Article
Oncology
Sunwang Xu, Cen Jiang, Ruirong Lin, Xiaopeng Wang, Xiaoqiang Hu, Wei Chen, Xiangjin Chen, Tao Chen
Summary: The study demonstrated that the transcriptional repression of ELP5 in gallbladder cancer (GBC) is highly correlated with hypermethylation of the promoter. By using a sequential combination therapy of DAC and gemcitabine, the sensitivity of GBC cells to gemcitabine could be enhanced through epigenetic activation of the elongator complex, providing a potential therapeutic strategy to overcome chemotherapy resistance in GBC.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Lirui Wang, Chunlei Zhang, Yuping Hong, Xinhong Li, Tangan Li, Ang Gao, Shaojun Pan, Bin Liu, Han Jin, Daxiang Cui
Summary: The study developed a method of combinatorial delivery of epigenetic modulatory drugs through self-assembly, effectively regulating DNA methylation and histone deacetylation, ultimately inhibiting tumor cell proliferation and promoting cell apoptosis.
Review
Biochemistry & Molecular Biology
Maochao Luo, Xingyue Yang, Hai-Ning Chen, Edouard C. Nice, Canhua Huang
Summary: Colorectal cancer is a leading cause of cancer-related deaths globally, and epigenetic regulation has been shown to play an important role in CRC drug resistance. The reversibility of epigenetic alterations provides novel therapeutic strategies to overcome drug resistance.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2021)
Article
Biology
Audrey Laurent, Thierry Madigou, Maud Bizot, Marion Turpin, Gaelle Palierne, Elise Mahe, Sarah Guimard, Raphael Metivier, Stephane Avner, Christine Le Peron, Gilles Salbert
Summary: Methylation and demethylation of cytosines in DNA play a crucial role in cell-specific gene expression. By enforcing the expression of the catalytic domain of the methylcytosine dioxygenase TET2 in breast cancer cells, researchers were able to alter the cancer-specific transcription program. TET2 decreased the tumorigenic potential of cancer cells by activating and repressing a panel of genes, leading to an exacerbation of sensitivity to autophagy inducers due to the establishment of a lysosomal storage disease-like state.
LIFE SCIENCE ALLIANCE
(2022)
Article
Environmental Sciences
Gerhard P. Aigner, Pamela Nenning, Birgit Fiechtner, Maja Srut, Martina Hoeckner
Summary: This study found that the demethylating agent Aza inhibits gene activity while Cd induces MT2 gene expression, but neither has an effect on global DNA methylation levels. Thus, Cd detoxification based on MT2 induction is not related to changes in DNA methylation.
Review
Biochemistry & Molecular Biology
Andrea Maugeri, Martina Barchitta, Roberta Magnano San Lio, Giovanni Li Destri, Antonella Agodi, Guido Basile
Summary: Studies have shown that accelerated biological aging is positively associated with cancer risk and mortality, while assessing epigenetic age may help monitor individuals at risk for CRC. Additionally, aberrant epigenetic aging could potentially identify CRC patients with high recurrence risk and poor treatment response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Mathia Colwell, Nicole M. Wanner, Chelsea Drown, Melissa Drown, Dana C. Dolinoy, Christopher Faulk
Summary: This study investigated the effects of chronic low doses of DAC on the DNA methylome and gene expression in mice. The results showed that DAC exposure led to more sensitive DNA methylation changes in testes and liver, as well as dysregulated gene expression targeting non-specific pathways. However, no dose level or regimen was found to cause systemic hypomethylation in whole mice.
Article
Nanoscience & Nanotechnology
Yan Sun, Songcai Liu, Yuan Gao, Yangyang Du, Hongqiao Cai, Jian Zhang, Zhaoguo Wang, Xi Yang, Linlin Hao, Fei Yan
Summary: The study demonstrates a novel approach of combining photothermal therapy (PTT) with DNMT1 inhibitor-mediated chemotherapy for hepatocellular carcinoma (HCC) treatment. Decitabine-loaded gold nanocages with PEGylated-T7 peptide surface modification were successfully synthesized, showing selective accumulation at tumor sites and tumor cell ablation upon NIR irradiation. Activation of tumor suppressor genes silenced by PTT-induced hyperthermia and DNA hypermethylation further supports the targeted and effective synergistic PTT and chemotherapy against tumor growth.
