Article
Pharmacology & Pharmacy
Nicolas Araya, Martin A. Leiva-Soto, Maria V. V. Bruna, Almendra Castro-Munoz, Beatriz Behrend-Keim, Daniel Moraga-Espinoza, Tania F. F. Bahamondez-Canas
Summary: This study aimed to enhance the solubility of Buddleja globosa extract by spray drying with solubility enhancers. The spray-dried extract showed improved antimicrobial properties and inhibition of biofilm formation, indicating its potential for further research and development of phytopharmaceuticals.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Jing Yang, Qing-Ming Qin, Paul de Figueiredo
Summary: This article introduces a newly developed automated fluorescence microscopy-based imaging method, combined with high-throughput image processing and statistical analysis, which can rapidly and accurately quantify bacterial adherence to host cells. Experimental results demonstrate that this method significantly reduces experimental workloads and timelines.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Letter
Biochemical Research Methods
Anming Xu, Xiaoxiao Zhang, Shixiang Cao, Xiaoli Zhou, Ziyi Yu, Xiujuan Qian, Jie Zhou, Weiliang Dong, Min Jiang
Summary: In this study, a novel transcription-associated fluorescence-activated droplet sorting method was developed for specific targeting of di-RL hyperproducers. Using this method, ten higher di-RL producing microorganisms were screened out, which showed significantly increased di-RL yield compared to the model strain. This study provides a rapid, reliable screening method for di-RL production and offers a way to measure di-RL yield in populations of droplets containing different environmental colonies.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Agricultural Engineering
Qian Feng, Laiwei Luo, Xindi Chen, Kaijie Zhang, Fang Fang, Zhaoxia Xue, Chao Li, Jiashun Cao, Jingyang Luo
Summary: Exogenous AHLs such as C6-HSL and C8-HSL were found to promote biofilm formation of Pseudomonas aeruginosa significantly, enhancing adhesive ability, bacterial motility, and metabolic activities. These AHLs also upregulated the expression of microbial functional genes involved in biofilm formation, providing valuable insights for biofilm regulation in wastewater treatment using biofilm technology.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biology
Hala Tamim El Jarkass, Calvin Mok, Michael R. Schertzberg, Andrew G. Fraser, Emily R. Troemel, Aaron W. Reinke
Summary: This study identified a secreted protein used by microsporidia to promote host invasion and found that this intestinal protein plays a crucial role in the infection of microsporidia. The results also suggest that there may be evolutionary trade-offs in host defense against multiple classes of pathogens.
Article
Materials Science, Coatings & Films
Xin Cheng, Jiashun Shi, Weigang Wang, Huimin Liao, Shiqiang Chen, Guangzhou Liu, Jvna Chen
Summary: Biofilm formation is a major cause of microbiologically influenced corrosion, and inhibiting microbial adhesion is an effective method to control biofilm formation. Anodization of EH40 steel in NaOH solution can create functional films that inhibit the adhesion of P. aeruginosa, with the best anti-adhesion performance achieved under visible light irradiation due to hydroxyl radicals generated by the nanostructured oxide film.
SURFACE & COATINGS TECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Mahere Momenijavid, Himen Salimizand, Aazam Korani, Omid Dianat, Bijan Nouri, Rashid Ramazanzadeh, Amjad Ahmadi, Jino Rostamipour, Mohammad Rastegar Khosravi
Summary: This study evaluated the effects of calcium hydroxide on the morphology and physicochemical properties of Enterococcus faecalis biofilm. It was found that calcium hydroxide could contribute to a denser biofilm structure with more cavities and a granular surface, resulting in increased biomass, thickness, colony size, and volume of the biofilm.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Li Li, Zuxian Xu, Ruipin Cao, Jiaxin Li, Chang-Jer Wu, Yinglu Wang, Hu Zhu
Summary: We investigated the influence of hydroxyl groups on the anti-quorum-sensing (anti-QS) and anti-biofilm activity of cyclic dipeptides against Pseudomonas aeruginosa PAO1. The presence of hydroxyl groups improved the inhibition of virulence factors and cytotoxicity, but reduced the ability to inhibit biofilm formation. These cyclic dipeptides interacted with the QS-related protein LasR, and the introduction of hydroxyl groups enhanced their self-assembly ability.
