Article
Biochemistry & Molecular Biology
Yiwen Zha, Yaoyao Lu, Ting Zhang, Kunning Yan, Wenwen Zhuang, Jingyan Liang, Yong Cheng, Yingge Wang
Summary: APOC3 deficiency in rabbits delays the formation of atherosclerosis induced by a high-fat diet, making it a potential novel therapeutic target for treating atherosclerosis.
LIPIDS IN HEALTH AND DISEASE
(2021)
Article
Engineering, Biomedical
Pedro Silva-Pinheiro, Christian D. Mutti, Lindsey Van Haute, Christopher A. Powell, Pavel A. Nash, Keira Turner, Michal Minczuk
Summary: The study developed a DNA editor called MitoKO, which can precisely eliminate each protein-coding gene in the mouse mitochondrial genome. MitoKO was used to generate knockout cells with high homoplasmy levels in vitro and a mouse model with high heteroplasmy levels and no off-target edits. This tool will aid in the comprehensive investigation of mtDNA-related pathways and the creation of clinically relevant in vivo models of mtDNA dysfunction.
NATURE BIOMEDICAL ENGINEERING
(2023)
Article
Engineering, Biomedical
Nikita Mikhailov, Riikka H. Hamalainen
Summary: Mitochondria are not only the energy factories of eukaryotic cells, but also have various signaling and metabolic functions. Mutations in the mitochondrial genome are a significant contributor to human disease. Modulating the ratio between healthy and mutant mtDNA in patients' cells offers a potential therapeutic option for mtDNA diseases.
ANNALS OF BIOMEDICAL ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Robert J. Charman, Neil M. Kad
Summary: This study utilizes high-speed interferometric scattering microscopy and surface-bound DNA assay to uncover the early damage detection mechanism of UvrA. It reveals that the interaction between UvrA and DNA occurs in two phases, with a faster search state allowing UvrA to explore 99% of the E. coli genome within a single division cycle.
Article
Veterinary Sciences
Katarina Kuckova, L'ubomira Gresakova, Margareta Takacsova, Anna Kandricakova, L'ubica Chrastinova, Maria Polacikova, Adam Cieslak, Sylwester Slusarczyk, Klaudia Cobanova
Summary: The study showed that the simultaneous administration of organic zinc and thyme extract can significantly improve the antioxidant response of rabbit kidneys, providing a positive effect on the antioxidant status of growing rabbits.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Review
Genetics & Heredity
Charul Singh, Ramesh Kumar, Hansa Sehgal, Sharmista Bhati, Tripti Singhal, Gayacharan, M. S. Nimmy, Renu Yadav, Santosh Kumar A. Gupta, Naglaa Abdallah, Aladdin Hamwieh, Rajendra Kumar
Summary: Genomics and genome editing offer great opportunities for crop improvement and fundamental research. The emergence of new genome editing techniques such as zinc finger nucleases (ZFNs), homing endonucleases, transcription activator-like effector nucleases (TALENs), Base Editors (BEs), and Primer Editors (PEs) enables scientists to modulate gene expressions or create novel genes with high precision and efficiency. However, these techniques are expensive and laborious, requiring protein engineering as a prerequisite. Unlike first-generation genome editing methods, CRISPR/Cas9 is easy to construct and can theoretically target multiple locations in the genome with different guide RNAs. This study discusses the progress in genome editing tools, their applications in chickpea crop development, scientific limitations, and future perspectives for enhancing drought resistance, heat tolerance, and yield in chickpea to address global climate change, hunger, and nutritional threats.
FRONTIERS IN GENETICS
(2023)
Article
Biotechnology & Applied Microbiology
Steven C. Huhn, Yang Ou, Xiaoyan Tang, Bo Jiang, Ren Liu, Henry Lin, Zhimei Du
Summary: Chinese hamster ovary (CHO) cells are widely used for industrial therapeutic recombinant protein production, but generating high-producing clones remains a challenge. This study improved genome editing process with zinc finger nucleases (ZFNs) in the GS expression system, resulting in increased GS-knockout efficiency and enriched knockout CHO hosts with robust bioprocess traits.
