Article
Biochemistry & Molecular Biology
Xiaoyun Wu, Jia Xu, Qiuxiang Ma, Sulaiman Ahmed, Xinlu Lu, Erjun Ling, Peng Zhang
Summary: This study reports the application of hen egg-white lysozyme (HEWL) in transgenic cassava to repress postharvest physiological deterioration. Lysozyme-expressing cassava showed lower levels of coumarins and increased tolerance of oxidative stress.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Agronomy
Feifei An, Mengjia Cui, Ting Chen, Cheng Cheng, Zhu Liu, Xiuqin Luo, Jingjing Xue, Yanqiong Tang, Jie Cai, Songbi Chen
Summary: The present study investigated the adaptive response of cassava tuberous roots under wound stress through transcriptome and metabolome analysis. The results showed that several compounds like starch, beta-carotene, (-)-epigallocatechin, cyanidin-3-O-glucoside, and L-epicatechin were altered in wounded tuberous roots. The activation of ROS scavenging system was observed at the preliminary stage of postharvest physiological deterioration (PPD). Transcriptional analysis revealed that the induced genes were mainly involved in carbohydrate metabolism, signal transduction, and polyketide metabolism. Among the differential flavonoid metabolites detected, luteolin, kaempferol, (-) epicatechin, phloretin, and naringenin were enhanced during PPD.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ruimei Li, Shuai Yuan, Yangjiao Zhou, Shijia Wang, Qin Zhou, Zhongping Ding, Yajie Wang, Yuan Yao, Jiao Liu, Jianchun Guo
Summary: This study found that RYG1 cassava tuberous roots showed delayed postharvest physiological deterioration (PPD) compared to SC8, and RYG1 roots maintained a more stable cell wall structure after storage. RNA-Seq analysis revealed transcriptome changes in tuberous roots and identified key molecular mechanisms related to PPD tolerance. GO and KEGG enrichment analysis showed that upregulated co-expressed genes in SR vs. SS were mainly enriched in photosynthesis, protein processing, hormone and cutin, suberine and wax biosynthesis, while downregulated co-expressed genes were mainly related to cell wall organization, starch and sucrose metabolism, galactose metabolism, phenylpropanoid biosynthesis, diterpenoid biosynthesis, cysteine and methionine metabolism, and flavonoid biosynthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Yan Yan, Sihan Zhao, Xiaoxue Ye, Libo Tian, Sang Shang, Weiwei Tie, Liwang Zeng, Liming Zeng, Jinghao Yang, Meiying Li, Yu Wang, Zhengnan Xie, Wei Hu
Summary: The phytohormone abscisic acid (ABA) plays a crucial role in regulating the postharvest physiological process of cassava tuberous roots. The study identified a specific ABA signaling module and elucidated its involvement in antioxidative capacity and prolonging the shelf life of cassava tuberous roots.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Applied
Congcong Wang, Yinhua Chen, Songbi Chen, Yi Min, Yanqiong Tang, Xiang Ma, Hong Li, Juanjuan Li, Zhu Liu
Summary: The aim of this study was to assess the antibacterial properties of chitosan and its effect on wound healing and resistance in cassava roots. The results showed that chitosan had a bacteriostatic effect and promoted rapid lignin accumulation, resulting in the early formation of a fracture layer. Chitosan also enhanced cassava root resistance by promoting the expression of pathogenesis-related proteins and the accumulation of flavonoids and total phenols. Overall, this study offers a novel approach for managing postharvest deterioration of cassava roots.
CARBOHYDRATE POLYMERS
(2023)
Article
Plant Sciences
Anna Vittoria Carluccio, Laure C. David, Joelle Claussen, Marco Sulley, Seun Raheemat Adeoti, Toyin Abdulsalam, Stefan Gerth, Samuel C. Zeeman, Andreas Gisel, Livia Stavolone
Summary: This study sheds light on the initial stages of root development in cassava by comparing storage roots (SR) and fibrous roots (FR). The researchers found that SR and FR follow different rhizogenic processes, with SR accumulating starch and sugars before swelling and having a distinct inner channel structure. These findings provide insights into the understanding of root development in cassava.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Biochemical Research Methods
Yan Yan, Sihan Zhao, Zehong Ding, Weiwei Tie, Wei Hu
Summary: Physiological and transcriptomic analyses revealed that the antioxidant system and kinase-mediated signaling pathway are activated during postharvest physiological deterioration (PPD) in cassava, leading to a decrease in starch content. Genes involved in starch synthesis pathway are repressed while genes associated with starch degradation pathway are induced.
