Article
Multidisciplinary Sciences
Marius Heitzer, Julia Brockhaus, Kristian Kniha, Felix Merkord, Florian Peters, Frank Hoelzle, Evgeny Goloborodko, Ali Modabber
Summary: The novel adhesive-based suture-free anastomosis technique, using an intraluminal stent, demonstrated comparable hydrostatic strength to sutured anastomoses in an ex vivo model. It was performed significantly faster and withstood higher pressures, showing biomechanical potential as a seamless alternative to conventional anastomoses. In vivo animal studies are needed for further validation and to confirm safety.
SCIENTIFIC REPORTS
(2021)
Article
Engineering, Biomedical
Akshat Joshi, Saswat Choudhury, Vageesh Singh Baghel, Souvik Ghosh, Sumeet Gupta, Debrupa Lahiri, G. K. Ananthasuresh, Kaushik Chatterjee
Summary: In this study, a hydrogel system with defined swelling behaviors was engineered for 4D printing. The hydrogel sheets with pre-programmed infill patterns could rapidly self-roll into tubes in vivo to serve as nerve-guiding conduits for repairing sciatic nerve defects. This research demonstrates the potential of 3D-printed hydrogels for tissue constructs and peripheral nerve repair in regenerative medicine.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Polymer Science
Guillermo Conejo-Cuevas, Leire Ruiz-Rubio, Virginia Saez-Martinez, Raul Perez-Gonzalez, Oihane Gartziandia, Amaia Huguet-Casquero, Leyre Perez-Alvarez
Summary: Spontaneously formed hydrogels are highly sought after for their potential applications in injectable or wound dressing materials without additional reactions or toxic crosslinking reagents. This study focuses on the covalent modification of hyaluronic acid (HA) and chitosan (CHI) with catechol groups, and the evaluation of their tissue adhesion and film forming capacity. The results show that the adhesiveness of HA-CA hydrogel increases with gelation, while the competitive reactions of quinone in CHI-CA hydrogel slow down tissue adhesion and impair filmogenic properties.
Article
Chemistry, Multidisciplinary
Jingxian Wu, Jaemeun Lee, Joo Young Jung, Jeong Ho Hwang, Ki-Suk Kim, Mikyung Shin, Haeshin Lee, Sun-Hyun Park
Summary: Blood vessel anastomosis by suture is a commonly used life-saving technique, but it is time-consuming and labor-intensive. This study proposes practical less-suture strategies using adhesive films for anastomosis, which reduces the number of stitches required and saves operating time. The decreased stitches also help alleviate fibrosis-mediated wall-thickening. This strategy is particularly useful for emergency situations and anastomosis of small-diameter vessels.
ADVANCED MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Qiang Yang, Jing Guo, Sen Zhang, Fucheng Guan, Yue Yu, Shi Feng, Xuecui Song, Da Bao, Xin Zhang
Summary: In this study, PVA/PEO/CHI nanofiber scaffolds were successfully prepared by incorporating chitosan into the PVA/PEO system. These scaffolds exhibited a hierarchical pore structure and elevated porosity, providing suitable space for cell growth. The addition of CHI effectively improved cell adhesion and surface wettability of the nanofiber scaffolds, and increased the breaking stress and mechanical properties.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Surgery
Nicholas C. King, Kalyn R. McGuire, Maria Bejar-Chapa, Yannick A. J. Hoftiezer, Mark A. Randolph, Jonathan M. Winograd
Summary: This study investigated the biomechanical properties of different collagen membranes used for peripheral nerve repair. The results showed that commercial noncrosslinked amnion allograft membranes can withstand physiological strain and have comparable performance to repairs with human amnion.
JOURNAL OF RECONSTRUCTIVE MICROSURGERY
(2023)
Article
Engineering, Chemical
Shun-yi Yao, Si-peng Chen, Ruo-xun Wang, Kai Zhang, Xiao-xuan Lin, Sui Mai
Summary: An antibacterial adhesive was developed by adding carboxymethyl chitosan (CMC) to prevent secondary caries on the bonding surface while ensuring bonding performance. Incorporating polyelectrolyte into the adhesive system provided a new idea for the development of antibacterial adhesives. The results showed that the adhesive containing CMC significantly reduced the amount of adherent Streptococcus mutans, and the inhibitory effect was more significant with higher CMC concentration.
