Influence of an anti-metastatic ruthenium(III) prodrug on extracellular protein-protein interactions: studies by bio-layer interferometry

In the first application of bio-layer interferometry in medicinal inorganic chemistry, Fe(III)-transferrin (Tf) binds strongly to Tf receptor 1 (TfR1), but an apo-Tf adduct of the anti-metastatic prodrug, NAMI-A does not bind specifically to TfR1. Binding of NAMI-A to albumin affects its interactions with other blood proteins, such as immunoglobulins.