Article
Oncology
Javier Narbona, Ruben G. Gordo, Jaime Tome-Amat, Javier Lacadena
Summary: Due to its high incidence and mortality, cancer is still a major risk to human health and lifespan. Immunotherapy and immunotoxins have emerged as promising approaches to overcome this global disease. This study designed two non-immunogenic variants of immunotoxins and demonstrated their enhanced antitumor activity and lower immunogenicity compared to the wild-type immunotoxin. These findings provide a step forward in optimizing immunotoxins for improved outcomes in cancer treatment.
Article
Biochemistry & Molecular Biology
Javier Narbona, Luisa Hernandez-Baraza, Ruben G. Gordo, Laura Sanz, Javier Lacadena
Summary: Immunotoxins are chimeric molecules that combine the specificity of an antibody-based targeting domain and the cytotoxic potency of a toxin. This study designed and characterized several immunotoxin constructs using nanobodies as targeting domains against EGFR. The results confirm the therapeutic potential of a-sarcin-based nanoITXs.
Article
Medicine, Research & Experimental
Jiang Chang, Xilin Liu, Honglin Ren, Shiying Lu, Meng Li, Song Zhang, Ke Zhao, Hanxiao Li, Xiaoshi Zhou, Lixiong Peng, Zengshan Liu, Pan Hu
Summary: The study developed a new version of CCK2R-targeting immunotoxin GD9P, which showed higher antitumor activity against human colorectal cancer compared to the parental immunotoxin, suggesting its promising potential for immunotherapy targeting CCK2R-expressing colorectal malignancies.
MOLECULAR PHARMACEUTICS
(2021)
Review
Biochemistry & Molecular Biology
Laura Sanz, Raquel Ibanez-Perez, Patricia Guerrero-Ochoa, Javier Lacadena, Alberto Anel
Summary: Monoclonal antibodies are an important treatment option for advanced colorectal cancer, but their clinical activity may be limited. One promising strategy to enhance their therapeutic potential is arming them with a toxic payload in the form of immunotoxins. However, optimizing immunotoxins faces challenges such as suboptimal pharmacokinetics and immunogenicity of the toxin portion. Ongoing research aims to address these limitations and expand the therapeutic arsenal for CRC patients.
Article
Immunology
Maryam Keshtvarz, Ehsan Rezaei, Jafar Amani, Mohammad Reza Pourmand, Jafar Salimian, Sheila Sarial, Masoumeh Douraghi
Summary: Immunotoxins, a type of targeted therapy, can kill cells using highly potent bacterial, fungal or plant toxins. In this study, a Stx-based immunotoxin was designed and used to selectively induce cytotoxicity and apoptosis in Fn-14-positive cells related to colon and lung cancer.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ni Shi, Xiaoxin Chen, Tong Chen
Summary: Colorectal cancer is a significant health burden worldwide, with nutrition and dietary factors known to have an impact on its development and prognosis. Fruits and vegetables rich in cancer-fighting phytochemicals, particularly anthocyanins, have been shown to have protective effects against cancer and other chronic diseases, with berries and grapes being common sources of anthocyanins with antitumor effects.
Article
Polymer Science
Oznur Akbal Vural, Yesim Tugce Yaman, Serdar Abaci
Summary: This study investigated the development of polydopamine nanoparticle as a drug carrier system for saponin and its use in targeted cancer therapy. The results showed that the system had high loading capacity and sustained release properties, and exhibited enhanced toxicity and cellular uptake compared to non-targeted nanoparticles.
JOURNAL OF APPLIED POLYMER SCIENCE
(2022)
Article
Oncology
Shuifa Wu, Cuimin Deng, Caiyun Zhang, Jiani Xiong, Xiaofan Gu, Ze Wang, Jingjing Tu, Jieming Xie
Summary: This study successfully constructed and highly purified a novel EGFR-specific immunotoxin C-CUS245C, which showed significant anti-tumor activity against colorectal cancer in vitro and in vivo. The cytotoxicity assays revealed that C-CUS245C had potent and specific efficacy on EGFR-positive cells, indicating its therapeutic potential for targeted therapy of colorectal neoplasms.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2021)
Article
Oncology
Daxiang Chen, Ruixue Wang, Mingjian Long, Wei Li, Bin Xiao, Hao Deng, Kongyan Weng, Daoyuan Gong, Fang Liu, Shuhong Luo, Wenbo Hao
Summary: This study demonstrated that ORFV strain NA1/11 can effectively inhibit the growth and migration of CRC cells both in vitro and in vivo, and regulates key factors relevant to apoptosis, autoimmunity/inflammation, angiogenesis, and the cell cycle. ORFV infection induces oncolytic activity by enhancing apoptosis, making it a potential therapeutic option for CRC.
