Article
Food Science & Technology
Chang Chng Ong, Yen-Hui Chen
Summary: Mortierella alpina was studied for lipid recovery, with different cell disruption techniques and process parameters of supercritical carbon dioxide extraction explored. High-speed homogenization was found to be the most efficient mechanical disruption technique, while 1 M HCl treatment showed similar lipid recovery to high-speed homogenization. Supercritical carbon dioxide extraction achieved a lipid recovery rate of 92.86% under specific process parameters.
Review
Biotechnology & Applied Microbiology
Lulu Chang, Haiqin Chen, Xin Tang, Jianxin Zhao, Hao Zhang, Yong Q. Chen, Wei Chen
Summary: Mortierella alpina, an oleaginous filamentous fungus, is used for industrial production of arachidonic acid due to its high lipid productivity. Understanding its metabolic characteristics and key factors governing lipid biosynthesis is essential to enhance its industrial performance. Optimisation of fermentation and metabolic engineering are key strategies to improve lipid yield.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yen-Hui Chen, Chang-Chng Ong, Ting-Yao Lin
Summary: Arachidonic acid (ARA) is an important polyunsaturated fatty acid, and Mortierella alpina shows potential for ARA production. The study demonstrated good tolerance of M. alpina to salinity and the feasibility of using taro waste hydrolysate as a substrate.
FERMENTATION-BASEL
(2022)
Article
Agriculture, Multidisciplinary
Huidan Zhang, Weijian Wan, Qiu Cui, Xiaojin Song
Summary: This study presents a modular engineering approach that systematically eliminates metabolic bottlenecks in the production of arachidonic acid (ARA). By dividing the elongase/desaturase pathway in Mortierella alpina into push and pull modules, combinatorial optimization was performed to balance the production and consumption of intermediate metabolites. The engineered strain MA5 achieved a significant increase in ARA yield, with a final titer of 4.4 g L-1 in shake-flask fermentation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Biotechnology & Applied Microbiology
Huidan Zhang, Qiu Cui, Xiaojin Song
Summary: Arachidonic acid (ARA) is a crucial omega-6 polyunsaturated fatty acid that plays essential roles in human immune, cardiovascular, and nervous systems. Microorganisms are considered as a potential alternative resource for ARA production due to limitations in existing resources. Efforts to enhance ARA production have been focused on optimizing culture medium, fermentation processes, and genetic modification, with synthetic biology methods and technologies showing promise for further increasing ARA production.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Rafaela. B. Oliveira, Diogo Robl, Jaciane L. Ienczak
Summary: The search for rich sources of polyunsaturated fatty acids (PUFAs) has led researchers to explore microbial sources, particularly single cell oil (SCO) production by filamentous fungi and yeast. The Mortierellaceae family is well-known for its PUFA-producing strains, such as Mortierella alpina which is industrially used to produce arachidonic acid (20:4 n6). This review discusses the current strategies to increase PUFA production by Mortierellaceae strains, including physiological manipulation, metabolic engineering, and process optimization.
BIOTECHNOLOGY LETTERS
(2023)
Editorial Material
Cell Biology
Joseph T. Long, Jan Lammerding
Summary: Recent studies show that progressive nuclear deformation associated with cellular confinement triggers intracellular events promoting cell contractility and migration, indicating the nucleus plays a central role as a mechanosensor.
DEVELOPMENTAL CELL
(2021)
Article
Immunology
Zhubiao Ye, Jianru Chen, Pengran Du, Qingrong Ni, Baizhang Li, Zhe Zhang, Qi Wang, Tingting Cui, Xiuli Yi, Chunying Li, Shuli Li
Summary: This study aimed to determine the differences in specific fatty acids between vitiligo patients and healthy individuals and to investigate their association with clinical features. The results showed that the serological level of alpha-linolenic acid (ALA) was increased in vitiligo patients, while the levels of arachidonic acid (ARA), arachidic acid (AA), and behenic acid were decreased. ALA levels were positively associated with the vitiligo area scoring index (VASI). Supplementation with ARA or nordihydroguaiaretic acid (NDGA) could suppress the function of CD8(+) T cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Food Science & Technology
Zahra Ghobadi, Zohreh Hamidi-Esfahani, Mohammad Hossein Azizi
Summary: This study optimized the production of arachidonic acid by Mortierella alpina CBS 754.68 on oil cakes, identifying the best conditions for fermentation and demonstrating the significant impact of variables such as time, temperature, and substrate particle size on arachidonic acid production. The research also found that aeration rate can significantly increase arachidonic acid yield in solid-state fermentation.