Article
Medicine, Research & Experimental
Yuanze Gao, Helin Chen, Wen Li, Yu Zhang, Jie Luo, Ling Zhao, Fei Shi, Gang Ye, Xiaoli He, Zhiwen Xu, Ling Zhu, Huaqiao Tang, Yinglun Li
Summary: This study evaluated the effect of chloroform extracts from Atractylodes chinensis on the virulence of Salmonella Typhimurium (STM) and found that it could attenuate the adhesion and invasion abilities of STM, as well as inhibit the expression of genes related to adhesion and invasion. It also downregulated the expression of inflammatory cytokines and restored the expression of intestinal barrier-related genes and proteins damaged during STM infection.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Biotechnology & Applied Microbiology
Geum-Jae Jeong, Fazlurrahman Khan, Sohail Khan, Nazia Tabassum, Sonu Mehta, Young-Mog Kim
Summary: Pseudmonas aeruginosa is a widespread Gram-negative bacterium responsible for infections in various parts of the body. The development of multiple resistance mechanisms has led to failure of antimicrobial therapy. Inhibition of siderophore production in P. aeruginosa has emerged as a promising therapeutic option to limit their infection.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Editorial Material
Genetics & Heredity
Tatyana L. Povolotsky, Alona Keren-Paz, Ilana Kolodkin-Gal
Summary: For bacteria, the transition from unicellular entities to multicellular biofilm communities generates distinct metabolic microenvironments. Dynamic and programmed metabolic responses allow the biofilms to react to local changes in nutrient levels and contribute to phenotypic antibiotic resistance of the community. Novel therapeutic approaches targeting biofilms may stem from these metabolic adaptations.
TRENDS IN GENETICS
(2021)
Article
Microbiology
Zhidong Zhang, Yang Sun, Yuanyang Yi, Xiaoyu Bai, Liying Zhu, Jing Zhu, Meiying Gu, Yanlei Zhu, Ling Jiang
Summary: A strain of Streptomyces with quorum-sensing inhibitory activity was isolated from the Kumutag Desert in Xinjiang, and it effectively inhibited biofilm formation and virulence factor production in bacteria such as Pseudomonas aeruginosa.
Article
Plant Sciences
Shimaa El-Sapagh, Rania El-Shenody, Leonel Pereira, Mostafa Elshobary
Summary: This study investigated the biofilm activity and antibiotic resistance profiles of Pseudomonas aeruginosa isolates from a fish-rearing farm, as well as the potential of algal extracts as antibacterial and antibiofilm agents. The study found that the algal extracts showed promising effects against Pseudomonas aeruginosa, particularly in reducing the expression of biofilm-related genes. Specific compounds in the extracts were found to contribute to their activities.
Article
Biochemistry & Molecular Biology
Marco Artini, Gianluca Vrenna, Marika Trecca, Vanessa Tuccio Guarna Assanti, Ersilia Vita Fiscarelli, Rosanna Papa, Laura Selan
Summary: Pseudomonas aeruginosa is a bacterium frequently involved in cystic fibrosis airway infections. It increases its virulence through biofilm, motility, toxin production, and invasion of host cells. The metalloprotease serratiopeptidase (SPEP) has been shown to impair the virulence-related properties of Gram-positive bacteria. In this study, the effects of SPEP on different physiological aspects related to the virulence of P. aeruginosa isolated from CF patients were investigated. The results showed that SPEP impaired attachment to inert surfaces and adhesion/invasion of eukaryotic cells. It also had strain-dependent effects on pyocyanin and pyoverdine production. Additionally, SPEP seemed to increase swarming motility and staphylolytic protease production. Further studies are needed to understand why different strains react differently to specific treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Materials Science, Multidisciplinary
Jair Carrazco-Palafox, Blanca Estela Rivera-Chavira, Jaime Raul Adame-Gallegos, Luz Maria Rodriguez-Valdez, Erasmo Orrantia-Borunda, Guadalupe Virginia Nevarez-Moorillon
Summary: The study evaluated the efficacy of rhamnolipids from Pseudomonas aeruginosa Rn19a in inhibiting biofilm formation by clinical isolates of Escherichia coli, Pseudomonas aeruginosa, and Enterococcus faecalis. Results showed that rhamnolipids effectively reduced biofilm formation in static and dynamic conditions, with different levels of effectiveness against different bacterial isolates. The application of rhamnolipids may be useful in preventing initial bacterial adhesion to surfaces.
Article
Biochemistry & Molecular Biology
Raghda Abdelnasser Singab, Nooran Sherif Elleboudy, Walid Faisal Elkhatib, Mahmoud Abdulmegead Yassein, Nadia Adelhaleem Hassouna
Summary: This study achieved a significant increase in PHBA production through biotransformation of caffeic acid, optimizing the process through mutation and response surface methodology modeling. The immobilized CI-24 mutant cells in calcium alginate beads retained equivalent biotransformation capacity after three successive cycles, enhancing process performance and economic feasibility.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Chemistry, Inorganic & Nuclear
Mohamed S. Attia, Naglaa M. Balabel, Ibtisam M. Ababutain, Mahmoud S. Osman, Mohamed M. Nofel, M. Abd Elkodous, Walid F. Elkhatib, Gharieb S. El-Sayyad, Ahmed I. El-Batal
Summary: The study evaluated the potential impact of CuO NPs-streptomycin nano-drug on potato brown rot disease, with results indicating that treatment with CuO NPs during planting was more effective.
