Article
Immunology
Koemchhoy Khim, Sao Puth, Kamalakannan Radhakrishnan, Tien Duc Nguyen, Youn Suhk Lee, Che-Hun Jung, Shee Eun Lee, Joon Haeng Rhee
Summary: Enzymatic deglycosylation restores the TLR5 stimulating functionality of eukaryotic-expressed Vibrio vulnificus flagellin B (eFlaB), suggesting that glycosylation interferes with eFlaB binding to TLR5. Site-directed mutagenesis of N-glycosylation residues restores TLR5 stimulation and adjuvanticity.
Article
Agriculture, Multidisciplinary
Zecai Zhang, Jiang Huang, Zhicheng Zhao, Xueying Yuan, Chuang Li, Siyu Liu, Yueqi Cui, Yu Liu, Yulong Zhou, Zhanbo Zhu
Summary: Phlorizin, extracted from apple tree, has been found to inhibit the replication of BVDV and improve the histopathological changes caused by the infection. It works by regulating immune-related molecules, promoting interferon levels, and suppressing the expression of inflammatory cytokines.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Shinechimeg Dima, Hsiao-Ting Huang, Ikki Watanabe, Yu-Hua Pan, Yin-Yin Lee, Wei-Jen Chang, Nai-Chia Teng
Summary: The study utilized epsilon-polylysine and calcium phosphate precipitation methods to induce antibacterial effects and dentin tubule occlusion. Epsilon-polylysine demonstrated significant inhibition levels against P. gingivalis, with certain concentrations achieving complete bacterial inhibition and high dentin tubule occlusion. Longitudinal analysis revealed deep hydroxyapatite-like crystal formations in dentinal tubules after incubation in artificial saliva.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Julia Gao, Yingchao Su, Yi-Xian Qin
Summary: This study compared the in vitro and in vivo degradation and biocompatibility behaviors of magnesium alloys with calcium phosphate coatings, showing that the CaP coating significantly improved biodegradation behavior and biocompatibility of the Mg alloy. In vitro tests were found to be a quick screening tool for biodegradation rate, with the in vivo degradation rate being slower than in vitro immersion degradation rate.
BIOACTIVE MATERIALS
(2021)
Article
Materials Science, Multidisciplinary
Chun-Sheng Shao, Liang-Jian Chen, Rui-Min Tang, Bo Zhang, Jiang-Jie Tang, Wei-Na Ma
Summary: The combination of biphasic calcium phosphate (BCP) with osteomimetic porous micro-structure obtained via freeze casting is believed to achieve excellent bone regeneration, while the effects of different ratios of HA and beta-TCP on the degradation and biological performance of the BCP scaffolds need to be studied. The BCP scaffolds with varying HA/beta-TCP ratios demonstrated similar microstructure, mechanical performance, and protein absorption capacity, with the HA70/beta-TCP30 ratio showing optimal degradation rate. The in vitro and in vivo experiments confirmed the biocompatibility and bone regeneration capacity of the porous osteomimetic BCP scaffold with a 30:70 HA/beta-TCP ratio.
Article
Agriculture, Dairy & Animal Science
Noemie Gloanec, Daniel Dory, Segolene Quesne, Veronique Beven, Typhaine Poezevara, Michel Amelot, Marianne Chemaly, Muriel Guyard-Nicodeme
Summary: This study aimed to analyze the immune responses developed in broilers against Campylobacter and identify important immune parameters for designing new vaccine strategies. By comparing the immune responses in vaccinated and control groups, it was found that although specific anti-flagellin IgY levels and AvBD12 gene expression increased in the vaccinated group, there were no significant differences in specific anti-flagellin IgA antibody levels in bile and the relative expression of other cytokines.
