Article
Biotechnology & Applied Microbiology
Abdurhaman Teyib Abafogi, Tepeng Wu, Daekyu Lee, Jinyeop Lee, Gyoujin Cho, Luke P. Lee, Sungsu Park
Summary: In this study, a new type of nanoparticles (van-PDA-MNPs) that can preconcentrate multiple bacterial species from blood and improve the sensitivity of molecular diagnostics were reported.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Microbiology
C. R. Scharn, I. A. Tickler, F. C. Tenover, R. Goering
Summary: Staphylococcal cassette chromosome mec (SCCmec) is clinically and diagnostically important in staphylococci. It contains the mecA gene encoding PBP2a, as well as other antibiotic resistance genes. The stability of SCCmec is influenced by internal mobile elements and the host cell environment.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Medicine, General & Internal
Mai Okamoto, Makoto Maejima, Taichiro Goto, Takahiro Mikawa, Kazuhiro Hosaka, Yuki Nagakubo, Yosuke Hirotsu, Kenji Amemiya, Hitomi Sueki, Masao Omata
Summary: Bacteremia is a serious disease with high mortality. The introduction of the FilmArray multiplex PCR panel for blood culture identification has greatly improved the prompt identification and treatment of the disease. The use of FA significantly reduced the duration of antibiotic use and improved overall survival in patients with bacteremia.
Review
Biochemical Research Methods
Elizabeth C. Wilkirson, Kavya L. Singampalli, Jiran Li, Desh Deepak Dixit, Xue Jiang, Diego H. Gonzalez, Peter B. Lillehoj
Summary: The detection and quantification of biomarkers in blood is crucial for the early identification, diagnosis, and treatment of various diseases. Affinity-based electrochemical sensors, which offer high sensitivity, specificity, fast detection times, ease of use, and portability, are a promising approach for detecting molecular biomarkers. However, the complexity of whole blood poses challenges, requiring sample purification and sophisticated equipment. This article provides an overview of strategies employed to improve the performance of affinity-based electrochemical sensors for biomolecular detection in whole blood.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Pavel Drevinek, Jakub Hurych, Milena Antuskova, Jan Tkadlec, Jan Berousek, Zuzana Prikrylova, Jiri Bures, Jaromir Vajter, Martin Soucek, Jan Masopust, Vendula Martinkova, Jaroslava Adamkova, Veronika Hysperska, Eliska Bebrova
Summary: The T2Bacteria Panel showed a high sensitivity in detecting bloodstream infections and was able to detect pathogens 55 hours faster than standard blood culture, enabling early identification of infections and treatment adjustments, making it suitable for point-of-care testing approach.
Article
Chemistry, Multidisciplinary
Uros Zupancic, Pawan Jolly, Pedro Estrela, Despina Moschou, Donald E. Ingber
Summary: The study introduces an EC sensor platform with a nanocomposite coating that enables simultaneous detection of multiple sepsis biomarkers, showing a good correlation with clinical samples. This platform offers a sensitive and specific tool for clinical point-of-care diagnostics.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Biochemistry & Molecular Biology
Mariam Doualeh, Matthew Payne, Edward Litton, Edward Raby, Andrew Currie
Summary: Polymicrobial sepsis has worse patient outcomes than monomicrobial sepsis. Culture-dependent diagnostic techniques have low sensitivity, leading to missed identification of causative organisms. Culture-independent methods using molecular technologies show promise, but issues such as contamination and interference from host DNA need to be addressed. This review discusses the clinical significance of polymicrobial sepsis and how alternative molecular microbiology methods can be improved to detect polymicrobial infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Abhishek Kumar, Volha Skrahina, Joshua Atta, Veronika Boettcher, Nicola Hanig, Arndt Rolfs, Gabriela Oprea, Najim Ameziane
Summary: Biological material from the oral cavity is a convenient and non-invasive source for genetic diagnostics. However, it was found that these samples contain not only human DNA but also non-human DNA sequences, mainly from bacterial genomes. This study investigated the proportion and types of non-human DNA in buccal swabs and saliva samples, identifying over 30 different bacterial families. Importantly, the contamination did not affect the diagnostic yield.
