Article
Infectious Diseases
Zhaoying Liu, Qiuhua Xu, Pujun Liang, Zeren Peng, Huochun Yao, Han Zheng, Zongfu Wu
Summary: Streptococcus suis, a severe zoonotic and foodborne pathogen, has distinct population structure and pathogenicity based on various serotypes. This study focused on serotype 8 (SS8) and found that SS8 population exhibited geographical clustering and included strains with global outbreak potential. Several SS8 strains showed virulence in mice and zebrafish, but no specific virulence indicators were identified. All SS8 isolates were classified as multidrug-resistant, with a high prevalence of resistance genes for macrolides, lincosamides, and tetracyclines. These findings highlight the importance of SS8 surveillance.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Genetics & Heredity
Nattinee Kittiwan, Jessica K. Calland, Evangelos Mourkas, Matthew D. Hitchings, Susan Murray, Pakpoom Tadee, Phacharaporn Tadee, Kwanjit Duangsonk, Guillaume Meric, Samuel K. Sheppard, Prapas Patchanee, Ben Pascoe
Summary: This study samples and sequences isolates from healthy pigs in Northern Thailand, revealing extensive genetic diversity and antimicrobial resistance in Streptococcus suis. The presence of antimicrobial resistance genes on integrative and conjugative elements suggests a mobile gene pool that can be accessed by invasive disease isolates.
MICROBIAL GENOMICS
(2022)
Article
Microbiology
Shujie Wang, Gang Wang, Yan-Dong Tang, Siqi Li, Lei Qin, Menghang Wang, Yong-Bo Yang, Marcelo Gottschalk, Xuehui Cai
Summary: This study reveals the mechanisms underlying splenic lesions caused by Streptococcus suis infection, including apoptosis and activation of inflammatory responses. The findings provide new insights into the pathogenesis of S. suis.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Roel M. van Harten, Johanna L. M. Tjeerdsma-van Bokhoven, Astrid de Greeff, Melanie D. Balhuizen, Albert van Dijk, Edwin J. A. Veldhuizen, Henk P. Haagsman, Maaike R. Scheenstra
Summary: The study evaluates the prophylactic capacity of D-CATH-2 and its derivatives in mammalian cells, and finds that they possess strong antibacterial capacity and immunomodulatory function. Administering D-C(1-21) provides partial protection against S. suis infection and reduces disease severity and mortality.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Microbiology
Anusak Kerdsin, Rujirat Hatrongjit, Thidathip Wongsurawat, Piroon Jenjaroenpun, Peechanika Chopjitt, Parichart Boueroy, Nahuel Fittipaldi, Han Zheng, Marcelo Gottschalk
Summary: Streptococcus suis is a zoonotic pathogen that can cause invasive infections in both humans and pigs, with different serotypes leading to distinct clinical manifestations and infection characteristics. Some strains exhibit antimicrobial resistance while lacking specific virulence-associated genes.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biophysics
Kanjana Kunpatee, Kannika Khantasup, Kittinan Komolpis, Abdulhadee Yakoh, Suphachai Nuanualsuwan, Mohini M. Sain, Sudkate Chaiyo
Summary: This study developed an electrochemical lateral flow immunoassay (eLFIA) strip with high reproducibility for rapid and accurate detection of Streptococcus suis serotype 2. The strip integrated ratiometric electrochemical detection and LFIA, resulting in improved reproducibility compared to a single electrode. The biosensor showed good linearity and a low limit of detection, and was successfully applied in human serum samples.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Immunology
Mei-Fang Tan, Jia Tan, Fan-Fan Zhang, Hai-Qin Li, Hua-Yuan Ji, Shao-Pei Fang, Cheng-Cheng Wu, Yu-Ling Rao, Yan-Bin Zeng, Qun Yang
Summary: This study investigated the pleiotropic effects of exogenous glycogen on Streptococcus suis serotype 2 (SS2) through transcriptome sequencing. The results showed that glycogen induction significantly influenced the metabolic pathways and virulence-associated factors of SS2. Extracellular glycogen utilization enhanced the hemolytic activity, adhesion and invasion ability, and pathogenicity of SS2. These findings are important for understanding the adaptation and pathogenicity mechanisms of SS2.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Lu Wang, Jing Sun, Jiyu Zhao, Jieyu Bai, Yueling Zhang, Yao Zhu, Wanjiang Zhang, Chunlai Wang, Paul R. Langford, Siguo Liu, Gang Li
Summary: In this study, a high-fidelity detection and serotyping platform for Streptococcus suis serotype 2 was developed based on recombinase polymerase amplification (RPA) and a CRISPR-Cas12a system. The platform showed accurate and rapid detection with a detection limit of 10 CFU and the ability to differentiate serotypes. It is a suitable method for point-of-care detection.