JOURNAL OF CLUSTER SCIENCE
(2022)
Article
Multidisciplinary Sciences
Samira M. Hamed, Walid F. Elkhatib, Ahmed S. Khairalla, Ayman M. Noreddin
Summary: The global spread of COVID-19 has led to increased interest in studying the genomic variations of the novel SARS-CoV-2 virus. Analysis of metadata from over 400,000 SARS-CoV-2 genomes revealed differences in distribution of genomic clades based on geographic locations, age groups, and genders. Over time, there has been an expansion of clades with D614G mutations, with the newest clade GV becoming predominant in the last three months. These findings highlight the evolution of SARS-CoV-2 and its impact on infectivity, virulence, and mortality.
SCIENTIFIC REPORTS
(2021)
Article
Architecture
Doha M. Saeed, Walid F. Elkhatib, Ahmed M. Selim
Summary: The study aims to address the challenges in classroom design by reducing COVID-19 infections and improving the health and performance of users through an electronic questionnaire and computational fluid dynamics (CFD) model. In addition, four alternative classroom designs were proposed based on the interpretive results from the questionnaire and simulation.
JOURNAL OF ASIAN ARCHITECTURE AND BUILDING ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Masarra M. Sakr, Walid F. Elkhatib, Khaled M. Aboshanab, Eman M. Mantawy, Mahmoud A. Yassien, Nadia A. Hassouna
Summary: The research found that using recombinant Ahl-1 lactonase hydrogel can effectively control multidrug resistant P. aeruginosa infections, leading to enhanced healing and reduced mortality rate.
Article
Chemistry, Inorganic & Nuclear
Gharieb S. El-Sayyad, M. I. A. Abdel Maksoud, Ramy Amer Fahim, Mohamad Bekhit, Said M. Kassem, A. S. Awed, Mohamed Gobara, Ahmed El-Batal, A. H. Ashour, Walid F. Elkhatib, Salim Mohamed Abd El-Aziz
Summary: In this study, a chitosan/gold/bioactive glass nanocomposite was successfully synthesized and its antimicrobial efficacy and antibiofilm properties were evaluated. The results showed that CS/Au/BG exhibited good antimicrobial activity and could be a potential candidate for combating pathogenic microbes in wastewater treatment.
JOURNAL OF CLUSTER SCIENCE
(2023)
Article
Immunology
Asmaa Gohar, Aya A. Ali, Walid F. Elkhatib, Gharieb S. El-Sayyad, Dounia Elfadil, Ayman M. Noreddin
Summary: HPV, the most prevalent sexually transmitted disease, can potentially be prevented and treated effectively using nanotechnology-based vaccines that increase efficacy and reduce side effects.
MICROBIAL PATHOGENESIS
(2022)
Review
Virology
Walid F. Elkhatib, Shereen S. Abdelkareem, Wafaa S. Khalaf, Mona Shahin, Dounia Elfadil, Alaa Alhazmi, Ahmed El-Batal, Gharieb S. El-Sayyad
Summary: This report reviews the history of pandemics, focusing on COVID-19, and provides insights into the origin, transmission modes, causative factors, and control measures for respiratory viral diseases. Nanotechnology and its potential applications in COVID-19 treatment and immune system enhancement are also investigated.
Article
Engineering, Electrical & Electronic
Shanmugam Akilandeswari, Lakshmanan Jothi, Walid F. Elkhatib, Eman Fayad, Ola A. Abu Ali, Gharieb S. El-Sayyad
Summary: This study investigates the doping effect of oxalic acid on proline single-crystal through a slow evaporation procedure. XRD analysis confirms the crystal structure of proline oxalate, while FTIR and FT-Raman spectroscopy show frequency shifting due to oxalic acid supply. SEM examines the morphology of the single-crystal. Optical properties are characterized by UV-NIR light transmission and fluorescence examination. The material shows a significant frequency shift in SHG compared to KDP. Thermal properties are studied using TGA, DTA, and DCS, revealing a melting point of 185 degrees C.
