Article
Biochemical Research Methods
Yu Chen, Carlos A. Tristan, Lu Chen, Vukasin M. Jovanovic, Claire Malley, Pei-Hsuan Chu, Seungmi Ryu, Tao Deng, Pinar Ormanoglu, Dingyin Tao, Yuhong Fang, Jaroslav Slamecka, Hyenjong Hong, Christopher A. LeClair, Sam Michael, Christopher P. Austin, Anton Simeonov, Ilyas Singec
Summary: Through high-throughput screening, a small-molecule cocktail called CEPT was discovered to enhance the survival of human pluripotent stem cells (hPSCs) and their differentiated progeny by simultaneously blocking multiple stress mechanisms. This cocktail showed strong improvements in various key applications in stem-cell research, providing a rationale for the safe and efficient utilization of hPSCs.
Article
Biochemistry & Molecular Biology
Mu Seog Choe, Chang Min Bae, So Jin Kim, Seung Tack Oh, Yu Jin Kown, Won-Young Choi, Ho Jae Han, Kyung Min Baek, Woochul Chang, Joong Sun Kim, Kyung Seob Lim, Seung Pil Yun, Min Young Lee
Summary: In this study, we demonstrated that yes-associated protein (YAP) plays a crucial role in maintaining self-renewal and survival of human embryonic stem cells (hESCs). We also discovered the regulatory function of doxycycline in hESCs.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell & Tissue Engineering
Chuti Laowtammathron, Chanchao Lorthongpanich, Nittaya Jiamvoraphong, Pimonwan Srisook, Phatchanat Klaihmon, Pakpoom Kheolamai, Sudjit Luanpitpong, Surapol Issaragrisil
Summary: The study explores the role of YAP in hematopoietic differentiation of hiPSCs. It is found that suppressing YAP activity enhances the efficiency of hematopoietic differentiation and increases the yield of HSPCs.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Cell & Tissue Engineering
Kevin Hayes, Yun-Kyo Kim, Martin F. Pera
Summary: Nodal is a crucial factor in mammalian embryonic development and the maintenance of pluripotent stem cells. Its signaling pathway may play an important role in regulating self-renewal in hPSC.
Article
Cell & Tissue Engineering
Marion J. Riggs, Steven D. Sheridan, Raj R. Rao
Summary: Human pluripotent stem cells (hPSCs) are prone to genomic alterations during culture, which may impact their potential in basic research and clinical applications. This study reveals that overexpression of the gene ARHGDIA at the 17q25 locus could provide a selective advantage to hPSCs, potentially explaining a common trisomy dominance. The research also presents a selection model for studying culture conditions that reduce the appearance of genomically altered hPSCs.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Cell Biology
Zhenshuo Zhu, Qin Pan, Wenxu Zhao, Xiaolong Wu, Shuai Yu, Qiaoyan Shen, Juqing Zhang, Wei Yue, Sha Peng, Na Li, Shiqiang Zhang, Anmin Lei, Jinlian Hua
Summary: The study demonstrated that overexpression of the porcine BCL2 gene significantly improved the survivability and chimeric ability of piPSCs, without compromising their pluripotency. Through digital RNA-seq analysis, it was revealed that BCL2 enhanced the expression of PI3K signal pathway receptors and promoted oxidoreductase activity and lipid metabolism, thereby maintaining the survival and pluripotency of piPSCs.
CELL PROLIFERATION
(2021)
Article
Multidisciplinary Sciences
P. Nayak, A. Colas, M. Mercola, S. Varghese, S. Subramaniam
Summary: This research compares the transcriptomic profiles of hiPSC lines of different myogenic specification levels, revealing temporal differences and the role of β-catenin transcriptional cofactors in mediating cellular interactions and external cues during differentiation.
Article
Genetics & Heredity
Shiyu An, Dan Yao, Wenyi Zhang, Hao Sun, Tianyi Yu, Ruizhe Jia, Yang Yang
Summary: This study discovered the involvement of WDR36 in self-renewal and differentiation potential of human EPS cells, with the effects being reversible by inhibiting P53.
