Article
Biochemistry & Molecular Biology
Vetaka Tharasirivat, Saowarath Jantaro
Summary: The overexpression of the RuBisCO gene can increase cyanobacterial biomass and improve biocompound production. We successfully constructed two strains of Synechocystis sp. PCC 6803 that overexpressed the rbc gene, resulting in faster growth compared to the wild type strain. The increased biomass of these strains led to higher production of polyhydroxybutyrate (PHB) under nutrient-deprived conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Rajendran Velmurugan, Aran Incharoensakdi
Summary: Manipulating the expression of specific genes in cyanobacteria can enhance the production of EPS, leading to increased tolerance to high light intensity and UV irradiance.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Plant Sciences
Martin Lehmann, Evgenia Vamvaka, Alejandro Torrado, Peter Jahns, Marcel Dann, Lea Rosenhammer, Amel Aziba, Dario Leister, Thilo Ruehle
Summary: The study genetically engineered the cyanobacterium Synechocystis to accumulate lutein by introducing Arabidopsis genes, resulting in a low but distinct synthesis of lutein. This demonstrates the potential of using a cyanobacterial host system to study the alpha-branch of the plastidic MEP pathway and other functions related to lutein.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Microbiology
Jin Ding, Nils Schuergers, Heike Baehre, Annegret Wilde
Summary: This study discovered functional CARF-domain effector and ring nuclease proteins in the cyanobacterium Synechocystis sp. PCC 6803. The Csx1 homolog, SyCsx1, is a strict cytosine-specific RNase that depends on cyclic tetraadenylate (cA4). Another CARF-domain protein similar to Csm6 effectors, SyCsm6, does not have RNase activity but can degrade cOAs and attenuate SyCsx1 RNase activity. This research suggests that the CRISPR systems in Synechocystis have a multi-layered cA4-mediated defense mechanism.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Kateryna Kukil, Elias Englund, Nick Crang, Elton P. Hudson, Pia Lindberg
Summary: In this study, phenylalanine resistant mutant strains of the unicellular cyanobacterium Synechocystis sp. PCC 6803 were generated by laboratory evolution. The mutant strains were able to secrete phenylalanine and showed potential for production of trans-cinnamic acid and para-coumaric acid. The PRM8 strain demonstrated the highest specific production of these compounds.
METABOLIC ENGINEERING
(2023)
Article
Multidisciplinary Sciences
Raquel Garcia-Canas, Joaquin Giner-Lamia, Francisco J. Florencio, Luis Lopez-Maury
Summary: The study reveals the involvement of proteins PetR and PetP in regulating the soluble electron carrier in cells. Transcriptomic analysis shows that the PetRP system specifically regulates four genes (petE, petJ, slr0601, and slr0602), with their presence potentially representing an early adaptation to decreased iron bioavailability following the GOE.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemical Research Methods
Ian Sofian Yunus, Zhixuan Wang, Pachara Sattayawat, Jonathan Muller, Fessehaye W. Zemichael, Klaus Hellgardt, Patrik R. Jones
Summary: The study focused on improving the productivity of 1-octanol by enhancing enzyme efficiency and optimizing environmental conditions. By adjusting the inducer concentration and light intensity, the researchers were able to increase the 1-octanol productivity significantly, reaching a carbon partitioning rate of 35% and a titer of 526 mg/L within 12 days.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Eunice A. Ferreira, Catarina C. Pacheco, Joao S. Rodrigues, Filipe Pinto, Pedro Lamosa, David Fuente, Javier Urchueguia, Paula Tamagnini
Summary: This study successfully produced glycine betaine using the cyanobacterium Synechocystis sp. PCC 6803 as a chassis, through the construction of synthetic devices in mutant strains.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Sang-Hyeok Cho, Yujin Jeong, Seong-Joo Hong, Hookeun Lee, Hyung-Kyoon Choi, Dong-Myung Kim, Choul-Gyun Lee, Suhyung Cho, Byung-Kwan Cho
Summary: This study elucidated the inhibitory mechanisms of photosynthesis in cyanobacteria under high-light and low-temperature stress conditions, highlighting the differences in response mechanisms between the two stress conditions. Through multiomics data analysis and construction of genome-wide transcription unit architecture, factors affecting photosynthesis at transcription, posttranscription, and translation levels were identified, providing pivotal information for future cyanobacterial cell factories development.