INTERNATIONAL JOURNAL OF ADHESION AND ADHESIVES
(2022)
Article
Biophysics
Catia Correia, Rita O. Sousa, A. Catarina Vale, Daniela Peixoto, Tiago H. Silva, Rui L. Reis, Iva Pashkuleva, Natalia M. Alves
Summary: This study describes the development of bioadhesive membranes using marine renewable biomaterials, chitosan and collagen extracted from fish skins. The functionalization of collagen with catechol groups enhances the adhesive and mechanical properties of the membranes, leading to improved cell attachment and proliferation.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Materials Science, Multidisciplinary
Yong Hu, Yuan Xu, Bing Wang, Yun Chen, Chao Huang
Summary: A methacrylated catechol-chitosan (MCCS) adhesive hydrogel was prepared by crosslinking chitosan with 2,3,4-trihydroxybenzaldehyde and methacrylic anhydride, followed by photopolymerization. The MCCS adhesive hydrogel exhibited ideal elasticity, good mechanical properties, and improved adhesive strength. It also showed controllable degradation rate, good cell compatibility, and antibacterial activity.
JOURNAL OF MATERIALS SCIENCE
(2022)
Review
Biotechnology & Applied Microbiology
Shuai Chen, Carmen J. Gil, Liqun Ning, Linqi Jin, Lilanni Perez, Gabriella Kabboul, Martin L. Tomov, Vahid Serpooshan
Summary: This article discusses the significance of Adhesive Tissue Engineering Scaffolds (ATESs), reviewing their key characteristics and requirements, exploring mechanisms of action for securing the scaffold onto tissue, and presenting strategies for assessing adhesive properties. Current applications and future prospects of advanced ATES systems in regenerative medicine therapies are highlighted, along with key challenges in various fields of tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Polymer Science
Xiang He, Ruyue Liu, Huiqing Liu, Ruixiao Wang, Zhenhao Xi, Yixiang Lin, Jie Wang
Summary: Researchers prepared CS-TBA-Fe hydrogels by mixing chitosan with TBA and FeCl3, showing strong wet tissue adhesion and high biocompatibility. The hydrogel exhibited enhanced wet tissue adhesion strength, robust adhesion to various substrates, controllable physical properties, and antibacterial activity against E. coli.
Article
Chemistry, Applied
Shikha Kumari, Pradeep Srivastava, Abha Mishra
Summary: In this study, chitosan and gelatin-based artificial biomimetic 3D structures were developed and loaded with nanobioglass and beta tri-calcium phosphate to create potential artificial acellular bone grafts. The scaffolds exhibited porous structure, low swelling, and high mechanical properties, while promoting cell viability and osteogenesis.
JOURNAL OF POROUS MATERIALS
(2023)
Article
Chemistry, Applied
Ha Young Jung, Phuong Le Thi, Kyung-Hee HwangBo, Jin Woo Bae, Ki Dong Park
Summary: This study developed in situ forming chitosan/PEG hydrogels with improved mechanical properties and thoroughly elucidated the effect of PEG on the physico-chemical properties of the hybrid hydrogels. The resulting hydrogels demonstrated excellent hemostatic ability and high biocompatibility, suggesting great potential for biomedical applications in the near future.
CARBOHYDRATE POLYMERS
(2021)
Article
Engineering, Environmental
Hogyun Cheong, Young-Joon Jun, Eun Young Jeon, Jong In Lee, Hyun Jun Jo, Hae Yeon Park, Eunjin Kim, Jong Won Rhie, Kye Il Joo, Hyung Joon Cha
Summary: A method of seamless nerve anastomosis using a bioadhesive hydrogel was proposed in this study, which effectively promoted nerve regeneration by inducing macrophage polarization, showing significant advantages over traditional suture techniques.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Materials Science, Multidisciplinary
Kieu The Loan Trinh, Duc Anh Thai, Da Hyun Yang, Nae Yoon Lee
Summary: Researchers developed a biocompatible adhesive using a chitosan-vitamin C hydrogel for bonding poly-(methylmethacrylate) substrates. Hydrogen bonding and ionic cross-linking between the hydrogel and PMMA were identified as the main chemical interactions enabling robust bonding. The modified PMMA surfaces exhibited enhanced hydrophilicity, resulting in a maximum bond strength of 1.48 MPa and leak-proof microchannels. The adhesive was successfully applied in the on-chip culturing of mesenchymal stem cells, promoting the formation of MSC spheroids.
