Review
Immunology
Ziming Hu, Xiuxiu Xu, Haiming Wei
Summary: NK cells are essential components of innate immunity, playing a key role in defending against tumors and viral infections. They express a diverse range of receptors and exhibit phenotypic and functional diversity influenced by various factors such as developmental origins, tumors, and viral infections. Understanding NK cells in the tumor microenvironment is crucial for the development of NK cell immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Christian Binder, Felix Sellberg, Filip Cvetkovski, Stefan Berg, Erik Berglund, David Berglund
Summary: The study showed that siplizumab increased NK cell activation by binding to Fc gamma RIIIA, suggesting potential application in inhibiting NK cell activation. However, the inhibitory effects of CD2 blockade on natural cytotoxicity and antibody-dependent cytotoxicity remain unclear.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biophysics
Hongmei Yang, Hao Jia, Qi Zhao, Kathy Qian Luo
Summary: Tumor immunotherapy, especially NK cell-based therapy, has gained attention due to its effectiveness with fewer side effects. This study establishes a 3D coculture model using fluorescence resonance energy transfer (FRET) and zebrafish, allowing real-time visualization of the interaction and killing process of NK cells against cancer cells at single-cell resolution. The findings demonstrate the potential of NK cell therapy and provide a valuable in vivo tool for its development and evaluation.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Cell Biology
Jonathan Kuhn, Yiyan Lin, Peter N. Devreotes
Summary: Dictyostelium discoideum has been a valuable tool for dissecting eukaryotic cell biology due to its short growth cycle and genetic tractability. Recent advances in imaging, synthetic biology, and computational analysis allow researchers to tune the activity of individual molecules in living cells and measure the effects on cellular motility and signaling. This enables a better understanding and control of cell migration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Fernanda Scopelliti, Valentina Dimartino, Caterina Cattani, Andrea Cavani
Summary: This study found that TRPA1 channels are highly expressed in the CD56(dim)CD16(+) subpopulation of NK cells, and their activation can increase cell-mediated cytotoxicity against tumors while also regulating the survival of NK cells to limit excessive cytotoxicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Josephine Moran, Liam Feltham, James Bagnall, Marie Goldrick, Elizabeth Lord, Catherine Nettleton, David G. Spiller, Ian Roberts, Pawel Paszek
Summary: The paper investigates the real-time interaction between the food-borne pathogen L. monocytogenes and host macrophages, revealing heterogeneous outcomes of infection and the impact of bacterial quantity on macrophage phagocytosis ability. It also highlights the role of population-level PrfA-mediated activity in regulating host-pathogen interactions.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Grace Lee, Sheela Karunanithi, Bruce Posner, Hanspeter Niederstrasser, Hong Cheng, Yuriy Federov, Shivaprasad Manjappa, Karam Musaitif, Huaiyu Wang, Zachary Jackson, David Wald
Summary: Our work led to the identification of a promising compound that potently increases NK cell-mediated killing of a variety of different cancer cells, but has no impact on the killing of normal cells. This compound demonstrates the utility of this screening assay.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2022)
Article
Multidisciplinary Sciences
Ashley Teufel, Wu Liu, Jeremy A. Draghi, Craig E. Cameron, Claus O. Wilke
Summary: Viruses experience selective pressure on the timing and order of events during infection. A method has been developed to examine the dynamic phenotypic landscape that viruses must face during replication. Parameters associated with translation and early stage viral replication processes are essential for capturing experimentally observed dynamics.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, Research & Experimental
D. O. Bazhenov, V. A. Mikhailova, K. L. Furaeva, L. P. Vyaz'mina, D. Sokolov, S. A. Sel'kov
Summary: This study examined the effects of placenta-secreted cytokines on trophoblast cells. Proinflammatory cytokines such as IL-6, IFN gamma, and IL-1 beta increased the expression of TGF beta R2 by trophoblast cells, while VEGF and PLGF increased the expression of CD45, CD29, and CD54 adhesion molecules. The antiinflammatory cytokine IL-4 increased LeptinR expression by trophoblast cells. PMA and TNF alpha enhanced the adhesion of NK cells to trophoblast cells during migration processes.