Article
Biochemical Research Methods
Maurice Mager, Hugo Pineda Hernandez, Fabian Brandenburg, Luis Lopez-Maury, Alistair J. McCormick, Dennis J. Nuernberg, Tim Orthwein, David A. Russo, Angelo Joshua Victoria, Xiaoran Wang, Julie A. Z. Zedler, Filipe Branco dos Santos, Nicolas M. Schmelling
Summary: In recent years, many new synthetic biology tools have been developed for use in cyanobacteria. However, the reported characterizations of these tools often cannot be reproduced, which greatly limits comparability of results and applicability. This study assessed the reproducibility of a standard microbiological experiment in Synechocystis sp. PCC 6803 and found significant differences in spectrophotometer measurements and growth rates between laboratories. These findings highlight the need for additional reporting requirements of growth conditions for phototrophic organisms beyond light intensity and CO2 supply.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Zhang-He Zhen, Song Qin, Qing-Min Ren, Yu Wang, Yu-Ying Ma, Yin-Chu Wang
Summary: The study shows that changes in copper and iron concentrations influence the growth and photosynthesis of Synechocystis sp. PCC 6803, with some proteins involved in metal uptake and photosynthesis showing a Cu-Fe proteomic association. This proteomic association affects the photosynthetic electron activity and growth of the cyanobacteria.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Parveen Akhtar, Avratanu Biswas, Fanny Balog-Vig, Ildiko Domonkos, Laszlo Kovacs, Petar H. Lambrev
Summary: Cyanobacterial mutants with monomeric PSI show changes in the composition and abundance of phycobilisomes and in the excitation energy transfer to PSII and PSI. The oligomeric state of PSI impacts the excitation energy flow in Synechocystis.
Article
Plant Sciences
Nannaphat Sukkasam, Jidapa Leksingto, Aran Incharoensakdi, Tanakarn Monshupanee
Summary: This study examined the effects of various compounds on glycogen production in cyanobacteria and found that methyl viologen can stimulate glycogen accumulation, allowing for a one-stage cultivation method. Methyl viologen treatment reduced protein levels but increased transcript levels of glycogen synthetic genes and levels of metabolite substrates for glycogen synthesis. Metabolomic analysis suggested that proteins degraded to amino acids, which could serve as a carbon source for glycogen synthesis.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Engineering, Environmental
Javier Garcia-Calleja, Thibaut Cossart, Zoyne Pedrero, Joao P. Santos, Laurent Ouerdane, Emmanuel Tessier, Vera Slaveykova, David Amouroux
Summary: Understanding the complexation of mercury (Hg) with low molecular weight (LMW) bioligands can help elucidate its speciation, and the use of isotopically labeled Hg species in cyanobacteria has provided insights into the role of intracellular biogenic ligands in the speciation of iHg and MeHg.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Daniel A. Alfaro-Sayes, Jerome Amoah, Shimpei Aikawa, Mami Matsuda, Tomohisa Hasunuma, Akihiko Kondo, Chiaki Ogino
Summary: This study investigated the immobilization of a cyanobacterial strain for succinate production. The results showed that immobilized cells had higher succinate yield and dry cell weight compared to free cells during continuous autofermentation. In addition, the immobilization process allowed for shorter cultivation time. Continuous batch culture proved to be a convenient strategy for utilizing immobilized cyanobacteria, resulting in a simpler and faster process with increased succinate production rate in each cycle.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Takahiro Yukawa, Takahiro Bamba, Mami Matsuda, Takanobu Yoshida, Kentaro Inokuma, Jungyeon Kim, Jae Won Lee, Yong-Su Jin, Akihiko Kondo, Tomohisa Hasunuma
Summary: Efficient production of 3,4-dihydroxybutyrate from xylose by engineered yeast was achieved through genetic and environmental perturbations. The biosynthetic pathway of 3,4-dihydroxybutyrate was established by deleting ADH6 and overexpressing yneI genes in yeast. Alkaline fermentation was performed to minimize xylonate accumulation and led to the highest combined product yields compared with previous studies.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Chemistry, Physical
Si Zhao, Zaosheng Lv, Yang Lei, Yanfen Huang
Summary: In this study, a composite superhydrophobic coating capable of applying to a variety of substrates was developed, which exhibited exceptional superhydrophobicity and self-cleaning properties for the efficient separation of oil/water mixtures. The reagents and instruments used in the procedure are easily available, suggesting the potential for large-scale production.