Review
Immunology
Chengshui Liao, Fuchao Mao, Man Qian, Xiaoli Wang
Summary: This review examined the literature on pathogens that produce nucleases to evade the host antimicrobial mechanism mediated by extracellular traps (ETs). It was found that ETs exist in various animals and plants, and they play important roles in immobilizing and killing invading microbes. However, some pathogens can release nucleases to degrade the DNA backbone of ETs, reducing their effectiveness.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Cristina Mesas, Victor Garces, Rosario Martinez, Raul Ortiz, Kevin Doello, Jose M. Dominguez-Vera, Francisco Bermudez, Jesus M. Porres, Maria Lopez-Jurado, Consolacion Melguizo, Jose M. Delgado-Lopez, Jose Prados
Summary: Functionalized amorphous calcium phosphate nanoparticles (ACP NPs) loaded with natural products (esculetin and euphorbetin) showed high selective cytotoxicity against colorectal cancer cells and significant reduction in tumor volume, size, and invasion in vivo. Treatment also increased beneficial bacterial population by restoring antioxidant systems in the colon mucosa of mice.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Otorhinolaryngology
Ping Li, Quanhu Sheng, Li-Ching Huang, Justin H. Turner
Summary: This study characterizes the effect of Pseudomonas aeruginosa-derived flagellin on human sinonasal epithelial cell immune responses. It shows that flagellin can activate a strong innate immune response and induce neutrophilic inflammation. Sinonasal epithelial cells from chronic rhinosinusitis patients have a dysregulated immune response to flagellin, characterized by an imbalance in IL-6 and TNF-alpha secretion.
INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Dahae Lee, Hyejung Jo, Yoojin Jang, Suhyun Bae, Tomoyo Agura, Dongmin Kang, Minsoo Kang, Yuri Kim, Nam-Hyuk Cho, Yejin Kim, Jae Seung Kang
Summary: The use of vaccines is the most effective and reliable method for preventing viral infections, but effective therapeutic agents for treatment after infection are needed. Zanamivir, an inhalable drug, has been used to treat pandemic influenza A/H1N1, but drug-resistant strains can quickly emerge. Alloferon, an immunomodulatory drug, has been found to have antiviral effects against various viruses, including influenza viruses. Therefore, a combination of alloferon and zanamivir was tested to improve antiviral effects and reduce H1N1 resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Po-Sung Fu, Jen-Chyan Wang, Pei-Ling Lai, Shih-Ming Liu, Ya-Shun Chen, Wen-Cheng Chen, Chun-Cheng Hung
Summary: This study evaluated the properties of biodegradable hydrogel beads with calcium phosphate bone cement. The results showed that adding hydrogel beads to the cement did not inhibit cell proliferation and differentiation, and the composite material has potential for dental, craniofacial, and orthopedic applications.
Article
Engineering, Biomedical
Le Thi Trang, Hai Van Le, Sachiko Hiromoto, O. Minho, Equo Kobayashi, Nam Viet Nguyen, Nguyen Quang Cao
Summary: Calcium phosphate (Ca-P) surface coating is an effective method to improve the corrosion resistance and biocompatibility of ZK60 magnesium alloy. This study investigates the effects of different Ca-P coatings on cell behavior and in vivo performance of the coated alloy. The results show that surface factors, particularly coating morphology, play a crucial role in determining cell morphology and density. The Ca-P coating formed at pH 7.8 exhibits the best cellular biocompatibility and enhances the corrosion resistance of the alloy in rabbits.
BIOMEDICAL MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Hailiang Xu, Lei Zhu, Fang Tian, Chengwen Wang, Weidong Wu, Botao Lu, Liang Yan, Shuaijun Jia, Dingjun Hao
Summary: In this study, a novel strontium-modified calcium phosphate cement (SMPC) was developed by introducing tristrontium silicate, which showed better injectability and a shorter setting time compared to traditional calcium phosphate cement (CPC). SMPC also promoted cell proliferation and differentiation, and facilitated bone regeneration in vivo. Therefore, 5% SMPC is a promising substitute material for bone repair with excellent physicochemical properties and biological activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Wenwen Jiang, Li Shi, Lukui Cai, Xiaoyu Wang, Jingyan Li, Heng Li, Jiangli Liang, Qin Gu, Guang Ji, Jing Li, Longding Liu, Mingbo Sun
Summary: The developed candidate vaccine combines N protein and prefusion-full S protein, generating stronger systemic and mucosal humoral immunity through the combination of flagellin and cyclic GMP-AMP. Additionally, administering the candidate vaccine via intranasal route can enhance local immune responses in the respiratory tract and protect against lethal SARS-CoV-2 challenge.
Article
Chemistry, Multidisciplinary
Phil Opitz, Laura Besch, Martin Panthoefer, Anke Kabelitz, Ronald E. Unger, Franziska Emmerling, Mihail Mondeshki, Wolfgang Tremel
Summary: This study reports a protein-free formation of bone-like apatite from amorphous precursors through ball-milling. Mg2+ ions are crucial to achieve complete amorphization of CaCO3, promoting cell growth through stimulating cell viability ex vivo.
ADVANCED FUNCTIONAL MATERIALS
(2021)
