Article
Engineering, Chemical
Sally A. Ali
Summary: The treatment of azo dye effluent and the sustainable development of the environment can be achieved through the use of green mycoremediation. Trichothecium roseum demonstrated excellent decolourization capability for Azo Lanasyn Navy M-DNL dye, with a decolourization percentage of 94%. It was also shown that the dye was adsorbed onto the surface of T. roseum. The immobilised T. roseum cells exhibited a high biosorption percentage for the dye under optimized conditions.
Article
Agronomy
Qili Liu, Qianqian Zhang, Huali Xue, Yang Bi, Xi Yang, Yuanyuan Zong, Zhiguang Liu, Jiangyang Chen, Dov Prusky
Summary: Phospholipase D (PLD) plays a crucial role in the pathogen-host interaction by hydrolyzing the phosphodiester bond in the host cell membrane and producing phosphatidic acid, which interacts with NADPH oxidase (NOX) to generate reactive oxygen species (ROS) and enhance pathogenicity. However, the role of PLD in filamentous fungi and its impact on postharvest fruit and vegetables is unknown. In this study, mutants of ATrPLD1 and ATrPLD2 were created in Trichothecium roseum and their effects on ROS production and pathogenicity on apple fruit were analyzed. The results showed that the deletion of TrPLD1 and TrPLD2 increased sensitivity to oxidative stress and reduced ROS production in T. roseum. The ATrPLD1 mutant exhibited decreased NOX expression and activity, resulting in a lower production of superoxide anion during infection, while the expression and activity of superoxide dismutase and peroxidase were up-regulated and catalase was down-regulated. Ultimately, this enhanced the production of H2O2 in apple tissue, which acted as a signal molecule triggering disease resistance and reducing the pathogenicity on apple fruit. On the other hand, the ATrPLD2 mutant had no significant effect on ROS production during pathogen infection. In conclusion, ATrPLD1 and ATrPLD2 mutants showed high sensitivity to oxidative stress, with TrPLD1 playing a more important role in regulating ROS production in T. roseum infected apple fruit.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Microbiology
Bin Wang, Zhanhong Han, Di Gong, Xiaobin Xu, Yongcai Li, Edward Sionov, Dov Prusky, Yang Bi, Yuanyuan Zong
Summary: pH is an important environmental factor that affects the growth, development, and pathogenicity of postharvest pathogens. The transcription factor PacC plays a key role in the pH signaling pathway. Trichothecium roseum, an alkalizing fungus, shows increased expression of TrPacC in response to acidic environments. TrPacC positively regulates fungal growth, development, and pathogenicity, as well as the fungal response to different stresses. The Delta TrPacC mutant exhibits reduced sporulation, delayed spore germination, increased sensitivity to ionic stress, and decreased pathogenicity to muskmelon and tomato fruits.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Agronomy
Bin Wang, Xingfen He, Zhanhong Han, Yuanyuan Zong, Huali Xue, Oyom William, Edward Sionov, Dov Prusky, Yang Bi
Summary: The Pal signaling pathway is crucial for the adaptation of pathogenic fungi to pH fluctuations and involves several components including PacC. This study investigated the role of TrPacC in Trichothecium roseum, revealing its involvement in pH signal perception, ammonia synthesis, and ROS production. Deletion of TrPacC downregulated the expression of genes related to PacC activation and ammonia synthesis, leading to reduced ammonia accumulation and alkalization ability. Furthermore, TrPacC deletion reduced ROS levels and inhibited the expression of extracellular enzyme genes, resulting in reduced pathogenicity of T. roseum on muskmelon and apple fruit.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Shujuan Wu, Jianmin Yun, Rui Wang, Wenwei Zhang, Lin Hao, Pengzheng Pei
Summary: This study investigated the antifungal activity and mode of action of Bacillus pumilus HN-10 antifungal peptide P-1 against Trichothecium roseum. The results showed that P-1 had strong antifungal activity against T. roseum and achieved its inhibitory effect mainly by regulating genes related to energy metabolism.
Article
Plant Sciences
Lei Zhu, Zhaoyuan Wu, Wei Fang, Yueying Wang
Summary: In this study, a high-quality genome assembly of strain YXFP-22015 of T. roseum was reported, using Oxford Nanopore Technologies (ONT) for long read sequencing and MGISEQ-2000 for short read sequencing. The genome was also well-annotated based on RNA-seq by MGISEQ-2000 and in silico prediction. Further analysis will contribute to a better understanding of T. roseum infection mechanisms.
Article
Food Science & Technology
Mo Zhu, Xiao Duan, Pengkun Cai, Wanwan Zhang, Yan Liu, Jiangkuan Cui, Zhengnan Li, Zongbo Qiu
Summary: Trichothecium roseum is a mycoparasite that inhibits phytopathogenic fungi. This study identified T. roseum as a mycoparasite on wheat powdery mildew fungus and demonstrated its ability to impair colony formation and conidial distribution of the fungus. A T. roseum strain expressing green fluorescent protein was produced using transformation technology to visualize the interaction between T. roseum and the fungus. These findings suggest that T. roseum could be a potential biological agent for managing wheat powdery mildew.
