Article
Microbiology
Chih-Ming Tsai, Chun-Hsien Chen, Wei-Hung Cheng, Foekje F. Stelma, Sung-Chou Li, Wei-Chen Lin
Summary: This study investigated the effects of bacteria on A. castellanii by exposing them to different culture conditions. It was found that both bacteria and the culture medium had significant effects on the amino acid composition of A. castellanii. After long-term exposure, A. castellanii achieved amino acid homeostasis by downregulating the biosynthesis of several amino acids.
Article
Immunology
Kritsada Pruksaphon, Joshua D. Nosanchuk, Patcharin Thammasit, Monsicha Pongpom, Sirida Youngchim
Summary: This study reveals the interaction between the soil amoeba Acanthamoeba castellanii and Talaromyces (Penicillium) marneffei. The amoeba rapidly ingests T. marneffei, leading to its death, and promotes the transition of conidia to the yeast form. Additionally, T. marneffei expresses virulence factors during interaction with A. castellanii. These findings indicate that soil amoebae play a role in selecting and maintaining virulence traits in T. marneffei, a clinically important dimorphic fungus in mammalian hosts.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
M. Mozammel Hoque, Parisa Noorian, Gustavo Espinoza-Vergara, Srijon Adhikary, Joyce To, Scott A. Rice, Diane McDougald
Summary: The flagellar transcriptional regulator flrA plays a key role in flagellum synthesis initiation in Vibrio cholerae. This study demonstrates that the absence of flrA leads to increased growth and survival of V. cholerae in the amoeba host Acanthamoeba castellanii, and this is associated with upregulation of genes involved in iron acquisition and amino acid biosynthesis. The availability of iron is crucial for V. cholerae survival in amoeba, and the catalases KatB and KatG confer increased tolerance to oxidative stress. These findings highlight the importance of flrA in resistance to protozoan predation and provide insights into the molecular mechanisms underlying increased survival in the environment.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Magdalena Anna Karas, Anna Turska-Szewczuk, Iwona Komaniecka, Barbara Lotocka
Summary: Multilamellar bodies (MLBs) are lysosomal organelles that are membrane-bound and play a role in cell-to-cell communication in some protozoa. However, for Acanthamoeba castellanii, similar vesicles are believed to potentially transmit pathogenic bacteria, without clear biological roles. It is important to understand the physiology of these amoebae, which are clinically significant, and analyzing the lipid composition of MLBs can provide insights into their functions.
Article
Immunology
Breven S. S. Simcox, Brooke R. R. Tomlinson, Lindsey N. N. Shaw, Kyle H. H. Rohde
Summary: Mycobacterium abscessus (Mab) is an opportunistic pathogen that primarily infects individuals with underlying pulmonary diseases. This study investigates the role of the dosRS gene in Mab's adaptation to low oxygen conditions and its global transcriptomic response.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Charmie K. Vang, Stephanie N. Dawrs, Nicole M. Oberlag, Anah E. Gilmore, Nabeeh A. Hasan, Jennifer R. Honda
Summary: Intraspecies variation between Mycobacterium abscessus subsp. abscessus isolates results in differential infectivity in immune cells and resistance to innate defenses.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Immunology
Renan Marrichi Mauch, Peter ostrup Jensen, Tavs Qvist, Mette Kolpen, Claus Moser, Tacjana Pressler, Marcos Tadeu Nolasco da Silva, Niels Hoiby, Copenhagen Study Grp Mycobacterial Infect Cyst Fibrosis
Summary: This study aimed to investigate the adaptive immune response to Mycobacterium abscessus complex (MABSC) and its cross-reactivity with Mycobacterium avium complex (MAC) and Mycobacterium bovis (BCG) in cystic fibrosis (CF) patients and non-CF controls. The results showed a similar immune response pattern in CF patients with MABSC and BCG-vaccinated controls, suggesting cross-reactivity. Importance: 6/10.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Ga-Yeong Cha, Hyejun Seo, Jaehun Oh, Byoung-Jun Kim, Bum-Joon Kim
Summary: Our recent genome-based study found that Mycobacterium paragordonae (Mpg) has evolved to adapt to intracellular living within environmental amoebae and demonstrated its enhanced survival within Acanthamoeba castellanii. We investigated the potential use of Mpg for antimycobacterial drug screening systems and found it to be more susceptible to antibiotics compared to closely-related species M. marinum (Mmar) and M. gordonae. Our two bacterial whole-cell-based drug screening systems using a recombinant Mpg strain showed promising results for discovering novel anti-mycobacterial drugs.