ACS APPLIED POLYMER MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Ashour Sliow, Zhi Ma, Gaetano Gargiulo, David Mahns, Damia Mawad, Paul Breen, Marcus Stoodley, Jessica Houang, Rhiannon Kuchel, Giuseppe C. Tettamanzi, Richard D. Tilley, Samuel J. Frost, John Morley, Leonardo Longo, Antonio Lauto
Article
Oncology
Endale G. Gebremedhn, Peter J. Shortland, David A. Mahns
JOURNAL OF ONCOLOGY
(2019)
Article
Biology
Ingvars Birznieks, Sarah McIntyre, Hanna Maria Nilsson, Saad S. Nagi, Vaughan G. Macefield, David A. Mahns, Richard M. Vickery
Article
Cell Biology
Monokesh K. Sen, Mohammed S. M. Almuslehi, Erika Gyengesi, Simon J. Myers, Peter J. Shortland, David A. Mahns, Jens R. Coorssen
Article
Immunology
Monokesh K. Sen, Mohammed S. M. Almuslehi, Jens R. Coorssen, David A. Mahns, Peter J. Shortland
BRAIN BEHAVIOR AND IMMUNITY
(2020)
Review
Behavioral Sciences
Monokesh K. Sen, David A. Mahns, Jens R. Coorssen, Peter J. Shortland
NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS
(2019)
Article
Anesthesiology
James S. Dunn, Saad S. Nagi, David A. Mahns
EUROPEAN JOURNAL OF PAIN
(2020)
Article
Neurosciences
Mohammed S. M. Almuslehi, Monokesh K. Sen, Peter J. Shortland, David A. Mahns, Jens R. Coorssen
FRONTIERS IN CELLULAR NEUROSCIENCE
(2020)
Article
Clinical Neurology
Alessandro S. Zagami, Sumaiya Shaikh, David Mahns, Geoffrey A. Lambert
Summary: The study suggests that the nucleus raphe magnus and the periaqueductal gray matter may tightly control trigeminovascular nociception, while having less control over cutaneous trigeminal sensation. These nuclei could potentially mediate migraine headaches by modifying neuronal activity in response to migraine triggers.
Article
Immunology
Monokesh K. Sen, Mohammed S. M. Almuslehi, Peter J. Shortland, Jens R. Coorssen, David A. Mahns
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Neurosciences
Rustam Asgarov, Monokesh K. Sen, Meena Mikhael, Tim Karl, Erika Gyengesi, David A. Mahns, Chandra S. Malladi, Gerald W. Munch
Summary: The study investigated the cerebellum proteome of GFAP-IL6 transgenic mice and found that changes in protein form levels associated with IL-6 overexpression may contribute to motor function deficits. Analysis revealed differentially expressed proteoforms in three classes related to metabolism, neurodegenerative processes, and protein aggregation in the cerebellum. Additionally, impaired motor skills in transgenic mice were correlated with specific proteoform expression levels.
Review
Biochemistry & Molecular Biology
Monokesh K. Sen, Mohammed S. M. Almuslehi, Peter J. Shortland, David A. Mahns, Jens R. Coorssen
Summary: This review examines proteomic studies of multiple sclerosis (MS) patients and animal models to assess their effectiveness in mimicking MS and identifying potential biomarkers. The importance of evaluating proteoforms and challenges in identifying rational biomarkers are discussed, highlighting the need for preclinical biomarkers for early diagnosis and treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Physiology
Leonit Kiriaev, Ben D. Perry, David A. Mahns, Peter J. Shortland, Asma Redwan, John W. Morley, Stewart I. Head
Summary: The study showed that high dose minocycline treatment can reduce maximal isometric force production in fast-twitch EDL and impair protein synthesis in C2C12 cultured cells. These findings are significant for future research on the efficacy of minocycline treatment in neuromuscular disorders or muscle atrophy conditions.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Neurosciences
Monokesh K. Sen, David A. Mahns, Jens R. Coorssen, Peter J. Shortland
Summary: Microglia and astrocytes play important roles in the clearance of myelin debris and remyelination in human demyelinating diseases. Recent studies have shown that up-regulation of signaling proteins in microglia facilitates effective phagocytosis of myelin debris and the release of protective mediators accelerates remyelination. However, inadequate activation or recruitment of microglia, as well as the production of toxic mediators, impairs remyelination and leads to progressive demyelination.
Article
Biochemistry & Molecular Biology
Mohammed S. M. Almuslehi, Monokesh K. Sen, Peter J. Shortland, David A. Mahns, Jens R. Coorssen
Summary: A study using histology and proteomics found that a change in visual perception is an early symptom of multiple sclerosis (MS). Although histological investigation did not detect clear pathological changes, proteomic analysis revealed significant alterations in protein forms in the optic nerve, suggesting that these proteoforms could serve as potential early markers for degenerative demyelinating conditions.
JOURNAL OF MOLECULAR NEUROSCIENCE
(2022)