Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 456, Issue 2, Pages 605-609Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2014.11.121
Keywords
Bone morphogenetic protein; Insulin; Cardiomyocyte; Apoptosis
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Funding
- Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang [2011CJHB002]
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Bone morphogenetic protein-4 (BMP4) mediates pathological cardiac hypertrophy. Insulin is well-known to promote cardiomyocyte survival in heart diseases. The aim of the present study is to evaluate the effects of insulin on BMP4-induced cardiomyocyte apoptosis. Cell viability and apoptosis were measured by using MU, live and dead staining, caspase-3 activity assays, and the protein expressions were measured by using western blot technique. Insulin did not elicit cardiomyocyte apoptosis, but antagonized BMP4-induced cardiomyocyte apoptosis. Insulin treatment rapidly activated Akt which was inhibited by Akt inhibitor in cardiomyocytes. Furthermore, Akt inhibitor canceled the anti-apoptotic effects of insulin against BMP4 in cardiomyocytes. In conclusion, insulin prevents BMP4-induced cardiomyocyte apoptosis and the underlying mechanisms include activation of Akt. The present work provides a novel mechanism of the protective effects of insulin in cardiovascular system. (C) 2014 Elsevier Inc. All rights reserved.
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