Review
Immunology
Meng Zhang, Na Li, Yanchao He, Tianyun Shi, Zhijun Jie
Summary: This article mainly reviews the development and regulation of various TRM cell signaling pathways in the respiratory tract, explores the protective roles of TRM cells in immune response against respiratory viruses, and discusses the complex roles of TRM cells against SARS-CoV-2 infection.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Marion Griessl, Angelique Renzaho, Kirsten Freitag, Christof K. Seckert, Matthias J. Reddehase, Niels A. W. Lemmermann
Summary: This study investigated the transcription of viral genes during latent infection of murine cytomegalovirus, revealing that the transcription of antigenic peptides driving memory inflation rarely coincides with the transcription of immune evasion proteins. The stochastic transcription hypothesis explains why immune evasion does not interfere with memory inflation in latently infected cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Xin Cheng, Gan Zhao, Aihua Dong, Zhonghuai He, Jiarong Wang, Brian Jiang, Bo Wang, Miaomiao Wang, Xuefen Huai, Shijie Zhang, Shuangshuang Feng, Hong Qin, Bin Wang
Summary: This study conducted a first-in-human trial to evaluate the safety and immunogenicity response of the BARS13 vaccine in healthy adults. The vaccine demonstrated good safety and tolerability, and there were no significant differences in adverse reaction severity or frequency between different dose groups. The immune response in repeat-dose recipients showed potential for further study and guiding the dose selection for future studies.
Article
Immunology
Marsha S. Russell, Sathya N. Thulasi Raman, Caroline Gravel, Wanyue Zhang, Annabelle Pfeifle, Wangxue Chen, Gary Van Domselaar, David Safronetz, Michael Johnston, Simon Sauve, Lisheng Wang, Michael Rosu-Myles, Jingxin Cao, Xuguang Li
Summary: A novel recombinant RSV vaccine candidate based on a deletion mutant vaccinia virus platform showed high safety and efficacy in experiments, suggesting its potential for developing new vaccines.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Augusto Varese, Joy Nakawesi, Ana Farias, Freja C. M. Kirsebom, Michelle Paulsen, Rinat Nuriev, Cecilia Johansson
Summary: Respiratory syncytial virus (RSV) infection presents challenges in eliciting protective memory immune responses. This study uses mouse models to investigate the impact of innate immune response determinants on T-RM cell generation and expansion during RSV infection. The results show that impairment of innate immune recognition and induction of type I interferons lead to reduced expansion and functionality of T-RM cells. Interestingly, treatment with interferon-alpha can restore T-RM cell expansion but not functionality. These findings provide insights into the role of innate immunity in regulating T-RM cell responses to RSV infection.
Article
Multidisciplinary Sciences
David L. Rose, Katie L. Reagin, Kimberly E. Oliva, S. Mark Tompkins, Kimberly D. Klonowski
Summary: NK cells play a crucial role in the development of anti-influenza CD8(+) T cell memory, and their removal results in an increase in influenza-specific memory CD8(+) T cells. Protection in NK-deficient animals during primary influenza infection is attributed to rapid reactivation of lung tissue-resident (T-RM) memory cells. Additionally, the development of T-RM is independent of global and NK cell-derived IFN-gamma.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Ju Jia, Hui Li, Zhisheng Huang, Jiapei Yu, Ying Zheng, Bin Cao
Summary: This study investigates the transitions and regulation mechanisms of CD8(+) T cells after influenza virus infection and reinfection. The findings show that CD8(+) Trm cells accumulate in the lung after infection, with a subset of these cells maintaining high expression of CD49a even 90 days after primary infection. The ratio of CD8(+) Trm cells decreases after reinfection, potentially indicating a transition into effector types. Additionally, various signaling pathways and cell interactions, including the Ccl5-Ccr5 ligand/receptor pair, play important roles in the function and interactions of CD8(+) Trm cells after infection and reinfection.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Nicholas N. Jarjour, Kelsey M. Wanhainen, Changwei Peng, Noah V. Gavil, Nicholas J. Maurice, Henrique Borges da Silva, Ryan J. Martinez, Talia S. Dalzell, Matthew A. Huggins, David Masopust, Sara E. Hamilton, Stephen C. Jameson
Summary: Interleukin-15 (IL-15) is a central regulator of memory CD8+ T cells and can stimulate their proliferation and expansion. IL-15 sensitivity is an important feature of memory CD8+ T cell populations, with therapeutic potential.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Felicia Scaggs Huang, David I. Bernstein, Karen S. Slobod, Allen Portner, Toru Takimoto, Charles J. Russell, Michael Meagher, Bart G. Jones, Robert E. Sealy, Christopher Coleclough, Kristen Branum, Michelle Dickey, Kristen Buschle, Monica McNeal, Mat Makowski, Aya Nakamura, Julia L. Hurwitz
