Journal
METHODS AND APPLICATIONS IN FLUORESCENCE
Volume 3, Issue 1, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/2050-6120/3/1/014001
Keywords
structured illumination microscopy; superresolution; fluorescence microscopy; spatial light modulators; liquid-crystal devices
Categories
Funding
- Federal Ministry of Education and Research, Germany (BMBF) [13N13140]
Ask authors/readers for more resources
A significant improvement in acquisition speed of structured illumination microscopy (SIM) opens a new field of applications to this already well-established super-resolution method towards 3D scanning real-time imaging of living cells. We demonstrate a method of increased acquisition speed on a two-beam SIM fluorescence microscope with a lateral resolution of similar to 100 nm at a maximum raw data acquisition rate of 162 frames per second (fps) with a region of interest of 16.5 x 16.5 mu m(2), free of mechanically moving components. We use a programmable spatial light modulator (ferroelectric LCOS) which promises precise and rapid control of the excitation pattern in the sample plane. A passive Fourier filter and a segmented azimuthally patterned polarizer are used to perform structured illumination with maximum contrast. Furthermore, the free running mode in a modern sCMOS camera helps to achieve faster data acquisition.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available