Article
Medicine, General & Internal
Yoshiaki Usui, Yukari Taniyama, Mikiko Endo, Yuriko N. Koyanagi, Yumiko Kasugai, Isao Oze, Hidemi Ito, Issei Imoto, Tsutomu Tanaka, Masahiro Tajika, Yasumasa Niwa, Yusuke Iwasaki, Tomomi Aoi, Nozomi Hakozaki, Sadaaki Takata, Kunihiko Suzuki, Chikashi Terao, Masanori Hatakeyama, Makoto Hirata, Kokichi Sugano, Teruhiko Yoshida, Yoichiro Kamatani, Hidewaki Nakagawa, Koichi Matsuda, Yoshinori Murakami, Amanda B. Spurdle, Keitaro Matsuo, Yukihide Momozawa
Summary: This study reveals that certain genetic variants associated with Helicobacter pylori infection can increase the risk of gastric cancer. Individuals carrying these genetic variants and infected with H. pylori have an even higher risk of developing gastric cancer.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Silvia Salvatori, Irene Marafini, Federica Laudisi, Giovanni Monteleone, Carmine Stolfi
Summary: H. pylori infection contributes to the onset and development of gastric cancer through the mechanisms of chronic inflammation and DNA damage to gastric epithelial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
So Dam Lee, Haengdueng Jeong, Bo Ram Hwang, Byeong Min Yu, Yejin Cho, Ki Teak Nam, Hyunki Kim, Yong Chan Lee
Summary: Strains of Helicobacter pylori positive for CagA may contribute to the development of gastric cancer through the induction of CK2 activation and CK28 degradation. The downregulation of CK28 and subsequent activation of AKT and Snail signaling pathways may play a crucial role in the epithelial-to-mesenchymal transition (EMT) in gastric cancer. CK2 could be a potential molecular target for the treatment of H. pylori-infected gastric cancer.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Review
Oncology
Michelle C. C. Lim, Phatcharida Jantaree, Michael Naumann
Summary: Helicobacter pylori is a pathogenic bacterium that resides in the stomach lining and causes inflammation called type B gastritis. The chronic inflammation induced by H. pylori and other factors may lead to the development of stomach neoplasms and adenocarcinoma. Dysregulation of cellular processes in the stomach lining and microenvironment is a characteristic of H. pylori infection. In this review, we discuss the contradictory role of H. pylori in promoting or suppressing apoptosis in gastric epithelial cells and highlight the key processes in the microenvironment that contribute to apoptosis and gastric carcinogenesis.
Article
Medicine, General & Internal
Maidina Abuduwaili, Hidehiko Takigawa, Ryo Yuge, Hajime Teshima, Takahiro Kotachi, Yuji Urabe, Masanori Ito, Kazuhiro Sentani, Naohide Oue, Shiro Oka, Yasuhiko Kitadai, Shinji Tanaka
Summary: This study aimed to investigate the relationship between NHPH infection and gastritis and gastric cancer development. The results showed no significant difference in NHPH infection rates based on different Hp infection statuses, and the NHPH infection rates in gastric cancer patients were similar to those in patients with gastritis-related diseases reported in previous studies. A comparison between NHPH-positive and negative patients showed no significant differences in atrophic gastritis status, serum gastritis markers, or clinicopathological characteristics of gastric cancer.
AMERICAN JOURNAL OF THE MEDICAL SCIENCES
(2023)
Article
Oncology
Mahjerin Nasrin Reza, Shahin Mahmud, Nadim Ferdous, Ishtiaque Ahammad, Mohammad Uzzal Hossain, Md. Al Amin, A. K. M. Mohiuddin
Summary: In this study, two siRNA molecules were designed to silence the CagA and VacA genes of H. pylori, which are significantly involved in gastric cancer development. These designed siRNAs should effectively silence the CagA and VacA genes during siRNA mediated treatment in gastric cancer.
