Article
Engineering, Biomedical
Dipasha Sinha, Agnes Nagy-Mehesz, Dan Simionescu, John E. Mayer Mayer Jr, Naren Vyavahare
Summary: Congenital heart diseases (CHD) are common disorders affecting approximately 40,000 newborns annually in the United States. A new substitute conduit artery called PGG-DBJVC has been developed, which exhibits good biocompatibility, resistance to calcification, and adequate functionality in animal experiments.
ACTA BIOMATERIALIA
(2023)
Review
Engineering, Biomedical
Leanne de Silva, Paulina N. Bernal, A. J. W. Rosenberg, Jos Malda, Riccardo Levato, Debby Gawlitta
Summary: The development of tissue engineering strategies for treatment of large bone defects is crucial, and creating a vascular network is vital for tissue engineered bone constructs.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Sangil Min, David Cleveland, In Kap Ko, Ji Hyun Kim, Hee Jo Yang, Anthony Atala, James J. Yoo
Summary: By incorporating vascular endothelial growth factor (VEGF) into the vascular scaffold, improved angiogenic capability was achieved, promoting vascularization, reducing apoptosis of implanted cells, and forming hybrid renal tubule-like structures.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Biomedical
Hong zhen Xu, Jian sheng Su
Summary: The success of repairing large bone defects using tissue engineering technology depends on angiogenesis and osteogenesis. This study successfully prepared composite scaffolds loaded with small vascular stents, combined with osteoblasts and vascular endothelial cells, to repair critical mandibular defects in rabbits. The results showed good mechanical properties, biocompatibility, and bone regeneration potential, indicating the effectiveness of this approach for bone defect repair.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2021)
Article
Engineering, Biomedical
Prerak Gupta, Gaurab Ranjan Chaudhuri, G. Janani, Manoj Agarwala, Debaki Ghosh, Samit K. Nandi, Biman B. Mandal
Summary: A novel cell-free bioresorbable small diameter silk TEVG system functionalized with decellularized human Wharton's jelly matrix has been developed and successfully implanted into rabbit jugular vein. The implanted TEVGs remained patent for two months and integrated with host tissue, demonstrating neo-tissue formation and constructive remodeling. The functionalized silk TEVGs showed increased host cell recruitment, supported superior graft remodeling, and mimicked the usefulness of cell seeding.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Lorena R. Lizarraga-Valderrama, Giulia Ronchi, Rinat Nigmatullin, Federica Fregnan, Pooja Basnett, Alexandra Paxinou, Stefano Geuna, Ipsita Roy
Summary: The study demonstrated that PHA-NGCs are highly promising in promoting peripheral nerve regeneration, showing comparable outcomes to autografts in functional evaluation and nerve morphology.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2021)
Review
Chemistry, Multidisciplinary
Kai Liu, Lesan Yan, Ruotao Li, Zhiming Song, Jianxun Ding, Bin Liu, Xuesi Chen
Summary: Nerve autograft is currently the preferred treatment for peripheral nerve defects but has limitations. Emerging nerve guide conduits (NGCs), especially those made through 3D printing, offer effective platforms for repairing peripheral nerve defects.
