Review
Immunology
Dane Kim, Alisa E. Lee, Qilin Xu, Qunzhou Zhang, Anh D. Le
Summary: GMSCs, a unique subpopulation of mesenchymal stem cells isolated from human gingival tissues, possess potent regenerative potential and the ability to differentiate into neural cell lineages. They also exhibit strong immunomodulatory and anti-inflammatory effects, making them a promising source for tissue engineering and regenerative therapy.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Alexandra Roi, Ciprian Roi, Meda Lavinia Negrutiu, Laura Cristina Rusu, Mircea Rivis
Summary: Dental stem cells, such as human periapical cyst mesenchymal stem cells (hPCy-MSCs), derived from the oral cavity, possess multilineage differentiation potential, immunomodulatory activity, and repair capacity. These cells exhibit similar characteristics to mesenchymal stem cells (MSCs) and hold great significance for the development of clinical applications in dentistry.
Article
Biochemistry & Molecular Biology
Nerea Sanchez, Fabio Vignoletti, Ignacio Sanz-Martin, Alejandro Coca, Javier Nunez, Estela Maldonado, Javier Sanz-Esporrin, Irene Hernando-Pradies, Silvia Santamaria, David Herrera, Jose A. Garcia-Sanz, Mariano Sanz
Summary: The combination of xenogeneic collagen matrix and autologous GMSCs with a coronally advanced flap showed promising results in reducing inflammation, promoting new cementum/bone formation, and reducing gingival recession compared to other treatment groups, although the differences were not statistically significant.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Avinash Kharat, Avinash Sanap, Supriya Kheur, Madhura Shekatkar, Ramesh Bhonde
Summary: This study demonstrates that human gingiva-derived mesenchymal stem cells (hGMSCs) can differentiate into Insulin Producing Cell Clusters (IPCCs), providing a new autologous tissue source for screening anti-diabetic drugs and islet transplantation for the treatment of diabetes.
MOLECULAR BIOLOGY REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Luigia Fonticoli, Ylenia Della Rocca, Thangavelu Soundara Rajan, Giovanna Murmura, Oriana Trubiani, Stefano Oliva, Jacopo Pizzicannella, Guya Diletta Marconi, Francesca Diomede
Summary: Gingival tissue can be collected easily and used as a source to isolate GMSCs, which possess differentiation and immunomodulatory abilities similar to MSCs. GMSCs can be expanded in vitro while maintaining their stemness properties. Furthermore, the EVs released from GMSCs have shown similar biological functions and therapeutic effects, making them a promising tool for cell-free regenerative therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Sichen Zhang, Dashuai Zhu, Zhenhua Li, Ke Huang, Shiqi Hu, Halle Lutz, Mengjie Xie, Xuan Mei, Junlang Li, Genevieve Neal-Perry, Shaowei Wang, Ke Cheng
Summary: The therapeutic ovarian regenerative patch (ORP) has been found to effectively treat POI and restore fertility in rats by stimulating the proliferation of ovarian somatic cells and inhibiting apoptosis under injury stress. This cell-free, off-the-shelf, and clinically feasible treatment represents a promising approach for POI.
Review
Cell Biology
Asmaa Fadl, Andrew Leask
Summary: Adult human gingival fibroblasts (HGFs) are important for maintaining oral homeostasis and have great potential for tissue engineering and regeneration applications. They have unique characteristics such as resistance to myofibroblast differentiation, immunomodulation, high regenerative capacity, and responsiveness to growth factors and cytokines. This review highlights in vitro and translational studies on the regenerative and differentiation potential of HGFs and emphasizes the need for further research to translate their potential into clinical applications.
Article
Chemistry, Multidisciplinary
Yaser A. Alhazmi, Mohammed Y. Aljabri, Shereen N. Raafat, Shaimaa M. Gomaa, Mohamed Shamel
Summary: This study aimed to examine the impact of low-level laser therapy (LLLT) on the viability and osteo/odontogenic potential of gingival-derived mesenchymal stem cells (GMSCs). The results showed that LLLT of both energy densities had no cytotoxic effect on GMSC viability. LLLT of 1.5 J/cm(2) demonstrated better viability and promoted osteo/odontogenic differentiation potential of GMSCs.
APPLIED SCIENCES-BASEL
(2023)
Article
Pharmacology & Pharmacy
Talagavadi Channaiah Anudeep, Madhan Jeyaraman, Sathish Muthu, Ramya Lakshmi Rajendran, Prakash Gangadaran, Prabhu Chandra Mishra, Shilpa Sharma, Saurabh Kumar Jha, Byeong-Cheol Ahn
Summary: Alopecia, including scarring and non-scarring types, is a common diagnosis in clinical practice. The most prevalent type is non-scarring alopecia, with androgenetic alopecia and alopecia areata being the majority of cases. Traditional treatments have significant downsides, leading to the exploration of regenerative therapies. Growth factor-rich and stem cell-rich treatments have shown potential, but standardization is lacking. Further understanding and standardization of regenerative cellular therapies for hair restoration are needed.
