Article
Biochemistry & Molecular Biology
Lina Abou Saleh, Abigail Boyd, Ileana Aragon, Anna Koloteva, Domenico Spadafora, Wadad Mneimneh, Robert A. Barrington, Wito Richter
Summary: Genetic ablation of PDE4B protects mice from acute lung injury induced by P aeruginosa infection by reducing inflammation, vascular leakage, and mortality, while improving bacterial clearance. This demonstrates the potential therapeutic target of PDE4B in P aeruginosa lung infections.
Article
Microbiology
Nina S. Baggett, Adam S. Bronson, Matthew T. Cabeen
Summary: Pyocins are used by Pseudomonas aeruginosa to compete with other strains, typically induced by DNA damage and the SOS response. Our discovery of a RecA-independent pathway for strong pyocin induction in XerC-deficient cells highlights a potential target for future therapies against P. aeruginosa infections.
Article
Microbiology
Hongdong Li, Xingyuan Li, Qing Ai, Liping Tan
Summary: This study found that the quorum sensing signal autoinducer-2 (AI-2) promotes acute lung infection caused by Pseudomonas aeruginosa through interfering with the quorum sensing system and the interleukin-17A (IL-17A) pathway.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Obinna Markraphael Ajunwa, Olubusola Ayoola Odeniyi, Emmanuel Oluwaseun Garuba, Mrinalini Nair, Enrico Marsili, Abiodun Anthony Onilude
Summary: This study determined the production of pyocyanin, NAD, and NAD synthetase in the electrogenic bacterium Pseudomonas aeruginosa using a Microbial Fuel Cell (MFC). The co-expression of nadD and nadC genes increased the activity of NAD synthetase and resulted in higher pyocyanin concentration, NAD/NADH levels, and MFC potential in PA-A4 strain. This finding advances our understanding of NAD biosynthetic genes in PA electrogenicity.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ke-Qin Gong, Carmen Mikacenic, Matthew E. Long, Charles W. Frevert, Timothy P. Birkland, Jean Charron, Sina A. Gharib, Anne M. Manicone
Summary: Inhibition of MAP2K2 can improve acute lung injury (ALI) and deficiency of MAP2K2 can accelerate the resolution of ALI. Genetic variants in MAP2K2 are associated with mortality from acute respiratory distress syndrome (ARDS). MAP2K2 plays an important role in promoting and sustaining proinflammatory pathway activation in ALI.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Microbiology
Baopeng Yang, Yujun Jiang, Yongxin Jin, Fang Bai, Zhihui Cheng, Weihui Wu
Summary: Polymyxins are last-resort antibiotics for treating infections caused by multidrug-resistant Gram-negative pathogens. Pseudomonas aeruginosa, an opportunistic pathogen, has proteins that contribute to bacterial resistance to polymyxins by modifying lipopolysaccharides and maintaining membrane integrities. The study found that a mutation in the oligoribonuclease gene increased bacterial survival after polymyxin B treatment in P. aeruginosa. Pel, a protein that binds to extracellular DNA, was also shown to trap polymyxin B and protect bacterial cells.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Review
Biotechnology & Applied Microbiology
Ahmed A. Abdelaziz, Amal M. Abo Kamer, Khaled B. Al-Monofy, Lamiaa A. Al-Madboly
Summary: The discussion focuses on the bioprospecting of microorganisms and their bioactive byproducts, specifically pigments. Microbial pigments, such as pyocyanin produced by Pseudomonas aeruginosa, have various benefits including their natural composition, therapeutic effects, and year-round production. This article concentrates on the production, extraction, and biological applications of pyocyanin pigment in biotechnology, engineering, and biology fields.
MICROBIAL CELL FACTORIES
(2023)
Article
Pharmacology & Pharmacy
Sunil Kumar Bose, Karuna Sharma, Sanjay Chhibber, Kusum Harjai
Summary: The study demonstrated that the use of nanolipoidal alpha-terpineol could effectively treat Pseudomonas aeruginosa-induced keratitis, showing significant improvements in bacterial count, corneal pathology, and immunomodulatory effects.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Immunology
Lu-lu Li, Ying-gang Zhu, Xin-ming Jia, Dong Liu, Jie-ming Qu
Summary: ASCs treatment significantly reduced bacterial load, lung inflammation, and histopathological damage caused by PA. PA mainly activated NLRC4 inflammasome in the lungs of mice, while ASCs attenuated acute lung infection by inhibiting NLRC4 inflammasome activation. NLRC4(-/-) mice showed improved survival rate and lung bacterial load after PA infection, indicating the potential role of ASCs in treating acute pulmonary infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Nina Erdmann, Theresa Schilling, Julia Hentschel, Thomas Lehmann, Philipp von Bismarck, Tobias Ankermann, Franziska Duckstein, Michael Baier, Carlos Zagoya, Jochen G. Mainz
Summary: This study compared upper airway inflammation and pathogen colonization during stable phases and exacerbations in CF patients and healthy controls. The study found that CF patients had weaker inflammatory responses compared to healthy controls during both stable and exacerbation phases.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Agronomy
Gaber Attia Abo-Zaid, Ahmed Salah Abdullah, Nadia Abdel-Mohsen Soliman, Ebaa Ebrahim El-Sharouny, Abdulaziz A. Al-Askar, Yiming Su, Ahmed Abdelkhalek, Soraya Abdel-Fattah Sabry
Summary: The secretion of siderophores by Pseudomonas aeruginosa F2 and P. fluorescens JY3 was evaluated and found to have an inhibitory effect on Fusarium oxysporum and Rhizoctonia solani. Two optimized media were used to produce siderophores as biocontrol agents, and the cell-free supernatants containing siderophores were used to prepare bio-friendly formulations. These formulations showed significant reduction in damping-off caused by F. oxysporum and R. solani, as well as promoting the growth of wheat plants.
