Article
Food Science & Technology
Babak Pakbin, Wolfram Manuel Bruck, Samaneh Allahyari, John W. A. Rossen, Razzagh Mahmoudi
Summary: This study investigated the prevalence, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of C. sakazakii strains isolated from powdered infant formula milk (PIFM) samples collected in Tehran, Iran. The results showed a high prevalence rate of C. sakazakii in PIFM samples, with high resistance to multiple antibiotics and a diverse genetic profile. The study highlights the need for novel microbial surveillance systems to control the contamination of this foodborne pathogen in infant foods.
Article
Agriculture, Dairy & Animal Science
Ju Liu, Guoyang Xie, Qin Xiong, Dan Mu, Hengyi Xu
Summary: A simple and sensitive fluorescent turn-off aptasensor was developed for detecting viable Cronobacter sakazakii in powdered infant formula. The sensor showed good performance and specificity in detecting the pathogen within 3 hours in both pure culture and formula samples.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Nutrition & Dietetics
Weiming Wang, Meng Geng, Caixia Zhu, Lei Huang, Yue Zhang, Tengxun Zhang, Chongjie Zhao, Tongcun Zhang, Xinjun Du, Nan Wang
Summary: The study revealed that the novel probiotic Ligilactobacillus salivarius YL20 could protect against C. sakazakii-induced intestinal injury by promoting epithelial cell proliferation, rescuing budding-impaired organoids, and enhancing intestinal barrier function in vitro and in vivo models.
Article
Food Science & Technology
Ondrej Holy, Julio Parra-Flores, Sarah Lepuschitz, Maria Paula Alarcon-Lavin, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Jetsi Mancilla-Rojano, Werner Ruppitsch, Stephen Forsythe
Summary: This study investigated the putative virulence factors of six Cronobacter sakazakii strains isolated from powdered milk, identifying different sequence types and resistance genes. Some strains demonstrated high adherence and invasion abilities, while the cpa gene was not detected. Various virulence and resistance genes, as well as plasmids, were identified through whole-genome sequencing. Additionally, CRISPR matrices were observed in all strains, enhancing decision-making for health authorities to protect public health.
Article
Food Science & Technology
Yuan-song Zhang, Xuechao Xu, Juanli Yang, Mengyuan Tan, Wenyuan Zhou, Lu Gao, Zhen-quan Yang
Summary: In this study, a chromogenic system based on phage-directed immobilized nanozyme was developed for colorimetric measurement of Cronobacter sakazakii in powdered infant formula (PIF). The method showed high specificity and low detection limit, successfully detecting C. sakazakii and achieving good recovery rates.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Immunology
Margaret G. Taylor, Megan H. Amerson-Brown, Kristina Hulten, Lindsay H. Cameron, Galit Holzmann-Pazgal, Morven S. Edwards, Catherine E. Foster
Summary: This study reports two cases of infants hospitalized with Cronobacter sakazakii meningitis, both of whom had exposure to powdered infant formula at home. Early advanced brain imaging is recommended, and reporting to public health officials may help identify outbreaks.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2021)
Article
Food Science & Technology
Zhanwen Zheng, Yawen Xie, Sheng Ma, Junhong Tu, Jiahui Li, Sen Liang, Yunfeng Xu, Chao Shi
Summary: The study demonstrated that 405-nm LED light effectively inactivated C. sakazakii in PIF, reducing its tolerance to various environmental stressors. LED illumination also downregulated the transcription levels of tolerance-associated genes and caused damage to bacterial cell membranes. This suggests the potential of 405-nm LED technology in preventing and controlling pathogens in food processing and storage environments.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Food Science & Technology
Geng Zou, Libere Ndayishimiye, Lingxiang Xin, Manshan Cai, Longjian Zhang, Jie Li, Zhiyong Song, Renwei Wu, Yang Zhou, Yuanguo Shi, Yingwang Ye, Rui Zhou, Jinquan Li