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2022)
Article
Agronomy
Qianqian Zhang, Qili Liu, Huali Xue, Yang Bi, Zhiguang Liu, Mina Nan
Summary: Trichothecium roseum is a postharvest pathogenic fungus that infects various fruits and vegetables, causing postharvest diseases and posing a health threat due to mycotoxin accumulation. Phospholipase D (PLD) plays a crucial role in pathogen-host interactions by damaging the cell membrane. In this study, the effect of PLD inhibitor 1-butanol on T. roseum growth and pathogenicity on muskmelon fruit was evaluated. The results showed that 0.5% 1-butanol significantly inhibited spore germination, germ tube length, hyphal growth, and sporulation of T. roseum, and reduced PLD activity in treated fruits. TrPLD gene expression analysis suggested that TrPLD3 played a more crucial role in the pathogenicity reduction.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zhanhong Han, Zhenyu Wang, Yang Bi, Yuanyuan Zong, Di Gong, Bin Wang, Baojun Li, Edward Sionov, Dov Prusky
Summary: The research findings show that the pH modulation by Trichothecium roseum affects the colonization ability of the fungus and the host response, including the production and scavenging of reactive oxygen species in apple fruits. Inoculation at pH 3 results in lower cell membrane permeability and oxidative stress, while enhancing the activity of antioxidant enzymes in the fruit tissue.
Article
Materials Science, Multidisciplinary
Fupeng Zhu, Yan Zhu, Yuanshou Zhao, Fu Chen, Wenjun Sheng, Wei Zhang, Pengqing Wang, Jiangwen Deng, Yunyu Sun, Weibing Zhang, Yongcai Li
Summary: This study found that 2',4'-dichloro-chalcone is effective in inhibiting the growth and pathogenicity of Fusarium tricinctum and Trichothecium roseum, and activates the cyanide-resistant respiratory pathway in both pathogens. Additionally, it significantly inhibits the spread of diseases caused by these pathogens. Therefore, this substance has the potential to be an alternative safe method for controlling postharvest diseases.
Article
Multidisciplinary Sciences
Dulanjalee Lakmali Harishchandra, Wei Zhang, Thilini Chethana Kandawatte Wedaralalage, Xinghong Li, Ratchadawan Cheewangkoon
Summary: Grape is an important crop that is consumed fresh and used in various secondary products. Postharvest losses in grapes can be caused by pathogenic infections and improper handling. This study reports the occurrence of postharvest pink mold rot caused by Trichothecium roseum on grapes, along with molecular phylogeny, morphological studies, and pathogenicity tests. This is also the first report of T. roseum from grapes in China.
CHIANG MAI JOURNAL OF SCIENCE
(2023)
Article
Food Science & Technology
Laura Rustioni, Alessio Altomare, Gvantsa Shanshiashvili, Fabio Greco, Riccardo Buccolieri, Ileana Blanco, Gabriele Cola, Daniela Fracassetti
Summary: This research evaluates the composition of wines made with white grapes susceptible to sunburn symptoms. The canopy management of 'Verdeca' grapevines in South Italy was studied, with grapes vinified under different conditions. The quality and quantity of grapes produced were significantly affected, with smaller sunburned berries found on unshaded bunches. The winemaking process also had an impact on the resulting wines, with higher pH and extraction of phenolics observed in wines made with sunburned grapes.
Article
Chemistry, Multidisciplinary
Simona Gavrilas, Ioan Calinovici, Sabin Chis, Claudiu-Stefan Ursachi, Mirabela Rat, Florentina-Daniela Munteanu
Summary: This study explores the feasibility of using waste for wastewater treatment. The potential of grape pomace for wastewater remediation is evaluated through testing its antioxidant properties and organic phosphorus compound concentration.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Applied
Elia Romanini, Jacqui M. McRae, Eleanor Bilogrevic, Donato Colangelo, Mario Gabrielli, Milena Lambri
Summary: The study found that grape seed powder can effectively remove pathogenesis-related proteins in wine and reduce haze formation. Treatment with high doses of GSP resulted in higher scores in color and bitterness, while low doses had a smaller impact on these attributes.
Article
Food Science & Technology
Vesna Milanovic, Federica Cardinali, Ilario Ferrocino, Ana Boban, Irene Franciosa, Jasenka Gajdos Kljusuric, Ana Mucalo, Andrea Osimani, Lucia Aquilanti, Cristiana Garofalo, Irena Budic-Leto
Summary: This study aimed to characterize the indigenous mycobiota of Marastina grape variety in Croatia. The researchers identified 25 different fungal genera on the grape berries from 11 vineyards located in different regions of Dalmatia. The distribution of fungal communities showed significant regional and local scale differences. The study also found that climate conditions, soil type, and physicochemical characteristics of fresh musts influenced the distribution of fungal communities. Understanding the indigenous mycobiota can provide valuable information for disease control and improving grapevine sanitary status.
FOOD RESEARCH INTERNATIONAL
(2022)