Editorial Material
Microbiology
Agustina Taglialegna
Summary: In this study, Sharafutdinov et al. report the identification of a single nucleotide polymorphism in the gene encoding the HtrA protease of Helicobacter pylori that is linked to gastric cancer.
NATURE REVIEWS MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Tomohiko Yasuda, Hyun Seok Lee, Su Youn Nam, Hiroto Katoh, Yuko Ishibashi, Somay Yamagata Murayama, Hidenori Matsui, Hiroki Masuda, Emiko Rimbara, Nobuyuki Sakurazawa, Hideyuki Suzuki, Hiroshi Yoshida, Yasuyuki Seto, Shumpei Ishikawa, Seong Woo Jeon, Masahiko Nakamura, Sachiyo Nomura
Summary: Genetic analysis and culturing techniques for gastric non-Helicobacter pylori Helicobacter (NHPH) are advancing, with NHPH reported to accompany certain gastric conditions. A study on Korean gastric cancer patients found a low percentage positive for NHPH, suggesting it may play a role in gastric cancer development, albeit with lower pathogenicity compared to H. pylori.
SCIENTIFIC REPORTS
(2022)
Article
Gastroenterology & Hepatology
Jun-Hyung Cho, So-Young Jin, Suyeon Park
Summary: A 4-point discriminative model was developed based on the ABC test results to identify patients with a normal serological test who are still at risk of developing gastric cancer. The model showed similar accuracy to the ABC method in predicting gastric cancer risk, with an AUC of 0.894 in the validation cohort.
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Immunology
Rui M. Ferreira, Joana Figueiredo, Ines Pinto-Ribeiro, Irene Gullo, Dionyssios N. Sgouras, Laura Carreto, Patricia Castro, Manuel A. Santos, Fatima Carneiro, Raquel Seruca, Ceu Figueiredo
Summary: Helicobacter pylori infection induces overexpression of laminin gamma 2 in gastric cancer cells, promoting cell invasion and resistance to apoptosis through modulation of Src, JNK, and AKT activity. Laminin gamma 2 and its downstream effectors could be potential therapeutic targets, and H. pylori eradication may delay the onset and progression of gastric cancer.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Rejina Shrestha, Naoko Murata-Kamiya, Satoshi Imai, Masami Yamamoto, Tetsuya Tsukamoto, Sachiyo Nomura, Masanori Hatakeyama
Summary: The initial step in bacterial infection is the adherence of the bacterium to the target cell surface. This study found that the mouse stomach has a much lower expression level of Ceacam1 compared to the human stomach, which leads to the inability of the bacteria to deliver the CagA protein due to the lack of interaction between HopQ and Ceacam1 in mouse gastric epithelial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Aina Ichihara, Hinako Ojima, Kazuyoshi Gotoh, Osamu Matsushita, Susumu Take, Hiroyuki Okada, Akari Watanabe, Kenji Yokota
Summary: This study aimed to explore the association between different antibody reactivities and bacterial genome organization. High-reactive strains showed high gene homology, while inversion around the vacA gene was found in the genome of poorly responsive antigen strains.
Article
Biochemical Research Methods
Lusheng Song, Minkyo Song, Charles S. Rabkin, Stacy Williams, Yunro Chung, Jennifer Van Duine, Linda M. Liao, Kailash Karthikeyan, Weimin Gao, Jin G. Park, Yanyang Tang, Jolanta Lissowska, Ji Qiu, Joshua LaBaer, M. Constanza Camargo
Summary: This study evaluated humoral responses to a nearly complete H. pylori immunoproteome among GC cases and controls, finding higher prevalence of certain antibodies in controls and lower seroprevalence in GC cases, suggesting immune protection and potential changes in specific proteins.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Clara Lettl, Franziska Schindele, Ahmad Reza Mehdipour, Thomas Steiner, Diana Ring, Ruth Brack-Werner, Baerbel Stecher, Wolfgang Eisenreich, Ursula Bilitewski, Gerhard Hummer, Matthias Witschel, Wolfgang Fischer, Rainer Haas
Summary: Respiratory complex I plays an important role in cellular respiration of both eukaryotic cells and bacteria. The inhibition of complex I by mitochondrial complex I inhibitors can selectively kill Helicobacter pylori, a Gram-negative bacterial pathogen, while leaving other bacteria unaffected. The unique composition of the quinone-binding pocket in H. pylori complex I is responsible for this hypersensitivity, suggesting the potential of developing complex I inhibitors as narrow-spectrum antimicrobial agents against H. pylori.
