Article
Biochemistry & Molecular Biology
Priyavathi Sureshkumar, Robson Augusto Souza dos Santos, Natalia Alenina, Stefan Mergler, Michael Bader
Summary: The G protein-coupled receptor MAS is the receptor for the endogenous ligand Angiotensin (Ang)-(1-7). This paper demonstrates the importance of MAS signaling in developing novel therapeutics for cardiovascular diseases. Ang-(1-7) induces calcium influx in MAS-transfected HEK293 cells, which is dependent on plasma membrane Ca2+ channels, phospholipase C, and protein kinase C. The study also reveals that MAS utilizes non-selective transient receptor potential channels (TRPs) for calcium entry.
Article
Critical Care Medicine
Bruno Garcia, Fuhong Su, Francesca Manicone, Laurence Dewachter, Raphael Favory, Amina Khaldi, Alexander Moiroux-Sahroui, Anthony Moreau, Antoine Herpain, Jean-Louis Vincent, Jacques Creteur, Fabio Silvio Taccone, Filippo Annoni
Summary: The aim of this study was to investigate the effects of Ang-(1-7) infusion on the development and severity of septic shock. The results showed that early Ang-(1-7) infusion can prevent the development of septic shock, reduce norepinephrine requirements, limit the increase of interleukin-6 levels, and prevent renal dysfunction.
Article
Immunology
Zhongjie Wang, Wenhan Huang, Feifeng Ren, Lei Luo, Jun Zhou, Dongmei Huang, Mei Jiang, Huaan Du, Jinqi Fan, Lin Tang
Summary: The study demonstrated that Ang-(1-7) and AVE0991 can reduce inflammatory cytokine levels, inhibit joint inflammation and bone destruction, as well as alleviate myocardial interstitial fibrosis. Ang-(1-7) may mediate these effects through the Mas receptor.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Endocrinology & Metabolism
Yuanyuan Liu, Haomeng Hao, Tingting Lan, Rui Jia, Mingya Cao, Liang Zhou, Zhiming Zhao, Wensen Pan
Summary: The local Renin-Angiotensin System (RAS) plays a role in the female reproductive system, regulating follicle development and physiological functions of the placenta and uterus. Ang II and Ang-(1-7) have similar functions in this system and can influence the occurrence and development of diseases.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zenghua Qi, Tao Wang, Xiangmao Chen, Chun Kit Wong, Qianqian Ding, Heinrich Sauer, Zhi-Feng Chen, Cheng Long, Xiaoqiang Yao, Zongwei Cai, Suk Ying Tsang
Summary: This study revealed the presence of intracellular Angiotensin II (iAng II) and its regulation of the automaticity of early differentiating cardiomyocytes via the AT(2)R and ryanodine receptor pathways. This provides evidence for the role of iAng II in regulating ESC-CMs behavior and fate determination.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Cell Biology
Itamar Couto Guedes Jesus, Thassio Mesquita, Robson Augusto Souza Santos, Silvia Guatimosim
Summary: The renin-angiotensin system (RAS) plays a crucial role in various cardiovascular functions. Angiotensin II, as one of the key peptides in this system, contributes to heart failure in pathological conditions. However, another RAS peptide, angiotensin-(1-7), exhibits therapeutic effects in different types of cardiac diseases. Recently, alamandine and its receptor, MrgD, have been discovered as new components of RAS, and their physiological and pathological functions in the heart, especially in cardiomyocytes, have been extensively studied.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Ning-ning Wang, Huan-Hua Xu, Wei Zhou, Hong-xing Yang, Jia Wang, Zeng-chun Ma, Yue Gao
Summary: Aconitine improves mitochondrial function by activating Sirt3 to deacetylate CypD and inhibiting mPTP opening, thus alleviating energy metabolic dysfunction and rescuing mitochondrial function in cardiomyocytes.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Endocrinology & Metabolism
Nan-Nan Sha, Jia-Li Zhang, Christina Chui-Wa Poon, Wen-Xiong Li, Yue Li, Yi-Fei Wang, Wei Shi, Fu-Hui Lin, Wen-Ping Lin, Yong-Jun Wang, Yan Zhang
Summary: In this study, it was found that ANG II and ANG(1-7) had different effects on osteoblasts and osteoclasts under high-glucose conditions, playing different roles in the development of osteoporosis, with ANG(1-7) showing a protective effect against diabetes-induced osteoporosis.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Orthopedics
Ying Li, Jiao Song, Yangyang Jiang, Xue Yang, Li Cao, Chun Xiao, Suli Li, Birong Dong, Xiaoli Huang
Summary: The ACE2/Ang-(1-7) axis plays a protective role in skeletal muscle function and administering exogenous Ang-(1-7) can delay age-related decline in skeletal muscle function. Aged mice showed reduced skeletal muscle function and Ang-(1-7) expression levels compared to younger mice. ACE2KO mice had significantly lower skeletal muscle function and MEF2A protein expression than age-matched wild-type mice. After one month of Ang-(1-7) treatment, skeletal muscle function in aged ACE2KO mice improved, with similar MEF2A protein expression to the untreated group. Ang-(1-7) was shown to promote Glut4 expression in C2C12 cells.
