ) and chlorantraniliprole (CHP) on the larvae. A total of 31 GST genes were identified, and SfGSTe10 and SfGSTe13 showed the highest expression after EBZ and CHP treatments. Molecular docking analysis revealed high binding affinity between EBZ and CHP with SfGSTe10 and SfGSTe13. These findings provide important insights into the detoxification processes of S. frugiperda regarding EBZ and CHP.
Article
Plant Sciences
Liwen Cui, Yu Chen, Jun Liu, Qiang Zhang, Lei Xu, Zhimin Yang
Summary: Cadmium (Cd) is a highly toxic metal that poses serious threats to both plant growth and human health. Hence, it is crucial to explore simple, practical, and eco-friendly methods to mitigate its toxicity. Previous research has mainly focused on the physiological and biochemical aspects of zinc sulfate application for enhancing plant tolerance to Cd, with insufficient investigation into the underlying molecular mechanisms. In this study, high and low concentrations of zinc sulfate were sprayed on seashore paspalum plants under Cd stress, and transcriptome sequencing and physiological indicators were employed to elucidate the mechanisms of Cd tolerance.