Review
Pharmacology & Pharmacy
Silpita Paul, Dia Roy, Subhadip Pati, Gaurisankar Sa
Summary: Andrographis paniculata, a traditional medicinal herb from Chinese and Indian origin, has shown potential importance in colorectal cancer treatment, especially through its bioactive component andrographolide. Studies have highlighted its immunomodulatory effects and targeting of signaling pathways in the context of cancer. Further research, especially in vivo, is recommended to assess its potential as an effective candidate against colorectal cancer.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Medicine, Research & Experimental
Alba Ortigosa-Palomo, Cristina Porras-Alcala, Francisco Quinonero, Federico Moya-Utrera, Raul Ortiz, Juan M. Lopez-Romero, Consolacion Melguizo, Francisco Sarabia, Jose Prados
Summary: In this study, a chemically synthesized Bengamide II was shown to have potent antiproliferative activity in lung cancer cells and significant safety in in vivo models. It was found to inhibit cell proliferation, reduce tumor volume and metastases, and show no systemic toxicity at therapeutic doses. These findings suggest that Bengamide II is a promising drug for the treatment of lung cancer.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Medicine, Research & Experimental
Larissa Costa de Almeida, Jorge Antonio Elias Godoy Carlos, Paula Rezende-Teixeira, Joao Agosrinho Machado-Neto, Leticia Veras Costa-Lotufo
Summary: This study investigated the potential of the multikinase inhibitor AD80 as a drug option for colorectal cancer therapy. Results showed that AD80 selectively reduces cell viability of CRC cells by targeting the upregulated PI3K/AKT/mTOR and MAPK signaling pathways, suggesting AD80 as a promising candidate for colorectal cancer treatment by inhibiting tumor growth via multiple mechanisms, including clonogenicity inhibition, G(2)/M arrest, DNA damage, apoptosis, and inhibition of S6RP phosphorylation.
Article
Chemistry, Multidisciplinary
Zhenzhen Chen, Luyun He, Liangbo Zhao, Guangtan Zhang, Zhiwei Wang, Pingping Zhu, Benyu Liu
Summary: Colorectal cancer (CRC) is a common tumor worldwide, and circular RNA circREEP3 is found to be upregulated in CRC tissues. Upregulation of circREEP3 predicts poor patient survival. Mechanistically, circREEP3 activates FKBP10 transcription to promote CRC tumorigenesis and metastasis, while restricting RIG-1-dependent antitumor immunity.
Article
Pharmacology & Pharmacy
Renata S. Fernandes, Raquel Gregorio Arribada, Juliana O. Silva, Armando Silva-Cunha, Danyelle M. Townsend, Lucas A. M. Ferreira, Andre L. B. Barros
Summary: This study developed a novel hybrid nanoparticle for the treatment of breast cancer by combining the advantages of liposomes and polymeric nanoparticles. The nanoparticles demonstrated significant reductions in cell viability and migration, as well as enhanced cellular uptake, leading to effective inhibition of tumor growth. The increased accumulation of drugs in the tumor area, along with the synergic combination with TS, further improved the antitumor efficacy.
Article
Chemistry, Multidisciplinary
Long Xiao, Yu Huang, Yuhe Yang, Zhiwei Miao, Jie Zhu, Mengdan Zhong, Chencheng Feng, Wenkai Tang, Jinhua Zhou, Lihong Wang, Xin Zhao, Zhirong Wang
Summary: The researchers have developed a biomimetic cytomembrane cancer nanovaccine named CCMP@R837, which enhanced the immune response against breast cancer cells and established immune memory in BALB/c mice. This vaccine platform shows potential as a candidate for preventive cancer vaccines in clinical settings.
Article
Pediatrics
Carlos Delgado-Miguel, Antonio Munoz-Serrano, Maria San Basilio, Miriam Miguel-Ferrero, Maria de Ceano-Vivas, Leopoldo Martinez
Summary: The study examined the role of the NLR in appendectomy and compared its usefulness with other clinical factors. The NLR was found to be the best preoperative parameter for distinguishing patients without acute appendicitis. Clinicians should consider alternative diagnoses when the NLR is less than 2.65.