BMC MUSCULOSKELETAL DISORDERS
(2021)
Article
Cell Biology
Ruili Dang, Mengqi Yang, Changmeng Cui, Changshui Wang, Wenyuan Zhang, Chunmei Geng, Wenxiu Han, Pei Jiang
Summary: Activation of ACE2/Ang(1-7)/MasR axis triggers the FOXO1-autophagy pathway, inducing antioxidant enzymes SOD and CAT, leading to neuroprotection against neuroinflammation.
Article
Medicine, Research & Experimental
Elshymaa A. Abdel-Hakeem, Sara Mohamed Naguib Abdel Hafez, Bothina A. Kamel, Heba A. Abdel-Hamid
Summary: The study demonstrated the potential protective effect of Angiotensin 1-7 in rhabdomyolysis-induced kidney injury in rats, possibly through modulation of multiple pathways.
Article
Peripheral Vascular Disease
Matthew A. Sparks, Fitra Rianto, Edward Diaz, Ritika Revoori, Thien Hoang, Lucas Bouknight, Johannes Stegbauer, Anuradha Vivekanandan-Giri, Phillip Ruiz, Subramaniam Pennathur, Dennis M. Abraham, Susan B. Gurley, Steven D. Crowley, Thomas M. Coffman
Summary: Deletion of AT(1A) receptors in cardiomyocytes plays a crucial role in regression of cardiac hypertrophy, independent of reducing pressure overload.
Review
Pharmacology & Pharmacy
Minela Aida Maranduca, Daniela Maria Tanase, Cristian Tudor Cozma, Nicoleta Dima, Andreea Clim, Alin Constantin Pinzariu, Dragomir Nicolae Serban, Ionela Lacramioara Serban
Summary: The COVID-19 pandemic has placed significant stress on the medical community. ACE2, a cellular receptor for the virus, has been identified as a crucial component in the renin-angiotensin-aldosterone system (RAAS) and is responsible for various processes in different organs. Infection with SARS-CoV-2 leads to downregulation of ACE2 and disrupts the balance between ACE and ACE2 in organs, resulting in observed effects of COVID-19 infection.
Article
Chemistry, Medicinal
Sana Khajeh Pour, Arina Ranjit, Emma L. Summerill, Ali Aghazadeh-Habashi
Summary: This study evaluated the anti-inflammatory effects of a novel and stable conjugate of Ang-(1-7) in a rat model of arthritis. The results showed that the conjugate reduced inflammation-related body weight loss and joint edema, and decreased nitric oxide levels. In addition, the conjugate modulated the components of the renin-angiotensin system and exhibited enhanced therapeutic effects.