PLANT MOLECULAR BIOLOGY REPORTER
(2021)
Article
Agronomy
Asunta Mukami, Bicko Steve Juma, Cecilia Mweu, Richard Oduor, Wilton Mbinda
Summary: This study employed the CRISPR-Cas9 system to mutate genes in cassava, resulting in an extended shelf life of cassava roots and improved yield stability and farmers' income.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2024)
Article
Plant Sciences
Feifei An, Xinhui Xiao, Ting Chen, Jingjing Xue, Xiuqin Luo, Wenjun Ou, Kaimian Li, Jie Cai, Songbi Chen
Summary: In this study, the bHLH gene family in cassava was investigated through genome-wide survey and transcriptome data analysis. The results revealed the important roles of these genes in various physiological and stress responses in cassava. In addition, some MebHLH genes were found to be sensitive to postharvest physiological deterioration (PPD) and may be involved in the regulation of this process.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Elizabeth Devi Wahengbam, Chingakham Premabati Devi, Susheel Kumar Sharma, Subhra Saikat Roy, Albert Maibam, Madhumita Dasgupta, Star Luikham, Tania Chongtham, Arati Ningombam, Ingudam Bhupenchandra, Laishram Kanta Singh, Yumnam Prabhabati Devi, Sushmita Thokchom, Chingakham Inao Khaba, Nameirakpam Bunindro Singh, Yallappa Rajashekar, Sudripta Das, Sansuta Mohanty, Manas Ranjan Sahoo
Summary: Rapid postharvest physiological deterioration (PPD) is a significant concern in cassava storage, with freshly harvested tubers starting to spoil within 24 to 72 hours. The accumulation of H2O2 is an early biochemical event during PPD, while the fluorescence of hydroxycoumarin compounds emitted by the cassava tubers varies significantly during storage. The total phenolics and carotenoids showed a negative correlation with PPD progression, while anthocyanin and flavonoids showed a positive correlation with ROS accumulation. The expression of PPD-associated genes was higher at 6-12 days of PPD, indicating the synthesis of ROS turnover and phenylpropanoid biosynthesis. A strong positive correlation was found between secondary metabolites and PPD signaling gene expression.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Jetsada Posom, Chutatip Duangpila, Khwantri Saengprachatanarug, Seree Wongpichet, Jiraporn Onmankhong
Summary: The study aimed to develop a new method for detecting the freshness levels of cassava roots using thermal imaging and machine learning, achieving an accuracy of 86.7% under controlled temperature conditions. Additionally, the research indicated that acceptable results could still be obtained under uncontrolled environmental conditions.
Article
Biochemistry & Molecular Biology
Getu Beyene, Raj Deepika Chauhan, Jackson Gehan, Dimuth Siritunga, Nigel Taylor
Summary: Research has found that among the five cassava isoforms, MeAPL3 plays a key role in determining storage root starch content. The degree of postharvest physiological deterioration (PPD) in storage roots is directly related to their starch content. Silencing of MeAPL3 in cassava plants through stable transgenic lines results in significantly reduced storage root starch and dry matter content, and leads to a distinct phenotype associated with increased petiole/stem angle.
PLANT MOLECULAR BIOLOGY
(2022)
Article
Immunology
Yaseen Awad-Igbaria, Shilo Dadon, Alon Shamir, Alejandro Livoff, Mark Shlapobersky, Jacob Bornstein, Eilam Palzur
Summary: This study aimed to examine the role of mast cells (MCs), nerve growth, and TRPV1 and TRPA1 in the development of provoked vulvodynia (PV). The findings suggest that vulvar hypersensitivity is mediated by the accumulation of MCs, nerve growth, and neuromdulation of TRPV1 and TRPA1. Additionally, treatment with ketotifen fumarate (KF) during the critical period of inflammation can prevent the development of chronic vulvar pain.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
Article
Plant Sciences
Huimin Peng, Haijun Hu, Keyong Xi, Xiongmeng Zhu, Jie Zhou, Junliang Yin, Fengling Guo, Yiqing Liu, Yongxing Zhu
Summary: This study investigated the use of silicon nanoparticles (SiNPs) to improve the storability of ginger rhizomes during postharvest storage and their resistance to Fusarium solani. The results showed that SiNPs increased the firmness of ginger rhizomes and reduced decay severity, water loss, total color difference, and reactive oxygen species accumulation. SiNPs treatment also modulated the expression of antioxidant enzymes, total phenolics, and flavonoid contents. Furthermore, SiNPs inhibited the growth of Fusarium solani and prevented postharvest pathogenic decay. This study provides a foundation for using SiNPs as a promising alternative tool to maintain ginger quality and control postharvest diseases.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Physics, Multidisciplinary
Michal Czakon, Alexander Mitov, Rene Poncelet
Summary: This study utilizes multijet rates to explore quantum chromodynamics (QCD), calculating NNLO QCD corrections for three-jet observables and three-to-two jet ratios. It demonstrates a significant reduction in the dependence of these observables on factorization and renormalization scales. This proof-of-principle computation represents a milestone in perturbative QCD.
PHYSICAL REVIEW LETTERS
(2021)