FRONTIERS IN GENETICS
(2022)
Article
Cell & Tissue Engineering
Ying Hua, Kenji Yoshimochi, Junjun Li, Kazuhiro Takekita, Motoshi Shimotsuma, Lingjun Li, Xiang Qu, Jingbo Zhang, Yoshiki Sawa, Li Liu, Shigeru Miyagawa
Summary: In this study, a xeno-free ON2/AscleStem PSC medium was developed for the expansion of human-induced pluripotent stem cells (hiPSCs). It was found that hiPSCs cultured in this medium showed normal morphology, high self-renewal and differentiation abilities, and compatibility with different matrices. The hiPSCs grown on gelatin nanofiber showed enhanced growth and stemness. Compared to other media, ON2 medium exhibited higher pluripotency. This study demonstrates the potential of ON2 as a cost-effective and reliable medium for the long-term expansion and maintenance of hiPSCs.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Faxiang Xu, Chunhao Deng, Zhili Ren, Liangyu Sun, Ya Meng, Weiwei Liu, Jianbo Wan, Guokai Chen
Summary: Exogenous lipids such as LPA can influence the metabolism of pluripotent stem cells and induce distinct phenotypes and transcriptome profiles in the cells, altering their primed state.
Article
Hematology
Yu-Tai Chang, Ian Chiu, Qiuju Wang, Jorge Bustamante, Wenxuan Jiang, Kiera Rycaj, Song Yi, Joey Li, Jeanne Kowalski-Muegge, William Matsui
Summary: Tumor relapse and drug resistance pose challenges in the curability of multiple myeloma (MM), particularly for patients with high-risk features such as chromosome 17p13 deletion (del17p). This study reveals that the loss of p53 increases the frequency and drug resistance of tumor-initiating MM cells (TICs) through the activation of the Notch signaling pathway and upregulation of ID1 genes. The findings suggest that targeting the Notch signaling pathway with a gamma-secretase inhibitor (GSI) may be effective for treating p53 mutant MM.
Article
Genetics & Heredity
Riccardo M. Betto, Linda Diamante, Valentina Perrera, Matteo Audano, Stefania Rapelli, Andrea Lauria, Danny Incarnato, Mattia Arboit, Silvia Pedretti, Giovanni Rigoni, Vincent Guerineau, David Touboul, Giuliano Giuseppe Stirparo, Tim Lohoff, Thorsten Boroviak, Paolo Grumati, Maria E. Soriano, Jennifer Nichols, Nico Mitro, Salvatore Oliviero, Graziano Martello
Summary: This study reveals that LIF-Stat3 signaling induces genomic hypomethylation through metabolic reconfiguration, impacting the developmental plasticity of cells. Genome methylation is dynamically controlled via regulation of alpha-ketoglutarate availability or Stat3 activation. The transcription factor Otx2 is linked to DNA methylation through alpha-ketoglutarate, affecting the epigenome.
Article
Cell Biology
Elena Carbognin, Valentina Carlini, Francesco Panariello, Martina Chieregato, Elena Guerzoni, Davide Benvegnu, Valentina Perrera, Cristina Malucelli, Marcella Cesana, Antonio Grimaldi, Margherita Mutarelli, Annamaria Carissimo, Eitan Tannenbaum, Hillel Kugler, Jamie A. Hackett, Davide Cacchiarelli, Graziano Martello
Summary: Carbognin et al. found that ESRRB is essential for the activation of formative genes during the transition of naive embryonic stem cells to a formative state. Inactivation of Esrrb leads to abnormal expression of specific markers and impairs the ability of the cells to self-organize. ESRRB occupies key formative genes in both naive cells and throughout the formative state, kickstarting the formative transition and enabling unbiased multi-lineage differentiation.
NATURE CELL BIOLOGY
(2023)
Article
Cell & Tissue Engineering
Yifan Chen, Yanjie Zhou, Zhongshu Zhou, Yujiang Fang, Lin Ma, Xiaoqing Zhang, Jie Xiong, Ling Liu
Summary: This study systematically investigated the self-renewal and differentiation potency of three types of hypoimmunogenic human pluripotent stem cells (hPSCs). It was found that these cells retained their normal self-renewal capacity and three-germ-layer differentiation potency, and the derived tissue cells showed full functionality. These findings suggest that engineered hypoimmunogenic hPSCs have great potential as an unlimited universal cell source for cell therapeutics.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Developmental Biology
Masaki Kinoshita, Toshihiro Kobayashi, Benjamin Planells, Doris Klisch, Daniel Spindlow, Hideki Masaki, Susanne Bornelov, Giuliano Giuseppe Stirparo, Hitomi Matsunari, Ayuko Uchikura, Ismael Lamas-Toranzo, Jennifer Nichols, Hiromitsu Nakauchi, Hiroshi Nagashima, Ramiro Alberio, Austin Smith
Summary: This study successfully established pluripotent stem cell lines from porcine, ovine, and bovine embryos without using feeder cells or serum factors. The cells showed differentiation in teratomas and in vitro, with global transcriptome analyses revealing commonality in transcription factor expression across the three species. The research also demonstrated the generation of cloned porcine fetuses using genetically modified AFX stem cells through nuclear transfer.
