Review
Biochemistry & Molecular Biology
Huimin Pang, Jinwen Li, Shu Jie Li
Summary: This review summarizes advances made so far in our understanding of the roles of Hv1 in the regulation of insulin secretion in beta cells, glucose homeostasis, and obesity.
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Review
Neurosciences
Yu Shen, Yuncheng Luo, Ping Liao, Yunxia Zuo, Ruotian Jiang
Summary: Hv1 is the only voltage-gated proton-selective channel in mammalian cells. It primarily functions to extrude protons from the cytoplasm upon pH reduction and membrane depolarization. Accumulating evidence shows its expression in the nervous system and its involvement in various neurological diseases.
NEUROSCIENCE BULLETIN
(2023)
Article
Immunology
Hongyan Du, Huimin Pang, Yingtang Gao, Yongfa Zhou, Shu Jie Li
Summary: This study demonstrates that Hv1 deficiency exacerbates OVA-induced allergic lung asthma in mice by increasing ROS production.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Yoshifumi Okochi, Yasushi Okamura
Summary: The voltage-gated proton channel Hv1, also known as VSOP, regulates reactive oxygen species (ROS) production and phagosomal pH maintenance, while promoting calcium influx at the plasma membrane in phagocytes. It serves as both a positive and negative regulator of ROS generation in neutrophils, inhibiting hypochlorous acid production and ERK signaling to reduce inflammation upon fungal infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Juan J. Alvear-Arias, Antonio Pena-Pichicoi, Christian Carrillo, Miguel Fernandez, Tania Gonzalez, Jose A. Garate, Carlos Gonzalez
Summary: The acid-base characteristics of tumor cells and the tumor microenvironment have attracted scientific interest. The voltage-gated proton channel (Hv1) plays a crucial role in maintaining pH balance and has been found to be overexpressed in acidic tumor environments. Evidence suggests that the Hv1 channel is involved in the development of malignancy features in solid tumors, making it a potential therapeutic target.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Cell Biology
Petronel Tuluc, Tamara Theiner, Noelia Jacobo-Piqueras, Stefanie M. Geisler
Summary: Pancreatic islets secrete hormones critical for glucose homeostasis, with insulin from beta-cells being the main hormone for lowering blood glucose levels. Glucagon and somatostatin also play important roles in regulating hormone secretion. High voltage-gated Ca2+ channels are central in the release of pancreatic hormones and play a key role in diabetes.
Article
Immunology
Yun Li, Rodney M. Ritzel, Junyun He, Tuoxin Cao, Boris Sabirzhanov, Hui Li, Simon Liu, Long-Jun Wu, Junfang Wu
Summary: Tissue acidosis is a crucial secondary injury process in traumatic spinal cord injury (SCI). This study demonstrates that Hv1 proton channel mediates hydrogen proton extrusion after SCI, contributing to increased tissue acidosis and poor long-term outcomes. Hv1 deficiency leads to reduced acidosis, ROS production, and improved functional outcomes, suggesting its potential as a therapeutic target for SCI.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Biochemistry & Molecular Biology
Marco Cozzolino, Adrienn Gyongyosi, Eva Korpos, Peter Gogolak, Muhammad Umair Naseem, Judit Kallai, Arpad Lanyi, Gyorgy Panyi
Summary: Myeloid-derived suppressor cells (MDSCs) play a key role in the immunosuppressive microenvironment of tumors. This study investigated the ion channel repertoire in tumor-derived PMN-MDSC and Mo-MDSCs, and found that Hv1 H+ channel is expressed in both types of MDSCs. Hv1 might be a potential target for inhibiting MDSCs in tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Neurosciences
Junyun He, Rodney M. Ritzel, Junfang Wu
Summary: Hv1 is a newly discovered, highly conserved ion channel present in various species, with a key role in regulating ROS and cytosolic pH. Studies suggest that proton currents through Hv1 are crucial for excessive ROS production in CNS injury, and targeting Hv1 may provide neuroprotection in such conditions.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Endocrinology & Metabolism
Nizar I. Mourad, Daela Xhema, Pierre Gianello
Summary: The study investigated the glucagon secretion characteristics of pig islet cells and found that high glucose concentration significantly inhibited glucagon secretion. In vivo experiments showed a quick decrease in plasma glucagon and a slower increase in plasma insulin after glucose injection.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell Biology
Erwin Ilegems, Galyna Bryzgalova, Jorge Correia, Burcak Yesildag, Edurne Berra, Jorge L. Ruas, Teresa S. Pereira, Per-Olof Berggren
Summary: This study found the presence of HIF-1 alpha in pancreatic cells during the progression of type 2 diabetes, and demonstrated that treatment with the HIF-1 alpha inhibitor PX-478 can improve beta cell function, prevent diabetes progression, and increase insulin secretion. The results showed that PX-478 treatment has an improving effect on mouse and human islets.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Md Mostafizur Rahman, Asmita Pathak, Kathryn L. Schueler, Haifa Alsharif, Ava Michl, Justin Alexander, Jeong-A Kim, Sushant Bhatnagar
Summary: Stimulus-coupled insulin secretion involves fusion of insulin granules to the plasma membrane via SNARE complex formation. Deletion of Syt9 protein increases glucose clearance and plasma insulin levels in mice. Syt9 interacts with tomosyn-1 and Stx1A, and its inhibitory effects on insulin secretion are mediated by tomosyn-1.
Article
Biochemistry & Molecular Biology
Md Mostafizur Rahman, Asmita Pathak, Kathryn L. Schueler, Haifa Alsharif, Ava Michl, Justin Alexander, Jeong-A Kim, Sushant Bhatnagar
Summary: This study shows that the deletion of Synaptotagmin-9 (Syt9) in mice increases glucose clearance and plasma insulin levels, inhibiting insulin secretion and glucose clearance.
Article
Endocrinology & Metabolism
Lucie Oberhauser, Cecilia Jimenez-Sanchez, Jesper Grud Skat Madsen, Dominique Duhamel, Susanne Mandrup, Thierry Brun, Pierre Maechler
Summary: Chronic exposure to high glucose and fatty acids can lead to adaptations in pancreatic beta cells, which help preserve their secretion response. The expression pattern of lipid pathway genes and the process of fat storage and mobilization play a crucial role in glucose-stimulated insulin secretion.
Review
Cell Biology
Jiaying Zheng, Madhuvika Murugan, Lingxiao Wang, Long-Jun Wu
Summary: Microglia play a crucial role in spinal cord injury, but excessive activation can cause secondary damage. The voltage-gated proton channel Hv1 is found to regulate microglial activation and its deficiency can alleviate secondary damage and improve neuronal recovery.
NEURAL REGENERATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)