OPTICAL AND QUANTUM ELECTRONICS
(2023)
Article
Immunology
Ahmed I. El-Batal, Gharieb S. El-Sayyad, Bassam M. Al-shammari, Amer M. Abdelaziz, Mohamed M. Nofel, Mohamed Gobara, Walid F. Elkhatib, Nerhan A. Eid, Marwa S. Salem, Mohamed S. Attia
Summary: Recently, nanocomposites have been widely used as growth promoters and antifungal agents for plants. A new technique was employed to fabricate Fe2O3 nanocomposites conjugated with humic acid (H) and boron (B) using gamma-rays as a reducing agent. The antifungal potential of these nanocomposites against Fusarium oxysporum, the causative agent of cucumber wilt disease, was evaluated in vitro and in vivo.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Rania I. Shebl, Walid F. Elkhatib, Mona Shaban E. M. Badawy
Summary: We investigated the interaction between zinc sulfate and antibiotics against Klebsiella pneumoniae biofilm. The results showed that zinc enhanced the antibiofilm activity of cephalosporins and aminoglycosides, but antagonized the effectiveness of fluoroquinolones and meropenem. Zinc down-regulated the expression of biofilm-related genes, thus enhancing the antibiofilm efficacy of cephalosporins and aminoglycosides.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2023)
Article
Microbiology
Mona Shaban E. M. Badawy, Walid F. Elkhatib, Rania I. Shebl
Summary: This study evaluated the anti-biofilm potentials of various antibiotics alone and in combination against biofilm-forming carbapenem-resistant Pseudomonas aeruginosa (CRPA). The results showed that colistin exhibited the strongest anti-biofilm activity, followed by gentamicin and meropenem, while ceftazidime had the least activity. Combination therapy showed synergistic effects. The simulated pharmacodynamic modeling confirmed the synergistic anti-biofilm activity observed in vitro.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2023)
Article
Chemistry, Multidisciplinary
Mennatallah A. Mohamed, Maha Nasr, Walid F. Elkhatib, Wafaa N. Eltayeb, Aliaa A. Elshamy, Gharieb S. El-Sayyad
Summary: This study evaluated the antimicrobial activity and PAEs of nanobiotic formulations against MRSA. The results showed that nanobiotics significantly increased the susceptibility of MRSA isolates and prolonged the PAE values compared to conventional antibiotics.
Review
Chemistry, Multidisciplinary
Mohamed Mohamady Ghobashy, Mohamed Abd Elkodous, Soha Hamdy Shabaka, Sherif A. Younis, Dalal Mohamed Alshangiti, Mohamed Madani, Samera Ali Al-Gahtany, Walid F. Elkhatib, Ayman M. Noreddin, Norhan Nady, Gharieb S. El-Sayyad
Summary: This review delves into the extensive use of silver nanoparticles in various industries and the potential implications they have on humans and the environment. It discusses aspects such as production, detection techniques, fate, and dynamics of AgNPs under different environmental factors. Furthermore, the toxicity mechanism and interactions of AgNPs with different matrices are highlighted, including aquatic environments, soils, crops, and humans, with discussions on reduction measures and future research directions.
NANOTECHNOLOGY REVIEWS
(2021)
Article
Public, Environmental & Occupational Health
Rodrigo C. Menezes, Isabella B. B. Ferreira, Luciana Sobral, Stefania L. Garcia, Hugo N. Pustilnik, Mariana Araujo-Pereira, Bruno B. Andrade
Summary: This study aimed to identify the clinical features associated with viral pathogens responsible for severe lower respiratory tract infections (LRTI) in children. The study found that different viral agents have distinct associations with clinical features in children.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2024)
Article
Public, Environmental & Occupational Health
Ambrina Khatoon, Syed F. Hussain, Syed M. Shahid, Santosh Kumar Sidhwani, Salman Ahmed Khan, Omer Ahmed Shaikh, Abdulqadir J. Nashwan
Summary: Despite the increasing incidence of Staphylococcus aureus infection and dissemination in Pakistan, research on the epidemiology of different Staphylococcus aureus clones has been limited. This study used multilocus sequence typing (MLST) to analyze the epidemiology of Staphylococcus aureus in the area, finding high diversity of locally circulating clones defined by their geographic epidemiology.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2024)
Review
Public, Environmental & Occupational Health
Amir Khorram-Manesh, Krzysztof Goniewicz, Frederick M. Burkle Jr
Summary: This article discusses the management approach for globalized diseases in a globalized world. Through literature review and analysis, key focuses including data-driven decision-making, robust technology infrastructure, global cooperation, and ongoing public health education are identified. The weaknesses of current pandemic management systems are revealed, and recommendations for strengthening future pandemic management are provided.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2024)
Article
Public, Environmental & Occupational Health
Mst S. Munira, Yuta Okada, Hiroshi Nishiura
Summary: This study estimates the life expectancy at birth in Japan at the end of 2022 using death datasets from Aichi and Fukui prefectures. The results suggest that the impact of the pandemic on life expectancy was relatively small by the end of 2022.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2024)