Review
Biochemistry & Molecular Biology
Samantha Donsante, Biagio Palmisano, Marta Serafini, Pamela G. Robey, Alessandro Corsi, Mara Riminucci
Summary: Bone formation begins in the embryonic stage and continues throughout life, involving stages of bone modeling and growth, remodeling, and regeneration. Osteoblasts produce, assemble, and control mineralization of the bone matrix, while regulating other cell processes and metabolic activities. Osteoblasts are derived from different skeletal stem cells under the control of multiple regulators at different developmental stages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Marianna Cosentino, Carmine Nicoletti, Valentina Valenti, Leonardo Schirone, Flavio Di Nonno, Ludovica Apa, Mariam Zouhair, Desiree Genovese, Luca Madaro, Simone Dinarelli, Marco Rossi, Zaccaria Del Prete, Sebastiano Sciarretta, Giacomo Frati, Emanuele Rizzuto, Antonio Musaro
Summary: The adult heart has poor reparative capacities after injury. Cell transplantation and tissue engineering have emerged as possible therapeutic options. In this study, a new experimental tool called 3D eX vivo muscle engineered tissue (X-MET) was used to investigate the role of mechanical stimuli in functional remodeling and cardiac ischemia rescue.
SCIENTIFIC REPORTS
(2023)
Editorial Material
Biochemistry & Molecular Biology
Daniel E. Michele
Summary: Skeletal muscle is a remarkable tissue that can protect and repair itself through the activation of satellite cells. The loss of these mechanisms may contribute to muscle diseases and age-related decline in muscle function.
Article
Food Science & Technology
Enbo Xu, Ruihao Niu, Jihui Lao, Shengliang Zhang, Jie Li, Yiyuan Zhu, Huimin Shi, Qingqing Zhu, Yijian Chen, Yuyan Jiang, Wenjun Wang, Jun Yin, Qihe Chen, Xiao Huang, Jun Chen, Donghong Liu
Summary: Tissue-like fish fillets were successfully developed by assembling large yellow croaker muscle fibers and adipocytes with 3D-printed gel. Inhibiting Tgf-beta and Notch signals promoted myogenic differentiation of piscine satellite cells (PSCs), while a mixture of fish gelatin, sodium alginate, a p53 inhibitor, and a Yap activator supported PSC viability and proliferation. A 3D scaffold was constructed using gelatin-based gel mixed with PSCs, and after proliferation and differentiation, the scaffold was filled with cultured piscine adipocytes. The resulting tissue-like fish fillets consisted of 5.67 x 10(7) muscles and 4.02 x 10(7) adipocytes, measuring 20 x 12 x 4 mm. Biomanufacturing tissue-like cultured fish fillets holds promise for customized meat production.
NPJ SCIENCE OF FOOD
(2023)
Review
Biochemistry & Molecular Biology
Li Huang, Ming Li, Chunyan Deng, Jiayi Qiu, Kexin Wang, Mengyuan Chang, Songlin Zhou, Yun Gu, Yuntian Shen, Wei Wang, Ziwei Huang, Hualin Sun
Summary: The maintenance of muscle homeostasis is crucial for life and health. Skeletal muscle atrophy not only reduces people's quality of life and increases morbidity and mortality, but also causes a significant socioeconomic burden. Unfortunately, no effective treatment has been developed for skeletal muscle atrophy due to an incomplete understanding of its molecular mechanisms.
Article
Multidisciplinary Sciences
Jun-Ha Hwang, Kyung Min Kim, Ho Taek Oh, Gi Don Yoo, Mi Gyeong Jeong, Hyun Lee, Joori Park, Kwon Jeong, Yoon Ki Kim, Young-Gyu Ko, Eun Sook Hwang, Jeong-Ho Hong
Summary: This study reveals that TAZ is a novel stimulator for mitochondrial biogenesis and exercise-induced muscle adaptation. TAZ stimulates the translation of mitochondrial transcription factor A via the Rheb/Rhebl1-mTOR pathway. The knockout of TAZ in mice results in decreased mitochondrial biogenesis, respiratory metabolism, and exercise ability.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Ya Guan, Ning Gao, Hong Niu, Yu Dang, Jianjun Guan
Summary: The developed oxygen-release microspheres have the potential to enhance cell survival in ischemic tissues by improving oxygen supply, promoting angiogenesis, and tissue regeneration in a safer and more efficient manner. The microspheres are responsive to environmental oxygen levels, controlling oxygen release and significantly improving the therapeutic effects of cell transplantation under hypoxic conditions.