Summary: The study demonstrated that SeVRSV vaccine was well-tolerated in healthy adults with only mild to moderate reactions and no severe adverse events. Transient vaccine genome detection was observed due to preexisting immunity towards hPIV-1 and RSV in adults. Minimal antibody responses to SeV and negligible responses to RSV F were observed.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2021)
Review
Biochemistry & Molecular Biology
Zifang Shang, Shuguang Tan, Dongli Ma
Summary: Respiratory syncytial virus (RSV) is a significant viral pathogen causing respiratory tract infections in infants, the elderly, and immunocompromised individuals globally. Over the past decade, there has been substantial progress in understanding the structure, function, and interactions of RSV components, as well as the host immune responses to infection. Researchers and pharmaceutical companies have shown widespread interest in searching for effective interventions for RSV infection, leading to the screening of promising monoclonal antibodies, inhibitors, and vaccine candidates.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Biology
Scott M. Anthony, Natalija Van Braeckel-Budimir, Steven J. Moioffer, Stephanie van de Wall, Qiang Shan, Rahul Vijay, Ramakrishna Sompallae, Stacey M. Hartwig, Isaac J. Jensen, Steven M. Varga, Noah S. Butler, Hai-Hui Xue, Vladimir P. Badovinac, John T. Harty
Summary: IAV infection generates Trm CD8(+)T cells in mice, while repeated antigen exposure produces 4M CD8(+)T cells with enhanced protective capacity. Enhanced protection by 4M cells is associated with increased expression of granzyme A/B and stable maintenance of CD69(+)CD103(+) 4M CD8(+)T cells, improving control of viral infections in mLN.
Article
Biotechnology & Applied Microbiology
Ki-Back Chu, Su-Hwa Lee, Min-Ju Kim, Ah-Ra Kim, Eun-Kyung Moon, Fu-Shi Quan
Summary: The study demonstrates that co-expressing PreF and Gt antigens in VLPs can enhance protective efficacy against RSV in mice, leading to reduced virus titers and inflammation. These findings suggest that a combinatorial approach may be beneficial for future RSV vaccine development.
Article
Virology
Jorge C. G. Blanco, Lori M. Cullen, Arash Kamali, Fatouomata Y. D. Sylla, Marina S. Boukhvalova, Trudy G. Morrison
Summary: This study compared immune responses after virus-like particle (VLP) immunization in elderly and young cotton rats previously infected with RSV. The results showed that the VLP vaccine was equally effective in activating protective responses in both elderly and young populations. Therefore, VLPs containing F and G proteins can be an effective vaccine for the elderly.
Review
Immunology
Rut Mora-Buch, Shannon K. Bromley
Summary: Resident memory CD8(+) T cells provide rapid local protection and control tumor growth, but dysregulation may contribute to autoimmune diseases. Intrinsic mechanisms and extrinsic stimuli regulate T-RM differentiation and response.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Oludare A. Odumade, Simon D. van Haren, Asimenia Angelidou
Summary: Respiratory viral infections have a significant impact on global disease and mortality. The coronavirus disease 2019 pandemic has brought about drastic changes in the seasonality patterns and mitigation efforts of respiratory syncytial virus, highlighting the urgent need for vaccination as a preventive tool.
CLINICAL INFECTIOUS DISEASES
(2022)
Review
Oncology
Alec J. Redwood, Ian M. Dick, Jenette Creaney, Bruce W. S. Robinson
Summary: The process of tumorigenesis results in genetic changes in tumor cells that have potential to serve as tumor-specific immune targets when expressed. Neoantigen vaccines, harnessing this immunogenicity, have shown efficacy in preclinical models and are now in clinical trials. The challenges in the personalized neoantigen vaccine field include identifying targetable antigens and addressing preexisting immunosuppression, particularly T-cell exhaustion, for the development of effective cancer vaccines.
Article
Medicine, Research & Experimental
Yue-Peng Zhou, Meng-Ji Mei, Xian-Zhang Wang, Sheng-Nan Huang, Lin Chen, Ming Zhang, Xin-Yan Li, Hai-Bin Qin, Xiao Dong, Shuang Cheng, Le Wen, Bo Yang, Xue-Fang An, Ao-Di He, Bing Zhang, Wen-Bo Zeng, Xiao-Jun Li, Youming Lu, Hong-Chuang Li, Haidong Li, Wei-Guo Zou, Alec J. Redwood, Simon Rayner, Han Cheng, Michael A. McVoy, Qiyi Tang, William J. Britt, Xin Zhou, Xuan Jiang, Min-Hua Luo
Summary: The study developed a congenital murine CMV infection mouse model that mimicked clinical manifestations of cCMV infection in infants, allowing for investigation of mechanisms of infection-induced brain damage and the effectiveness of antiviral treatment in mitigating neurological functions and brain damage.
