Article
Biochemistry & Molecular Biology
Eun Pyo Kim, Giup Jang, Jin-Wook Kim, Sang Wha Kim, Heaji Chung, Yun Jung Yang, Wan Hee Kim, Geon A. Kim
Summary: Canine mammary gland tumor is commonly diagnosed in intact female dogs. Early and accurate diagnosis is crucial for prognosis. This study analyzed differentially expressed microRNAs and mRNA levels to investigate the malignancy and biopsy locations of the tumors. The miRNA cfa-miR-503 showed altered expression in response to malignancy and could potentially be used as a diagnostic biomarker and anti-tumor drug.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Veterinary Sciences
Lei Yin, Xuehuai Shen, Danjun Zhang, Ruihong Zhao, Hongyan Hou, Xiaomiao Hu, Jieru Wang, Yin Dai, Xiaocheng Pan, Kezong Qi
Summary: In this study, miRNA-seq was used to analyze the expression changes of bone marrow miRNAs in chickens infected with Avian pathogenic Escherichia coli (APEC), revealing the role of these miRNAs in regulating the host immune response. Functional annotation analysis showed that the immune system process was significantly enriched at 12 and 24 hours post-infection, and several important signaling pathways were triggered. Real-time PCR validated the differential expression of miRNAs, and a miRNA-target gene network identified the potential roles of gga-miR-2127, gga-miR-6643-5p, and gga-miR-6567-3p in the immune process.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Immunology
Luyao Liu, Xi Chen, Ting Tang, Li Chen, Qiaoling Huang, Zhongyu Li, Qinqin Bai, Lili Chen
Summary: Using small RNA sequencing technology, a large amount of differentially expressed miRNAs were identified in human bronchial epithelial cells infected with Chlamydia psittaci. Functional annotation and pathway analysis revealed that these miRNAs mainly target genes involved in transcriptional regulation and ATP binding, with potential implications in key signaling pathways. The qRT-PCR results confirmed the consistency between the validated miRNAs and the sRNA sequencing data.
MICROBIAL PATHOGENESIS
(2021)
Article
Genetics & Heredity
Nana Ji, Liping Xiang, Bing Zhou, Yan Lu, Min Zhang
Summary: The study investigated the regulation of nutrient status in the liver during the Fed-Fast-Refed cycle. Transcriptomic analysis revealed dynamic alterations in mRNA and miRNA expression, particularly in lipid and protein metabolism pathways. The study also identified key modules and pathways involved in metabolism through protein-protein interaction and miRNA-mRNA networks.
FRONTIERS IN GENETICS
(2023)
Article
Biology
Masafumi Katayama, Tomokazu Fukuda, Takehito Kaneko, Yuki Nakagawa, Atsushi Tajima, Mitsuru Naito, Hitomi Ohmaki, Daiji Endo, Makoto Asano, Takashi Nagamine, Yumiko Nakaya, Keisuke Saito, Yukiko Watanabe, Tetsuya Tani, Miho Inoue-Murayama, Nobuyoshi Nakajima, Manabu Onuma
Summary: iPSCs from three endangered avian species were developed and characterized as a potential resource for their conservation. These iPSCs showed pluripotency markers, expressed pluripotency-related genes, and differentiated into three germ layers. Despite using the same reprogramming factors, the iPSCs from Japanese ptarmigan displayed different cellular characteristics and contributed to chimeras in chicken embryos. This study provides the first evidence of the potential value of iPSCs as a resource for endangered avian species conservation.
COMMUNICATIONS BIOLOGY
(2022)
Article
Obstetrics & Gynecology
Jiao Yu, Dongshu Zhu, Cheng Zeng, Yangyang Zhang, Huixia Yang, Yang Xu
Summary: The study found that the Progesterone primed ovarian stimulation (PPOS) protocol can influence the expression profiles of microRNAs in the granulosa cells of the follicular fluid in infertile patients. The up-regulation of miR-6869-5p and down-regulation of miR-4261 were consistently observed. The enrichment analysis suggests that these regulatory effects may be involved in cell proliferation, apoptosis, and signaling pathways.
EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Elizabeth Hutchins, Rebecca Reiman, Joseph Winarta, Taylor Beecroft, Ryan Richholt, Matt De Both, Khalouk Shahbander, Elizabeth Carlson, Alex Janss, Ashley Siniard, Chris Balak, Ryan Bruhns, Timothy G. Whitsett, Roger McCoy, Matthew Anastasi, April Allen, Brian Churas, Matthew Huentelman, Kendall Van Keuren-Jensen
Summary: This study collected plasma and urine samples from healthy volunteers to determine circRNA content using RNASeq. Highly expressed circRNA junctions were validated and a higher ratio of circular to linear transcripts was found in plasma compared to urine. Longitudinal samples showed stable circRNA expression over time.