ACS APPLIED NANO MATERIALS
(2021)
Article
Gastroenterology & Hepatology
Kristiina Rajamaki, Aurora Taira, Riku Katainen, Niko Valimaki, Anna Kuosmanen, Roosa-Maria Plaketti, Toni T. Seppala, Maarit Ahtiainen, Erkki-Ville Wirta, Emilia Vartiainen, Paivi Sulo, Janne Ravantti, Suvi Lehtipuro, Kirsi J. Granberg, Matti Nykter, Tomas Tanskanen, Ari Ristimaki, Selja Koskensalo, Laura Renkonen-Sinisalo, Anna Lepisto, Jan Bohm, Jussi Taipale, Jukka-Pekka Mecklin, Mervi Aavikko, Kimmo Palin, Lauri A. Aaltonen
Summary: By comprehensively characterizing IBD-associated tumorigenesis using multiple high-throughput approaches, distinct mechanisms of WNT pathway dysregulation were identified in IBD-CRC, which skew the tumors towards a mesenchymal subtype. Additionally, epigenetic modifications and noncoding mutations in specific genes were found in IBD-CRC, highlighting further differences compared to sCRC.
Article
Multidisciplinary Sciences
Hwayeong Cheon, Junho K. Hur, Woochang Hwang, Hee-Jin Yang, Joo-Hiuk Son
Summary: Resonant THz radiation can modify gene expression by demethylating cells, leading to decreased DNA methylation levels. This has the potential to be used in cancer treatment as it downregulates genes involved in cancer pathways.
SCIENTIFIC REPORTS
(2023)
Review
Biochemistry & Molecular Biology
Eshaan Patnaik, Chikezie Madu, Yi Lu
Summary: Epigenetics plays a crucial role in gene regulation and tumor development. DNA methylation inhibitors and histone deacetylase inhibitors can restore normal gene expression and be effective against cancer. Understanding epigenetic modifications and utilizing inhibitors offer new possibilities for cancer research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Verona Buocikova, Eleonora Marta Longhin, Eleftherios Pilalis, Chara Mastrokalou, Svetlana Miklikova, Marina Cihova, Alexandra Poturnayova, Katarina Mackova, Andrea Babelova, Lenka Trnkova, Naouale El Yamani, Congying Zheng, Ivan Rios-Mondragon, Martina Labudova, Lucia Csaderova, Kristina Mikus Kuracinova, Peter Makovicky, Lucia Kucerova, Miroslava Matuskova, Mihaela Roxana Cimpan, Maria Dusinska, Pavel Babal, Aristotelis Chatziioannou, Alena Gabelova, Elise Runden-Pran, Bozena Smolkova
Summary: This study investigates the impact of DNA methyltransferase inhibitors on the sensitivity of breast cancer cells to anthracycline antibiotics. The results demonstrate that this combination therapy can reduce tumor growth and decrease DNA methylation levels.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Pharmacology & Pharmacy
Andrea del Saz-Lara, Hatim Boughanem, Maria-Carmen Lopez de las Hazas, Carmen Crespo, Alicia Saz-Lara, Francesco Visioli, Manuel Macias-Gonzalez, Alberto Davalos
Summary: The Mediterranean diet is associated with lower incidence of cardio-vascular and cerebrovascular diseases as well as cancer. Extra virgin olive oil, with its phenolic components such as hydroxytyrosol, has been attributed with many healthful effects. Recent studies show that extra virgin olive oil and hydroxytyrosol modulate epigenetic mechanisms, specifically DNA methylation, in human tissues. In this study, colorectal cancer cells treated with hydroxytyrosol demonstrated an increase in global DNA methylation, suggesting a potential gene target for this compound.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Oncology
Li Zhang, Sijuan Tian, Minyi Zhao, Ting Yang, Shimin Quan, Lihua Song, Xiaofeng Yang
Summary: The study identified SUV39H1-DNMT1 as a crucial regulatory axis for SMAD3 in cervical cancer, where DNMT1 mediates the epigenetic modulation of SMAD3 gene by directly binding to its promoter region. Depletion of DNMT1 effectively restores the expression of SMAD3 in vitro. The findings suggest that SUV39H1-DNMT1 axis could serve as a potential therapeutic target for cervical cancer treatment.