Article
Nanoscience & Nanotechnology
Junjie Shen, Jiayan Wang, Xuanzhe Liu, Yi Sun, Anlin Yin, Yimin Chai, Kuihua Zhang, Chunyang Wang, Xianyou Zheng
Summary: The study developed an in situ prevascularization strategy using 3D porous nerve guidance conduits for angiogenesis-mediated neural regeneration, showing significant neuroregeneration effects in vivo similar to traditional autografts.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biotechnology & Applied Microbiology
Xiang Zhang, Zhenxing Wang, Yu Shrike Zhang, Shujie Yan, Chuanyu Hou, Youping Gong, Jingjiang Qiu, Mo Chen, Qian Li
Summary: This study demonstrated that endothelialized tissue-engineered blood vessels cultured statically may experience endothelial cell shedding under arterial blood flow conditions, which was effectively addressed by regulating the flow field in a vascular chip platform. The adaptation of endothelial cells to artery-like microenvironment and improved cellular orientations were observed, providing a promising strategy for various applications in vascular engineering and drug analysis.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Polymer Science
Simzar Hosseinzadeh, Zeinab Zarei-Behjani, Mahboubeh Bohlouli, Arash Khojasteh, Nazanin Ghasemi, Nasim Salehi-Nik
Summary: Vascular regeneration relies on the proliferation and spreading of injured endothelial cells to control vascular wall thickness. Nanofibrous scaffolds prepared using various strategies, such as surface modification, co-electrospinning and blend-electrospinning, demonstrated high bioactivity in supporting endothelial cell attachment and proliferation. Incorporating somatotropin in the polyurethane fibers resulted in a sustained release pattern, enhancing endothelial cell proliferation for therapeutic purposes.
IRANIAN POLYMER JOURNAL
(2022)
Review
Immunology
Mireia Medrano-Bosch, Blanca Simon-Codina, Wladimiro Jimenez, Elazer R. Edelman, Pedro Melgar-Lesmes
Summary: Monocytes are circulating leukocytes that interact with endothelial cells to regulate inflammation, angiogenesis, and tissue remodeling under physiological and pathophysiological conditions. They transdifferentiate into macrophages and release various substances that induce vascular and tissue remodeling or propagate inflammatory responses. The interactions between infiltrating monocytes and endothelial cells contribute to pathophysiological conditions such as cardiovascular and chronic liver diseases.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Mascha Koenen, Michael A. Hill, Paul Cohen, James R. Sowers
Summary: Cardiovascular diseases, as the leading cause of death worldwide, are strongly associated with overweight and obesity, which contribute to vascular dysfunction and cardiovascular diseases. The abnormal remodeling of specific adipose tissue depots during obesity plays a critical role in the development of hypertension, endothelial dysfunction, and vascular stiffness, with fat distribution being a key determinant of the risk for cardiovascular diseases.
CIRCULATION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Tadeu Ravazi Piovesana, Lenize da Silva Rodrigues, Ana Livia de Carvalho Bovolato, Diego Noe Rodriguez-Sanchez, Jaqueline Carvalho Rinaldi, Nilton Jose Santos, Julia Calvi Mori, Pedro Luiz Toledo de Arruda Lourencao, Lynn Birch, Matheus Bertanha
Summary: The study evaluated the impact of implanting decellularized veins and autologous adipose-derived stem cells on tissue regeneration of the urinary bladder. Results showed that ASCs accelerated the regenerative process of the bladder and enhanced the maturity of mucosa and submucosa fibroblasts.
Article
Engineering, Biomedical
Jiesi Luo, Xiangyu Shi, Yuyao Lin, Yifan Yuan, Mehmet H. Kural, Juan Wang, Matthew W. Ellis, Christopher W. Anderson, Shang-Min Zhang, Muhammad Riaz, Laura E. Niklason, Yibing Qyang
Summary: The study successfully developed xenogeneic-free conditions for deriving functional endothelial cells from human induced pluripotent stem cells (XF-hiPSC-ECs), which exhibited similar marker expression and function to human primary ECs. Moreover, the XF-hiPSC-ECs responded to shear stress with typical cell alignment and gene expression, and were successfully used to endothelialize decellularized human vessels in a dynamic bioreactor system, advancing the potential clinical application of tissue engineered vascular grafts.