COLLOID AND POLYMER SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Kentaro Inokuma, Shunya Miyamoto, Kohei Morinaga, Yuma Kobayashi, Ryota Kumokita, Takahiro Bamba, Yoichiro Ito, Akihiko Kondo, Tomohisa Hasunuma
Summary: In this study, a bioprocess was developed to produce 4-hydroxybenzoic acid (4-HBA) directly from cellulose using recombinant Pichia pastoris that displays heterologous cellulolytic enzymes on its cell surface. The cellulolytic enzymes, including beta-glucosidase, endoglucanase, and cellobiohydrolase, were co-displayed on the cell surface of P. pastoris. A highly 4-HBA-resistant chorismate pyruvate-lyase was also expressed in the cellulase-displaying strain. This strain achieved a 4-HBA yield of 975 mg/L from phosphoric acid swollen cellulose (PASC), which is over two times higher than the yield from glucose.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Shangjie Zhang, Feng Guo, Qiao Yang, Yujia Jiang, Shihui Yang, Jiangfeng Ma, Fengxue Xin, Tomohisa Hasunuma, Akihiko Kondo, Wenming Zhang, Min Jiang
Summary: In this study, the oleaginous yeast Yarrowia lipolytica was rationally engineered to utilize methanol and produce value-added chemicals like succinic acid. Through metabolic pathway engineering and gene regulation, the recombinant strain showed enhanced methanol utilization and cell growth. Transcriptome analysis revealed the rebalancing of methylotrophic metabolism. The study demonstrates the successful biological conversion of methanol into valuable chemicals.
Article
Biochemical Research Methods
Musashi Takekana, Takanobu Yoshida, Erika Yoshida, Sumika Ono, Shinnosuke Horie, Christopher J. Vavricka, Moe Hiratani, Kenji Tsuge, Jun Ishii, Yoshihiro Hayakawa, Akihiko Kondo, Tomohisa Hasunuma
Summary: Conventional analysis of microbial bioproducers using liquid cultures is time consuming and limits productivity. This study introduces a new method, SFE-SFC-MS/MS, which allows for direct evaluation of microbial bioproduction colonies. Compared to traditional analysis, this method offers faster and more convenient screening of highly productive strains.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Plant Sciences
Kenya Tanaka, Tomokazu Shirai, Christopher J. Vavricka, Mami Matsuda, Akihiko Kondo, Tomohisa Hasunuma
Summary: Quantitative metabolome dynamics reveal that high accumulation of glycolytic intermediates in darkness enable efficient metabolism switching for the smooth initiation of photosynthesis in cyanobacteria. Dark-to-light transitions in cyanobacteria involve drastic metabolic changes from dark respiratory metabolism to CO2 fixation through the Calvin-Benson-Bassham cycle. This study demonstrates rapid dynamic changes in metabolite concentrations and C-13 tracer incorporation after light irradiation in Synechocystis sp. PCC 6803, allowing estimation of nonstationary metabolic flux underlying CBB cycle activation. Accumulation of glycolytic intermediates is essential for efficient metabolism switching under fluctuating light environments, as indicated by the decrease in the initial oxygen evolution rate in mutants deficient in glycogen degradation or oxidative pentose phosphate pathways.
Article
Energy & Fuels
Daniel A. Alfaro-Sayes, Jerome Amoah, Nova Rachmadona, Shinji Hama, Tomohisa Hasunuma, Akihiko Kondo, Chiaki Ogino
Summary: The aim of this study was to evaluate the effect of alginate immobilization on the growth and lipid productivity of the microalgae Chlorella sorokiniana. The results showed that immobilization technology can enhance the growth and lipid productivity of microalgae.