Article
Biotechnology & Applied Microbiology
Adilson Jose da Silva, Josivan de Souza Cunha, Teri Hreha, Kelli Cristina Micocci, Heloisa Sobreiro Selistre-de-Araujo, Blanca Barquera, Mattheos A. G. Koffas
Summary: This study engineered the pyocyanin biosynthetic pathway for high production in a heterologous host for the first time. Optimized culture and induction parameters led to a 3.5-fold increase in pyocyanin accumulation, while pathway balancing through testing plasmids with different copy numbers resulted in a fourfold difference in product titer among the engineered strains. Co-expression of Vitreoscilla hemoglobin Vhb further improved pyocyanin production to a final titer of 18.8 mg/L, demonstrating the potential of using Escherichia coli for phenazines production in electro-fermentation systems.
METABOLIC ENGINEERING
(2021)
Article
Multidisciplinary Sciences
Biljana Mojsoska, Melanie Ghoul, Gabriel G. Perron, Havard Jenssen, Fatima AlZahra'a Alatraktchi
Summary: This study explores the impact of sub-inhibitory antibiotic concentrations on the production of virulence factors in environmental Pseudomonas aeruginosa strains, showing that sub-inhibitory antibiotic concentrations can alter bacterial toxin production, with strain-specific effects. Additionally, biologically relevant measured toxin concentrations were found to decrease the viability of cultured epithelial cells. This suggests that antibiotic-induced virulence should be considered in routine pathogen testing.
Article
Multidisciplinary Sciences
Li Li, Zuxian Xu, Ruipin Cao, Jiaxin Li, Chang-Jer Wu, Yinglu Wang, Hu Zhu
Summary: We investigated the influence of hydroxyl groups on the anti-quorum-sensing (anti-QS) and anti-biofilm activity of cyclic dipeptides against Pseudomonas aeruginosa PAO1. The presence of hydroxyl groups improved the inhibition of virulence factors and cytotoxicity, but reduced the ability to inhibit biofilm formation. These cyclic dipeptides interacted with the QS-related protein LasR, and the introduction of hydroxyl groups enhanced their self-assembly ability.
Article
Microbiology
Xuerui Bao, Ellen Goeteyn, Aurelie Crabbe, Tom Coenye
Summary: D,L-malate enhances ciprofloxacin activity against P. aeruginosa, improving antibiotic therapy in infection environments. The choice of model system influences the outcome of the experiment, emphasizing the importance of using models that closely mimic the in vivo environment. This study confirms the potential of D,L-malate to enhance ciprofloxacin activity against P. aeruginosa-associated infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Public, Environmental & Occupational Health
Rodrigo C. Menezes, Isabella B. B. Ferreira, Luciana Sobral, Stefania L. Garcia, Hugo N. Pustilnik, Mariana Araujo-Pereira, Bruno B. Andrade
Summary: This study aimed to identify the clinical features associated with viral pathogens responsible for severe lower respiratory tract infections (LRTI) in children. The study found that different viral agents have distinct associations with clinical features in children.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2024)
Article
Public, Environmental & Occupational Health
Ambrina Khatoon, Syed F. Hussain, Syed M. Shahid, Santosh Kumar Sidhwani, Salman Ahmed Khan, Omer Ahmed Shaikh, Abdulqadir J. Nashwan
Summary: Despite the increasing incidence of Staphylococcus aureus infection and dissemination in Pakistan, research on the epidemiology of different Staphylococcus aureus clones has been limited. This study used multilocus sequence typing (MLST) to analyze the epidemiology of Staphylococcus aureus in the area, finding high diversity of locally circulating clones defined by their geographic epidemiology.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2024)
Review
Public, Environmental & Occupational Health
Amir Khorram-Manesh, Krzysztof Goniewicz, Frederick M. Burkle Jr
Summary: This article discusses the management approach for globalized diseases in a globalized world. Through literature review and analysis, key focuses including data-driven decision-making, robust technology infrastructure, global cooperation, and ongoing public health education are identified. The weaknesses of current pandemic management systems are revealed, and recommendations for strengthening future pandemic management are provided.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2024)
Article
Public, Environmental & Occupational Health
Mst S. Munira, Yuta Okada, Hiroshi Nishiura
Summary: This study estimates the life expectancy at birth in Japan at the end of 2022 using death datasets from Aichi and Fukui prefectures. The results suggest that the impact of the pandemic on life expectancy was relatively small by the end of 2022.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2024)