JOURNAL OF MICROBIOLOGY
(2023)
Article
Infectious Diseases
John Edmund Moore, Alan Murphy, Beverley Cherie Millar
Summary: The study aimed to develop a selective agar medium for isolating M. abscessus from other highly-resistant Gram-negative organisms. Although the medium showed high specificity, its sensitivity was low, making it unsuitable for primary isolation of M. abscessus from CF sputum.
INTERNATIONAL JOURNAL OF MYCOBACTERIOLOGY
(2021)
Article
Infectious Diseases
Marina da Silva Ferreira, Diego de Souza Goncalves, Elisa Goncalves Medeiros, Jose Mauro Peralta, Allan J. Guimaraes
Summary: This review discusses the interactions between free-living amoebae and fungi, exploring theories on the origin, evolution, and conservation of fungal virulence. The feast-fit-fist-feat hypothesis is proposed, suggesting that fungi may return to infect superior hosts by adapting to selective pressures from amoebae. The discussion also delves into the molecular mechanisms of amoebae attaching and internalizing fungal pathogens, and the potential implications for the emergence of more virulent fungal species through their interactions.
CURRENT TROPICAL MEDICINE REPORTS
(2021)
Article
Multidisciplinary Sciences
Daria Bottai, Wafa Frigui, Fadel Sayes, Mariagrazia Di Luca, Dalila Spadoni, Alexandre Pawlik, Marina Zoppo, Mickael Orgeur, Varun Khanna, David Hardy, Sophie Mangenot, Valerie Barbe, Claudine Medigue, Laurence Ma, Christiane Bouchier, Arianna Tavanti, Gerald Larrouy-Maumus, Roland Brosch
NATURE COMMUNICATIONS
(2020)
Article
Microbiology
Vincent Le Moigne, Clement Raynaud, Flavie Moreau, Christian Dupont, Jerome Nigou, Olivier Neyrolles, Laurent Kremer, Jean-Louis Herrmann
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2020)
Article
Infectious Diseases
J. P. Stahl, J. P. Bru, J. F. Gehanno, J. L. Herrmann, B. Castan, G. Deffontaines, A. Sotto, D. Lepelletier, P. Tattevin, N. Godefroy, E. Haddad, A. Mailles, J. P. Lavigne
MEDECINE ET MALADIES INFECTIEUSES
(2020)
Article
Chemistry, Multidisciplinary
Jerome de Ruyck, Christian Dupont, Elodie Lamy, Vincent Le Moigne, Christophe Biot, Yann Guerardel, Jean-Louis Herrmann, Mickael Blaise, Stanislas Grassin-Delyle, Laurent Kremer, Faustine Dubar
Editorial Material
Infectious Diseases
Patrick Touron, Christian Siatka, Amaury Pussiau, Sebastien Follot, Thibaud Fritz, Mikael Petit, Noussair Latifa, Jean-Louis Herrmann, Martin Rottman, Antoinette Lemoine, Sylvain Hubac
Summary: The French Gendarmerie Nationale developed a mobile DNA laboratory certified under ISO 17025 for genetic analyses, which was recently adapted to assist hospitals in Paris with high-throughput molecular screening for the SARS-CoV-2 coronavirus during the peak of the COVID-19 epidemic.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Immunology
Vincent Le Moigne, Anne-Laure Roux, Aude Jobart-Malfait, Landry Blanc, Karima Chaoui, Odile Burlet-Schiltz, Jean-Louis Gaillard, Stephane Canaan, Jerome Nigou, Jean-Louis Herrmann
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2020)
Article
Genetics & Heredity
Mickael Orgeur, Wafa Frigui, Alexandre Pawlik, Simon Clark, Ann Williams, Louis S. Ates, Laurence Ma, Christiane Bouchier, Julian Parkhill, Priscille Brodin, Roland Brosch
Summary: The study revealed genomic and phenotypic variabilities among Mycobacterium microti isolates, with clinical isolates clustering separately from vole isolates and lacking the RD1(mic) region. Clinical isolates showed higher virulence compared to vole isolates but lower virulence than M. tuberculosis H37Rv. Furthermore, the vole isolate ATCC 35872 demonstrated potential as a vaccine candidate in immunocompromised individuals.