Article
Engineering, Biomedical
Hyewon Jang, Dong Hyun Jo, Chang Sik Cho, Jeong Hong Shin, Jung Hwa Seo, Goosang Yu, Ramu Gopalappa, Daesik Kim, Sung-Rae Cho, Jeong Hun Kim, Hyongbum Henry Kim
Summary: Prime editing has shown the potential to precisely correct disease-causing mutations and ameliorate disease phenotypes in mouse models of genetic diseases. By identifying optimal guide RNAs, it can precisely target genes without detectable off-target edits. However, further validation in more animal models is necessary.
NATURE BIOMEDICAL ENGINEERING
(2022)
Review
Medicine, General & Internal
Jung Min Lim, Hyongbum Henry Kim
Summary: This review explores the advances and applications of CRISPR-based genome editing, explaining the basic principles and mechanisms of CRISPR-based genome editing along with recent clinical trials and animal studies. Despite some concerns over efficacy and safety, CRISPR-based genome editing holds great potential in clinical practice.
YONSEI MEDICAL JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Younggwang Kim, Seungho Lee, Soohyuk Cho, Jinman Park, Dongwoo Chae, Taeyoung Park, John D. Minna, Hyongbum Henry Kim
Summary: This study evaluates the functional effects of cancer-associated transition mutations on non-tumorigenic lung cells using base editing and sgRNA libraries. It successfully links these mutations to cellular phenotypes and finds that the majority of protein variants have neutral or likely neutral functions.
NATURE BIOTECHNOLOGY
(2022)
Article
Cell & Tissue Engineering
Eul Sik Jung, Ji Hun Kim, Mi-Yoon Chang, Wonjun Hong, Zhejiu Quan, Seung Hyun Kim, Seungkwon You, Dae-Sung Kim, Jiho Jang, Sang-Hun Lee, Hyongbum Henry Kim, Hoon Chul Kang
Summary: This study successfully performed genome editing on X-linked adrenoleukodystrophy (ALD) patient-derived iPSCs using homology-directed repair (HDR), correcting the mutation site. The edited iPSCs showed normal pluripotency marker expression, suggesting their potential use in research on the pathophysiology and therapeutics of ALD.
STEM CELL RESEARCH
(2022)
Review
Medicine, General & Internal
Hyoeun Kim, Ji Hye Koh, Jihee Lee, Yeongeun Sim, Sang-Hun Lee, Soo-Jeong Lee, Jun-Woo Ahn, Hyun-Jin Roh, Jeong Sook Kim
Summary: This case report presents a successful treatment for heterotopic cesarean scar pregnancy, using selective potassium chloride injection into the scar pregnancy and uterine cerclage to control vaginal bleeding, resulting in the preservation of the intrauterine pregnancy and full-term delivery.
Article
Chemistry, Multidisciplinary
Wookjin Shin, Sumin Jeong, Jung-uk Lee, Soo Yeun Jeong, Jeonghong Shin, Hyongbum Henry Kim, Jinwoo Cheon, Jae-Hyun Lee
Summary: This study describes a magnetogenetic method for target-specific activation of the CRISPR system in cells, using magnetomechanical force generated by magnetic nanostructures. The activated CRISPR system successfully edits the target genome, providing a versatile magnetic platform for remote gene editing in animals of different scales.
Review
Ophthalmology
Dong Hyun Jo, Sangsu Bae, Hyongbum Henry Kim, Jin-Soo Kim, Jeong Hun Kim
Summary: Inherited retinal diseases (IRDs) are vision-threatening retinal disorders caused by gene variants related to vision. Current treatment options are limited, but genome editing using CRISPR-Cas9 technologies shows promise for correcting pathogenic variants and providing new treatment opportunities.
PROGRESS IN RETINAL AND EYE RESEARCH
(2023)
Review
Medicine, General & Internal
Sang Hun Lee, Songsoo Yang, Inkyu Park, Yeong Cheol Im, Gyu Yeol Kim
Summary: This case report describes a 33-year-old pregnant woman who experienced a sudden onset of epigastric pain at 18 weeks of pregnancy due to a ruptured SAA. After emergent intervention and surgery, the woman recovered without complications and was discharged 15 days postoperatively, even though the fetus was found to be dead during resuscitation.