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
(2022)
Article
Biology
Thomas G. W. Graham, John Joseph Ferrie, Gina M. Dailey, Robert Tjian, Xavier Darzacq
Summary: Single-molecule imaging is a powerful tool for studying biochemical processes in live cells, but tracking single molecules and detecting their interactions simultaneously remains challenging. This study describes a novel property of rhodamine dyes, proximity-assisted photoactivation (PAPA), which allows the detection of protein-protein interactions in live cells using two different labeled protein complexes in proximity.
Review
Cell & Tissue Engineering
Hans Klingemann
Summary: The NK-92 cell line is a highly active blood natural killer (NK) cell that exhibits broader and greater cytotoxicity. It has significant implications in both research and clinical treatment, showing great potential in the treatment of cancer and infections.
Article
Chemistry, Multidisciplinary
Jeehun Park, Yerin Shin, Jung Min Kim, SoonHo Kweon, Ah Young Song, Yujin Baek, Jinho Kim, Duck Cho, Hun Sik Kim, Junsang Doh
Summary: NK cells are innate lymphoid cells that play a crucial role in immune surveillance and defense. Impaired NK cell functions could indicate a higher susceptibility to infection or tumor development. Developing a comprehensive test that measures both cytotoxicity and cytokine secretion simultaneously is important for accurately assessing NK cell activity.
Article
Immunology
Qi Li, Suna Cai, Mengjiao Li, Xiaojie Zhou, Gao Wu, Kang Kang, Jingli Yuan, Ruohan Wang, Huyan Ting, Wei Zhang
Summary: This study found that NK cells from lung cancer patients had significantly decreased colony formation and cytotoxicity, upregulated NKG2A expression, and downregulated CD226 expression, all of which were significantly correlated with lung cancer occurrence.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Gal Manella, Dan Aizik, Rona Aviram, Marina Golik, Gad Asher
Summary: The study introduces a high-throughput method, called Circa-SCOPE, for generating high-resolution Phase Transition Curves (PTCs) by continuous monitoring of single-cell oscillations. This method allows comprehensive analysis and screening of circadian clocks' resetting cues, showing potential for both basic and translational research.
NATURE COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Niklas Sandstroem, Ludwig Brandt, Patrick A. Sandoz, Chiara Zambarda, Karolin Guldevall, Malte Schulz-Ruhtenberg, Bernd Roesener, Robin A. Krueger, Bjorn Oenfelt
Summary: Miniaturization of cell culture substrates allows for controlled analysis of living cells in confined micro-scale environments. In this study, glass microwell arrays were produced using laser-induced deep etching (LIDE) and evaluated for various microscopy-based cell assays. The results show that these microwells support viable cell cultures and imaging with single cell resolution, with different designs having specific benefits for different applications.
Article
Biochemical Research Methods
K. Olofsson, V. Carannante, M. Ohlin, T. Frisk, K. Kushiro, M. Takai, A. Lundqvist, B. Onfelt, M. Wiklund
Article
Multidisciplinary Sciences
Per E. Olofsson, Ludwig Brandt, Klas E. G. Magnusson, Thomas Frisk, Joakim Jalden, Bjorn Onfelt
SCIENTIFIC REPORTS
(2019)
Article
Acoustics
Bjorn Hammarstrom, Nils R. Skov, Karl Olofsson, Henrik Bruus, Martin Wiklund
Summary: This study introduces a novel concept for acoustic trapping of particles and cells, enabling dynamic trapping patterns in a simple and inexpensive setup by using a modified piezoelectric transducer in direct contact with the liquid sample. The system allows for dynamic arraying of levitated trapping sites at low power with potential applications in cell-based sample preparation and cell culture.
