Review
Endocrinology & Metabolism
Alberto Posabella, Andrea B. Alber, Hendrik J. Undeutsch, Raoul A. Droeser, Anthony N. Hollenberg, Laertis Ikonomou, Darrell N. Kotton
Summary: Stem cell-based therapies hold potential for reconstituting organ function, particularly in addressing hypothyroidism. Research has shown promise in engineering thyroid follicular epithelial cells to potentially cure the disease, but technical and biological challenges need to be overcome to achieve long-term cure through cell- and/or gene-based therapies.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Matin Chehelgerdi, Fereshteh Behdarvand Dehkordi, Mohammad Chehelgerdi, Hamidreza Kabiri, Hosein Salehian-Dehkordi, Mohammad Abdolvand, Sharareh Salmanizadeh, Mohsen Rashidi, Anoosha Niazmand, Saba Ahmadi, Sara Feizbakhshan, Saber Kabiri, Nasimeh Vatandoost, Tayebeh Ranjbarnejad
Summary: The emergence of iPSCs has revolutionized stem cell research and opened up new avenues for cancer treatment. iPSCs provide a unique platform for investigating the origin of cancer and can be used for personalized treatment and drug testing. This review article discusses the science behind iPSCs, the limitations and challenges in iPSC-based cancer therapy, ethical and social implications, and a comparative analysis with other stem cell types for cancer treatment. It also explores the applications of iPSCs in tumorigenesis and the future of iPSC research in this field.
Article
Cell Biology
Glyn Nigel Stacey, Jie Hao
Summary: In recent years, significant progress has been made in China in establishing hPSCs for the manufacture of cell therapies. The National Stem Cell Resource Center and its partner organizations have over 600 hPSC lines formally recorded, with 47 of them also registered for European Commission funded research projects. This national and international coordination aims to promote the translation of Chinese hPSC-based products into clinical use according to established standards.
CELL PROLIFERATION
(2022)
Review
Biochemistry & Molecular Biology
Agnieszka Fus-Kujawa, Barbara Mendrek, Anna Trybus, Karolina Bajdak-Rusinek, Karolina L. Stepien, Aleksander L. Sieron
Summary: Induced pluripotent stem cells (iPSCs) show potential in gene therapy and disease modeling. To safely apply this technology in clinical settings, optimal reprogramming factors and vectors must be considered to reduce the risk of insertional tumorigenesis.
Article
Cell & Tissue Engineering
Toru Kondo
Summary: The study demonstrates that the DHODH inhibitor BRQ induces differentiation in mouse PSCs, preventing teratoma formation, while showing low toxicity to normal cells. This suggests that DHODH inhibitors like BRQ could be crucial for successful PSC-based therapy.
Review
Biochemistry & Molecular Biology
Riccardo Ossanna, Sheila Veronese, Lindsey Alejandra Quintero Sierra, Anita Conti, Giamaica Conti, Andrea Sbarbati
Summary: Cell-based therapy has the potential to restore lost cells and promote regeneration in various human disorders. Stem cells, particularly pluripotent stem cells and recently discovered Muse cells, hold great promise for clinical medicine. Muse cells are pluripotent cells found in adult human mesenchymal tissues, offering advantages such as autologous treatment and avoidance of rejection issues in transplants. They have shown promising results in preclinical studies and could expand therapeutic applications in regenerative medicine.
Article
Biochemistry & Molecular Biology
Summer A. Helmi, Leili Rohani, Ahmed R. Zaher, Youssry M. El Hawary, Derrick E. Rancourt
Summary: Bone healing is a well-organized process regulated by multiple factors. The Notch signaling pathway plays a significant role in controlling bone mesenchymal stem cell differentiation. Inhibiting the Notch pathway can greatly enhance the differentiation of both embryonic and induced pluripotent stem cells, potentially improving overall healing outcomes. Stem cell transplantations are prominent candidates for treating critical-sized bone defects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Tzu-Cheng Sung, Yi-Peng Jiang, Jhe-Yu Hsu, Qing-Dong Ling, Hao Chen, Suresh S. Kumar, Yung Chang, Shih-Tien Hsu, Qingsong Ye, Akon Higuchi
Summary: This study successfully generated universal hiPSCs without gene editing and evaluated their resistance to immune reactions, demonstrating that the cells maintained stable characteristics even after continuous passages.
