Review
Oncology
Meysam Najaflou, Mehdi Shahgolzari, Ahmad Yari Khosroushahi, Steven Fiering
Summary: Tumor cell-derived extracellular vesicles (TEVs) play a significant role in tumor communication with the patient's physiology and can influence tumor regression or immune escape. Understanding the immunotherapeutic properties of TEVs and their manipulation for cancer treatment is a new research approach.
Review
Pharmacology & Pharmacy
Yarong Zhao, Luotong Liu, Rongze Sun, Guilin Cui, Shuyu Guo, Songren Han, Ziwei Li, Tian Bai, Lesheng Teng
Summary: As an important means of communication among cells, exosomes are increasingly studied, especially in the context of cancer immunotherapy. Exosomes derived from tumor cells and different immune cells have complex and changeable physiological functions due to carrying different proteins and nucleic acids from the source cells. Based on the role of exosomes in intercellular communication, cancer treatment methods are continuously being researched. This review briefly introduces the molecular composition of exosomes and its secretion mechanism, summarizes the role of exosomes encapsulating different information molecules, and provides a detailed introduction to the role of exosomes in the three phases of tumor immunoediting and the relevant literature of exosomes in the tumor immune microenvironment.
ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Constantin Schmitt, Dieter Saur, Stefanie Baerthel, Chiara Falcomata
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a complex disease with a heterogeneous tumor microenvironment. Mouse models, such as immunocompetent and cell line-based orthotopic allograft models, are widely used for preclinical studies. This protocol provides a reproducible and robust method for injecting PDAC cells into the mouse pancreas.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Oncology
Xingxing Chen, Xuehua Lin, Guofu Pang, Jian Deng, Qun Xie, Zhengrong Zhang
Summary: The study revealed that frequent KDM6A mutations in bladder cancer promote tumor immune escape, serving as a potential novel biomarker for predicting the immune response.
Article
Cell Biology
Tianyao Liu, Xinyan Xu, Jiazheng Li, Ming Bai, Wenjie Zhu, Yanqing Liu, Siyang Liu, Zihan Zhao, Tianhang Li, Ning Jiang, Yuhao Bai, Qingyang Jin, Yulin Zhang, Yufeng Zheng, Shengkai Zhou, Shoubin Zhan, Ying Sun, Gaoli Liang, Yang Luo, Xi Chen, Hongqian Guo, Rong Yang
Summary: This study uncovers a novel mechanism for BCa ferroptosis escape and demonstrates the crucial role of ALOX5 in BCa resistance to ferroptosis, suggesting that ALOX5 may be a potential therapeutic target and prognostic biomarker in BCa treatment.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Yi Jiang, Zhenhao Zeng, Situ Xiong, Ming Jiang, Gaomin Huang, Chiyu Zhang, Xiaoqing Xi
Summary: The study identified two distinct immune clusters in BLCA patients, characterized by high and low ImmuneScores. CD96 and IBSP were identified as prognostic genes. ImmuneScoreH showed inactivation of innate immune cell chemotaxis, while ImmuneScoreL exhibited low tumor antigen load as a potential immune escape mechanism.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Zhiduo Sun, Weiyun Zhang, Zhichao Ye, Li Yuan, Manli Fu, Xiaoming Liu, Huageng Liang, Heyou Han
Summary: This study presents the design of a novel nanocarrier DNAH for the treatment of bladder cancer, utilizing photothermal therapy to overcome the bladder mucosal barrier and enhance drug penetration. The results demonstrate the potential inhibition of bladder tumor growth by DNAH, leading to improved survival time in mice.
Article
Chemistry, Multidisciplinary
Bowen Sun, Juwita Norasmara Bte Rahmat, Han Joon Kim, Ratha Mahendran, Kesavan Esuvaranathan, Edmund Chiong, John S. Ho, Koon Gee Neoh, Yong Zhang
Summary: A novel approach combining photodynamic therapy and chemotherapy is proposed in this study, which utilizes nanocarriers and an LED light source to deliver drugs precisely to tumor sites, significantly improving therapeutic outcomes.
