Article
Microbiology
Pottathil Shinu, Anroop B. Nair, Snawar Hussain, Mohamed A. Morsy, Wafaa E. Soliman
Summary: This study evaluated the performance of two different digestion and decontamination methods for the isolation of Mycobacteria from clinically suspected pulmonary tuberculosis patients, and found that pancreatin-CPC DDM showed competitive diagnostic accuracies compared to NALC-NaOH DDM in isolating Mycobacterial species.
Article
Microbiology
Amer El Ghali, Taylor Morrisette, Sara Alosaimy, Kristen Lucas, Maria G. Tupayachi-Ortiz, Raaga Vemula, Carly Wadle, Julie V. Philley, Carlos Mejia-Chew, Yasir Hamad, Ryan W. Stevens, John D. Zeuli, Andrew J. Webb, Christina T. Fiske, Anahit Simonyan, Christo L. Cimino, Mehriban Mammadova, Virginia E. Umana, Rodrigo Hasbun, Saira Butt, Kyle C. Molina, Michael Thomas, Emily A. Kaip, Jeannette Bouchard, Tristan W. Gore, Catessa Howard, M. Gabriela Cabanilla, Dana J. Holger, Jeremy J. Frens, Melissa Barger, Aaron Ong, Keira A. Cohen, Michael J. Rybak
Summary: This multicenter retrospective study examined the long-term clinical success, safety, and tolerability of Omadacycline (OMC) for nontuberculous mycobacteria (NTM) infections. The study found that 80.0% of patients achieved clinical success after 3 months of treatment, but 32.0% experienced adverse events related to OMC.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Antonio Broncano-Lavado, Laura Barrado, Paula Lopez-Roa, Juana Cacho, Diego Domingo, Sara Hernandez, Jesus Garcia-Martinez, Maria Rosario Millan, Elisa Perez-Cecilia, Maria-Jesus Ruiz-Serrano, Santiago Salso, Maria Simon, Marta Tato, Carlos Toro, Jose F. F. Valverde-Canovas, Jaime Esteban
Summary: NTM Elite agar is specifically designed for the isolation of NTM without the decontamination step, and has shown promising clinical performance in recovering NTM species.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Microbiology
Xin Ding, Yougui Yang, Yingshu Zhang, Qiang Zhang, Fanzhen Mao, Yang Dai
Summary: This study established and evaluated a novel nucleic acid detection method based on recombinase-aided isothermal amplification (RAA) for the detection and species identification of hookworm infections. Results showed that the RAA method had high sensitivity and specificity, and was faster and simpler than traditional microscopic techniques.
Article
Chemistry, Multidisciplinary
Min Jia Khor, Agnieszka Broda, Markus Kostrzewa, Francis Drobniewski, Gerald Larrouy-Maumus
Summary: Rapid diagnosis of bacterial infections is crucial for successful treatment. Traditional methods relying on protein detection may not be able to distinguish subspecies. This study proposes a new method using ethanol with a specific matrix for accurate subspecies identification.
FRONTIERS IN CHEMISTRY
(2021)
Article
Environmental Sciences
Qiumei He, Zizeng Lin, Peng Dong, Wenwen Tang
Summary: This study investigated the decontamination performance of a bioretention system using a sand-based filler. The results showed that the sand-based filler had a satisfactory overall removal effect, especially in removing suspended solids and phosphorus. The addition of inorganic constituents further improved the decontamination effect.
ENVIRONMENTAL TECHNOLOGY
(2022)
Article
Cell Biology
Fabio A. Abade dos Santos, C. L. Carvalho, Isabel Almeida, Teresa Fagulha, Fernanda Rammos, Silvia C. Barros, Margarida Henriques, Tiago Luis, Margarida D. Duarte
Summary: To overcome the limited availability of commercial hare and rabbit immortalized cell lines, researchers can establish primary cell cultures derived from biopsies or embryos using a fast and inexpensive method involving dispase II and trypsin. This approach allows for an in vitro cell culture system with high viability yield and purity level, suitable for clinical and fundamental research as well as diagnosis.