CELL PROLIFERATION
(2021)
Review
Pharmacology & Pharmacy
Lu Zhang, Xiao-Jing-Nan Ma, Yuan-Yuan Fei, Heng-Tong Han, Jun Xu, Lu Cheng, Xun Li
Summary: The liver has the ability to repair itself, but pathological changes can impair its regenerative capacity and lead to liver failure. Mesenchymal stem cells (MSCs) have shown promise in liver regeneration, and induced pluripotent stem cells have made progress in organoid establishment and drug screening.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Ling Chen, Zhongwen Li, Wumei Wei, Bin An, Yao Tian, Wenjing Liu, Shuaishuai Niu, Yukai Wang, Liu Wang, Wei Li, Jie Hao, Jun Wu
Summary: Acute liver failure (ALF) is a clinical syndrome with high mortality. Liver transplantation is the only curative treatment for ALF, thus there is an urgent need for innovative therapies. This study evaluated the use of immunity-and-matrix regulatory cells (IMRCs) derived from human embryonic stem cells in treating ALF and investigated the underlying mechanism. The results showed that IMRCs improved liver histopathological changes, reduced liver enzyme levels in serum, promoted cell renewal, and protected against CCl4-induced liver damage.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Review
Immunology
Sho Tanosaki, Shugo Tohyama, Yoshikazu Kishino, Jun Fujita, Keiichi Fukuda
Summary: Pluripotent stem cells (PSCs) have promising applications in regenerative therapy, drug discovery, and disease modeling, but their metabolism differs greatly from that of somatic cells, requiring distinct features to sustain their unique properties.
INFLAMMATION AND REGENERATION
(2021)
Review
Cell Biology
Yiyi Xuan, Bjorn Petersen, Pentao Liu
Summary: This review comprehensively discusses the potential applications of human and pig pluripotent stem cells (PSCs) and provides an overview of the current progress and challenges. It explores the therapeutic uses of PSC-derived cellular products and emphasizes the importance of pig PSCs in genetic engineering for pig-to-human xenotransplantation. Further investigations and refinement of PSC technologies are necessary to unlock their full potential in regenerative medicine.
Article
Chemistry, Multidisciplinary
Saeed Abbasalizadeh, Sahab Babaee, Reza Kowsari-Esfahan, Zahra Mazidi, Yichao Shi, Jake Wainer, Joaquim M. S. Cabral, Robert Langer, Giovanni Traverso, Hossein Baharvand
Summary: Organoid medicine has made significant progress in the past decade as a novel therapeutic approach for addressing unmet medical needs, such as chronic liver disease. Through the use of scalable and xeno-free differentiation platforms, hepatic progenitors, mesenchymal stromal cells, and endothelial cells are generated from human pluripotent stem cell lines to produce vascularized liver organoids. A scalable microfluidic system is developed to generate cells-loaded microcapsules, which support cell proliferation and the self-organization of liver organoids using a self-biodegradable peptide hydrogel. These vascularized hepatobiliary organoids exhibit key functional features similar to fetal and adult liver tissue, making them promising for liver organoid medicine and drug development.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Computer Science, Interdisciplinary Applications
Yusuke Hayashi, Ikki Horiguchi, Masahiro Kino-oka, Hirokazu Sugiyama
Summary: This study presents a model-based assessment of temperature profiles in slow freezing for human induced pluripotent stem cells, which can estimate cell survival rate and required freezing time based on given freezing temperature profiles to achieve quality and productivity objectives. Simulation results indicate different cooling rates in dehydration, nucleation-promoting, and further cooling zones.
COMPUTERS & CHEMICAL ENGINEERING
(2021)
Review
Cell Biology
Michal S. Lach, Monika A. Rosochowicz, Magdalena Richter, Inga Jagiello, Wiktoria M. Suchorska, Tomasz Trzeciak
Summary: The development of induced pluripotent stem cells has opened up unlimited possibilities for regenerative medicine. This technology can be used to treat skeletal diseases such as osteoarthritis and has the potential to replace current short-term solutions. Additionally, using patient-specific tissue for reprogramming purposes can provide a platform for drug screening and modeling skeletal abnormalities.