Review
Biochemistry & Molecular Biology
Igor Smojver, Ivan Katalinic, Roko Bjelica, Dragana Gabric, Vid Matisic, Vilim Molnar, Dragan Primorac
Summary: The application of mesenchymal stem cells (MSC) in regenerative dentistry is gaining importance. Dental tissue-derived MSCs have characteristics such as multidifferentiation potential, self-renewal capacity, and easy accessibility, making them key players in stem cell-based therapy. Different types of dental stem cells have been discovered and their potential usage is found in various branches of dentistry as well as other medical fields. This review focuses on dental MSC sources and their treatment modalities, with a specific emphasis on temporomandibular joint osteoarthritis (TMJ OA).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Michal Domaniza, Marian Hluchy, Dasa Cizkova, Filip Humenik, Lucia Slovinska, Nikola Hudakova, Lubica Hornakova, Juraj Vozar, Alexandra Trbolova
Summary: This study aimed to investigate the potential of cell-based regenerative therapy for elbow joints affected by osteoarthritis. The results showed that two injections of amnion-derived mesenchymal stem cells improved joint range of motion and had an impact on synovial fluid biomarkers. Concentrations of IFN-γ, IL-6, and TNF-α decreased while IL-10 increased between day 0 and day 28 after treatment.
Article
Chemistry, Medicinal
Aleksandra Skubis-Sikora, Bartosz Sikora, Weronika Malysiak, Patrycja Wieczorek, Piotr Czekaj
Summary: Melatonin has been evaluated for its safety and biological activity on ADSCs in vitro, and it was found that low-dose melatonin can improve cell viability without negatively affecting osteogenic potential.
Article
Dentistry, Oral Surgery & Medicine
Alice Blufstein, Christian Behm, Barbara Kubin, Johannes Gahn, Andreas Moritz, Xiaohui Rausch-Fan, Oleh Andrukhov
Summary: This study reveals the immunomodulatory properties of GMSCs towards PMNs, with IL-1 beta enhancing their anti-apoptotic effects. However, GMSCs did not affect the ROS production of PMNs.
Review
Cell Biology
Luigino Calzetta, Marina Aiello, Annalisa Frizzelli, Francesca Camardelli, Mario Cazzola, Paola Rogliani, Alfredo Chetta
Summary: This study found that stem cell-based regenerative therapies and derived products may be effective in improving lung function and walking distance in COPD patients, but did not reduce the risk of hospitalization due to acute exacerbations. Further large and well-designed randomized controlled trials are needed to assess the beneficial impact of these therapies in COPD.
Article
Cell & Tissue Engineering
Satoshi Hosoya, Ryo Yokomizo, Harue Kishigami, Yukiko Fujiki, Erika Kaneko, Mitsuyoshi Amita, Takakazu Saito, Hiroshi Kishi, Haruhiko Sago, Aikou Okamoto, Akihiro Umezawa
Summary: Menstrual blood-derived cells from infertile patients have been proven to possess mesenchymal stem cell characteristics, and their proliferative and paracrine capacities are comparable to those from healthy individuals. The intrauterine transplantation of these cells in a mouse model demonstrated their regenerative potential and therapeutic effects.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell & Tissue Engineering
Muhammad A. Awadeen, Fouad A. Al-Belasy, Laila E. Ameen, Mohamad E. Helal, Mohammed E. Grawish
WORLD JOURNAL OF STEM CELLS
(2020)
Review
Cell & Tissue Engineering
Mohammed E. Grawish, Lamyaa M. Grawish, Hala M. Grawish, Mahmoud M. Grawish, Salwa A. El-Negoly
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2020)
Article
Biotechnology & Applied Microbiology
Basmah El-Sayed, Robert Philip Wynn Davies, Rehab R. El-Zehery, Fatma Mohamed Ibrahim, Mohammed E. Grawish, Jennifer Kirkham, Reem El-Gendy
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Article
Dentistry, Oral Surgery & Medicine
Salma M. Fathy, Walid Al-Zordk, Mohammed E. Grawish, Micheal Swain
Summary: Recent improvements in monolithic zirconia with higher yttria content have shown significant enhancements in translucency and flexural strength. However, compared to glass ceramics, monolithic zirconia still lacks unique translucency and may sacrifice some mechanical performance while improving aesthetically. Careful consideration is needed when using thin sections of aesthetic zirconia structures, especially in high bearing stress areas.
Article
Dentistry, Oral Surgery & Medicine
A. Abou ElReash, H. Hamama, M. Grawish, M. Saeed, A. M. Zaen El-Din, M. A. Shahin, W. Zhenhuan, X. Xiaoli
Summary: In a laboratory setting, ACTIVA, MTA-HP, and iRoot-BP-Plus demonstrated significant promotion of hDPSCs proliferation, mineralization, and attachment compared to the control group. iRoot-BP-Plus surfaces showed the highest cellular attachment, followed by MTA-HP, while ACTIVA group exhibited the lowest attachment. Significant differences were observed in mineralizing nodule formation among different experimental groups.
