Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 79, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/50585
Keywords
Cellular Biology; Issue 79; Adipose Tissue; Stem Cells; Humans; Cell Biology; biology (general); enzymatic digestion; collagenase; cell isolation; Stromal Vascular Fraction (SVF); Adipose-derived Stem Cells; ASCs; lipoaspirate; liposuction
Categories
Funding
- National Institutes of Health
- NIAMS Institute
- NIDCR Institute
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In 2001, researchers at the University of California, Los Angeles, described the isolation of a new population of adult stem cells from liposuctioned adipose tissue that they initially termed Processed Lipoaspirate Cells or PLA cells. Since then, these stem cells have been renamed as Adipose-derived Stem Cells or ASCs and have gone on to become one of the most popular adult stem cells populations in the fields of stem cell research and regenerative medicine. Thousands of articles now describe the use of ASCs in a variety of regenerative animal models, including bone regeneration, peripheral nerve repair and cardiovascular engineering. Recent articles have begun to describe the myriad of uses for ASCs in the clinic. The protocol shown in this article outlines the basic procedure for manually and enzymatically isolating ASCs from large amounts of lipoaspirates obtained from cosmetic procedures. This protocol can easily be scaled up or down to accommodate the volume of lipoaspirate and can be adapted to isolate ASCs from fat tissue obtained through abdominoplasties and other similar procedures.
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