Article
Developmental Biology
Mostafa Aakhte, Hans-Arno J. Mueller
Summary: Light-sheet or selective plane illumination microscopy (SPIM) is suitable for in toto imaging of living specimens at high temporal-spatial resolution, but faces limitations due to light scattering in opaque specimens. The Multiview tiling SPIM (MT-SPIM) combines the strengths of Multi-view SPIM (M-SPIM) and a confined, multi-tiled light sheet, providing high-resolution, robust, and rotation-free imaging of living specimens. It improves axial resolution by a factor of two, enhancing automated segmentation of cellular structures.
Article
Biochemical Research Methods
Jeonghwan Son, Biagio Mandracchia, Aaron D. Silva Trenkle, Gabriel A. Kwong, Shu Jia
Summary: PLSOM is a portable 3D fluorescence cytometric imaging technique that offers high resolution, high throughput, and compatibility with commonly adopted microfluidic chips. It can be widely used for 3D flow cytometric investigations.
Article
Optics
N. R. Subedi, S. Yaraghi, P. S. Jung, G. Kukal, A. G. McDonald, D. N. Christodoulides, A. E. Vasdekis
Summary: Research shows that digitally scanned Airy beams can improve the throughput rates in Raman imaging by an order of magnitude compared to traditional diffraction-limited beams, achieving high contrast and 1 micron axial resolution for three-dimensional imaging of microparticles. This method also achieves orders of magnitude lower irradiation density than coherent Raman imaging schemes, with faster speed and lower phototoxicity.
Article
Biology
Anna Schueth, Sven Hildebrand, Iryna Samarska, Shubharthi Sengupta, Annemarie Kiessling, Andreas Herrler, Axel zur Hausen, Michael Capalbo, Alard Roebroeck
Summary: This study demonstrates the feasibility of 3D imaging of large human tissue samples using light-sheet imaging technology, providing high-resolution images for both brain and prostate cancer samples.
COMMUNICATIONS BIOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Yuan Luo, Ming Lun Tseng, Sunil Vyas, Ting-Yu Hsieh, Jui-Ching Wu, Shang-Yang Chen, Hsiao-Fang Peng, Vin-Cent Su, Tzu-Ting Huang, Hsin Yu Kuo, Cheng Hung Chu, Mu Ku Chen, Jia-Wern Chen, Yu-Chun Chen, Kuang-Yuh Huang, Chieh-Hsiung Kuan, Xu Shi, Hiroaki Misawa, Din Ping Tsai
Summary: Light-sheet fluorescent microscopy (LSFM) is the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. This study demonstrates the successful integration of a nanophotonic meta-lens as the illumination component for LSFM, addressing the challenges of high image resolution and optical sectioning. With the meta-lens, the LSFM system complexity is significantly reduced, enabling multicolor fluorescent imaging of live biological specimens with cellular resolution.
Article
Environmental Sciences
Dylan L. Windell, Sulayman Mourabit, Julian Moger, Stewart F. Owen, Matthew J. Winter, Charles R. Tyler
Summary: Gold nanoparticles (AuNPs) have different fates and behaviors in biological systems depending on their size and surface coating. In this study, we investigated the effects of AuNP size and surface chemistry on their bioavailability, tissue distribution, and potential toxicity in zebrafish. Our results showed that the accumulation of AuNPs in zebrafish organs was related to particle size, and surface modifications enhanced particle accumulation in the pronephric tubules. The presence of AuNPs did not result in measurable toxicity in terms of organ function or cellular oxidative stress for short term exposures.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biochemical Research Methods
Kevin M. Dean, Tonmoy Chakraborty, Stephan Daetwyler, Jinlong Lin, Gerard Garrelts, Ons M'Saad, Hannahmariam T. Mekbib, Fabian F. Voigt, Martina Schaettin, Esther T. Stoeckli, Fritjof Helmchen, Joerg Bewersdorf, Reto Fiolka
Summary: The protocol provides detailed instructions for assembling and operating a versatile variant of light-sheet fluorescence microscopy called axially swept light-sheet microscopy (ASLM), which offers an unparalleled combination of field of view, optical resolution, and optical sectioning. It includes information on the working principle, applications, practical tips, part lists, schematics, and software for instrument control of ASLM.
