Review
Biochemistry & Molecular Biology
Yulia Suzdaltseva, Sergey L. Kiselev
Summary: Scar formation during normal tissue regeneration in adults can have negative effects on appearance and function. Fetal tissues, on the other hand, are capable of complete regeneration. Understanding the cellular and molecular mechanisms of fetal wound regeneration can lead to successful treatments for minimizing scarring. However, limited access to fetal material hinders research in this area.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Wei Zou, Juanjuan Xing, Shijie Zou, Mei Jiang, Xinping Chen, Qi Chen, Daozheng Liu, Xiangcheng Zhang, Xin Fu
Summary: The study found that HIV-1 (LAI) Nef interferes with the development of HSPCs into myeloid-erythroid lineage cells, thus disrupting hematopoiesis. Therapeutics targeting Nef may hold promise in correcting HIV-1 associated hematopoietic abnormalities.
Article
Cell & Tissue Engineering
Alejandra Vargas-Valderrama, Anne-Charlotte Ponsen, Morgane Le Gall, Denis Clay, Sebastien Jacques, Tudor Manoliu, Valerie Rouffiac, Karine Ser-le-Roux, Cyril Quivoron, Fawzia Louache, Georges Uzan, Maria-Teresa Mitjavila-Garcia, Estelle Oberlin, Hind Guenou
Summary: This study successfully developed a hematoendothelial population that could be further differentiated into endothelial and hematopoietic cells, providing a rapid cell source for future cell therapy and tissue engineering.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biochemistry & Molecular Biology
Ying Yin, Qingming Tang, Mengru Xie, Li Hu, Lili Chen
Summary: Blood vessels in the skeletal system play a critical role in regulating bone development and remodeling through the secretion of signal molecules by endothelial cells. The close contact between endothelial cells and pericytes of blood vessels contributes to osteogenesis and osteoclastogenesis, and extracellular vesicles derived from endothelial cells directly regulate the maturation process of osteoblasts and osteoclasts. Targeting blood vessels as a therapeutic strategy may hold potential for treating bone disorders.
BIOSCIENCE REPORTS
(2021)
Article
Cell Biology
Fatemeh Safi, Parashar Dhapola, Sarah Warsi, Mikael Sommarin, Eva Erlandsson, Jonas Ungerback, Rebecca Warfvinge, Ewa Sitnicka, David Bryder, Charlotta Boiers, Ram Krishna Thakur, Goran Karlsson
Summary: By investigating the chromatin accessibility of single cells, it has been found that there is a population of cells that display both stem-like and lineage-affiliated chromatin signatures. These cells are positioned between stem cells and committed progenitors, showing multi-lineage potential but lacking self-renewal activity.
Article
Cell Biology
Fangyu Wang, Xuan Liu, Shaowen Li, Chen Zhao, Yumei Sun, Kuan Tian, Junbao Wang, Wei Li, Lichao Xu, Jing Jing, Juan Wang, Sylvia M. Evans, Zhiqiang Li, Ying Liu, Yan Zhou
Summary: The possibility of trans-differentiation of GBM cells into vascular cells is unlikely to occur during gliomagenesis, according to the study. The findings advance the understanding of cell lineage dynamics during gliomagenesis and have implications for targeted treatment of GBMs.
Article
Biochemistry & Molecular Biology
Enrico C. Torre, Mesude Bicer, Graeme S. Cottrell, Darius Widera, Francesco Tamagnini
Summary: Adipose-derived mesenchymal stromal cells (ASCs) have the potential to differentiate into various cell types and exhibit spontaneous Ca2+ oscillations. The effects of differentiation treatments on ASCs' Ca2+ dynamics, in terms of oscillation fraction and frequency, were examined in this study. Both adipogenic and osteogenic differentiation resulted in reduced Ca2+ dynamics, with adipogenic differentiation showing a more pronounced effect. Changes in Ca2+ oscillations were observed as early as 7 days in vitro (DIV). Further research is needed to understand the molecular and mechanistic correlates of these changes for the development of interventions in regenerative medicine.