ANALES DE PEDIATRIA
(2023)
Article
Dermatology
Carlos Delgado-Miguel, Antonio J. Munoz-Serrano, Lucas Moratilla, Maria del Carmen Sarmiento, Miriam Miguel-Ferrero, Leopoldo Martinez
Summary: This study aimed to compare the effectiveness of silver nitrate and electrocautery in the treatment of stage II-III ingrown toenails in adolescents. The results showed that silver nitrate chemical matrixectomy had a lower recurrence rate and postoperative infection rate, suggesting it as a possible alternative to electrocautery matricectomy.
PEDIATRIC DERMATOLOGY
(2023)
Review
Pediatrics
Eva Sorensen, Connie Yu, Shu-Ling Chuang, Paola Midrio, Leopoldo Martinez, Mathew Nash, Ingo Jester, Amulya K. K. Saxena
Summary: The aim of this multicenter retrospective study and literature review was to review management and outcomes of neonatal esophageal perforation (NEP). The study found that NEP during NGT insertion is rare even in premature infants. In this small cohort, conservative management seems to be safe. A larger sample size will be necessary to answer questions on efficacy of antibiotics, antacids and NGT re-insertion time frame in NEP.
Article
Biochemistry & Molecular Biology
Maria Villa-Morales, Laura Perez-Gomez, Eduardo Perez-Gomez, Pilar Lopez-Nieva, Pablo Fernandez-Navarro, Javier Santos
Summary: The standard-of-care treatment for T-cell acute lymphoblastic leukaemia (T-ALL) achieves reasonable response rates, but relapsed or treatment-resistant patients have poor outcomes. This study explores the potential of NRF2 activation as a prognostic biomarker in T-ALL. Through analysis of transcriptomic, genomic, and clinical data, high NFE2L2 levels were found to be associated with shorter overall survival in T-ALL patients. The PI3K-AKT-mTOR pathway plays a role in the oncogenic signaling induced by NRF2 in T-ALL. High NFE2L2 levels are also associated with drug resistance in T-ALL, possibly due to NRF2-induced glutathione biosynthesis. These findings suggest that NFE2L2 levels could be a predictive biomarker for poor treatment response in T-ALL patients, offering insights for improved patient stratification and targeted therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pediatrics
Cunera M. C. de Beaufort, Dalia Aminoff, Ivo de Blaauw, Celia Cretolle, Jens Dingemann, Natalie Durkin, Wout F. J. Feitz, JoAnne Fruithof, Caterina Grano, Carmen Mesas Burgos, Nicole Schwarzer, Graham Slater, Tutku Soyer, Cristiano Violani, Rene Wijnen, Paolo de Coppi, Ramon R. Gorter
Summary: The aim of this international study was to assess the current status of transition of care (TOC) and adult care (AC) programs for patients with anorectal malformations (ARM) and Hirschsprung disease (HD). The results showed that less than 50% of the participating centers had installed these programs, and there was considerable heterogeneity in the content and implementation of the programs. Sharing best practice examples and considering local and national healthcare programs may improve the continuity of care in the future.
JOURNAL OF PEDIATRIC SURGERY
(2023)
Article
Oncology
Javier Narbona, Ruben G. Gordo, Jaime Tome-Amat, Javier Lacadena
Summary: Due to its high incidence and mortality, cancer is still a major risk to human health and lifespan. Immunotherapy and immunotoxins have emerged as promising approaches to overcome this global disease. This study designed two non-immunogenic variants of immunotoxins and demonstrated their enhanced antitumor activity and lower immunogenicity compared to the wild-type immunotoxin. These findings provide a step forward in optimizing immunotoxins for improved outcomes in cancer treatment.
Article
Oncology
Carlos Costas-Insua, Marta Seijo-Vila, Cristina Blazquez, Sandra Blasco-Benito, Francisco Javier Rodriguez-Baena, Giovanni Marsicano, Eduardo Perez-Gomez, Cristina Sanchez, Berta Sanchez-Laorden, Manuel Guzman
Summary: Based on in vitro and in silico experiments, it has been discovered that the cannabinoid CB1 receptor regulates the proliferation of melanoma brain metastases. This finding provides a new perspective for further research on melanoma brain metastases.