Article
Biochemical Research Methods
Petra Paie, Gianmaria Calisesi, Alessia Candeo, Andrea Comi, Federico Sala, Francesco Ceccarelli, Ada De Luigi, Pietro Veglianese, Korbinian Muhlberger, Michael Fokine, Gianluca Valentini, Roberto Osellame, Mark Neil, Andrea Bassi, Francesca Bragheri
Summary: Heterogeneity investigation at the single-cell level is crucial in clinical research. The combination of light sheet fluorescence microscopy and structured illumination microscopy in an optofluidic platform enables high throughput super-resolution imaging, allowing comprehensive evaluation of cellular heterogeneity at high resolution.
Article
Biochemical Research Methods
Tingting Zhu, Jun Nie, Tingting Yu, Dan Zhu, Yanyi Huang, Zaozao Chen, Zhongze Gu, Jiang Tang, Dongyu LI, Peng Fei
Summary: Light sheet microscopy combined with a microchip is an emerging tool in biomedical research that improves efficiency. However, noticeable aberrations induced by the complex refractive indices in the chip limit microchip-enhanced light-sheet microscopy. This study introduces a droplet microchip with a polymer index matched to water, enabling large-scale culture of 3D spheroids and time-lapse imaging with high resolution and throughput.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Developmental Biology
Alba Vieites-Prado, Nicolas Renier
Summary: Tissue clearing technology enhances the transparency of late developmental stages and enables deep imaging in fixed organisms. Successful implementation of these techniques requires a good understanding of sample processing, imaging, and the possibilities offered by image analysis.
Article
Optics
Dongli Xu, Jun B. Ding, Leilei Peng
Summary: Two-photon light-sheet fluorescence microscopy allows high-resolution imaging of neural activity in brain tissue at a high frame rate. The traditional light-sheet microscopy technique has limitations in volumetric imaging speed, but with the introduction of depth random-access light-sheet microscopy, rapid switching of scanning depth for light-sheet imaging is possible, resulting in faster imaging and higher refreshing rates.
Article
Multidisciplinary Sciences
Eliana Battistella, Jan Schniete, Katrina Wesencraft, Juan F. Quintana, Gail McConnell
Summary: By using light-sheet mesoscopy, we have developed a high-speed imaging technique that allows rapid imaging of large biological tissue specimens. This opens up new possibilities for high-content, higher-throughput optical bioimaging at the mesoscale.
Article
Cell Biology
Simona Machacova, Helena Chmelova, Anna Vavrova, Zbynek Kozmik, Iryna Kozmikova
Summary: Cephalochordates serve as representatives of the most basally divergent group of the chordate phylum, offering crucial insights into vertebrate evolution. In evolutionary developmental research, the combination of light sheet microscopy and tissue clearing methods allows for visualization of anatomical structures and individual cells of amphioxus in 3D space.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Cynthia Richard, Erick J. Vargas-Ordaz, Victor J. Cadarso, Adrian Neild
Summary: Having a system that can capture high-resolution, quantitative, subcellular information of live cells without causing cell damage is crucial in various research fields. Optofluidic light-sheet fluorescent microscopy (LSFM) has emerged as a powerful tool that offers a low photo-toxic imaging method using microfluidics. In this study, LSFM benefits were combined with an acoustic single cell prison to enable precise single cell manipulation and 3D multi-imaging in a semi-automated manner. By trapping individual cells using standing surface acoustic waves (SSAWs), planar and cross-sectional images of the cells were obtained. The results were further processed to reconstruct 3D volumetric images and compare imaging outcomes between rounds.