FOOD SCIENCE & NUTRITION
(2022)
Article
Engineering, Environmental
Marcelle Martins, Samuel Lunardi, Vanessa de Andrade Puhl, Bruno Segalla Pizzolatti, Marcus Soares
Summary: Bank filtration for water treatment is limited by clogging, with fine sand resulting in more severe biological clogging and a significant reduction in hydraulic conductivity. Factors such as grain size distribution and hydrodynamic effects play a role in biological clogging during filtration processes.
JOURNAL OF WATER PROCESS ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Chenguang Huang, Shengnan Zhou, Chen Chen, Xiaoyu Wang, Rui Ding, Yinshuang Xu, Ziwei Cheng, Zhuqing Ye, Lijun Sun, Zi-Jian Wang, Danyou Hu, Xudong Jia, Guiyang Zhang, Shan Gao
Summary: A nanocarrier has been developed to protect against myocardial ischemia/reperfusion (MI/R) injury by reducing cardiomyocyte apoptosis and improving cardiac function. The carrier efficiently delivers an anti-cryptosporidial drug and significantly reduces MI/R-induced functional deterioration and cardiomyocyte injury in experimental models.
Article
Engineering, Environmental
Yuan Pan, Sheng-Song Yu, Zhi-Chao Xiao, Yuan Min, Tian Tian, Yu-Ming Zheng, Quan-Bao Zhao, Zhi-Hua Yuan, Han-Qing Yu
Summary: Reliable and cost-effective methods for monitoring microbial activity are crucial for process control in wastewater treatment plants. This study developed an accurate and cost-effective modified DHA approach and demonstrated its feasibility through comparison with other testing methods. In addition, the sensitivity mechanisms of the DHA test were elucidated through experiments and theoretical analysis.
Article
Agriculture, Multidisciplinary
Kazuaki Yoshinaga, Haruna Ishikawa, Fumiaki Beppu, Naohiro Gotoh
Summary: The study found that dietary polyunsaturated fatty acids can pass through the blood-brain barrier and reach the brain, but most of them are stored in the brain instead of being converted into other forms.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Stefano Turolo, Alberto Edefonti, Alessandra Mazzocchi, Marie Louise Syren, William Morello, Carlo Agostoni, Giovanni Montini
Summary: Research on the role of arachidonic acid and its metabolites in kidney disease, particularly idiopathic nephrotic syndrome, is limited. These substances play a significant role in various biological processes related to kidney function, inflammation, and fibrosis. Dietary interventions to maintain balance between arachidonic acid and linoleic acid could potentially help mitigate inflammatory states seen in kidney diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Respiratory System
Leonardo Terranova, Patrizia Rise, Andrea Gramegna, Christian Pinna, Carlo Agostoni, Marie-Louise Syren, Stefano Turolo, Paola Marchisio, Francesco Amati, Stefano Aliberti, Angelo Sala, Francesco Blasi
Summary: The study found that pro-inflammatory mediators derived from arachidonic acid metabolism in sputum of bronchiectasis patients were positively associated with neutrophil elastase, Pseudomonas aeruginosa identifications, and radiological gravity index. On the other hand, pro-resolution mediators derived from docosahexaenoic acid were associated with better health status, showing an inverse correlation with radiological gravity index, bacterial infections, and sputum volume production.