Article
Biochemistry & Molecular Biology
Vicente Andreu-Fernandez, Mariona Serra-Delgado, Laura Almeida-Toledano, Agueda Garcia-Meseguer, Melina Vieiros, Anna Ramos-Triguero, Concha Munoz-Lozano, Elisabet Navarro-Tapia, Leopoldo Martinez, Oscar Garcia-Algar, Maria D. Gomez-Roig
Summary: Prenatal alcohol exposure can cause cardiovascular health issues in offspring. This study investigated the effects of the compound EGCG on cardiac dysfunction in mice prenatally exposed to alcohol. The results showed that prenatal alcohol exposure led to cardiac alterations and dysfunction, but postnatal treatment with EGCG restored physiological levels of heart biomarkers and improved cardiac function. These findings suggest that postnatal EGCG treatment can attenuate the damage caused by prenatal alcohol exposure.
Article
Pediatrics
Carlos Delgado-Miguel, Antonio J. Munoz-Serrano, Lucas Moratilla, Miriam Miguel-Ferrero, Bonifacio Delgado, Juan Camps, Manuel Lopez-Santamaria, Leopoldo Martinez
Summary: This study aimed to explore the role of neutrophil-to-lymphocyte ratio (NLR) as a predictor of surgical site infection (SSI) after appendectomy in children. The results showed that NLR value at admission was the most promising predictive factor for SSI in children undergoing appendectomy. It is an easy, simple, inexpensive, and rapid method to detect patients at high risk for SSI.
TRANSLATIONAL PEDIATRICS
(2023)
Article
Allergy
Jaime Tome-Amat, Zulema Gonzalez-Klein, Diego Pazos-Castro, Jose Luis Resuela-Gonzalez, Gonzalo Vilchez-Pinto, Helena Berreiros-Hortala, Lucia Palacio-Garcia, Sergio Fernandez-Bravo, Vanesa Esteban, Araceli Diaz-Perales, Maria Garrido-Arandia
Article
Multidisciplinary Sciences
Isabel Tundidor, Marta Seijo-Vila, Sandra Blasco-Benito, Maria Rubert-Hernandez, Sandra Adamez, Clara Andradas, Sara Manzano, Isabel Alvarez-Lopez, Cristina Sarasqueta, Maria Villa-Morales, Carmen Gonzalez-Lois, Esther Ramirez-Medina, Belen Almoguera, Antonio J. Sanchez-Lopez, Laura Bindila, Sigrid Hamann, Norbert Arnold, Christoph Roecken, Ignacio Heras-Murillo, David Sancho, Gema Moreno-Bueno, Maria M. Caffarel, Manuel Guzman, Cristina Sanchez, Eduardo Perez-Gomez
Summary: Clinical management of breast cancer metastasis is an unmet need. This study identifies fatty acid amide hydrolase (FAAH) as a predictor of prognosis in patients with luminal BC and a potential therapeutic target for preventing tumor progression and lung metastasis.
NATURE COMMUNICATIONS
(2023)
Article
Surgery
Carlos Delgado-Miguel, Miriam Miguel-Ferrero, Antonella Garcia, Bonifacio Delgado, Juan Camps, Leopoldo Martinez
Summary: This study aimed to analyze the role of neutrophil-to-lymphocyte ratio (NLR) in predicting postoperative complications and readmission in children after appendectomy. The results showed that NLR at admission is a reliable indicator for identifying high-risk surgical patients and predicting postoperative complications and readmission in children with acute appendicitis
UPDATES IN SURGERY
(2023)
Article
Pediatrics
Mar Romero-Lopez, Marc Oria, Fernando Ferrer-Marquez, Maria Florencia Varela, Kristin Lampe, Miki Watanabe-Chailland, Leopoldo Martinez, Jose L. L. Peiro
Summary: A study using the rat nitrofen model suggests that chronic hypoxia and imbalanced energy production could contribute to the pathogenesis of congenital diaphragmatic hernia. Novel therapies targeting mitochondria might improve outcomes.
PEDIATRIC SURGERY INTERNATIONAL
(2023)
Article
Pediatrics
Maria San Basilio, Carlos Delgado-Miguel, Carla Ramirez-Amoros, Maria Sarmiento, Lucas Moratilla-Lapena, Arturo Almeyda, Ricardo Mejia, Leopoldo Martinez
Summary: This study aimed to investigate the necessity of nighttime appendectomy and found that the timing of surgery does not affect postoperative complications.
PEDIATRIC SURGERY INTERNATIONAL
(2023)