Article
Biochemistry & Molecular Biology
Lingxia Lyu, Zhoubin Li, Zuoshi Wen, Yongchun He, Xuliang Wang, Liujun Jiang, Xuhao Zhou, Chengchen Huang, Yutao Wu, Ting Chen, Xiaogang Guo
Summary: The down-regulation of lncRNA Malat1 in vascular diseases promotes differentiation of Sca1(+) stem/progenitor cells into smooth muscle cells, leading to excessive accumulation and vessel stenosis. Malat1 regulates smooth muscle cell regeneration through the miR125a-5p/Stat3 signaling pathway. These findings highlight the critical role of Sca1(+) stem/progenitor cells in vascular remodeling and suggest Malat1 as a potential biomarker or therapeutic target for vascular diseases.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Cell & Tissue Engineering
Nan Hu, Linqing Zou, Cheng Wang, Guoqi Song
Summary: RUNX1T1 plays important roles in acute myeloid leukemia, neuronal development, intestinal development, adipogenesis, angiogenesis, and carcinogenesis, affecting cell fate through transcriptional regulation and signaling pathways.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Multidisciplinary Sciences
SeungHye Han, Minho Lee, Youngjin Shin, Regina Giovanni, Ram P. Chakrabarty, Mariana M. Herrerias, Laura A. Dada, Annette S. Flozak, Paul A. Reyfman, Basil Khuder, Colleen R. Reczek, Lin Gao, Jose Lopez-Barneo, Cara J. Gottardi, G. R. Scott Budinger, Navdeep S. Chandel
Summary: Alveolar epithelial type 1 (AT1) cells are necessary for gas exchange, while alveolar epithelial type 2 (AT2) cells play a role in lung development and repair. In this study, deleting a specific subunit of the mitochondrial electron transport chain complex I in lung epithelial cells led to abnormal alveolar development and death. Restoring NAD(+) regeneration was able to correct the developmental defects and prevent lethality. These findings highlight the importance of mitochondrial complex I-dependent NAD(+) regeneration in directing cell fate during lung development.
Article
Biochemical Research Methods
Marius Lange, Volker Bergen, Michal Klein, Manu Setty, Bernhard Reuter, Mostafa Bakhti, Heiko Lickert, Meshal Ansari, Janine Schniering, Herbert B. Schiller, Dana Pe'er, Fabian J. Theis
Summary: Computational trajectory inference is a method to reconstruct cell state dynamics from single-cell RNA sequencing experiments, but it requires prior knowledge of the direction of biological processes. CellRank is a new approach that allows single-cell fate mapping in scenarios where the direction is unknown, such as regeneration, reprogramming, and disease.
Editorial Material
Immunology
Joshua J. C. McGrath, Patrick C. Wilson
Summary: Molecular fate-mapping reveals the complex dynamics of antibodies resulting from sequential immunization, which hampers the study of concepts like 'original antigenic sin'. Additionally, it demonstrates that boosted antibodies exhibit an addiction to primary clones and suppress the formation of new clones in an OAS-like manner, with the degree of suppression inversely related to the antigenic distance of the booster.
Article
Multidisciplinary Sciences
Arien Schiepers, Marije F. L. van't Wout, Allison J. J. Greaney, Trinity Zang, Hiromi Muramatsu, Paulo J. C. Lin, Ying K. K. Tam, Luka Mesin, Tyler N. N. Starr, Paul D. D. Bieniasz, Norbert Pardi, Jesse D. D. Bloom, Gabriel D. D. Victora
Summary: The effectiveness of serum antibodies in providing protection is determined by the interactions between antigen-specific B cell clones with different affinities and specificities. These interactions lead to phenomena such as original antigenic sin (OAS), where the immune system relies on the initial cohort of B cells activated by an antigenic stimulus instead of inducing new responses. This suppression of new antibody responses may hinder vaccination against rapidly evolving viruses, highlighting the importance of understanding and addressing OAS for the design of effective vaccines.
Article
Multidisciplinary Sciences
Xuan Zheng, Max A. Betjes, Pascal Ender, Yvonne J. Goos, Guizela Huelsz-Prince, Hans Clevers, Jeroen S. van Zon, Sander J. Tans
Summary: This study developed a new tool called TypeTracker, which identifies cell fates by AI-enabled cell tracking and propagates end point fates back along the branched lineage trees. The research found that cells commit to their new type early, when still deep inside the crypt, contrary to the conventional belief that cells differentiate as they move up the crypt-villus axis. This study raises new questions about the underlying commitment and sorting mechanisms.