Article
Gastroenterology & Hepatology
Janine Kemming, Swantje Gundlach, Marcus Panning, Daniela Huzly, Jiabin Huang, Marc Luetgehetmann, Sven Pischke, Julian Schulze Zur Wiesch, Florian Emmerich, Sian Llewellyn-Lacey, David A. Price, Yakup Tanriver, Klaus Warnatz, Tobias Boettler, Robert Thimme, Maike Hofmann, Nicole Fischer, Christoph Neumann-Haefelin
Summary: Chronic HEV infection is associated with exhaustion of HEV-specific CD8+ T cells, indicating that T-cell failure is driven by persistent antigen recognition in severely immunosuppressed hosts. Functional reinvigoration of virus-specific T cells is at least partially possible when the antigen is cleared. Viral escape in a minority of patients also contributes to the failure of HEV-specific CD8+ T cells.
JOURNAL OF HEPATOLOGY
(2022)
Article
Cell Biology
Phillip Pymm, Stefan Tenzer, Edmund Wee, Mirjana Weimershaus, Anne Burgevin, Simon Kollnberger, Jan Gerstoft, Tracy M. Josephs, Kristin Ladell, James E. McLaren, Victor Appay, David A. Price, Lars Fugger, John I. Bell, Hansjoerg Schild, Peter van Endert, Maria Harkiolaki, Astrid K. N. Iversen
Summary: This study discovers that the cytotoxic T lymphocyte (CTL) and natural killer (NK) cell responses to a specific epitope in the human immunodeficiency virus are associated with enhanced immune control. The length and mutations of the epitope influence the CTL and NK cell responses, leading to viral escape.
Article
Cell Biology
Emily Phung, Lauren A. Chang, Maryam Mukhamedova, Lijuan Yang, Deepika Nair, Scott A. Rush, Kaitlyn M. Morabito, Jason S. McLellan, Ursula J. Buchholz, John R. Mascola, Michelle C. Crank, Grace Chen, Barney S. Graham, Tracy J. Ruckwardt
Summary: RSV pre-F vaccination results in expansion and activation of RSV and HMPV F-specific B cells that contribute to long-term pneumovirus immunity.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Hematology
Ohad Benjamini, Rotem Gershon, Erez Bar-Haim, Yaniv Lustig, Hila Cohen, Ram Doolman, Meirav Kedmi, Elena Ribakovsky, Abraham Kneller, Tammy Hod, Noam Erez, Itzhak Levy, Galia Rahav, Abraham Avigdor
Summary: This study assessed the immune response in patients with CLL after receiving the fourth dose of the BNT162b2 mRNA COVID-19 vaccine. The results showed that the serologic response in CLL patients improved to a lesser extent than in healthy controls, but there was an improvement in T-cell response. During the three months after the fourth vaccine, 21% of patients developed COVID-19 infection, but all recovered without complications.
EUROPEAN JOURNAL OF HAEMATOLOGY
(2023)
Article
Cell Biology
Lauren A. Chang, Emily Phung, Michelle C. Crank, Kaitlyn M. Morabito, Tonya Villafana, Filip Dubovsky, Judith Falloon, Mark T. Esser, Bob C. Lin, Grace L. Chen, Barney S. Graham, Tracy J. Ruckwardt
Summary: This study evaluated the effect of the respiratory syncytial virus (RSV) fusion protein (F) conformation on B cell responses in a comparison of two vaccine clinical trials. The results showed that DS-Cav1 vaccine elicited antibodies that more potently neutralized both RSV A and B, and activated B cells preferentially binding the pre-fusion probe. This highlights the importance of using pre-F as an immunogen for eliciting highly potent neutralizing antibodies and memory B cells.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Immunology
Davide Proietto, Beatrice Dallan, Eleonora Gallerani, Valentina Albanese, Sian Llewellyn-Lacey, David A. Price, Victor Appay, Salvatore Pacifico, Antonella Caputo, Francesco Nicoli, Riccardo Gavioli
Summary: Age-related changes in the immune system affect the ability of elderly individuals to generate effective CD8(+) T cell responses against SARS-CoV-2. Elderly individuals show a deficit in priming naive precursors into effector CD8(+) T cells, resulting in reduced diversity and expression frequency of IFN-gamma in CD8(+) T cell responses. These findings have implications for developing strategies to protect the elderly against COVID-19.
