Article
Plant Sciences
Avinash Sanap, Kalpana Joshi, Tejas Shah, Girish Tillu, Ramesh Bhonde
Summary: The study showed that Piper longum L. fruit extract significantly increased proliferation of WJMSCs and enhanced Ca2+ accumulation and matrix mineralization, along with promoting alkaline phosphatase (ALP) activity and the expression of key gene mRNAs. This suggests that the fruit extract can enhance osteogenic differentiation of WJMSCs, potentially serving as an anti-osteoporotic agent.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Cell & Tissue Engineering
Changzhen Wang, Hongmei Ning, Jiao Gao, Teng Xue, Ming Zhao, Xiaoxia Jiang, Xiaoming Zhu, Ximin Guo, Hong Li, Xiaoyan Wang
Summary: This study investigated the impact of hematopoietic cells on MSCs and found that partial deletion of the Pten gene in hematopoietic cells can lead to abnormal differentiation of MSCs. There is a reciprocal relationship between hematopoietic cells and MSCs, with an important role in bone development.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Juan C. Henao, Adriana Grismaldo, Alfonso Barreto, Viviana M. Rodriguez-Pardo, Claudia Camila Mejia-Cruz, Efrain Leal-Garcia, Rafael Perez-Nunez, Patricio Rojas, Ramon Latorre, Ingrid Carvacho, Yolima P. Torres
Summary: The study demonstrates that TRPM8 channels are expressed in human bone marrow MSCs and are involved in osteogenic differentiation, with agonists promoting osteogenic differentiation and antagonists leading to a decrease in osteogenic differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Aleksandra Kostina, Arseniy Lobov, Daria Semenova, Artem Kiselev, Polina Klausen, Anna Malashicheva
Summary: Despite significant progress in bone tissue regeneration, the early mechanisms of osteogenic differentiation remain poorly understood. Various cells can undergo osteogenic transformation, with Notch signaling playing a key role in this process. Research demonstrates differences in the ability of different cell types to undergo Notch-dependent osteogenic differentiation.
Review
Pharmacology & Pharmacy
Jinli Zhang, Zhihe Liu, Yang Luo, Xiaojian Li, Guowei Huang, Huan Chen, Aiguo Li, Shengnan Qin
Summary: Flavonoids have the potential to regulate the osteogenic differentiation of mesenchymal stem cells (MSCs), making them a promising therapeutic application in bone disorders and bone tissue engineered constructs.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Enrico C. Torre, Mesude Bicer, Graeme S. Cottrell, Darius Widera, Francesco Tamagnini
Summary: Adipose-derived mesenchymal stromal cells (ASCs) have the potential to differentiate into various cell types and exhibit spontaneous Ca2+ oscillations. The effects of differentiation treatments on ASCs' Ca2+ dynamics, in terms of oscillation fraction and frequency, were examined in this study. Both adipogenic and osteogenic differentiation resulted in reduced Ca2+ dynamics, with adipogenic differentiation showing a more pronounced effect. Changes in Ca2+ oscillations were observed as early as 7 days in vitro (DIV). Further research is needed to understand the molecular and mechanistic correlates of these changes for the development of interventions in regenerative medicine.
Review
Biochemistry & Molecular Biology
Chiara Mazziotta, Carmen Lanzillotti, Maria Rosa Iaquinta, Francesca Taraballi, Elena Torreggiani, John Charles Rotondo, Lucia Oton-Gonzalez, Elisa Mazzoni, Francesca Frontini, Ilaria Bononi, Monica De Mattei, Mauro Tognon, Fernanda Martini
Summary: MSCs are important source for bone regeneration and play a crucial role in osteogenic differentiation. MiRNAs regulate gene expression in osteogenic differentiation of MSCs and are involved in controlling important signaling pathways such as TGF-beta/BMP and Wnt/beta-catenin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Chanchao Lorthongpanich, Thanapon Charoenwongpaiboon, Prapasri Supakun, Methus Klaewkla, Pakpoom Kheolamai, Surapol Issaragrisil
Summary: The study showed that fisetin can inhibit proliferation, migration, and osteogenic differentiation of human MSCs and SaOs-2 cells by reducing YAP activity, leading to downregulation of osteogenic genes and upregulation of fibroblast genes. Molecular docking and simulations suggest that fisetin may disrupt protein-protein interactions by occupying the TEAD pocket, indicating its potential role in regulating human osteogenesis.