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2021)
Article
Medicine, Research & Experimental
Erolcan Sayar, Radhika A. Patel, Ilsa M. Coleman, Martine P. Roudier, Ailin Zhang, Pallabi Mustafi, Jin-Yih Low, Brian Hanratty, Lisa S. Ang, Vipul Bhatia, Mohamed Adil, Hasim Bakbak, David A. Quigley, Michael T. Schweizer, Jessica E. Hawley, Lori Kollath, Lawrence D. True, Felix Y. Feng, Neil H. Bander, Eva Corey, John K. Lee, Colm Morrissey, Roman Gulati, Peter S. Nelson, Michael C. Haffner
Summary: Prostate-specific membrane antigen (PSMA) expression is heterogeneous in metastatic castration-resistant prostate cancer (mCRPC), with some cases showing no detectable PSMA and others showing heterogeneous expression across metastases. Loss of PSMA is associated with epigenetic changes, including CpG methylation and loss of H3K27 acetylation at the FOLH1 locus. Treatment with HDAC inhibitors can reverse this repression and restore PSMA expression. These findings provide insights into PSMA expression patterns and regulation in mCRPC, and suggest a potential therapeutic strategy using epigenetic therapies to augment PSMA levels.
Review
Genetics & Heredity
Marvin Sylvestre, Karin Tarte, David Roulois
Article
Hematology
Dilshad H. Khan, Michael Mullokandov, Yan Wu, Veronique Voisin, Marcela Gronda, Rose Hurren, Xiaoming Wang, Neil MacLean, Danny V. Jeyaraju, Yulia Jitkova, G. Wei Xu, Rob Laister, Ayesh Seneviratne, Zachary M. Blatman, Troy Ketela, Gary D. Bader, Sajid A. Marhon, Daniel D. De Carvalho, Mark D. Minden, Atan Gross, Aaron D. Schimmer
Article
Multidisciplinary Sciences
Karan J. Abraham, Negin Khosraviani, Janet N. Y. Chan, Aparna Gorthi, Anas Samman, Dorothy Y. Zhao, Miling Wang, Michael Bokros, Elva Vidya, Lauren A. Ostrowski, Roxanne Oshidari, Violena Pietrobon, Parasvi S. Patel, Arash Algouneh, Rajat Singhania, Yupeng Liu, V. Talya Yerlici, Daniel D. De Carvalho, Michael Ohh, Brendan C. Dickson, Razq Hakem, Jack F. Greenblatt, Stephen Lee, Alexander J. R. Bishop, Karim Mekhail
Article
Biochemistry & Molecular Biology
Pier Vitale Nuzzo, Jacob E. Berchuck, Keegan Korthauer, Sandor Spisak, Amin H. Nassar, Sarah Abou Alaiwi, Ankur Chakravarthy, Shu Yi Shen, Ziad Bakouny, Francesco Boccardo, John Steinharter, Gabrielle Bouchard, Catherine R. Curran, Wenting Pan, Sylvan C. Baca, Ji-Heui Seo, Gwo-Shu Mary Lee, M. Dror Michaelson, Steven L. Chang, Sushrut S. Waikar, Guru Sonpavde, Rafael A. Irizarry, Mark Pomerantz, Daniel D. De Carvalho, Toni K. Choueiri, Matthew L. Freedman
Article
Biochemistry & Molecular Biology
Farshad Nassiri, Ankur Chakravarthy, Shengrui Feng, Shu Yi Shen, Romina Nejad, Jeffrey A. Zuccato, Mathew R. Voisin, Vikas Patil, Craig Horbinski, Kenneth Aldape, Gelareh Zadeh, Daniel D. De Carvalho
Article
Oncology
Kirsty Taylor, Helen Loo Yau, Ankur Chakravarthy, Ben Wang, Shu Yi Shen, Ilias Ettayebi, Charles A. Ishak, Philippe L. Bedard, Albiruni Abdul Razak, Aaron R. Hansen, Anna Spreafico, Dave Cescon, Marcus O. Butler, Amit M. Oza, Stephanie Lheureux, Neda Stjepanovic, Brendan Van As, Sarah Boross-Harmer, Lisa Wang, Trevor J. Pugh, Pamela S. Ohashi, Lillian L. Siu, Daniel D. De Carvalho
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2020)
Article
Multidisciplinary Sciences
Parinaz Mehdipour, Sajid A. Marhon, Ilias Ettayebi, Ankur Chakravarthy, Amir Hosseini, Yadong Wang, Fabiola Attie de Castro, Helen Loo Yau, Charles Ishak, Sagi Abelson, Catherine A. O'Brien, Daniel D. De Carvalho
Article
Biochemistry & Molecular Biology
Helen Loo Yau, Emma Bell, Ilias Ettayebi, Felipe Campos de Almeida, Giselle M. Boukhaled, Shu Yi Shen, David Allard, Beatriz Morancho, Sajid A. Marhon, Charles A. Ishak, Isabela M. Gonzaga, Tiago da Silva Medina, Rajat Singhania, Ankur Chakravarthy, Raymond Chen, Parinaz Mehdipour, Sandra Pommey, Christian Klein, Gustavo P. Amarante-Mendes, David Roulois, Joaquin Arribas, John Stagg, David G. Brooks, Daniel D. De Carvalho
Summary: The study demonstrates that DNA hypomethylating agents directly modulate the anti-tumor response and effector function of CD8(+) T cells, promoting tumor infiltration and suppressing growth. This effect is achieved through the regulation of T cell activation-related transcriptional networks, ultimately enhancing the immune response against tumors.
