Article
Biochemistry & Molecular Biology
Elena Vargas-Accarino, Carlos Herrera-Montavez, Santiago Ramon y Cajal, Trond Aasen
Summary: Our study demonstrates that dynamic subpopulations of free-floating and adherent cells serve as a useful model for screening and identifying factors that regulate the process of cancer metastasis. Shared differentially expressed genes among the three cancer cell lines provide directions for further research. Cellular responses to hypoxia and regulation through pathways such as mTOR and cell adhesion have impacts on metastasis and malignancy processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Matthew J. White, Kyle A. Jacobs, Tania Singh, Lakyn N. Mayo, Annie Lin, Christopher S. Chen, Young-wook Jun, Matthew L. Kutys
Summary: This study uses a 3D model to demonstrate that Notch1 influences epithelial morphogenesis through a mechanism independent of transcriptional signaling. Notch1 cortical signaling directly regulates epithelial cell architecture and proliferation by stabilizing adherens junctions and cortical actin. These findings provide new insights into Notch1 signaling and regulation and suggest a coordination between transcriptional and cell adhesive programs.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Taryn D. Treger, John E. G. Lawrence, Nathaniel D. Anderson, Tim H. H. Coorens, Aleksandra Letunovska, Emilie Abby, Henry Lee-Six, Thomas R. W. Oliver, Reem Al-Saadi, Kjell Tullus, Guillaume Morcrette, J. Ciaran Hutchinson, Dyanne Rampling, Neil Sebire, Kathy Pritchard-Jones, Matthew D. Young, Thomas J. Mitchell, Philip H. Jones, Maxine Tran, Sam Behjati, Tanzina Chowdhury
Summary: In this study, we examined somatic changes in reninoma and discovered structural variants involving NOTCH1 and NRARP, resulting in excessive renin and NOTCH1 signaling but normal expression of NRARP. Additionally, our findings were supported by previous studies that also found dysregulated Notch pathway signaling in reninoma.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Sang-Yun Lee, Hyun Ju Hwang, Bosung Ku, Dong Woo Lee
Summary: The use of the CPRE 3D-HTS model improves the correlation of EGFR-targeting drugs compared to 2D cell-based HTS. In breast cancer cell lines, the CPRE 3D-HTS model shows a greater difference in the expression of HER2 protein between HER2-positive and HER2-negative cells. Alginate was selected as the extracellular matrix to enhance cell proliferation receptor expression in the CPRE 3D-HTS model.
ANALYTICAL CHEMISTRY
(2022)
Article
Cell Biology
Mian Xie, Xin-ge Fu, Ke Jiang
Summary: The interaction between Notch1 and TAZ promotes aerobic glycolysis and immune escape in lung cancer, providing potential therapeutic targets against Notch1 and TAZ for clinical translation in lung cancer.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Junyao Wang, Wenjing Peng, Aiying Yu, Mohamed Fokar, Yehia Mechref
Summary: Plasmax affects cellular behaviors differently in two cancer cell lines, with inhibited invasion and cell death in MDA-MB-231BR, and activated invasion, migration, and protein synthesis in CRL-1620. Plasmax has a greater influence on protein regulatory processes post-translation compared to its effect on transcripts, providing complementary information for selecting appropriate media for in vitro cancer cell studies.
Article
Cell Biology
Joanna Kalafut, Jakub Czapinski, Alicja Przybyszewska-Podstawka, Arkadiusz Czerwonka, Adrian Odrzywolski, Cecilia Sahlgren, Adolfo Rivero-Muller
Summary: The Notch signaling pathway plays a crucial role in cell differentiation and tissue organization, and its dysregulation is linked to breast cancer progression. By engineering an optogenetic receptor, we were able to control the activity of Notch signaling using light and show its effects on cell proliferation, migration, and chemoresistance in breast cancer cells.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Oncology
Shinsuke Suzuki, Sawako Hourai, Kimiharu Uozumi, Yuichirou Uchida, Makoto Yoshimitsu, Hachiman Miho, Naomichi Arima, Shin-Ichi Ueno, Kenji Ishitsuka
Summary: This study reveals that NOTCH1 protein is constitutively activated but likely a passenger in NOTCH1-mutation-negative ATL cell proliferation. Furthermore, GSI does not suppress the growth of ATL cell lines.