JOURNAL OF PHYSICS-ENERGY
(2023)
Article
Multidisciplinary Sciences
Yuichi Kato, Kosuke Inabe, Yuji Haraguchi, Tatsuya Shimizu, Akihiko Kondo, Tomohisa Hasunuma
Summary: To develop a sustainable animal cell culture system, researchers introduced the lldD gene from Escherichia coli into Synechococcus sp. PCC 7002, enabling the strain to consume l-lactate. This consumption was accelerated by the expression of the lldP gene and an increase in culture temperature. The study provides new insight into the treatment of l-lactate by photosynthetic microorganisms, potentially enhancing the feasibility of animal cell culture industries.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Erina Yoshida, Yuichi Kato, Akihiko Kanamoto, Akihiko Kondo, Tomohisa Hasunuma
Summary: This study analyzed the effect of nitrogen sources on the production of fucoxanthin by OPMS 30543X microalgae. It was found that using NaNO3 as the nitrogen source resulted in higher fucoxanthin content compared to using NH4Cl. This suggests that nitrogen sources can influence the metabolic processes and biosynthesis of fucoxanthin by OPMS 30543X. The findings of this study can guide the commercial production of fucoxanthin using microalgae.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Immunology
Atomu Yamaguchi, Noriaki Maeshige, Jiawei Yan, Xiaoqi Ma, Mikiko Uemura, Mami Matsuda, Yuya Nishimura, Tomohisa Hasunuma, Hiroyo Kondo, Hidemi Fujino, Zhi-Min Yuan
Summary: This study found that myotube-derived extracellular vesicles (EVs) can suppress macrophage inflammatory responses while activating the IRG1-itaconate pathway. The miRNA composition of EVs may be related to their anti-inflammatory effects.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ryota Kumokita, Takanobu Yoshida, Tomokazu Shirai, Akihiko Kondo, Tomohisa Hasunuma
Summary: Aromatic secondary metabolites are widely used in various industries and their production currently relies on plant extraction. Microbe-based processes have recently attracted attention as sustainable alternatives. This study found that the yeast Pichia pastoris can produce higher levels of aromatic secondary metabolites from glycerol compared to glucose, and enhanced utilization of specific amino acids further increases the production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Abigail Loren Tung Uy, Atsushi Yamamoto, Mami Matsuda, Toshihiro Arae, Tomohisa Hasunuma, Taku Demura, Misato Ohtani
Summary: Carbon flow shifts from glycolysis to the shikimate pathway during xylem vessel cell differentiation, accompanied by active changes in the levels of cellulose and lignin precursors.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Mikiko Uemura, Noriaki Maeshige, Atomu Yamaguchi, Xiaoqi Ma, Mami Matsuda, Yuya Nishimura, Tomohisa Hasunuma, Taketo Inoue, Jiawei Yan, Ji Wang, Hiroyo Kondo, Hidemi Fujino
Summary: This study investigated the effect of electrical stimulation (ES) on the metabolism and inflammatory responses of macrophages. The results showed that ES had an anti-inflammatory effect by reducing inflammation and oxidative stress in macrophages, while increasing antioxidant metabolites. This suggests that ES can improve the function of macrophages.
SCIENTIFIC REPORTS
(2023)
Review
Biotechnology & Applied Microbiology
Shuai Zhao, Ting Zhang, Tomohisa Hasunuma, Akihiko Kondo, Xin-Qing Zhao, Jia-Xun Feng
Summary: This review compares the cellulase and xylanase biosynthesis in two filamentous fungi, P. oxalicum and T. reesei, and highlights the strikingly different modes of genome-wide regulation of these enzymes. The understanding of these differences may lead to improvements in fungal enzyme production through metabolic engineering and synthetic biology.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Meeting Abstract
Biotechnology & Applied Microbiology
Koichi Kitagawa, A. Ruhan, Kentaro Inokuma, Yuka Yoshigoe, Tomohisa Hasunuma, Toshiro Shirakawa