Correction
Multidisciplinary Sciences
Parinaz Mehdipour, Sajid A. Marhon, Ilias Ettayebi, Ankur Chakravarthy, Amir Hosseini, Yadong Wang, Fabiola Attie de Castro, Helen Loo Yau, Charles Ishak, Sagi Abelson, Catherine A. O'Brien, Daniel D. De Carvalho
Article
Hematology
Erwan Dumontet, Celine Pangault, David Roulois, Matthis Desoteux, Simon Leonard, Tony Marchand, Maelle Latour, Patricia Legoix, Damarys Loew, Florent Dingli, Joelle Dulong, Erwan Flecher, Cedric Coulouarn, Guillaume Cartron, Thierry Fest, Karin Tarte
Summary: Follicular lymphoma-derived extracellular vesicles (EVs) can enhance the support of FL B-cell growth by BM-MSCs, alter gene expression, activate different signaling pathways, and potentially contribute to the formation of a permissive stromal niche in the bone marrow for FL.
Article
Immunology
Frederic Mourcin, Lea Verdiere, David Roulois, Rada Amin, Claire Lamaison, Vonick Sibut, Brice Thamphya, Celine Pangault, Celine Monvoisin, Sarah Huet, Marine Seffals, Sylvain Baulande, Fatima Mechta-Grigoriou, Patricia Legoix, Delphine Rossille, Marion Guirriec, Simon Leonard, Guillaume Cartron, Gilles Salles, Thierry Fest, Karin Tarte
Summary: The study identified three distinct human lymphoid stromal cell (LSC) subsets with different functions in healthy tissue. In lymphoma, LSCs can be reprogrammed in response to cytokines like tumor necrosis factor and transforming growth factor beta, leading to increased expression of chemokines. This bidirectional crosstalk between LSCs and malignant B cells may be a targetable axis in lymphoma.
Correction
Immunology
Frederic Mourcin, Lea Verdiere, David Roulois, Rada Amin, Claire Lamaison, Vonick Sibut, Brice Thamphya, Celine Pangault, Celine Monvoisin, Sarah Huet, Marine Seffals, Sylvain Baulande, Fatima Mechta-Grigoriou, Patricia Legoix, Delphine Rossille, Marion Guirriec, Simon Leonard, Guillaume Cartron, Gilles Salles, Thierry Fest, Karin Tarte
Article
Cell & Tissue Engineering
Joelle Dulong, Severine Loisel, Delphine Rossille, Simon Leonard, Nadege Bescher, Isabelle Bezier, Maelle Latour, Celine Monvoisin, Delphine Monnier, Nicolas Bertheuil, David Roulois, Karin Tarte
Summary: This study identified the upregulation of CD40L in adipose tissue and the overexpression of CD40 in ASCs in obese patients. The CD40-CD40L axis plays a crucial role in modulating the interaction between ASCs and neutrophils, highlighting its therapeutic potential.
Article
Biochemical Research Methods
Helen Loo Yau, Daniel D. De Carvalho
Summary: CD8+ T cells are key effector cells in the adaptive immune response, and drug treatments may impact their function. This protocol outlines robust methods for measuring the effects of drugs on the activation and cytolytic potential of primary human CD8+ T cells through ex vivo polyclonal activation and cell culture conditions.