Article
Biochemistry & Molecular Biology
Yoel Genaro Montoyo-Pujol, Marta Garcia-Escolano, Jose J. Ponce, Silvia Delgado-Garcia, Tina Aurora Martin, Hortensia Ballester, Elena Castellon-Molla, Pascual Martinez-Peinado, Sandra Pascual-Garcia, Jose Miguel Sempere-Ortells, Gloria Peiro
Summary: Advances in immunotherapy have led to increased interest in studying the role of the immune system in breast cancer. This study evaluated the expression of various immune regulation genes in different breast cancer cell lines and mammospheres. The results showed that the expression of these genes varied depending on the breast cancer phenotype, culture conditions, and interaction with immune cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Rashedul Islam, Catherine E. Jenkins, Qi Cao, Jasper Wong, Misha Bilenky, Annaick Carles, Michelle Moksa, Andrew P. Weng, Martin Hirst
Summary: Runt-related transcription factor 1 (RUNX1) is oncogenic in various leukemias and epithelial cancers, and its expression is associated with poor prognosis. It cooperates with other oncogenic factors to drive the expression of proto-oncogenes in T cell acute lymphoblastic leukemia (T-ALL), but the specific molecular mechanisms and cooperation with other factors are not well understood.
Article
Polymer Science
Lixia Huang, Yifei Jiang, Xinying Chen, Wenqi Zhang, Qiuchen Luo, Siyan Chen, Shuhan Wang, Fangqing Weng, Lin Xiao
Summary: Supramolecular responsive microcarriers based on chitosan microspheres were developed for nonenzymatic cell detachment. These microcarriers showed excellent biocompatibility and gelatin niches, allowing for effective cell attachment and expansion. Cells could be detached from the microcarriers through competitive binding.
Review
Anatomy & Morphology
A. Thirumal Raj, Supriya Kheur, Ramesh Bhonde, Archana A. Gupta, Shankargouda Patil
Summary: This study assessed the effect of human MSC derived conditioned media on human cancer cell lines, identifying pro-carcinogenic and anti-carcinogenic molecules and effector pathways associated with different MSC types. The use of MSC-CM with pro-carcinogenic effect should be restricted in cancer patients regardless of the application nature.
Article
Oncology
Goksu Kasarci, Baris Ertugrul, Elif Sinem Iplik, Bedia Cakmakoglu
Summary: Studies have demonstrated that succinic acid exhibits significant cytotoxic and apoptotic activity on renal cancer cells, while having no significant impact on healthy cells. This suggests that succinic acid could be a potential therapeutic agent for individualized cancer treatment approaches, warranting further molecular research.
Review
Biotechnology & Applied Microbiology
Christine Yee, Kristie-Ann Dickson, Mohammed N. Muntasir, Yue Ma, Deborah J. Marsh
Summary: Ovarian cancer has the highest mortality rate among gynecological malignancies. Different histotypes of the cancer respond differently to treatments, with chemoresistance being a key factor in incurable disease. While PARP inhibitors and bevacizumab are currently used in clinical settings, further research is needed to predict patient responses to these drugs effectively.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Jennifer Edelmann, Arran D. Dokal, Emma Vilventhraraja, Karlheinz Holzmann, David Britton, Tetyana Klymenko, Hartmut Doehner, Mark Cragg, Andrejs Braun, Pedro Cutillas, John G. Gribben
Summary: Obinutuzumab and rituximab differ in activating pathways downstream of the B cell receptor, with obinutuzumab inducing greater apoptosis through aberrant SYK phosphorylation while rituximab enhances anti-apoptotic signals through different mechanisms. The complexity and differences in their interference with signaling pathways essential for lymphoma treatment underscore the importance of optimizing and personalizing the application of different anti-CD20 treatments.