Review
Polymer Science
Aiah A. El-Rashidy, Sara El Moshy, Israa Ahmed Radwan, Dina Rady, Marwa M. S. Abbass, Christof E. Doerfer, Karim M. Fawzy El-Sayed
Summary: The stiffness of the extracellular matrix plays a regulatory role in directing the osteogenic differentiation of MSCs, with MSCs sensing and responding to their ECM to undergo differentiation. Customizing scaffold materials to mimic cells' natural environment is crucial in the tissue engineering process.
Article
Chemistry, Physical
Jing Liu, Yaya Cheng, Haoyu Wang, Dingyi Yang, Cunshun Liu, Weixin Dou, Xue Jiang, Hongzhang Deng, Rusen Yang
Summary: Researchers developed a composite piezoelectric fibrous membrane with titanium dioxide nanoparticles and PVDF for cellular osteogenic differentiation. The membrane significantly promoted the expression of osteocalcin gene and cell adhesion and proliferation through electromechanical stimulation. The proposed mechanism provides guidance for designing electroactive materials to promote osteogenic differentiation and bone regeneration.
Article
Biochemistry & Molecular Biology
Jiang Liu, Xin-Tong Yao, Xiao-Lei Feng, Xi-Zhuang Bai
Summary: In recent years, there has been increasing attention on bone loss related diseases, such as osteoporosis and osteonecrosis of the femoral head, which exhibit symptoms of insufficient bone mass. Mesenchymal stem cells (MSCs) hold potential as a new solution for bone disease, as they can be induced to differentiate into osteoblasts. This study investigates the mechanism by which BMP2 drives MSCs to differentiate into osteoblasts through ACKR3/p38/MAPK signaling. The findings suggest that ACKR3 could be a novel therapeutic target for bone-associated diseases and bone-tissue engineering.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Toxicology
AtLee T. D. Watson, Aldo Carmona Baez, Dereje Jima, David Reif, Jun Ding, Reade Roberts, Seth W. Kullman
Summary: This study aims to investigate the regulatory mechanisms of osteoblast differentiation through global transcriptomic analysis of human bone-derived mesenchymal stem cells (hBMSCs) exposed to TCDD. The results show that TCDD exposure attenuates osteoblast differentiation of hBMSCs and leads to altered gene expression.
TOXICOLOGICAL SCIENCES
(2023)
Article
Cell & Tissue Engineering
Arseniy Lobov, Polina Kuchur, Aigul Khizhina, Anastasia Kotova, Andrei Ivashkin, Daria Kostina, Polina Klausen, Evgeniia Khokhlova, Egor Repkin, Kseniia Postnikova, Daria Perepletchikova, Evgeny Denisov, Tatiana Gerashchenko, Rashid Tikhilov, Svetlana Bozhkova, Andrey Sereda, Vitaliy Karelkin, Natella Enukashvily, Anna Malashicheva
Summary: In this study, we conducted a comparative analysis of mesenchymal cell primary cultures from various sources and found differences in their ability to undergo osteogenic differentiation and produce extracellular matrix. Based on the analysis of secretome, proteome, and transcriptome, we classified them into dental stem cells, mesenchymal stem cells, and fetal stem cells. Although they have differences, mesenchymal cells from all sources secreted core bone extracellular matrix-associated proteins. These findings shed light on the distinctive characteristics of mesenchymal stem cells and provide insights into their potential applications in regenerative medicine and the inherent diversity of mesenchymal cells in vivo.
Article
Cell & Tissue Engineering
Hongwei Gao, Hui Dong, Jiachun Zheng, Xufeng Jiang, Mingzhi Gong, Le Hu, Jinshan He, Yongxiang Wang
Summary: The study identified LINC01119 as a negative regulator of osteogenic differentiation in MSCs, with its knockdown promoting calcification and osteogenic differentiation. It was revealed that LINC01119 negatively regulates osteogenesis through the Wnt pathway by targeting FZD4, with transcription factor EBF3 directly binding to LINC01119's promoter region.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Genetics & Heredity
Duojiao Chen, Sheng Liu, Xiaona Chu, Jill Reiter, Hongyu Gao, Patrick Mcguire, Xuhong Yu, Xiaoling Xuei, Yichen Liu, Jun Wan, Fang Fang, Yunlong Liu, Yue Wang
Summary: In this study, single-cell multiomic analysis was used to profile the transcriptome and epigenome of mesenchymal stem cells (MSC) from four healthy donors. The analysis revealed distinct chromatin accessible regulatory elements and differentiation potential among the MSC from different donors. The study also explored the enrichment of genetic variants associated with musculoskeletal diseases in the chromatin accessible regions, providing insights into the regulatory mechanisms of gene expression during MSC differentiation.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)