Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 79, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/50660
Keywords
Medicine; Issue 79; Photoreceptor Cells; Rodentia; Retinal Degeneration; Retinal Detachment; animal models; Neuroscience; ophthalmology; retina; mouse; photoreceptor cell death; retinopathy; age-related macular degeneration (AMD)
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Funding
- Bausch & Lomb Vitreoretinal Fellowship
- National Eye Institute [EY014104]
- Research to Prevent Blindness Foundation
- Lions Eye Research Fund
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Subretinal injection of sodium hyaluronate is a widely accepted method of inducing retinal detachment (RD). However, the height and duration of RD or the occurrence of subretinal hemorrhage can affect photoreceptor cell death in the detached retina. Hence, it is advantageous to create reproducible RDs without subretinal hemorrhage for evaluating photoreceptor cell death. We modified a previously reported method to create bullous and persistent RDs in a reproducible location with rare occurrence of subretinal hemorrhage. The critical step of this modified method is the creation of a self-sealing scleral incision, which can prevent leakage of sodium hyaluronate after injection into the subretinal space. To make the self-sealing scleral incision, a scleral tunnel is created, followed by scleral penetration into the choroid with a 30 G needle. Although choroidal hemorrhage may occur during this step, astriction with a surgical spear reduces the rate of choroidal hemorrhage. This method allows a more reproducible and reliable model of photoreceptor death in diseases that involve RD such as rhegmatogenous RD, retinopathy of prematurity, diabetic retinopathy, central serous chorioretinopathy, and age-related macular degeneration (AMD).
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