BIOTECHNOLOGY PROGRESS
(2021)
Article
Genetics & Heredity
Samantha Shaltz, Sue Jinks-Robertson
Summary: Double-strand breaks in Saccharomyces cerevisiae were induced using a galactoseregulated zinc-finger nuclease designed for the Drosophila rosy locus, resulting in mutagenic repair outcomes influenced by the sequence of overhangs and the spacing between ZFN-generated nicks. Analysis showed that alterations in the spacer sequence and size affected the frequency and molecular features of repair events, highlighting the importance of overhang sequence in determining repair pathways.
Review
Genetics & Heredity
Pedro Silva-Pinheiro, Michal Minczuk
Summary: Mitochondria are controlled by both nuclear DNA and their own genome, mtDNA, with mutations in mtDNA potentially leading to devastating hereditary diseases. Recent advancements in genetic modification of mammalian mtDNA show promise in using genome editing technologies for treating mitochondrial diseases.
NATURE REVIEWS GENETICS
(2022)
Article
Pharmacology & Pharmacy
Hiromi Tsushima, Kazuyo Yamada, Daisuke Miyazawa, Takeshi Ohkubo, Makoto Michikawa, Sumiko Abe-Dohmae
Summary: Knockout mice for ABCA1, A7, and apoE showed differences in lipid concentrations, behavior, and responses to thermal stimuli compared to wild-type mice. ABCA1 knockout mice exhibited specific neural function roles and distinct physiological responses.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2021)
Review
Cell & Tissue Engineering
Wilfried A. Kues, Dharmendra Kumar, Naresh L. Selokar, Thirumala Rao Talluri
Summary: Emerging and modern gene engineering techniques, such as zinc finger nucleases, transcription activator-like effector nucleases, and CRISPR/Cas9, have rapidly advanced the application of stem cell technology in disease modeling and regenerative medicine. These genome editing tools allow for precise manipulation of genes, uncovering the genetics and etiology of diseases and offering promising therapeutic solutions for genetic mutations and incurable diseases.
CURRENT STEM CELL RESEARCH & THERAPY
(2022)
Article
Multidisciplinary Sciences
Pelin Yasar, Gizem Kars, Kerim Yavuz, Gamze Ayaz, Cerag Oguztuzun, Ecenaz Bilgen, Zeynep Suvaci, Ozguel Persil Cetinkol, Tolga Can, Mesut Muyan
Summary: The study identified that CXXC5 is mainly expressed through a core promoter containing multiple transcription start sites and associated with various transcription factors and co-regulatory proteins. ELF1 and MAZ were verified to contribute to CXXC5 expression, while the first exon of variant 2 may contain a G-quadruplex forming region that could modulate CXXC5 expression.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
P. P. Amritha, Jasmine M. Shah
Summary: This article compares different technologies for gene function identification in plants, with a focus on evaluating the CRISPR/Cas9 method. Each method has its advantages and limitations, and researchers need to choose the appropriate method based on specific purposes.
MOLECULAR GENETICS AND GENOMICS
(2021)
Review
Agriculture, Multidisciplinary
A. C. Dilger, X. Chen, L. T. Honegger, B. M. Marron, J. E. Beever
Summary: Gene-editing, especially in livestock, holds promise for growth promotion. However, progress in this field has been limited, with most studies focusing on the myostatin gene. This study successfully edited the myostatin gene in pigs using zinc-finger nucleases, but viability remains poor.
CABI AGRICULTURE & BIOSCIENCE
(2022)
Review
Cell Biology
Songjiang Wu, Ling Jiang, Li Lei, Chuhan Fu, Jinhua Huang, Yibo Hu, Yumeng Dong, Jing Chen, Qinghai Zeng
Summary: Excessive production of reactive oxygen species (ROS) can cause DNA damage, protein-DNA crosslinking, and lead to cancer, neurodegenerative diseases, and aging. G-quadruplex (G4) is a common nucleic acid structure in gene regulatory regions, and abnormalities in G4 formation and destruction affect various cellular processes. G4s are highly susceptible to oxidative damage and play important roles in aging and age-related diseases. This review summarizes the interaction between ROS and G4s and their regulatory mechanisms in aging and diseases.