JOURNAL OF THE ACOUSTICAL SOCIETY OF AMERICA
(2021)
Article
Chemistry, Analytical
Karl Olofsson, Valentina Carannante, Madoka Takai, Bjorn Onfelt, Martin Wiklund
Summary: This study introduces an acoustic trapping-based core-shell MCTSs culture method for quantifying shell thickness and coverage percentage. It can be used for internal migration analysis within MCTSs and to study therapy response or the interaction between tumor and stromal cells.
Article
Biochemistry & Molecular Biology
Quentin Verron, Elin Forslund, Ludwig Brandt, Mattias Leino, Thomas W. Frisk, Per E. Olofsson, Bjorn Onfelt
Summary: The spatial distribution of activating ligands influences the formation and outcome of NK cell synapses. A combination of LFA-1 and CD16 engagement is necessary for mature synapses and degranulation. The organization of ligands in the synapse also affects its characteristics.
Article
Medicine, General & Internal
Annelie Johansson, Athanasia E. Christakou, Adina Iftimi, Mikael Eriksson, Jose Tapia, Lambert Skoog, Christopher C. Benz, Kenny A. Rodriguez-Wallberg, Per Hall, Kamila Czene, Linda S. Lindstrom
Summary: The study found that the risk of benign breast diseases varies by age, hormonal factors, and family history of breast cancer. Factors such as nulliparity, oral contraceptive use, hormone replacement therapy, obesity, and family history were associated with different risks of various breast disease subtypes, highlighting the importance of understanding benign breast diseases for breast cancer prevention.
Article
Chemistry, Analytical
Ksenia Loskutova, Karl Olofsson, Bjoern Hammarstroem, Martin Wiklund, Anna J. Svagan, Dmitry Grishenkov
Summary: In this study, the compressibility of droplets stabilized with cellulose nanofibers was estimated using acoustophoresis, showing a constant value across different droplet diameters. The results revealed a negative acoustic contrast factor for the droplets, indicating their relocation to pressure antinodes during ultrasonic actuation. The compressibility of the droplets was found to be higher than that of water but lower than that of pure perfluoropentane.
Article
Chemistry, Analytical
Hanna Ohlin, Thomas Frisk, Mattias Astrand, Ulrich Vogt
Summary: This study presents a miniaturized gold electroplating process for fabricating gold zone plates used in X-ray imaging experiments. The miniaturization is achieved by on-chip reference plating areas, providing a reliable way to control the height of the structures. The fabricated gold zone plates were successfully used in X-ray imaging experiments.
Article
Chemistry, Analytical
Bjorn Hammarstrom, Thomas J. Lane, Hazal Batili, Raymond Sierra, Martin Wiklund, Jonas A. Sellberg
Summary: Researchers developed an acoustic 2D focusing method for real-time online characterization of protein microcrystals, which improves the reliability and efficiency of serial femtosecond crystallography.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Bjorn Hammarstrom, Thomas J. Lane, Hazal Batili, Raymond Sierra, Martin Wiklund, Jonas A. Sellberg
Summary: This study developed an acoustic 2D focusing method for real-time online characterization of protein microcrystals in capillaries. By achieving focusing and controlling concentration, the data collection rate was improved, which aids in the development of improved sample delivery systems.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Joseph Rufo, Feiyan Cai, James Friend, Martin Wiklund, Tony Jun Huang
Summary: This primer provides an overview of the basic principles and various implementations of acoustofluidic technologies in biomedical applications. It aims to demystify the field and guide users in choosing the most suitable acoustofluidic technology for their research needs.
NATURE REVIEWS METHODS PRIMERS
(2022)
Article
Biochemical Research Methods
Karl Olofsson, Bjorn Hammarstrom, Martin Wiklund
Article
Hematology
Dhifaf Sarha, Ludwig Brandt, Martin Felices, Karolin Guldevall, Todd Lenvik, Peter Hinderlie, Julie Curtsinger, Erica Warlick, Stephen R. Spellma, Bruce R. Blazar, Daniel J. Weisdorf, Sarah Cooley, Daniel A. Vallera, Bjorn Onfelt, Jeffrey S. Miller