4.0 Review

Introduction to super-resolution microscopy

Journal

MICROSCOPY
Volume 63, Issue 3, Pages 177-192

Publisher

OXFORD UNIV PRESS
DOI: 10.1093/jmicro/dfu007

Keywords

Super-resolution microscopy; STED; PALM; STORM; SIM; SAX

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Funding

  1. Next Generation World-Leading Researchers (NEXT Program) of the Japan Society for the Promotion of Science (JSPS)
  2. Network Joint Research Center for Materials and Devices

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In this review, we introduce the principles of spatial resolution improvement in super-resolution microscopies that were recently developed. These super-resolution techniques utilize the interaction of light and fluorescent probes in order to break the diffraction barrier that limits spatial resolution. The imaging property of each super-resolution technique is also compared with the corresponding conventional one. Typical applications of the super-resolution techniques in biological research are also introduced.

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