Journal
JOURNAL OF SYNCHROTRON RADIATION
Volume 21, Issue -, Pages 1370-1377Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1600577514015033
Keywords
cell biology; cellular imaging; cryo-preservation; cryogenic fluorescence tomography; cryostage; three-dimensional reconstruction
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Funding
- US Department of Energy, Office of Basic Energy Science [DE-AC02-05CH11231]
- US Department of Energy, Office of Biological and Environmental Research [DE-AC02-05CH11231]
- National Center for Research Resources of the National Institutes of Health [P41RR019664]
- National Institute of General Medicine of the National Institutes of Health [P41GM103445]
- Gordon and Betty Moore Foundation [3497]
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Beamline 2.1 (XM-2) is a transmission soft X-ray microscope in sector 2 of the Advanced Light Source at Lawrence Berkeley National Laboratory. XM-2 was designed, built and is now operated by the National Center for X-ray Tomography as a National Institutes of Health Biomedical Technology Research Resource. XM-2 is equipped with a cryogenic rotation stage to enable tomographic data collection from cryo-preserved cells, including large mammalian cells. During data collection the specimen is illuminated with 'water window' X-rays (284-543 eV). Illuminating photons are attenuated an order of magnitude more strongly by biomolecules than by water. Consequently, differences in molecular composition generate quantitative contrast in images of the specimen. Soft X-ray tomography is an information-rich three-dimensional imaging method that can be applied either as a standalone technique or as a component modality in correlative imaging studies.
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