CELL DEATH & DISEASE
(2023)
Article
Cardiac & Cardiovascular Systems
Tomonari Koike, Yui Koike, Dongshan Yang, Yanhong Guo, Oren Rom, Jun Song, Jie Xu, Yajie Chen, Yanli Wang, Tianqing Zhu, Minerva T. Garcia-Barrio, Jianglin Fan, Y. Eugene Chen, Jifeng Zhang
Summary: Using a novel rabbit model, the study revealed the specific roles of apoAII in lipoprotein metabolism and atherosclerosis. Rabbits with human apoAII gene insertion showed lower plasma triglycerides, higher HDL cholesterol, resistance to cholesterol-rich diet, and reduced aortic atherosclerosis compared to wild-type rabbits. These findings suggest that apoAII may serve as a potential target for the treatment of atherosclerosis.
Article
Genetics & Heredity
Dongshan Yang, Xiubin Liang, Brooke Pallas, Mark Hoenerhoff, Zhuoying Ren, Renzhi Han, Jifeng Zhang, Y. Eugene Chen, Jian-Ping Jin, Fei Sun, Jie Xu
Summary: This study successfully generated CFTR-Delta F508 rabbits using CRISPR/Cas9 technology and confirmed their germline transmission. This provides a new tool for research on cystic fibrosis models and lays the foundation for future biomedical studies.
FRONTIERS IN GENETICS
(2021)
Article
Peripheral Vascular Disease
Haizhao Yan, Manabu Niimi, Chuan Wang, Yajie Chen, Huanjin Zhou, Fumikazu Matsuhisa, Kazutoshi Nishijima, Shuji Kitajima, Bo Zhang, Hiroshi Yokomichi, Katsuyuki Nakajima, Masami Murakami, Jifeng Zhang, Y. Eugene Chen, Jianglin Fan
Summary: The study aimed to investigate whether the anti-atherogenic effect of endothelial lipase (EL) is solely dependent on its lipid-lowering effect. Results showed that under conditions of hypercholesterolemia, transgenic (Tg) rabbits with increased EL expression did not exhibit significantly different lesion areas of aortic and coronary atherosclerosis compared to non-Tg rabbits, but they did demonstrate faster clearance of Dil-labeled beta-VLDLs, suggesting that enhancement of beta-VLDL catabolism is the major mechanism for the atheroprotective effects of EL in Tg rabbits.
JOURNAL OF ATHEROSCLEROSIS AND THROMBOSIS
(2021)
Article
Endocrinology & Metabolism
Yuichiro Nishida, Kazutoshi Nishijima, Yosuke Yamada, Hiroaki Tanaka, Akiko Matsumoto, Jianglin Fan, Yoichi Uda, Hajime Tomatsu, Hiroyuki Yamamoto, Kenjiro Kami, Shuji Kitajima, Keitaro Tanaka
Summary: The study found that overexpression of LPL in rabbit skeletal muscle leads to increased activities of TCA cycle and proteinogenic amino acid metabolism pathways, contributing to the anti-obesity/anti-diabetes features. Additionally, compared to the control group, in transgenic rabbits, serum lipids and insulin resistance index were significantly lower, while total energy expenditure was higher.
Article
Multidisciplinary Sciences
Manabu Niimi, Haizhao Yan, Yajie Chen, Yao Wang, Jianglin Fan
Summary: Researchers provided a detailed protocol for isolating seven lipoprotein fractions from rabbit plasma and how to analyze apolipoproteins using SDS-PAGE and Western blotting, showing representative results of lipoprotein and apolipoprotein profiles in hyperlipidemic rabbit models. This method can serve as a standard protocol for both clinicians and basic scientists to analyze lipoprotein functions.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Review
Genetics & Heredity
Jianglin Fan, Yanli Wang, Y. Eugene Chen
Summary: Rabbits, as experimental animals, play an important role in investigating cardiovascular diseases and lipid metabolism mechanisms, especially with the advent of transgenic and knockout rabbits. The progress in genome editing technologies has expanded the applications of experimental animals, including rabbits, in various research fields.