MICROBIAL GENOMICS
(2021)
Article
Respiratory System
Vincent Le Moigne, Anne-Laure Roux, Helene Mahoudo, Gaetan Christien, Agnes Ferroni, Oana Dumitrescu, Gerard Lina, Jean-Philippe Bouchara, Patrick Plesiat, Jean-Louis Gaillard, Stephane Canaan, Genevieve Hery-Arnaud, Jean-Louis Herrmann
Summary: TLR2eF and rPLC were found to be the most efficient antigens in discriminating between NTM-culture positive and negative CF patients, with high sensitivity and specificity for detecting M. abscessus-positive CF patients.
JOURNAL OF CYSTIC FIBROSIS
(2022)
Article
Microbiology
Marion Lagune, Cecile Petit, Flor Vasquez Sotomayor, Matt D. Johansen, Kathrine S. H. Beckham, Christina Ritter, Fabienne Girard-Misguich, Matthias Wilmanns, Laurent Kremer, Florian P. Maurer, Jean-Louis Herrmann
Summary: Non-tuberculous mycobacteria (NTM) are a diverse group of microorganisms with over 200 individual species, mainly found in terrestrial and aquatic environments. In recent years, there has been increasing research on the association of NTM with infections in both immunocompetent and immunocompromised individuals. Significant developments have been made regarding the role of Type VII secretion systems (T7SS) in mycobacteria, with a focus on their impact on virulence and cell wall shaping.
Article
Biochemistry & Molecular Biology
Vincent Le Moigne, Daniela Rodriguez Rincon, Simon Glatigny, Christian M. Dupont, Christelle Langevin, Amel Ait Ali Said, Stephen A. Renshaw, R. Andres Floto, Jean-Louis Herrmann, Audrey Bernut
Summary: The study found that roscovitine has anti-inflammatory and pro-resolution effects in zebrafish, reducing neutrophil trafficking by targeting DUOX2/NADPH oxidase activity, accelerating inflammation resolution, and inducing neutrophil apoptosis and reverse migration. However, despite enhancing bacterial killing in human CF macrophages, roscovitine led to more severe infection in mouse and zebrafish models by compromising granuloma formation.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Biology
Thomas Laval, Laura Pedro-Cos, Wladimir Malaga, Laure Guenin-Mace, Alexandre Pawlik, Veronique Mayau, Hanane Yahia-Cherbal, Oceane Delos, Wafa Frigui, Justine Bertrand-Michel, Christophe Guilhot, Caroline Demangel
Summary: The research revealed that Mtb has the ability to acquire specific fatty acids through Mce1 importer protein, while infected macrophages increase the synthesis of omega 6 PUFAs during the antiviral process, but this does not affect host cells or Mtb growth.
Article
Microbiology
Vincent Le Moigne, Anne-Laure Roux, Helene Mahoudo, Gaetan Christien, Agnes Ferroni, Oana Dumitrescu, Gerard Lina, Jean-Philippe Bouchara, Patrick Plesiat, Jean-Louis Gaillard, Stephane Canaan, Genevieve Hery-Arnaud, Jean-Louis Herrmann
Summary: IgA serological assay is found to be clinically useful in the diagnosis of NTM infections, particularly Mycobacterium abscessus, in patients with cystic fibrosis.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Marion Lagune, Vincent Le Moigne, Matt D. Johansen, Flor Vasquez Sotomayor, Wassim Daher, Cecile Petit, Gina Cosentino, Laura Paulowski, Thomas Gutsmann, Matthias Wilmanns, Florian P. Maurer, Jean-Louis Herrmann, Fabienne Girard-Misguich, Laurent Kremer
Summary: ESX type VII secretion systems play an important role in pathogenicity, nutrient uptake and conjugation in mycobacteria. EsxU and EsxT are substrates of ESX-4 and form a stable heterodimer that permeabilizes artificial membranes. Deletion of the esxUT genes increases the virulence of M. abscessus in animal models.
Article
Infectious Diseases
Vincent Le Moigne, Sabine Blouquit-Laye, Aurore Desquesnes, Fabienne Girard-Misguich, Jean-Louis Herrmann
Summary: This study evaluated the colocalization of an inhaled liposomal amikacin suspension (ALIS) with intracellular Mabs, and measured its intracellular anti-Mabs activity. The results showed that ALIS can colocalize with Mabs inside cells and has antibacterial activity against intracellular Mabs, suggesting its potential use for treating Mabs pulmonary infections.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)