ACTA BIOMATERIALIA
(2021)
Review
Food Science & Technology
Mamali Das, Kasi Pandima Devi, Tarun Belwal, Hari Prasad Devkota, Devesh Tewari, Adeleh Sahebnasagh, Seyed Fazel Nabavi, Hamid Reza Khayat Kashani, Mahsa Rasekhian, Suowen Xu, Mehran Amirizadeh, Kiumarth Amini, Maciej Banach, Jianbo Xiao, Safieh Aghaabdollahian, Seyed Mohammad Nabavi
Summary: Vascular diseases are caused by abnormal endothelial response, resulting in atherosclerosis, hypercholesterolemia, etc. Polyphenols have antioxidative, anti-inflammatory, and anti-hypertensive properties, beneficial for preventing endothelial dysfunction.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Cell & Tissue Engineering
Nikhil B. Nayakawde, Ketaki Methe, Goditha U. Premaratne, Debashish Banerjee, Michael Olausson
Summary: The larynx is a complex organ consisting of various muscles, cartilages, mucosal membrane, and nerves, with larynx cancer being the most common type of head and neck cancer. Treatment options are limited for patients undergoing total or partial laryngectomy, but tissue-engineered organs have shown promise as an alternative. A novel decellularization method involving perfusion-agitation with detergents and ultrasonication was used to construct a pig larynx scaffold with preserved ECM proteins and angiogenic growth factors.
TISSUE ENGINEERING PART A
(2021)
Article
Immunology
Jiri Fronek, Libor Janousek, Jakub Kristek, Jaroslav Chlupac, Marek Pluta, Robert Novotny, Jana Maluskova, Michael Olausson
Summary: This study reported the first live birth after a deceased donor uterus transplant in Europe, demonstrating that nulliparous uteri may be a suitable source of uterine grafts for transplantation. Stenting appears to be a feasible treatment for vaginal anastomotic stenosis.
Article
Surgery
John M. Softeland, Jasmine Bagge, Arvind M. Padma, Anna Casselbrant, Changlian Zhu, Yafeng Wang, Mats Hellstrom, Michael Olausson, Mihai Oltean
Summary: The study validated the efficacy of luminal polyethylene glycol (PEG) in reducing damage to the intestinal epithelium during cold storage (CS) of intestinal grafts. Luminal PEG treatment significantly lowered mucosal injury, redistributed tight junction proteins earlier, and showed an emerging unfolding protein response after 24 hours of CS. Increased caspase activity was found in the control group, suggesting the potential of luminal PEG as a safe and effective intervention.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Article
Medicine, General & Internal
Jiri Fronek, Jakub Kristek, Jaroslav Chlupac, Libor Janousek, Michael Olausson
Summary: The results of the study demonstrate a high mid-term viability in uterus transplantation, with differences in outcomes between live donors and deceased donors. Live donor UTx has a higher success rate, and nulliparous donors should be considered for donation.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Orthopedics
Gennaro Selvaggi, Kristiina Manner, Augustinas Sakinis, Michael Olausson
Summary: Literature reports successful cases of penile transplantation for cis men, with no attempts for trans men. Modern surgical techniques for penis reconstruction may have limitations, making penis transplantation a potential alternative. The study confirmed the feasibility of using the internal pudendal artery for arterial anastomosis in transplantation procedures based on measurements of diameter and length.
JOURNAL OF PLASTIC SURGERY AND HAND SURGERY
(2022)
Article
Cell & Tissue Engineering
Debashish Banerjee, Nikhil B. Nayakawde, Deepti Antony, Meghshree Deshmukh, Sudip Ghosh, Carina Sihlbom, Evelin Berger, Uzair Ul Haq, Michael Olausson
Summary: Biological scaffolds prepared by decellularization and implanted using different routes were studied to understand the differential immune responses elicited. The use of sensitive protein and genomic tools provided valuable insights into the changes associated with chemical decellularization and postimplantation. This study contributes to the development of appropriate chemical strategies for biological scaffolds and the identification of implantable routes to overcome adverse immune reactions.
TISSUE ENGINEERING PART A
(2022)
Article
Immunology
Michael Olausson, Deepti Antony, Galina Travnikova, Martin Johansson, Nikhil B. Nayakawde, Debashish Banerjee, John Mackay Softeland, Goditha U. Premaratne
Summary: This study demonstrates that kidneys from extended uDCD, without aCPR/EC, can be salvaged using thrombolytic treatment to remove fibrin thrombi while preserving histology. These kidneys can then be transplanted with clinically acceptable early function.