Article
Biochemistry & Molecular Biology
Dominga Lapi, Maurizio Cammalleri, Massimo Dal Monte, Martina Di Maro, Mariarosaria Santillo, Anna Belfiore, Gilda Nasti, Simona Damiano, Rossella Trio, Martina Chiurazzi, Barbara De Conno, Nicola Serao, Paolo Mondola, Antonio Colantuoni, Bruna Guida
Summary: The study found that Ang II and Ang 1-7 trigger different mechanisms through different receptors to affect cerebral microvascular injury in rats. Ang II exacerbates damage, while Ang 1-7 protects the microcirculation and can be regulated by specific receptor antagonists and activators.
Article
Cardiac & Cardiovascular Systems
Mario Morais Silva, Fernando Pedro de Souza-Neto, Itamar Couto Guedes de Jesus, Gleisy Kelly Goncalves, Melissa de Carvalho Santuchi, Bruno de Lima Sanches, Thais Cristina de Alcantara-Leonidio, Marcos Barrouin Melo, Maria Aparecida Ribeiro Vieira, Silvia Guatimosim, Robson Augusto Souza Santos, Rafaela Fernandes da Silva
Summary: Alamandine, the newest identified component of the renin-angiotensin system, has protective effects in the cardiovascular system by preventing cardiac remodeling such as myocyte hypertrophy and fibrosis. The study demonstrates that alamandine can improve various aspects of cardiac remodeling induced by pressure overload.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Neurosciences
Matheus P. S. Magalhaes-Gomes, Wallace Camargos, Priscila A. C. Valadao, Rubens S. Garcias, Hermann A. Rodrigues, Jessica N. Andrade, Vanessa P. Teixeira, Ligia A. Naves, Walter L. G. Cavalcante, Marcia Gallaci, Silvia Guatimosim, Vania F. Prado, Marco A. M. Prado, Cristina Guatimosim
Summary: Increased VAChT expression leads to higher levels of ACh release, but may have detrimental effects on the structure and function of the neuromuscular system, including impaired synaptic vesicle cycle and structural alterations of NMJs.
Article
Biochemistry & Molecular Biology
Itamar C. G. Jesus, Flavia M. Araujo, Thassio Mesquita, Nilton N. S. Junior, Mario M. Silva, Henrique J. N. Morgan, Kaoma S. C. Silva, Cleide L. A. Silva, Alexander Birbrair, Flavio A. Amaral, Luiz C. Navegantes, Helio C. Salgado, Raphael E. Szawka, Maristela O. Poletini, Silvia Guatimosim
Summary: The study identified a link between NE/beta 2-AR signaling and Per1 oscillation via the PI3Ky-ERK1/2-CREB pathway, providing a new framework for understanding the physiological mechanism involved in resetting cardiac clock genes.
Article
Pharmacology & Pharmacy
Igor Maciel Souza-Silva, Cristiane Amorim de Paula, Lucas Bolais-Ramos, Anderson Kenedy Santos, Filipe Alex da Silva, Vivian Louise Soares de Oliveira, Isabella Domingos da Rocha, Maisa Mota Antunes, Lidia Pereira Barbosa Cordeiro, Vanessa Pereira Teixeira, Sergio Ricardo Aluotto Scalzo Junior, Adriana Campezatto Raabe, Pedro Pires Goulart Guimaraes, Flavio Almeida Amaral, Jarbas Magalhaes Resende, Marco Antonio Peliky Fontes, Gustavo Batista Menezes, Silvia Guatimosim, Robson Augusto Souza Santos, Thiago Verano-Braga
Summary: BK-(1-7) and BK-(1-5) are endogenous peptides that are produced in vivo and have biological activity. Unlike BK-(1-9), the NO production induced by BK-(1-7) and BK-(1-5) is not inhibited by specific receptors. Additionally, these peptides can cause vasorelaxation, reduce blood pressure, and have a smaller effect on nociceptive responses compared to BK-(1-9). They also do not increase vascular permeability.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Quiara L. Alves, Paola V. da Silva Santos, William A. Santos, Suiane C. Dantas Damasceno, Rafael L. C. de Jesus, Thamires Q. Froes, Marcelo S. Castilho, Andrezza M. Barbosa, Eneas R. de Moraes Gomes, Jader dos Santos Cruz, Darizy F. Silva
Summary: The study demonstrates the direct cardiac effects of carvacrol, including reduced contractility of cardiomyocytes and negative chronotropic effect. The study also highlights the involvement of TRPM channels in mediating these effects.