Article
Microbiology
Chaoxiong Yue, Chenlu Hu, Peng Xiang, Siming Zhang, Hongde Xiao, Wei Zhou, Hui Jin, Deshi Shi, Jinquan Li, Lang Xu, Yushan Chen, Yan Zeng
Summary: Streptococcus suis (S. suis) is a significant swine and human pathogen that can cause severe meningitis with high mortality. This study demonstrates that autophagy serves as a cellular defense mechanism in microglial cells following S. suis infection, aiding in limiting bacterial invasion and microglial inflammation.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Yoonhang Lee, Nameun Kim, HeyongJin Roh, Diem Tho Ho, Jiyeon Park, Ju Yeop Lee, Yoon-Jae Kim, Hyo-Young Kang, Jungmin Lee, Jun-Young Song, Ahran Kim, Myoung Sug Kim, Miyoung Cho, Hye Sung Choi, Chan-Il Park, Do-Hyung Kim
Summary: This study investigates the serotype distribution, antimicrobial susceptibility, and presence of antimicrobial resistance genes (ARGs) in S. parauberis isolates in South Korea. It reveals a correlation between serotype and antimicrobial resistance, with certain ARGs being distributed in a serotype-dependent manner. Recent isolates tend to show increased resistance to certain antibiotics compared to older isolates, possibly due to shifts in antibiotic usage in aquaculture.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Rujirat Hatrongjit, Nahuel Fittipaldi, Piroon Jenjaroenpun, Thidathip Wongsurawat, Suwattana Visetnan, Han Zheng, Marcelo Gottschalk, Anusak Kerdsin
Summary: Streptococcus suis is a zoonotic pathogen that causes infections in humans and pigs. Two serotype 1 strains from a human patient and an asymptomatic pig were analyzed, revealing differences in pathotype, virulence-associated gene profile, minimum core genome typing, and antimicrobial resistance gene content. Both strains were susceptible to certain antibiotics but resistant to others, with specific genes responsible for these resistances. Variations in virulence-associated genes were also observed, indicating differences between the two strains. Phylogenetic analysis showed genetic similarities between human and porcine strains from specific regions.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Ching-Fen Wu, Siou-Hui Chen, Chi-Chung Chou, Chao-Min Wang, Szu-Wei Huang, Hung-Chih Kuo
Summary: This study comprehensively characterized S. suis isolates from diseased pigs in Taiwan, revealing the prevalence of serotypes 3, 7, and 8, and the presence of novel sequence types and clonal complexes. The isolates showed high susceptibility to multiple antimicrobial agents. The distribution of different serotypes varied in different tissue samples.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Servane Payen, David Roy, Anais Boa, Masatoshi Okura, Jean-Philippe Auger, Mariela Segura, Marcelo Gottschalk
Summary: The study evaluated the role of Lgt and Lsp enzymes in the pathogenesis of Streptococcus suis serotype 2 infections. Lack of these enzymes led to a significant reduction in S. suis' ability to activate phagocytic cells and induce pro-inflammatory mediators.
Article
Immunology
Quan Li, Xia Fei, Yuhang Zhang, Genglin Guo, Huoying Shi, Wei Zhang
Summary: The study identified a putative virulence-associated factor MutT unique to S. suis serotype 2 virulent strains, essential for the pathogenicity of SS2. MutT knockout mutant significantly decreased bacterial virulence and increased susceptibility to oxidative stress, while enhancing adhesion and invasion to host cells. Deletion of mutT also led to increased microbial clearance in infected host tissues, indicating the crucial biological role of MutT in bacterial pathogenesis during infection.
Article
Veterinary Sciences
Servane Payen, Jesus Aranda Rrodriguez, Mariela Segura, Marcelo Gottschalk
Summary: Streptococcus suis serotype 2 is a significant pathogen in swine, causing economic losses to the industry. The role of the Lmb protein in its pathogenesis was examined and found to have no effect on laminin-binding activity or bacterial adhesion and invasion. However, it was shown to activate cytokines in vitro and play a critical role in zinc acquisition from the host environment.
VETERINARY RESEARCH
(2023)