Article
Multidisciplinary Sciences
Yerin Kim, Namhee Yu, Ye Eun Jang, Eunkyung Lee, Yeonjoo Jung, Doo Jae Lee, W. Robert Taylor, Hanjoong Jo, Jaesang Kim, Sanghyuk Lee, Sang Won Kang
Summary: Endothelial dysfunction and inflammatory immune response induce dedifferentiation of vascular smooth muscle cells (SMCs) and contribute to arterial occlusion. This study reveals that specific miRNAs are upregulated in injured rat carotids and synthetic-type human vascular SMCs. Inhibiting these miRNAs prevents neointimal hyperplasia in injured rat carotid arteries and directs SMC proliferation. Furthermore, bone morphogenic protein (BMP)-7 is identified as a significant target gene regulated by miR-370-3p, influencing SMC proliferation in both rodent and human systems.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Daisuke Saito, Ryosuke Tadokoro, Arata Nagasaka, Daisuke Yoshino, Takayuki Teramoto, Kanta Mizumoto, Kenichi Funamoto, Hinako Kidokoro, Takaki Miyata, Koji Tamura, Yoshiko Takahashi
Summary: Unlike mammals, avian early embryos have PGCs that utilize blood circulation to migrate to the somatic gonadal primordium. Our research shows that PGCs are arrested at a specific site in the capillary plexus, enabled by increased stiffness mediated by actin polymerization. They then soften to squeeze through the endothelial lining.
Article
Multidisciplinary Sciences
Haijun Zhu, Mengshi Kang, Xinping Bai
Summary: The study confirmed that miR-10a-5p had lower expression in melanoma cells and high expression suppressed the proliferation, migration, and invasion abilities. It was also found that miR-10a-5p targeted LIN28B mRNA and decreased LIN28B protein expression. TCF21 upregulation led to decreased LIN28B expression and increased miR-10a-5p expression, revealing a potential regulatory relationship between TCF21 and miR-10a-5p in melanoma.
Article
Oncology
Mateusz Sikora, Katarzyna Krajewska, Klaudia Marcinkowska, Anna Raciborska, Rafal Jakub Wiglusz, Agnieszka Smieszek
Summary: Osteosarcoma is a malignant bone tumor that mainly affects children and elderly people. The primary treatment methods for osteosarcoma are still underdeveloped, leading to poor survival rates. This study aimed to establish the expression profile of molecular markers related to osteosarcoma survival, progression, and metastasis. The findings may facilitate the selection of reliable cellular models for pre-clinical investigations on osteosarcoma therapy development.
Article
Veterinary Sciences
Baohui Yao, Yukun Kang, Kang An, Yuchen Tan, Qiqi Hou, Degang Zhang, Junhu Su
Summary: In this study, the differentially expressed miRNAs, miRNA-mRNA interaction network, and function of testicular cells in plateau zokor during the breeding season were comprehensively analyzed. A total of 381 known and 94 novel miRNAs were identified. Compared to breeders, non-breeders showed a different expression pattern of miRNAs. The target mRNAs of these differentially expressed miRNAs were related to testicular development, spermatogenesis, and energy metabolism. PROK2 was identified as a target mRNA of rno-miR-143-3p. This study provides insights into the regulatory mechanisms of miRNAs in testicular development and spermatogenesis under reproductive suppression.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Oncology
Xinke Xu, Hongyao Yuan, Junping Pan, Wei Chen, Cheng Chen, Yang Li, Fangcheng Li
Summary: This study investigated the miRNAome and transcriptome traits of AT/RT, identifying differentially expressed miRNAs and mRNAs. The dysregulation of miR-17-5p may play a pivotal role in AT/RT, suggesting potential therapeutic targets and diagnostic biomarkers. These findings provide insights into the pathogenesis of AT/RT and highlight the importance of miRNAs as potential therapeutic targets and diagnostic tools.
Article
Biotechnology & Applied Microbiology
Jinjiao Zuo, Yanan Cao, Zhisheng Wang, Abid Ullah Shah, Wenlei Wang, Chen Dai, Mingjia Chen, Jian Lin, Qian Yang
Summary: IBV inhibits the antigen-presentation ability of avian dendritic cells by suppressing their maturation, cytokine secretion, and antigen presentation.
Article
Multidisciplinary Sciences
Hang Jie, Zhongxian Xu, Jian Gao, Feng Li, Yinglian Chen, Dejun Zeng, Guijun Zhao, Diyan Li
Summary: In this study, Illumina deep sequencing technology was used to analyze the small RNA transcriptome of unmated and mated male Chinese forest musk deer, revealing 1,652 known miRNAs and 45 novel miRNAs with differential expression. Target gene functional enrichment in unmated males showed more involvement in structural molecule activity and cellular exocytosis processes, suggesting a potential role of these genes in musk formation. Further investigation into specific miRNA-mRNA pairs may shed light on the development and secretion of musk gland in this species.