Journal
BIOENGINEERED
Volume 5, Issue 3, Pages -Publisher
LANDES BIOSCIENCE
DOI: 10.4161/bioe.27607
Keywords
upstream open reading; frames; uORF; translation initiation; ribosome scanning; cellular stress; posttranscriptional regulation
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Funding
- NIA NIH HHS [R21 AG040360, 5R21AG040360-02] Funding Source: Medline
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We have employed upstream open reading frames (uORFs) to systematically tune the translation levels of recombinant proteins. We present the design principles that guided the development of this technology and provide information that may help others in implementing synthetic uORFs for their own applications. We also report on recent applications to our own research projects, including the coupling of uORF and translation initiation site (TIS) engineering with small molecule-inducible post-translational control. Finally, we discuss opportunities to investigate and potentially engineer gene-specific translational responses to cellular stress.
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