Article
Virology
Marge Kartau, Eeva Auvinen, Auli Verkkoniemi-Ahola, Laura Mannonen, Ilkka Helantera, Veli-Jukka Anttila
Summary: JCPyV DNA detection is most valuable in the diagnosis of neurological symptoms in immunosuppressed patients, but the value of plasma samples is disputed in the screening of JCPyV-associated nephropathy.
JOURNAL OF CLINICAL VIROLOGY
(2022)
Article
Infectious Diseases
Wei Wei, Jie Cao, Xiao-cui Wu, Li-ping Cheng, Xiao-na Shen, Wei Sha, Qin Sun
Summary: This study retrospectively analyzed data from 422 suspected NTMPD patients in Shanghai Pulmonary Hospital and compared the diagnostic performance of mNGS and MGIT 960. The results showed that mNGS had a higher sensitivity than MGIT 960 and a similar specificity. mNGS demonstrated effective performance in NTMPD diagnosis.
Article
Chemistry, Analytical
Alejandra Ben Aissa, Barbara Araujo, Esther Julian, Maria Valnice Boldrin Zanoni, Maria Isabel Pividori
Summary: This study successfully combines IMS and paper-based nucleic acid immunochromatographic assay for sensitive detection of Mycolicibacterium fortuitum. The preconcentration of bacteria and amplification of genome using specific primers were highlighted, along with the comparison of analytical performance of different immunochromatographic platforms.
Article
Chemistry, Analytical
Didem Rodoplu Solovchuk, Ismail Hakki Boyaci, Ugur Tamer, Nurettin Sahiner, Demet Cetin
Summary: In this study, a simple gradient centrifugation-based optical detection (GCB-OD) method was developed for bacteria testing in skim milk. The method involves capturing bacteria using nanoparticles and separating bacteria-captured particles through centrifugation. Images of sucrose gradients are captured using a simple mobile phone-setup, and the optical density of the particle-bound bacteria zones can be measured using MATLAB software. The choice of nanoparticles and sucrose gradient conditions were optimized for efficient detection of bacteria.
MICROCHEMICAL JOURNAL
(2023)
Article
Pharmacology & Pharmacy
Fadhli Adesta, Caroline Mahendra, Kathleen Irena Junusmin, Arya Melissa Selva Rajah, Sharon Goh, Levana Sani, Alexandre Chan, Astrid Irwanto
Summary: The survey results indicate a general agreement among healthcare professionals on the benefits of PGx testing. However, there is a lack of self-efficacy and competency in utilizing PGx data. A 90-minute training significantly improves these issues, leading to a greater anticipation of PGx adoption.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Infectious Diseases
Hei Man Wong, Cheok Hong Mun, Weng Keong Loke, Wei Qi Lim, Geraldine Wei Yen Chee, Sook Lan Tan, Jye Yng Teo, Yi Yan Yang, Hendrix Tanoto, Xian Jun Loh, Chen Ee Lee, Chuanwen Tiang, Wei Yee Wan, Charlene Cheong, Kue Bien How, Moi Lin Ling, Ban Hock Tan
Summary: This study systematically assessed the impact of repeated cycles of wear and decontamination on the integrity of N95 filtering facepiece respirators (FFRs). The results suggest that moist heat is a promising method for decontaminating N95 FFRs.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Masabumi Shibuya, Haruka Matsui, Tadashi Sasagawa, Takeshi Nagamatsu
Summary: sFLT1, a VEGF-trapping protein, is crucial for the physiological barrier between fetal and maternal angiogenesis in normal pregnancy, but its abnormal overexpression in preeclampsia can lead to severe complications. Early detection of increased serum sFLT1 is essential for proper medical care.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Savina Ditommaso, Monica Giacomuzzi, Gabriele Memoli, Jacopo Garlasco, Antonio Curtoni, Marco Iannaccone, Carla Maria Zotti
Summary: The aim of this study was to propose alternative culture techniques to improve non-tuberculous mycobacteria detection in environmental samples. The results indicate that NTM Elite agar provides a valid method, which does not involve a decontamination step and provides greater recovery efficiency by directly plating the filters on the media.
JOURNAL OF MICROBIOLOGICAL METHODS
(2022)