Article
Cell & Tissue Engineering
Chuti Laowtammathron, Chanchao Lorthongpanich, Nittaya Jiamvoraphong, Pimonwan Srisook, Phatchanat Klaihmon, Pakpoom Kheolamai, Sudjit Luanpitpong, Surapol Issaragrisil
Summary: The study explores the role of YAP in hematopoietic differentiation of hiPSCs. It is found that suppressing YAP activity enhances the efficiency of hematopoietic differentiation and increases the yield of HSPCs.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell & Tissue Engineering
Swati Mishra, Jasin Taelman, Mina Popovic, Laurentijn Tilleman, Evi Duthoo, Margot van der Jeught, Dieter Deforce, Filip van Nieuwerburgh, Bjoern Menten, Petra de Sutter, Annekatrien Boel, Susana M. Chuva De Sousa Lopes, Bjorn Heindryckx
Summary: This study found that continuous supplementation of ActA during differentiation of hESCs can promote differentiation into PGCLCs, and conversion to the 4i-state is beneficial for this process. The obtained PGCLCs may further differentiate into mature germ cells.
Article
Biochemistry & Molecular Biology
Jelena Perovanovic, Yifan Wu, Hosiana Abewe, Zuolian Shen, Erik P. Hughes, Jason Gertz, Mahesh B. Chandrasekharan, Dean Tantin
Summary: The transition between pluripotent and tissue-specific states is crucial for development, and understanding the underlying mechanisms can aid in the engineering of properly differentiated cells. This study identified Oct1 as a key mediator of mesoderm lineage-specific gene induction. Oct1 deficiency resulted in impaired mesodermal and terminal muscle differentiation, as well as poorly coordinated induction of lineage-specific genes and inappropriate lineage branching. Oct1 was found to interact with the histone lysine demethylase Utx, suggesting their cooperation in activating gene expression.
Article
Cardiac & Cardiovascular Systems
Arash Pezhouman, James L. Engel, Ngoc B. Nguyen, Rhys J. P. Skelton, William Blake Gilmore, Rong Qiao, Debashis Sahoo, Peng Zhao, David A. Elliott, Reza Ardehali
Summary: In this study, we successfully isolated and characterized first and second heart field- and nodal-like cardiomyocytes from human embryonic stem cells using a double reporter line. Our findings provide a comprehensive understanding of the early development and functional properties of these different cardiomyocyte populations. We also identified two novel surface markers that can be used for purification of cardiomyocytes. This research has important implications for gene expression profiling, arrhythmia modeling, and drug screening, and it paves the way for the development of stem cell therapy for specific heart conditions.
CARDIOVASCULAR RESEARCH
(2022)
Article
Multidisciplinary Sciences
Leal Oburoglu, Els Mansell, Niels-Bjarne Woods
Summary: Glutamine metabolism plays an essential role in regulating the emergence and specification of hematopoietic cells during endothelial to hematopoietic transition (EHT), with different hematopoietic lineages requiring distinct derivatives of glutamine.
SCIENTIFIC REPORTS
(2021)
Article
Cell & Tissue Engineering
Idil I. Aigha, Essam M. Abdelalim
Summary: The aim of this study was to examine the effect of p53 inhibition on the generation of PDX1(+)/NKX6.1(+) pancreatic progenitor cells and pancreatic beta-cell generation. The results showed that inhibition of p53 increased the number of PDX1(+)/NKX6.1(+) cells and reduced the number of CHGA(+)/NKX6.1(-) cells. Furthermore, the use of a p53 inhibitor during differentiation of pancreatic progenitor cells led to a decrease in the generation of polyhormonal beta-cells.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Cell & Tissue Engineering
Ece Somuncular, Julia Hauenstein, Prajakta Khalkar, Anne-Sofie Johansson, Ozge Dumral, Nicolai S. Frengen, Charlotte Gustafsson, Giuseppe Mocci, Tsu-Yi Su, Hugo Brouwer, Christine L. Trautmann, Michael Vanlandewijck, Stuart H. Orkin, Robert Mansson, Sidinh Luc
Summary: This study reveals the heterogeneity of hematopoietic stem cells (HSCs) and provides insights into the functional differences and regulatory mechanisms of HSCs through transcriptional and epigenetic mechanisms.
Article
Hematology
Emanuele Gioacchino, Cansu Koyunlar, Joke Zink, Hans de Looper, Madelon de Jong, Tomasz Dobrzycki, Christopher B. Mahony, Remco Hoogenboezem, Dennis Bosch, Paulina M. H. van Strien, Martin E. van Royen, Pim J. French, Eric Bindels, Kirsten J. Gussinklo, Rui Monteiro, Ivo P. Touw, Emma de Pater
Summary: Through studying Gata2b-deficient zebrafish, this research demonstrates the role of GATA2 in hematopoiesis, showing a decrease in myeloid lineage differentiation and a shift towards lymphoid lineage differentiation in the absence of Gata2b.