WORLD JOURNAL OF CLINICAL CASES
(2022)
Article
Cell Biology
Minsuk Kwak, Kaden M. Southard, Woon Ryoung Kim, Annie Lin, Nam Hyeong Kim, Ramu Gopalappa, Hyun Jung Lee, Minji An, Seo Hyun Choi, Yunmin Jung, Kunwoo Noh, Justin Farlow, Anastasios Georgakopoulos, Nikolaos K. Robakis, Min K. Kang, Matthew L. Kutys, Daeha Seo, Hyongbum Henry Kim, Yong Ho Kim, Jinwoo Cheon, Zev J. Gartner, Young-wook Jun
Summary: Adherens junctions (AJs) play a crucial role in organizing proteolytic hotspots and microdomains for the processing of transmembrane proteins such as Notch. Membrane microdomains within AJs coordinate regulated intramembrane proteolysis (RIP), while those outside AJs mediate the engagement of Notch ligand-receptor interactions. This mechanism directs the differentiation of neural progenitor cells and regulates the proteolysis of other cell-surface receptors.
NATURE CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Goosang Yu, Hui Kwon Kim, Jinman Park, Hyunjong Kwak, Yumin Cheong, Dongyoung Kim, Jiyun Kim, Jisung Kim, Hyongbum Henry Kim
Summary: In this study, the efficiency of prime editing was evaluated for a large number of pegRNAs. Computational models, DeepPrime and DeepPrime-FT, were developed to predict editing efficiencies for different prime editing systems and cell types. The study also included profiling of editing efficiencies at mismatched targets and the development of a computational model for predicting editing efficiencies at such targets. These findings and models will greatly enhance the applications of prime editing.
Article
Biotechnology & Applied Microbiology
Nahye Kim, Sungchul Choi, Sungjae Kim, Myungjae Song, Jung Hwa Seo, Seonwoo Min, Jinman Park, Sung-Rae Cho, Hyongbum Henry Kim
Summary: A deep learning model is used to predict the best base editor for specific applications. Seven base editors and nine Cas9 variants were systematically compared to determine their editing windows, outcomes, and preferred motifs. Two computational models, DeepCas9variants and DeepBE, were developed to predict the efficiency and outcomes of base editors, resulting in significantly higher efficiency for DeepBE-designed editors compared to rational design.
NATURE BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Young-hoon Kim, Nahye Kim, Ikenna Okafor, Sungchul Choi, Seonwoo Min, Joonsun Lee, Seung-Min Bae, Keunwoo Choi, Janice Choi, Vinayak Harihar, Youngho Kim, Jin-Soo Kim, Benjamin P. Kleinstiver, Jungjoon K. Lee, Taekjip Ha, Hyongbum Henry Kim
Summary: Researchers have developed an improved version of Sniper-Cas9, called Sniper2L, which combines high specificity with retained high activity for efficient and specific genome editing at a large number of target sequences.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Biochemical Research Methods
Sang-Yeon Seo, Seonwoo Min, Sungtae Lee, Jung Hwa Seo, Jinman Park, Hui Kwon Kim, Myungjae Song, Dawoon Baek, Sung-Rae Cho, Hyongbum Henry Kim
Summary: This study compares the effectiveness of 17 small Cas9s and provides guidance for researchers to select the most suitable small Cas9 for specific target sequences.
Article
Biology
Jung Hwa Seo, Jeong Hong Shin, Junwon Lee, Daesik Kim, Hye-Yeon Hwang, Bae-Geun Nam, Jinu Lee, Hyongbum Henry Kim, Sung-Rae Cho
Summary: The use of CRISPR interference (CRISPRi) can repress the expression of mutant huntingtin while preserving wild-type huntingtin, potentially delaying disease progression in Huntington's disease, which is caused by a CAG repeat expansion in the huntingtin gene.
COMMUNICATIONS BIOLOGY
(2023)
Article
Medicine, Research & Experimental
Byunghwa Noh, John Hoon Rim, Ramu Gopalappa, Haiyue Lin, Kyu Min Kim, Min Jin Kang, Heon Yung Gee, Jae Young Choi, Hyongbum Henry Kim, Jinsei Jung
Summary: In vivo gene editing targeting outer hair cells using a dual adeno-associated virus package significantly improved auditory thresholds and a new live-cell imaging technique demonstrated the enhanced activity of KCNQ4 channels after disruption of mutant allele. These findings support the development of targeted therapies for DFNA2 and the potential use of CRISPR-based gene therapy to rectify defects in outer hair cells.