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
(2022)
Article
Geriatrics & Gerontology
W. L. Camargo, C. Kushmerick, E. K. R. Pinto, N. M. Souza, W. L. G. Cavalcante, F. P. Souza-Neto, S. Guatimosim, M. A. A. Prado, C. Guatimosim, L. A. Naves
Summary: A study on mice at the neuromuscular junction revealed that an increase in the amount of acetylcholine per vesicle induces two distinct age-dependent homeostatic mechanisms compensating excessive acetylcholine release, demonstrating different effects at different ages.
NEUROBIOLOGY OF AGING
(2022)
Article
Cell Biology
Diogo A. Guimaraes, Nayara S. S. Aquino, Cibele Rocha-Resende, Itamar C. G. Jesus, Mario Morais Silva, Sergio A. Scalzo, Roberta Cristelli Fonseca, Marina T. Durand, Vanessa Pereira, Geisa C. S. Tezini, Andre Oliveira, Vania F. Prado, Ivanita Stefanon, Helio C. Salgado, Marco Antonio Maximo Prado, Raphael E. Szawka, Silvia Guatimosim
Summary: It is well known that cholinergic hypofunction contributes to cardiac pathology. This study found that mice with cholinergic deficit showed an increase in cardiac norepinephrine (NE) levels, enhanced sympathetic activity, and changes in cellular signaling pathways. These results reveal a previously unrecognized role of cholinergic signaling in modulating cardiac NE.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Yuxuan Guo, Yangpo Cao, Blake D. Jardin, Xiaoran Zhang, Pingzhu Zhou, Silvia Guatimosim, Junsen Lin, Zhan Chen, Yueyang Zhang, Neil Mazumdar, Fujian Lu, Qing Ma, Yao-Wei Lu, Mingming Zhao, Da-Zhi Wang, Erdan Dong, William T. Pu
Summary: The aim of this study is to investigate the role of RYR2 in cardiomyocyte maturation. The researchers used genetic editing techniques to knockout RYR2 and found that its depletion led to structural and transcriptional defects in cardiomyocytes, along with activation of ER stress pathways. The defects in RYR2-depleted cardiomyocytes were partially rescued by an ER stress alleviator.
CARDIOVASCULAR RESEARCH
(2023)
Article
Nanoscience & Nanotechnology
Sergio Scalzo, Anderson K. Santos, Heloisa A. S. Ferreira, Pedro A. Costa, Pedro H. D. M. Prazeres, Natalia J. A. da Silva, Lays C. Guimaraes, Mario de Morais e Silva, Marco T. R. Rodrigues Alves, Celso T. R. Viana, Itamar C. G. Jesus, Alice P. Rodrigues, Alexander Birbrair, Anderson O. Lobo, Frederic Frezard, Michael J. Mitchell, Silvia Guatimosim, Pedro Pires Goulart Guimaraes
Summary: Gene therapy using lipid nanoparticles (LNPs) as delivery vectors has shown promising potential for cardiac regeneration and gene correction in inherited cardiomyopathies. In this study, a mini-library of engineered LNPs was developed for the efficient delivery of pDNA to cardiomyocytes. The lead LNP formulation achieved enhanced gene expression in vitro and in vivo, with negligible toxicity and without affecting cardiac cell function. These findings highlight LNPs as a promising approach to improve gene therapy for cardiovascular disease treatment.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Endocrinology & Metabolism
Cristina S. Fonseca, Nayara S. S. Aquino, Gleisy K. N. Goncalves, Lucas R. Drummond, Laisa T. M. Hipolito, Juneo F. Silva, Kaoma S. C. Silva, Patricia C. Henriques, Talita E. Domingues, Ana C. R. Lacerda, Silvia Guatimosim, Candido C. Coimbra, Raphael E. Szawka, Adelina M. Reis
Summary: This study reveals the association between increased central norepinephrine and postmenopausal hot flushes, as well as the role of ARC kisspeptin neurons in regulating this symptom.