MICROFLUIDICS AND NANOFLUIDICS
(2023)
Review
Neurosciences
Hannah C. Bennett, Yongsoo Kim
Summary: This article highlights advances in high-resolution 3D mapping methods for studying the cerebrovascular network and neurovascular unit (NVU) in the mouse brain. The use of block-face imaging and light sheet fluorescent microscopy has enabled researchers to examine the anatomical arrangement of the cerebrovascular network and gain biological insights. These imaging techniques have the potential to advance our understanding of brain disorders and overall brain health.
Article
Oncology
Aurelie Gomes, Matthieu Defaux, Riwanon Michel Lemee, Valerie Lobjois, Bernard Ducommun
Article
Oncology
Fabien Gava, Lise Rigal, Odile Mondesert, Elise Pesce, Bernard Ducommun, Valerie Lobjois
Article
Biology
T. Michel, J. Fehrenbach, V Lobjois, J. Laurent, A. Gomes, T. Colin, C. Poignard
JOURNAL OF THEORETICAL BIOLOGY
(2018)
Article
Cell Biology
David Bernard, Odile Mondesert, Aurelie Gomes, Yves Duthen, Valerie Lobjois, Sylvain Cussat-Blanc, Bernard Ducommun
Article
Multidisciplinary Sciences
Laurene Aoun, Stanislas Larnier, Pierre Weiss, Martine Cazales, Ariane Herbulot, Bernard Ducommun, Christophe Vieu, Valerie Lobjois
Article
Multidisciplinary Sciences
Ludivine Guillaume, Lise Rigal, Jerome Fehrenbach, Childerick Severac, Bernard Ducommun, Valerie Lobjois
SCIENTIFIC REPORTS
(2019)
Article
Cell Biology
Julia Bonnet, Lise Rigal, Odile Mondesert, Renaud Morin, Gaelle Corsaut, Mathieu Vigneau, Bernard Ducommun, Valerie Lobjois
Summary: The study demonstrated that mitotic arrest induced by microtubule-targeting anticancer drugs can prevent cancer cell clustering, potentially reducing the metastatic potential of circulating tumor cells.
Article
Oncology
Fabien Gava, Julie Pignolet, Sebastien Dejean, Odile Mondesert, Renaud Morin, Joseph Agossa, Bernard Ducommun, Valerie Lobjois
Summary: This study identified genes significantly associated with tumor cell aggregation by analyzing the relationship between aggregation quantitative characterization of 25 cancer cell lines and their expression data. Interestingly, these genes were found to be strongly correlated with the transcriptional signature induced by HDAC inhibitors treatment, and experimental evidence showed that two HDAC inhibitors could inhibit tumor cell cluster formation in vitro. These findings open up new therapeutic perspectives for preventing metastasis formation.
Article
Developmental Biology
Angie Molina, Frederic Bonnet, Julie Pignolet, Valerie Lobjois, Sophie Bel-Vialar, Jacques Gautrais, Fabienne Pituello, Eric Agius
Summary: Although the lengthening of cell cycle and G1 phase is a common phenomenon during tissue maturation, the underlying mechanism is still unclear. This study developed a time-lapse imaging strategy to measure the four cell cycle phases in single chick neural progenitor cells, and found that neural progenitors exhibit wide heterogeneity in cell cycle length. The study also identified a mechanism that contributes to the lengthening of G1 phase.
Article
Oncology
Arnaud Carrier, Cecile Desjobert, Valerie Lobjois, Lise Rigal, Florence Busato, Jorg Tost, Miquel Ensenyat-Mendez, Diego M. Marzese, Anne Pradines, Gilles Favre, Laurence Lamant, Luisa Lanfrancone, Chantal Etievant, Paola B. Arimondo, Joelle Riond
Summary: Protocadherin proteins play an important role in cell adhesion and signaling pathways. The study found that the PCDHB15 gene's specific exon is hypermethylated in melanoma, leading to its silencing. This silencing contributes to the aggressiveness of melanoma.
CLINICAL EPIGENETICS
(2022)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