CELL CHEMICAL BIOLOGY
(2023)
Review
Oncology
Naoko Murata-Kamiya, Masanori Hatakeyama
Summary: Infection with cagA-positive Helicobacter pylori strains can lead to the development of gastric cancer and is associated with genomic changes in host cells. Inside the host cells, the CagA protein interacts with multiple host cell proteins, disrupting normal cell signaling. H. pylori infection also causes DNA damage and affects DNA repair mechanisms. Once driver gene mutations occur, H. pylori CagA is no longer necessary for gastric carcinogenesis.
Article
Immunology
Ian D. Plumb, K. Danielle Lecy, Rosalyn Singleton, Michael C. Engel, Matthew Hirschfeld, James W. Keck, Joseph Klejka, Karen M. Rudolph, Thomas W. Hennessy, Michael G. Bruce
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2018)
Article
Multidisciplinary Sciences
Alyssa Bilinski, Ermyas Birru, Matthew Peckarsky, Michael Herce, Noel Kalanga, Christian Neumann, Gay Bronson, Stephen Po-Chedley, Chembe Kachimanga, Ryan McBain, James Keck
Article
Endocrinology & Metabolism
Karen L. Roper, Alisha R. Thomas, Laura Hieronymus, Audrey Brock, James Keck
Article
Primary Health Care
James W. Keck, Alisha R. Thomas, Laura Hieronymus, Karen L. Roper
JOURNAL OF THE AMERICAN BOARD OF FAMILY MEDICINE
(2019)
Article
Public, Environmental & Occupational Health
James W. Keck, Karen L. Roper, Laura B. Hieronymus, Alisha R. Thomas, Zhengyuan Huang, Philip M. Westgate, John L. Fowlkes, Roberto Cardarelli
AMERICAN JOURNAL OF PREVENTIVE MEDICINE
(2020)
Letter
Geriatrics & Gerontology
Mark J. Huffmyer, James W. Keck, Nancy Grant Harrington, Patricia R. Freeman, Matthew Westling, Kaylee M. Lukacena, Daniela C. Moga
JOURNAL OF THE AMERICAN GERIATRICS SOCIETY
(2021)
Article
Multidisciplinary Sciences
James W. Keck, Matthew Bush, Robert Razick, Setareh Mohammadie, Joshua Musalia, Joel Hamm
Summary: Smell testing was superior to symptom screening for identifying SARS-CoV-2 infection in our study.
Article
Health Care Sciences & Services
Megan Leigh Hutchcraft, Shulin Zhang, Nan Lin, Ginny Lee Gottschalk, James W. Keck, Elizabeth A. Belcher, Catherine Sears, Chi Wang, Kun Liu, Lauren E. Dietz, Justine C. Pickarski, Sainan Wei, Roberto Cardarelli, Robert S. DiPaola, Jill M. Kolesar
Summary: This study aimed to assess the clinical utility of germline medical exome sequencing in patients recruited from a family medicine clinic and compare the mutation frequency of hereditary predisposition genes to established general population frequencies. Results showed that hereditary disease predisposition gene mutations were identified in 6.3% of the patients and 69.2% of those underwent subsequent clinical interventions. Additionally, pharmacogenomic variants were identified in 76.1% of the patients, with 4.9% being prescribed medication with pharmacogenomic implications.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Editorial Material
Public, Environmental & Occupational Health
James W. Keck, Scott M. Berry
AMERICAN JOURNAL OF PUBLIC HEALTH
(2023)
Article
Public, Environmental & Occupational Health
Joel Hamm, Meredith S. Duncan, Nicole M. Robertson, James W. Keck, Katherine Crabtree
Summary: COVID-19 has heavily impacted the refugee population in the United States, and they have a higher rate of COVID-19 diagnosis in hospitals but lower odds of hospitalization and shorter length of stay.