Article
Multidisciplinary Sciences
Sabrina Summer, Eva Rossmanith, Markus Pasztorek, Constantin Fiedler, Marion Groeger, Sabine Rauscher, Viktoria Weber, Michael B. Fischer
Summary: Mesenchymal stem cells play a crucial role in supporting endothelial cells during tissue regeneration and new vessel formation. By co-culturing these cells in a specific microenvironment, complex vascular networks can be formed. Under dynamic flow conditions, mesenchymal stem cells can bind to damaged endothelial cells, potentially aiding in the healing process.
Article
Cardiac & Cardiovascular Systems
Joseph M. Miano, Edward A. Fisher, Mark W. Majesky
Summary: Studying the phenotypic modulation of vascular smooth muscle cells is crucial for understanding the pathogenesis of atherosclerosis and developing new therapies. Recent technological and conceptual advances shed light on the multifunctionality and plasticity of VSMCs.
Review
Cell Biology
Sadie VanHorn, Samantha A. Morris
Summary: Lineage tracing and fate mapping are two disciplines for tracking cells and their progeny, with lineage tracing focusing on identifying progeny and fate maps showing embryo development. Recent genomic technologies have enabled high-resolution reconstruction of lineage relationships. Fate maps, however, offer spatial information often lost in lineage reconstruction.
DEVELOPMENTAL CELL
(2021)
Article
Cell Biology
Lemonia Chatzeli, Ignacio Bordeu, Seungmin Han, Sara Bisetto, Zahra Waheed, Bon-Kyoung Koo, Maria P. Alcolea, Benjamin D. Simons
Summary: The development of the mouse salivary gland involves a process of branching morphogenesis, in which progenitor cells transition from a multipotent state to unipotent states via two distinct bipotent states. Notch signaling and Kras activation play important roles in promoting specific lineages. These findings provide insights into the mechanisms underlying lineage segregation and ductal patterning.
DEVELOPMENTAL CELL
(2023)
Review
Medicine, General & Internal
Serena Barachini, Sandra Ghelardoni, Rosalinda Madonna
Summary: Vascular progenitor cells are activated to repair and form a neointima following various vascular damages. They not only play a role in resolving vascular lesions, but also contribute to neovascularization and angiogenesis associated with cancer. This review discusses the biology and pathophysiology of vascular progenitor cells, including their origins, stimuli, and activated pathways. It focuses on their involvement in tumor-induced vascular injury and their implications in promoting tumor angiogenesis during cancer proliferation and migration.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Mina N. F. Morcos, Congxin Li, Clara M. Munz, Alessandro Greco, Nicole Dressel, Susanne Reinhardt, Katrin Sameith, Andreas Dahl, Nils B. Becker, Axel Roers, Thomas Hoefer, Alexander Gerbaulet
Summary: In this study, the authors use single-cell RNA-seq, computational integration of non-perturbative approaches for fate-mapping, and mitotic tracking to study lineage decisions in native hematopoiesis and identify megakaryocyte progenitors that directly link HSCs to megakaryocytes.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Wendy Xueyi Wang, Julie L. Lefebvre
Summary: Understanding the diversification of neuronal circuits in the cerebellum is crucial, and this study provides novel insights by combining genetic fate-mapping, morphological profiling, and molecular labeling techniques. The authors identify diverse types of molecular layer interneurons (MLIs) that arise from a common progenitor population. They demonstrate that MLIs can be categorized into two distinct morphological types with extensive within-class heterogeneity. Moreover, they show that subtype divergence can be resolved based on axonal morphogenesis before marker gene expression. This study highlights the importance of single-cell methods in quantifying neuronal diversification and sheds light on the dissociation between morphology and transcriptomics in understanding neuronal diversity.
NATURE COMMUNICATIONS
(2022)
Review
Cell Biology
Sierra R. Scamfer, Mark D. Lee, Keren Hilgendorf
Summary: This review summarizes the role of the primary cilium in regulating the fate of adipocyte progenitor cells and highlights its importance in energy storage and adipose tissue function.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)