Article
Biology
Freya R. R. Shepherd, Kate Davies, Kelly L. L. Miners, Sian Llewellyn-Lacey, Simon Kollnberger, James E. E. Redman, Melissa M. M. Grant, Kristin Ladell, David A. A. Price, James E. E. McLaren
Summary: Severe bacterial or viral infections can lead to an overactive immune response known as a cytokine storm. Superantigens are potent toxins that activate T cells non-specifically, leading to T cell dysfunction and immune evasion. This study found that TRBV12-3/12-4(+) T cells are highly responsive to streptococcal pyrogenic exotoxin C (SpeC) and toxic shock syndrome toxin-1 (TSST-1).
COMMUNICATIONS BIOLOGY
(2023)
Article
Immunology
Lisa Ciacchi, Martijn D. B. van de Garde, Kristin Ladell, Carine Farenc, Martien C. M. Poelen, Kelly L. Miners, Carmen Llerena, Hugh H. Reid, Jan Petersen, David A. Price, Jamie Rossjohn, Cecile A. C. M. van Els
Summary: A new research found that the immune responses against Streptococcus pneumoniae are mediated by CD4+ T cells. The study identified an immunodominant CD4+ T cell epitope from pneumolysin, which can be presented by different HLA allotypes and recognized by diverse TCRs. The immunogenicity of this epitope is determined by core residues in a conserved undecapeptide region, enabling cross-recognition of heterologous bacterial pathogens. These findings provide insights into the immune response against bacterial infections and can inform strategies to combat life-threatening infectious diseases.
Review
Immunology
Marcus Buggert, David A. Price, Laura K. Mackay, Michael R. Betts
Summary: Our current understanding of human memory CD8(+) T cells mainly comes from studies of the intravascular space, but new data challenges some established ideas and suggests the need for conceptual revision. This review provides a brief history of the field and summarizes the biology of circulating and tissue-resident memory CD8(+) T cells, which play a crucial role in immune surveillance. The authors also discuss how future human studies can improve our understanding of CD8(+) T cells and inform the development of better immunotherapies and vaccines.
Article
Multidisciplinary Sciences
Peter W. Krug, Lingshu Wang, Wei Shi, Wing-Pui Kong, Daniel L. Moss, Eun Sung Yang, Brian E. Fisher, Kaitlyn M. Morabito, John R. Mascola, Masaru Kanekiyo, Barney S. Graham, Tracy J. Ruckwardt
Summary: The study demonstrates that virus-like particle (VLP) vaccines can generate a protective neutralizing antibody against homologous and heterologous EV-D68 subclades. The B1 subclade VLP vaccine showed comparable neutralizing activity as an inactivated viral particle vaccine, while the B3 VLP vaccine exhibited improved cross-neutralization against heterologous viruses. The use of a carbomer-based adjuvant, Adjuplex, achieved a balanced CD4+ T helper response and generated robust neutralizing antibodies against both homologous and heterologous subclade viruses.
Article
Multidisciplinary Sciences
Edo Kon, Yinon Levy, Uri Elia, Hila Cohen, Inbal Hazan-Halevy, Moshe Aftalion, Assaf Ezra, Erez Bar-Haim, Gonna Somu Naidu, Yael Diesendruck, Shahar Rotem, Nitay Ad-El, Meir Goldsmith, Emanuelle Mamroud, Dan Peer, Ofer Cohen
Summary: This study developed an effective mRNA-LNP vaccine against a lethal bacterial pathogen by optimizing mRNA payload and antigen design. The vaccine elicited immune responses and provided rapid, full protection against the bacterial infection in mice after a single dose. These findings open avenues for urgently needed effective antibacterial vaccines.
Correction
Immunology
Marcus Buggert, David A. Price, Laura K. Mackay, Michael R. Betts
Article
Biochemistry & Molecular Biology
Noam Erez, Hagit Achdout, Yfat Yahalom-Ronen, Shimrit Adutler-Lieber, Liat Bar-On, Erez Bar-Haim, Boaz Politi, Einat B. Vitner, Hadas Tamir, Sharon Melamed, Nir Paran, Tomer Israely
Summary: Following viral infection, T-cells play a crucial role in the immune response. To evaluate the cellular response after vaccination or infection, a reliable activity assay is needed. This study describes two methods, peptide library and computational scanning combined with a cellular activity assay, to identify T cell epitopes of the SARS-CoV-2 surface glycoprotein. Four CD8 T cell epitopes were identified, including a novel epitope. This research could aid in antigen design for vaccination and establish cellular activity assays for emerging pathogens.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)