JOURNAL OF IMMIGRANT AND MINORITY HEALTH
(2023)
Article
Environmental Sciences
Soroosh Torabi, Atena Amirsoleimani, Mohammad Dehghan Banadaki, William Dalton Strike, Alexus Rockward, Ann Noble, Matthew Liversedge, James W. Keck, Scott M. Berry
Summary: Wastewater-based Epidemiology (WBE) has been effective in surveillance of SARS-CoV-2 worldwide. However, the instability of SARS-CoV-2 RNA in wastewater limits the utility of WBE programs, especially in remote areas. This study found that immediate RNA extraction and ambient storage can increase the RNA half-life, and a lightweight and portable extraction technology called exclusion-based sample preparation (ESP) can be used in remote settings.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Public, Environmental & Occupational Health
James W. Keck, Jess Lindner, Matthew Liversedge, Blazan Mijatovic, Cullen Olsson, William Strike, Anni Noble, Reuben Adatorwovor, Parker Lacy, Ted Smith, Scott M. Berry
Summary: This study evaluated the performance of wastewater surveillance for SARS-CoV-2 at long-term care facilities (LTCFs) in Kentucky. The results showed that the surveillance system had variable performance, but stakeholders found it feasible and expressed optimism about its potential.
JMIR PUBLIC HEALTH AND SURVEILLANCE
(2023)
Article
Engineering, Environmental
Mohammad Dehghan Banadaki, Soroosh Torabi, William D. Strike, Ann Noble, James W. Keck, Scott M. Berry
Summary: Wastewater-based epidemiology (WBE) has enabled the description of SARS-CoV-2 infections in populations, but its implementation is limited due to the need for expert staff, expensive equipment, and prolonged processing times. To overcome these limitations, an automated workflow based on a simplified method was developed, which is faster and cheaper than conventional WBE methods.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Pharmacology & Pharmacy
Kaylee M. Lukacena, James W. Keck, Patricia R. Freeman, Nancy Grant Harrington, Mark J. Huffmyer, Daniela C. Moga
Summary: The study aimed to assess patients' beliefs and attitudes towards deprescribing and identify facilitators and barriers to deprescribing. The findings showed that adults taking multiple medications expressed a high willingness to accept deprescribing if their doctor gave permission. Targeted strategies that consider patient characteristics, such as age and education level, may be necessary for successful communication between patients, primary care clinicians, and pharmacists.
THERAPEUTIC ADVANCES IN DRUG SAFETY
(2022)
Article
Environmental Sciences
William Strike, Atena Amirsoleimani, Abisola Olaleye, Ann Noble, Kevin Lewis, Lee Faulkner, Spencer Backus, Sierra Lindeman, Katrina Eterovich, Melicity Fraley, Mohammad Dehghan Banadaki, Soroosh Torabi, Alexus Rockward, Eli Zeitlow, Matthew Liversedge, James Keck, Scott Berry
Summary: Wastewater surveillance has become an important tool for describing SARS-CoV-2 prevalence during the COVID-19 pandemic. However, traditional wastewater analysis methods are limited by specialized equipment, expensive consumables, and complex workflows. A new method called exclusion-based sample preparation (ESP) has been developed, which simplifies the workflow and reduces time and consumable usage. A pilot study in university residence halls showed that ESP method is effective and useful for wastewater analysis.