FRONTIERS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Xiawen Yang, Zhimin Mao, Yanru Huang, Haizhao Yan, Qiaojing Yan, Jingru Hong, Jianglin Fan, Jian Yao
Summary: The study demonstrates that r-Alb effectively alleviates DSS-induced colitis through ?SH group-mediated antioxidative action. Unmodified Alb has less impact in this aspect, while complete loss of efficacy is observed after removal of ?SH groups.
Article
Medicine, General & Internal
Yu-Hong Ma, Tatsuya Yamaguchi, Tomoki Yasumura, Toru Kuno, Shoji Kobayashi, Takashi Yoshida, Takeshi Ishida, Yasuaki Ishida, Shinya Takaoka, Jiang-Lin Fan, Nobuyuki Enomoto
Summary: This case report describes the unique presentation of a pancreatic cancer patient with in situ collision of extrapancreatic malignancies (early gastric cancer and DLBCL). Innovative treatment approaches were taken, with successful outcomes including the use of endoscopic submucosal dissection for lymphomas and EUS-guided fine needle aspiration for precise diagnosis and subsequent surgery.
WORLD JOURNAL OF CLINICAL CASES
(2021)
Review
Pathology
Jianglin Fan, Teruo Watanabe
Summary: Atherosclerotic disease is a global killer caused by multiple factors. While progress has been made in understanding its pathogenesis, further research is needed to develop new treatments.
PATHOLOGY INTERNATIONAL
(2022)
Article
Medicine, General & Internal
Lu Chen, Yuan Yuan, En Takashi, Akio Kamijo, Jingyan Liang, Jianglin Fan
Summary: This study constructed models to determine the most appropriate pressure values for observing blanchable erythema and early pressure injury using the transparent disc method. The results indicate that the appropriate pressure range for light pressure may vary depending on the situation, and careful observation is required.
Article
Biochemistry & Molecular Biology
Huiwen Xu, En Takashi, Jingyan Liang, Yajie Chen, Yuan Yuan, Jianglin Fan
Summary: The study found that hydrotherapy preconditioning had a preventive effect on pressure injuries in rat models, potentially due to its upregulation of Hsp27.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Lu Chen, En Takashi, Ping Hou, Akio Kamijo, Daiji Miura, Jianglin Fan
Summary: Persistent ischemia caused by thrombi is identified as an important cause of early pressure injury deterioration post-decompression. The capillary refill time test coupled with the transparent disc method may become a promising technique for detecting ischemia post-decompression.
Article
Biochemistry & Molecular Biology
Qingqing Yu, Panpan Wei, Liran Xu, Congcong Xia, Yafeng Li, Haole Liu, Xiaojie Song, Kangli Tian, Weilai Fu, Rong Wang, Weirong Wang, Liang Bai, Jianglin Fan, Enqi Liu, Sihai Zhao
Summary: Accumulated evidence suggests that elevated levels of urotensin II (UII) are associated with cardiovascular diseases. This study investigated the role of UII in the initiation, progression, and regression of atherosclerosis. The results showed that UII promoted atherosclerotic fatty streak formation and increased plaque size in various arteries. UII also delayed the regression of atherosclerosis and had pro-angiogenic effects in the lesion, indicating its involvement in complex plaque development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Yajie Chen, Manabu Niimi, Lan Zhang, Xiangming Tang, Jian Lu, Jianglin Fan
Summary: In this study, a new telemetry sensor system was developed to continuously monitor the body temperature of rabbits. The sensor was implanted subcutaneously and the temperature changes were recorded by a personal computer. The temperature data obtained from the telemetry system was consistent with the rectal temperature measured by a digital device. The analysis of body temperature changes confirmed the reliability and usefulness of this system, both in normal conditions and when fever was induced by endotoxin.
Meeting Abstract
Endocrinology & Metabolism
Dongshan Yang, Van Phuc Nguyen, Jun Song, Xiangbo Kong, Jie Xu, Jifeng Zhang, Monte A. Del Monte, Yannis Paulus, Jess Thoene, Y. Eugene Chen
MOLECULAR GENETICS AND METABOLISM
(2022)