Article
Oncology
Junlong Zhuang, Lan Shen, Meiqian Li, Jingya Sun, Jiange Hao, Jiaxuan Li, Zhen Zhu, Shuning Ge, Dianzheng Zhang, Hongqian Guo, Ruimin Huang, Jun Yan
Summary: Cancer-associated fibroblasts (CAF) promote cancer stemness and chemoresistance in urothelial bladder cancer (UBC) by overexpressing miR-146a-5p. MiR-146a-5p also enhances UBC stemness and chemoresistance by targeting ARID1A and PRKAA2 mRNA in UBC cells. Elevated levels of exosomal miR-146a-5p in UBC patients' serum are associated with tumor stage and relapse risk.
Article
Veterinary Sciences
Francesca La Cava, Alberto Fringuello Mingo, Sonia Colombo Serra, Aldo Di Vito, Claudia Cabella, Paolo Oliva, Alessia Cordaro, Chiara Brioschi, Enzo Terreno, Luigi Miragoli
Summary: Breast cancer is the most common cancer among women worldwide, and CE-MRI is recommended as supplemental screening for high-risk women. This study developed a refined method to orthotopically induce breast cancer in mice and used CE-MRI to characterize the evolution of the tumoral lesion.
LABORATORY ANIMALS
(2021)
Article
Genetics & Heredity
Michael Wessolly, Fabian D. Mairinger, Thomas Herold, Boris Hadaschik, Tibor Szarvas, Henning Reis
Summary: In recent years, treatment options for advanced bladder cancer have been rapidly evolving. Predictive biomarkers are urgently needed to select effective therapy and minimize side effects. Immune system-based biomarkers show promise, particularly in relation to altered proteasomal epitope processing and immune escape mechanisms.
Article
Oncology
Ulrich Jarry, Megane Bostoen, Raphael Pineau, Laura Chaillot, Valentine Mennessier, Pierre Montagne, Emilie Motte, Marjorie Gournay, Arnaud Le Goff, Thierry Guillaudeux, Remy Pedeux
Summary: An orthotopic NSCLC model was established by injecting luciferase-expressing PC-9 cells into the tail vein of mice, allowing evaluation of tumor growth and response to osimertinib treatment. Micro-metastases were detected by bioluminescence imaging and collected for further analysis, providing new tools to study tumor progression and drug resistance in NSCLC.
Article
Engineering, Environmental
Fengqiu Hong, Xiaorui Geng, Guangzong Min, Xiang Sun, Bei Zhang, Youliang Yao, Ronghui Li, Jing Wang, Hengyu Zhao, Peng Guo, Zhen Yuan, Xiaofei Wen, Liming Nie, Gang Liu, Xiaoyuan Chen, Qingliang Zhao
Summary: This study proposed a strategy that combines dual-modality three-dimensional (3D) NIR-II photoacoustic (PA) and optical coherence tomography angiography (OCTA) molecular imaging to guide visually interventional photothermal therapy (PTT) for bladder cancer (BC). Furthermore, an excellent NIR-II hyaluronic acid-IR-1048 with liposome-coated (HAPO-1048) photothermal agent was developed for PTT of CD44 overexpressing orthotopic BC. The results showed high sensitivity and specificity in dynamic monitoring and investigating the precision therapy of PTT in deep orthotopic BC. Additionally, OCTA was used to monitor and assess the microvascular morphometrics and angiogenesis of BC.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Chenfei Zhou, Wenfei Wei, Jing Ma, Yang Yang, Luojiao Liang, Yanmei Zhang, Zici Wang, Xiaojing Chen, Lei Huang, Wei Wang, Sha Wu
Summary: The study suggests that miR-1468-5p in cervical cancer exosomes promotes lymphatic PD-L1 upregulation and lymphangiogenesis, leading to T cell immunity suppression and allowing cancer cells to evade anti-cancer immunity. High levels of exosomal miR-1468-5p in serum correlate with immunosuppressive status in the tumor microenvironment and poor prognosis in cervical cancer patients.
Article
Biochemistry & Molecular Biology
Jiancheng Lv, Qikai Wu, Kai Li, Kexin Bai, Hao Yu, Juntao Zhuang, Huanyou Sun, Haiwei Yang, Xiao Yang, Qiang Lu
Summary: This study identified a significant correlation between lysine N-methyltransferase SETD7 and immunotherapy sensitivity and prognosis in patients with bladder cancer. SETD7 was found to be highly expressed in bladder cancer and was associated with high histological grade and worse prognosis. Mechanistically, SETD7 promoted the proliferation, migration, and immune escape of bladder cancer cells through the upregulation of STAT3 and PD-L1.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)