INTERNATIONAL ENDODONTIC JOURNAL
(2021)
Article
Dentistry, Oral Surgery & Medicine
Hanan A. Soliman, Radwa Ibrahim El-Toukhy, Mona Mohsen Ebrahim, Mohammed E. Grawish, Mohamed Abdel Kader Sobh, Salah Hasab Mahmoud
Summary: The study found that a combination of the immunosuppressive drugs Pred, CsA, and MMF had a negative impact on pulp healing, while treatment with Pred alone showed no significant difference compared to the control group.
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Alaa M. Mansour, Sarah Yahia, Hassan Reda Hassan Elsayed, Saied A. E. El-Attar, Mohammed E. Grawish, Youssry M. El-Hawary, Ibrahim M. El-Sherbiny
Summary: The newly developed PCL-based nanofibrous composite scaffold showed enhanced periodontal wound healing/regeneration of class II furcation defects. Moreover, the combination of ADSCs seeded onto the scaffold further enhanced the periodontal tissues regeneration, demonstrating significant improvements in defect dimensions and tissue quality compared to the control group.
CLINICAL ORAL INVESTIGATIONS
(2022)
Review
Cell & Tissue Engineering
Mohammed E. Grawish, Lamyaa M. Grawish, Hala M. Grawish, Mahmoud M. Grawish, Ahmed A. Holiel, Nessma Sultan, Salwa A. El-Negoly
Summary: Dentin, as a permeable tubular composite, can be demineralized to create a bioactive material called demineralized dentin matrix (DDM). DDM has shown potential in enhancing dental and alveolar bone tissue regeneration. Various forms and shapes of DDM have been studied, and it has been applied in different regenerative procedures, including dentin-pulp complex regeneration and bone and cartilage healing. Further research is needed to establish standardized procedures for specific applications of DDM.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Correction
Cell & Tissue Engineering
Mohammed E. Grawish, Lamyaa M. Grawish, Hala M. Grawish, Mahmoud M. Grawish, Ahmed A. Holiel, Nessma Sultan, Salwa A. El-Negoly
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Dentistry, Oral Surgery & Medicine
Elham H. Ahmed, Mohammed E. Grawish, Mohamed M. Anees, Menatalla M. Elhindawy, Mohamed Abdulrahman, Mohamad E. Helal
Summary: This study aimed to demonstrate the changes induced by cadmium intoxication and the possible prophylactic and therapeutic effects of bone marrow mesenchymal stem cells (BMSCs) in rat submandibular salivary glands. The results showed that the prophylactic and therapeutic groups had similar histological and biochemical indicators compared to the control group, indicating that BMSCs have prophylactic and therapeutic effects against cadmium-induced cytotoxicity.
ARCHIVES OF ORAL BIOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Hala H. Hazzaa, Mai S. Attia, Marwa A. M. El Shiekh, Mohammed E. Grawish, Mohamed Moawed I. Ghoneim, Nahed M. Adly, Noha S. Shams, Mohamed A. El-Mahdy, Gasser M. Elewa
Summary: The study investigated the effect of intramarrow penetration (IMP) and 1% melatonin (MLN) gel on the remodelling process of autogenous bone graft (ABG) in an induced 1-osseous wall defect model. The results showed that using IMP and 1% MLN gel as adjuncts to ABG can increase bone fill and reduce endothelial nitric oxide synthase (eNOS) expression.
INTERNATIONAL DENTAL JOURNAL
(2023)
Article
Multidisciplinary Sciences
Ashraf Abou ElReash, Mohamed Grawish, Walied Abdo, Amr M. Abdelghany, Luo Junsi, Xie Xiaoli, Hamdi Hamama
Summary: This study compared the bone tissue reaction of three materials and found that calcium silicate-based cement induced more bone formation compared to an ion-releasing resin modified glass-ionomer cement. However, the bio-inductive properties of the three materials were similar. This suggests that bioactive resin composite can be used as a retrograde filling material.
SCIENTIFIC REPORTS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Salma Alaa, Ahmed Maher Fouda, Mohammed E. Grawish, Yasser Lotfy Abdelnaby
Summary: This study aimed to compare the effects of submucosal injection of platelet rich fibrin (i-PRF) versus platelet rich plasma (PRP) on orthodontic tooth movement (OTM) in a rabbit model over a 28-day follow-up period. The results showed significant differences in tooth movement distance and periodontal ligament width between the control group and the groups receiving PRP and i-PRF injections, with i-PRF having a more pronounced effect. Submucosal injection of PRP or i-PRF is a minimally invasive and safe approach for accelerating OTM.
INTERNATIONAL ORTHODONTICS
(2023)
Article
Cell Biology
Nessma Sultan, Laila E. Amin, Ahmed R. Zaher, Mohammed E. Grawish, Ben A. Scheven
Summary: Dental pulp stem cells secrete neurotrophic factors that significantly promote cell survival and neurite outgrowth in PC12 cells, with NGF, BDNF, and GDNF playing important roles. These factors may have potential therapeutic applications for peripheral nerve repair.
NEURAL REGENERATION RESEARCH
(2021)
Article
Multidisciplinary Sciences
Nessma Sultan, Laila E. Amin, Ahmed R. Zaher, Mohammed E. Grawish, Ben A. Scheven
SCIENTIFIC REPORTS
(2020)