RESPIRATORY RESEARCH
(2022)
Article
Agronomy
Anam Giridhar Babu, Sang Woo Kim, Dil Raj Yadav, Umyong Hyum, Mahesh Adhikari, Youn Su Lee
Article
Agronomy
Anam Giridhar Babu, Sang Woo Kim, Dil Raj Yadav, Mahesh Adhikari, Changmu Kim, Hyang Burm Lee, Youn Su Lee
Article
Agronomy
A. Giridhar Babu, Sang Woo Kim, Mahesh Adhikari, Dil Raj Yadav, Yong Hyun Um, Changmu Kim, Hyang Burm Lee, Youn Su Lee
Article
Agronomy
Mahesh Adhikari, Dil Raj Yadav, Sangwoo Kim, Yong Hyun Um, Hyung Seung Kim, Hyang Burm Lee, Youn Su Lee
Article
Agriculture, Multidisciplinary
Mahesh Adhikari, Dil Raj Yadav, Sang Woo Kim, Young Hyun Um, Hyun Seung Kim, Seong Chan Lee, Jeong Young Song, Hong Gi Kim, Youn Su Lee
PLANT PATHOLOGY JOURNAL
(2017)
Article
Agronomy
Mahesh Adhikari, Sun Kumar Gurung, Hyun Seung Kim, Setu Bazie, Hyun Gu Lee, Hyang Burm Lee, Youn Su Lee
Article
Agronomy
Dil Raj Yadav, Sang Woo Kim, Anam Giridhar Babu, Mahesh Adhikari, Changmu Kim, Hyang Burm Lee, Youn Su Lee
Article
Agronomy
A. Giridhar Babu, Sang Woo Kim, Dil Raj Yadhav, Mahesh Adhikari, Changmu Kim, Hyang Burm Lee, Youn Su Lee
Article
Agronomy
Setu Bazie Tagele, Mahesh Adhikari, Sun Kumar Gurung, Hyun Gu Lee, Sang Woo Kim, Hyun Seung Kim, Han Jun Ju, Byeong Heon Gwon, San Kosol, Hyang Burm Lee, Youn Su Lee
Article
Biotechnology & Applied Microbiology
Dil Raj Yadav, Mahesh Adhikari, Sang Woo Kim, Hyun Seung Kim, Youn Su Lee
Summary: The study shows that antagonistic rhizobacteria can effectively reduce the incidence of Fusarium wilt in lettuce and promote lettuce growth. Bacteria such as P. proteolytica and Bacillus siamensis can be used as biofertilizers and biopesticides for sustainable crop production.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Mahesh Adhikari, Sang Woo Kim, Hyun Seung Kim, Ki Young Kim, Hyo Bin Park, Ki Jung Kim, Youn Su Lee
Summary: This study investigated the bacterial structures, including the Acidovorax species community, in watermelon cultivated soils across different regions of South Korea. The results from next generation sequencing revealed a similar trend in bacterial community and diversity among the soil samples, highlighting the importance of environmental factors in shaping microbial communities. Further correlation analysis between soil parameters and dominant bacterial communities will be conducted to better understand their interactions.
PLANT PATHOLOGY JOURNAL
(2021)
Article
Biodiversity Conservation
Geon Seung Lee, Mahesh Adhikari, Jae E. Yang, Hyuck Soo Kim, Kyu Suk Han, Kean-Soo Ha, Duck Hwan Park
Summary: Improved knowledge and understanding of bacterial communities are crucial for effective crop disease management. This study investigated the relationship between bacterial communities and common scab in different potato varieties at different locations and times. The results showed that bacterial communities were more diverse across geographical locations, spatial locations, and harvesting times rather than potato varieties, suggesting no significant correlation between bacterial community richness and diversity among the varieties.
Article
Agriculture, Multidisciplinary
Duck Kyu Ryu, Mahesh Adhikari, Dong Hyuk Choi, Kyung Jin Jun, Do Hyoung Kim, Chae Ryeong Kim, Min Kyu Kang, Duck Hwan Park
Summary: Fire blight, caused by Erwinia amylovora, is a major bacterial disease in apple and pear trees, resulting in significant economic losses worldwide. This study analyzed the mechanisms and disease severity reduction efficacy of copper-based control agents (CBCAs) against E. amylovora. The results showed that copper oxychloride, copper sulfate basic, and tribasic copper sulfate at 2,000 ppm had significant reduction effects on fire blight.
PLANT PATHOLOGY JOURNAL
(2023)
Article
Agronomy
Sun Kumar Gurung, Mahesh Adhikari, Sang Woo Kim, Setu Bazie, Hyun Seung Kim, Hyun Goo Lee, San Kosol, Hyang Burm Lee, Youn Su Lee
