Journal
ACTA PHARMACEUTICA SINICA B
Volume 3, Issue 5, Pages 337-344Publisher
INST MATERIA MEDICA, CHINESE ACAD MEDICAL SCIENCES
DOI: 10.1016/j.apsb.2013.08.002
Keywords
Radix Isatidis; Glucosinolate; Nucleoside; R.S-Goitrin; Quality control; HPLC-DAD-ESI/MS
Categories
Funding
- Special Program for New Drug Innovation of the Ministry of Science and Technology, China [2011ZX09201-201-12, 2011ZX09201-201-07]
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Multi-component fingerprinting and quathitation of the glucosinolates and nucleosides in samples of Radix Isatidis have been carried out using high-performance liquid chromatography with diode-array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI/MS). Five nucleosides together with one glueosinolate were identified by comparing retention times, ultraviolet spectra, mass spectra and/or empirical molecular formulae of reference compounds. Quantitation of these six compounds was carried out simultaneously by HPLC on a Phenomenex Luna C18 column using gradient elution with methanol and water and detection at 254 am. All calibration curves were linear (r > 0.9994) within test ranges. Limits of detection and quantitation were 0.33 ng and 2.50 ng on column, respectively. Intra- and inter-day precision (as relative standard deviation) for all analytes was <2.19% with recoveries in the range 99.6%-101.8% at three concentration levels. The validated method was successfully applied to fingerprinting and assay of 25 batches of Radix Isatidis sourced from different geographical regions of China. The method is simple and reliable and has potential value in the quality control of Radix Isatidis. (C) 2013 Institute of Maeda Medica, Chinese Academy of Medical Sciences and Chinese Pharmaceutical Association. Production and hosting by Elsevier B.V. All rights reserved.
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