JOURNAL OF NEUROENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Carolina Nobre Ribeiro Pontes, Sergio Scalzo, Itamar Couto Guedes Jesus, Erika Fernandes de Jesus, Allancer Divino de Carvalho Nunes, Michelle Mendanha Mendonca, Elizabeth Pereira Mendes, Diego Basile Colugnati, Carlos Henrique Xavier, Gustavo Rodrigues Pedrino, Silvia Guatimosim, Carlos Henrique Castro
Summary: This study showed that Angiotensin-(1-7) counter-regulates the effect of acetylcholine on cardiac contractility through the Mas receptor in a manner independent of arterial pressure and heart rate.
Review
Cell Biology
Itamar Couto Guedes Jesus, Thassio Mesquita, Robson Augusto Souza Santos, Silvia Guatimosim
Summary: The renin-angiotensin system (RAS) plays a crucial role in various cardiovascular functions. Angiotensin II, as one of the key peptides in this system, contributes to heart failure in pathological conditions. However, another RAS peptide, angiotensin-(1-7), exhibits therapeutic effects in different types of cardiac diseases. Recently, alamandine and its receptor, MrgD, have been discovered as new components of RAS, and their physiological and pathological functions in the heart, especially in cardiomyocytes, have been extensively studied.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Instruments & Instrumentation
Ana Luiza de Castro Santos, Natalia Jordana Alves da Silva, Celso Tarso Rodrigues Viana, Leticia Cristine Cardoso dos Santos, Gabriel Henrique Costa da Silva, Sergio Ricardo Aluotto Scalzo Junior, Pedro Augusto Carvalho Costa, Walison Nunes da Silva, Itamar Couto Guedes de Jesus, Alexander Birbrair, Mariana T. Q. de Magalhaes, Frederic Frezard, Silvia Guatimosim, Rebecca M. Haley, Michael J. Mitchell, Silvia Passos Andrade, Paula Peixoto Campos, Pedro Pires Goulart Guimaraes
Summary: This study investigated the effects of the Wnt inhibitor complex CD: LGK974 on reducing the inflammatory, fibrotic, and angiogenic processes caused by intraperitoneal implants. The results showed that CD: LGK974 significantly reduced immune cell infiltration, inflammatory cytokine release, collagen deposition, and expression of pro-fibrotic markers. It also decreased VEGF levels and inhibited blood vessel formation. These findings suggest that the use of the Wnt inhibitor complex may be a potential strategy to reduce peritoneal fibrosis and implant rejection.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Oncology
Felipe Henrique de Souza Silva, Adam Underwood, Camila Pereira Almeida, Thais Salviana Ribeiro, Elaine M. Souza-Fagundes, Almir S. Martins, Marcos Eliezeck, Silvia Guatimosim, Luciana O. Andrade, Luisa Rezende, Hipacia Werneck Gomes, Cleida Aparecida Oliveira, Raquel Cristina Rodrigues, Isabella Terra Borges, Geovanni Dantas Cassali, Enio Ferreira, Helen Lima Del Puerto
Summary: The study suggests that the SOX3 transcription factor can promote apoptosis in the breast cancer cell line MDA-MB-231, while its nuclear expression is downregulated in cases of invasive ductal carcinoma. Further research is needed to investigate its role as a tumor suppressor protein and its regulation of genes involved in apoptosis.
Article
Biochemical Research Methods
Sergio Scalzo, Carolina A. T. F. Mendonca, Christopher Kushmerick, Ubirajara Agero, Silvia Guatimosim
Summary: This protocol provides instructions for obtaining high-quality cellular contractility data from various types of heart muscle cells. The optimized steps for microscope and camera setup and cellular selection criteria ensure robust and reliable data. The use of CONTRACTIONWAVE software for analysis is also demonstrated.