Summary: In this study, a phage termed LPCS28 with a broad lysis spectrum against C. sakazakii was isolated from environmental water samples. LPCS28 showed high thermal stability, with no significant decrease in titer after heating at 60 degrees C for one hour. Upon thermal processing at 63 degrees C for 30 min, LPCS28 effectively inhibited C. sakazakii in reconstituted powdered infant formula and liquid milk, reducing its concentration to below the limit of detection within 9 h at 37 degrees C. Phylogenetic analysis classified LPCS28 as a new genus, Nanhuvirus, within the family Straboviridae. These results highlight the potential of LPCS28 as a biological control agent against pathogenic C. sakazakii in the dairy industry.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Lihan Wang, Xinyi Pang, Jiayuan Zhao, Haonan Jin, Xinyan Yang, Shiqian Fu, Shasha Cheng, Hongxuan Li, Chao Miao, Chaoxin Man, Yujun Jiang
Summary: JK004 is a novel virulent phage isolated from the native environment of C. sakazakii, known for its tolerance to heat, pH, and osmotic-stress. It shows excellent antibacterial activity against C. sakazakii and biofilms, without containing any known virulence, transduction, or antibiotic resistance genes, indicating its safety for practical applications in food production. The study suggests the potential of JK004 as an effective strategy for controlling C. sakazakii.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Yanpeng Yang, Sheng Ma, Kailun Guo, Du Guo, Jiahui Li, Muxue Wang, Yutang Wang, Chunling Zhang, Xiaodong Xia, Chao Shi
Summary: This study found that combined LED illumination and citral treatment can effectively reduce the abundance of C. sakazakii in reconstituted powdered infant formula. The combination treatment also destroys cellular morphology and membrane integrity, and induces lipid peroxidation. Neither LED illumination nor citral treatment results in DNA breakdown in C. sakazakii.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Immunology
Haiyan Zeng, Chengsi Li, Jumei Zhang, Bingshao Liang, Hanjie Mei, Qingping Wu
Summary: We report a case of fatal neonatal necrotizing enterocolitis in China caused by a specific profile of Cronobacter sakazakii. Phylodynamic analysis revealed that the strain originated from a widespread and antimicrobial drug-sensitive sublineage of CRISPR. Enhanced surveillance and pathogenesis research on this organism are needed.
EMERGING INFECTIOUS DISEASES
(2023)
Article
Microbiology
Julio Parra-Flores, Ondrej Holy, Francisca Riffo, Sarah Lepuschitz, Eduard Maury-Sintjago, Alejandra Rodriguez-Fernandez, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Jetsi Mancilla-Rojano, Miriam Troncoso, Guillermo Figueroa, Werner Ruppitsch, Stephen Forsythe
Summary: This study used whole genome sequencing and laboratory studies to characterize Cronobacter strains from contaminated products, revealing multiple antibiotic resistance and virulence genes, supporting the decision to recall contaminated powdered and dairy formulas from the Chilean market in 2017.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Hye Ri Kim, Myunghee Kim, Byoung Chan Kim
Summary: In this study, C. sakazakii-specific aptamers were isolated using a centrifugation-based partitioning method, which showed high affinity and specificity for the pathogen. Among the two aptamers isolated, SC25 displayed efficient detection of C. sakazakii in powdered infant formula with less cross-reactivity, potentially reducing overall testing time compared to traditional methods.
Article
Food Science & Technology
Shijue Gao, Chongzhen Sun, Hanlu Hong, Ravi Gooneratne, Anthony Mutukumira, Xiyang Wu
Summary: In this study, a novel detection method was developed to effectively differentiate viable from dead bacteria, particularly in the detection of Cronobacter sakazakii in food samples. The method demonstrated faster and more accurate results compared to traditional culture-based methods, making it suitable for detecting complex food samples.
Article
Pediatrics
Siyuan Jiang, Lan Zhang, Weili Yan, Shujuan Li, Junyan Han, Qi Zhou, Yi Yang, Shoo K. Lee, Yun Cao
Summary: High antibiotic use rate, prolonged antibiotic durations, and excessive use of broad-spectrum antibiotics were the main issues of antibiotic use in Chinese neonatal intensive care units, highlighting the need for targeted antimicrobial stewardship interventions in the future.
JOURNAL OF PEDIATRICS
(2021)