JOURNAL OF CONTROLLED RELEASE
(2021)
Review
Biochemistry & Molecular Biology
HoTae Lim, In Young Choi, Sang-Hwan Hyun, Hyesoo Kim, Gabsang Lee
Summary: Using hPSCs to generate skeletal muscle tissues in vitro provides a valuable model for understanding molecular functions and gene regulatory networks involved in muscle differentiation and disease pathology. The combination of established myogenesis protocols and molecular profiling techniques offers insights into normal and disease skeletal muscle development.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Zaheer Ahmed, Rizwan Qaisar
Summary: This review highlights recent research on the application of nanomedicine in treating muscular dystrophies. The article discusses the history and broad applications of nanomedicine, as well as the use of nanoparticles for drug delivery, gene regulation, and editing to target Duchenne muscular dystrophy and myotonic dystrophy. The potential hindrances and limitations of using nanomedicine in cell culture and animal models are also addressed. Finally, the future perspectives for using nanomedicine in clinics for muscular dystrophies are summarized.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Pawandeep Singh, Benedicte Chazaud
Summary: This article discusses the regeneration process of adult skeletal muscle after injury and the role of immune cells, particularly macrophages, in supporting muscle regeneration. It highlights the issues caused by inflammation in muscle diseases and explores how targeting the inflammatory compartment can improve muscle regeneration.
EXPERIMENTAL CELL RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Fasih Ahmad Rahman, Joe Quadrilatero
Summary: Remodeling of the mitochondrial network plays a critical role in maintaining cellular homeostasis and regulating skeletal muscle regeneration. The balance between biogenesis and mitophagy, as well as mitochondrial fission and fusion, are key processes in mitochondrial network remodeling.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Cell Biology
Magdaleena Naemi Mbadhi, Jun-ming Tang, Jing-xuan Zhang
Summary: Satellite stem cells with high regenerative capacity are ideal for treating muscular diseases. The regulation of myogenic regulatory factors plays a crucial role in the differentiation of satellite stem cells into skeletal muscles. While progress has been made in using HDACi, further research is needed on histone methylation modulations and lncRNA.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Adrian Pickar-Oliver, Veronica Gough, Joel D. Bohning, Siyan Liu, Jacqueline N. Robinson-Hamm, Heather Daniels, William H. Majoros, Garth Devlin, Aravind Asokan, Charles A. Gersbach
Summary: Targeted gene-editing strategies offer promising approaches for treating genetic diseases, with HITI showing potential for inserting DNA sequences at specific genomic loci. This method has garnered attention for its potential to correct a variety of DMD mutations.
Article
Biology
Xiaoling Zeng, Luyuan Xie, Yuxin Ge, Yue Zhou, Hui Wang, Yongyi Chen, Xiaomei Zhu, Huayun Liu, Qianjin Liao, Yu Kong, Lijun Pan, Junjun Li, Lei Xue, Sha Li, Xiao Zhou, Chunmeng Shi, Xiaowu Sheng
Summary: This study provides cellular evidence of satellite cell activation and proliferation in radiation-induced muscle fibrosis in rats. The study examines the effects of radiation on satellite cell proliferation and differentiation, as well as the associated mechanisms such as apoptosis, autophagy, and changes in cytokines.
RADIATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yundong Peng, Jingjing Du, Stefan Gunther, Xinyue Guo, Shengpeng Wang, Andre Schneider, Li Zhu, Thomas Braun
Summary: Skeletal muscle stem cells, also known as satellite cells, play a crucial role in postnatal muscle growth and regeneration. This study reveals that Piezo1, a mechanosensitive ion channel, is essential for maintaining the quiescence and preventing senescence of muscle stem cells. Absence of Piezo1 leads to premature activation of these cells, impairs their proliferation and differentiation, and significantly hinders skeletal muscle regeneration. The inactivation of Piezo1 results in compensatory up-regulation of T-type voltage-gated Ca2+ channels, which increases the influx of Ca2+ and induces NOX4 expression through cPKC. The elevated expression of NOX4 in Piezo1-deficient cells leads to increased levels of reactive oxygen species (ROS) and DNA damage, causing P53-dependent cellular senescence and cell death. The study also highlights the importance of the P53/P21-axis in mediating the cellular defects caused by the absence of Piezo1. This research sheds light on the critical role of Piezo1-mediated mechano-signaling in maintaining the quiescence and preventing senescence of muscle stem cells, and suggests that reduced mechano-signaling during aging may contribute to the accumulation of senescent cells and the decline of muscle stem cell numbers in geriatric individuals.