Article
Biochemistry & Molecular Biology
Mihai Oltean, Jasmine Bagge, George Dindelegan, Diarmuid Kenny, Antonio Molinaro, Mats Hellstrom, Ola Nilsson, Carina Sihlbom, Anna Casselbrant, Marcela Davila, Michael Olausson
Summary: This study conducted a proteome-wide analysis in a mouse model of intestinal transplantation and identified proteome changes associated with rejection, potentially serving as biomarkers for intestinal rejection.
Article
Obstetrics & Gynecology
Mats Brannstrom, Pernilla Dahm-Kahler, Niclas Kvarnstrom, Anders Enskog, Jan I. Olofsson, Michael Olausson, Johan Molne, Randa Akouri, Stina Jarvholm, Lars Nilsson, Lennart Stigson, Henrik Hagberg, Hans Bokstrom
Summary: The first completed study of uterus transplantation (UTx) demonstrates that it is an effective treatment for infertility, resulting in the birth of healthy children and having only minor psychological and medical long-term effects on donors and recipients.
FERTILITY AND STERILITY
(2022)
Article
Biochemistry & Molecular Biology
Vijay Kumar Kuna, Andre Lundgren, Luis Oliveros Anerillas, Peyman Kelk, Maria Brohlin, Mikael Wiberg, Paul J. Kingham, Ludmila N. Novikova, Gustav Andersson, Lev N. Novikov
Summary: In this study, peripheral nerve-derived hydrogels were prepared using different decellularization methods, and their efficacy in nerve regeneration was evaluated. The hydrogel prepared using sodium deoxycholate showed the best preservation of extracellular matrix and support for nerve regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Michael Olausson, Deepti Antony, Martin Johansson, Galina Travnikova, Nikhil B. Nayakawde, Debashish Banerjee, John Mackay Softeland, Damiano Ognissanti, Moa Andresen Bergstrom, Ola Hammarsten, Goditha U. Premaratne
Summary: In this study, a novel thrombolytic technique was used to salvage kidneys from porcine donors with uncontrolled circulatory deaths. The kidneys were then reconditioned ex vivo and transplanted. The results showed that after 3 months, the transplanted kidneys had similar function and histology compared to kidneys from living donors.
Article
Physiology
Jakub Kristek, Eva Sticova, Jaroslav Chlupac, Helena Cermakova, Jana Maluskova, Libor Janousek, Michael Olausson, Jiri Fronek
Summary: This paper presents an interim analysis of a trial focusing on graft thrombosis in uterine transplantation. The study analyzed 10 cases and found that early thrombosis occurred in 20% of recipients, leading to graft loss in all cases despite attempts at thrombectomy. The study highlights the importance of prevention, thorough evaluation of donors, and close monitoring in the postoperative period.
PHYSIOLOGICAL RESEARCH
(2022)
Meeting Abstract
Surgery
Michael Olausson, Galyna Travnikova, Goditha Premaratne, Debashish Banerjee, Martin Johansson, Nikhil Nayakawde, John Softeland, Deepti Antony
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Meeting Abstract
Surgery
Michael Olausson, Galina Travnikova, Goditha Premaratne, Debashish Banerjee, Nikhil Nayakawde, Martin Johansson, John Softeland, Deepti Antony
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Gastroenterology & Hepatology
Zhiwen Liu, Vijay Kumar Kuna, Bo Xu, Suchitra Sumitran-Holgersson
Summary: This study demonstrates that Wnt3a and Wnt5a may enhance the proliferation and migration of hFLPC by regulating key target genes, potentially playing an important role in liver regeneration processes. Incorporating recombinant human Wnt3a and Wnt5a in regenerative strategies using liver stem/progenitor cells could potentially improve the process of liver regeneration.
TRANSLATIONAL GASTROENTEROLOGY AND HEPATOLOGY
(2021)