FRONTIERS IN GENETICS
(2023)
Article
Microbiology
Mateusz Rzepka, Dagmara Depka, Eugenia Gospodarek-Komkowska, Tomasz Bogiel
Summary: Research shows that the level of CMV DNA is usually higher in whole blood samples compared to plasma samples. Therefore, it is important to consistently use the same type of clinical material for monitoring CMV viremia.
Article
Public, Environmental & Occupational Health
Thomas Edwards, Christopher T. Williams, Macrine Olwala, Pauline Andang'o, Walter Otieno, Grace N. Nalwa, Abimbola Akindolire, Ana I. Cubas-Atienzar, Toby Ross, Olukemi O. Tongo, Emily R. Adams, Helen Nabwera, Stephen Allen
Summary: This study aimed to explore the rates of colonisation by ESBL or carbapenemase producers in two neonatal units in West and East Africa. By collecting stool and rectal swab samples from newborns in Nigeria and Kenya and testing for ESBL and carbapenemase genes, it was found that colonisation with ESBL organisms was more common than with carbapenemase producers. These findings have important implications for clinical management and infection control.
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2023)
Article
Microbiology
T. P. McCarty, P. Cumagun, J. Meeder, D. Moates, W. S. Edwards, J. Hutchinson, R. A. Lee, S. M. Leal Jr
Summary: The GenMark Dx ePlex system enables earlier identification of antimicrobial-resistant Gram-negative bacteria causing bloodstream infections, leading to discontinuation of unnecessary antibiotics. Its performance is superior to traditional methods, providing results much earlier. In addition, it detects bacteria harboring the CTX-M gene and enables optimization of ineffective therapy.
MICROBIOLOGY SPECTRUM
(2023)
Article
Critical Care Medicine
Wolfgang Bauer, Kai Kappert, Noa Galtung, Dana Lehmann, James Wacker, Henry K. Cheng, Oliver Liesenfeld, Ljubomir Buturovic, Roland Luethy, Timothy E. Sweeney, Rudolf Tauber, Rajan Somasundaram
Summary: A new blood-based host-response classifier (IMX-BVN-2) was developed and demonstrated accuracy in determining the likelihood of bacterial and viral infections. The study showed that host-response tests have the potential to improve patient outcomes and antimicrobial stewardship by accurately detecting the causes of infections.
CRITICAL CARE MEDICINE
(2021)
Article
Multidisciplinary Sciences
Anurup Ganguli, Jongwon Lim, Ariana Mostafa, Carlos Saavedra, Archith Rayabharam, Narayana R. Aluru, Matthew Wester, Karen C. White, James Kumar, Reubin McGuffin, Ann Frederick, Enrique Valera, Rashid Bashir
Summary: In this study, we introduce a new approach to blood-based diagnostics that allows for rapid drying of large blood volumes and access to target nucleic acid through physical networks. We demonstrate single-molecule sensitivity and detect a broad spectrum of pathogens using loop-mediated isothermal amplification. Validation with clinical samples shows high sensitivity and specificity, along with a significant reduction in sample-to-result time.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemical Research Methods
Lisa A. Simms, Corey Davies, Nadeesha Jayasundara, Sumeet Sandhu, Alexander Pintara, Amorette Pretorius, Graeme R. Nimmo, Jacqueline Harper, Matthew Hiskens, Karen Smith, Sarah Boxall, Anton Lord, Raffaella Giardino, David Farlow, Diane M. Ward, Flavia Huygens
Summary: Bloodstream infections (BSIs) that progress to sepsis are a major global healthcare issue. A rapid diagnostic assay called InfectID-BSI has the potential to accurately identify the pathogens causing the infection and improve treatment outcomes.
JOURNAL OF MICROBIOLOGICAL METHODS
(2023)
Article
Chemistry, Analytical
Le Qiu, Lei Zhang, Gary L. Horowitz, Vladimir Turzhitsky, Mark F. Coughlan, Maria Glyavina, Umar Khan, Yuri N. Zakharov, Edward Vitkin, Irving Itzkan, Lev T. Perelman
Summary: The study introduces a spectroscopic method for rapid detection of bacteria in blood and identification of major bacterial species responsible for infections, avoiding the time-consuming process in traditional methods. The research shows that species-level identification can potentially be made based on approximately one thousand bacterial cells per milliliter of blood, without the need to observe entire colonies or conduct susceptibility testing.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)